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The map label for this gene is aceF [H]

Identifier: 197118631

GI number: 197118631

Start: 2618398

End: 2619840

Strand: Reverse

Name: aceF [H]

Synonym: Gbem_2250

Alternate gene names: 197118631

Gene position: 2619840-2618398 (Counterclockwise)

Preceding gene: 197118632

Following gene: 197118630

Centisome position: 56.77

GC content: 63.2

Gene sequence:

>1443_bases
ATGAACGAGATCGTCATGCCGAAACTGTCCGACACCATGACCGAGGGGAGGCTAGTCTCCTGGAAGAAGAGAGTAGGGGA
GACGGTGACCCGCGGCGAGGTGATTGCCGAGGTCGAGACCGACAAGGCGAACATGGAACTGGAAGCCTACGTCTCGGGCG
AGTTGCTTGAGATAAGGGTGCAGACTGGGGACCTGGTGCCGGTAGGAACGGTGATCGCCGTCGTCGGCAAGGCCGGTGAA
AAGGGAGCTGGCGCTACGCAGCAGTCGGCACCGGTGCCGCACGTGGAGCCTGAACCGGCAAGGCCGCAAGAGGAGGCTCC
CGCCGGACCCCCGGCCGCGCCGAAACCAGAGCCAGGTGGGGGAGAGCCTGTATCGTCAGCCGCAGAGCCGCCTGGCGCGA
AAGGGGCGAAACCTGGCACGGCCGTCGACCTTGTTCCACCGGAAGGGGAAAAACAGGCCCCGGCCGCGCCTCCCTGGCCC
GGCGCTGAAGAGTCGCAGCCTGCCGGCGAGCCGAAAGAACCGTACCGCCCGGAGTCTTTGCAGGAGGGGGCCGCCCCGAG
CGCTGAGCTTGCAACCGGAGCCGGGCGGGAAAAGGCTGCACCCATGGTCAGACGCTGTGCCCGTGAGCTGGGGGTTGATC
TCGCCCAGGTACAGGGAAGCGGTCCTGAAGGGCGCATTCTGCTCACCGACCTGAAGTTGCAAAAGAAGGAATCTGCGCCG
GCTGGTCAAGCTCCCCAGGCTGCCGCTGAAGCGGCGCCCGCGCCTCAGGGCGAGGGGCCGAGGCCGATGTCGCGGCTGCG
GAGCGCTGTCGCCAGGACGGTGACCGAATCCTGGCATAACATCCCGCACTTCACAGTCACCGTGGATGTCGAGATGGACG
AGGCTGAAGCGGTCCGGCGCCAGTTGAAGCAAGGCGGCATGCCGGTCAGCGTCAACGATCTCATCGTGAAAGCGGTGGCT
CTGGCGCTCTCACAGTTCCCGCAGCTGAACGCGAGCTTTACGCCGGAAGGGCTGCAGTTCCATAGCGACATCAACATAGC
GATAGCGGTAGGTATGTCTGATGGCGTTCTCATGCCGGTGCTCTCGGCTTGCCAGAAGCGCTCGTTGCTGGATATCGCAC
AGGAAGCAAAAAAGCTCGTGGAACGTGCCCGCTCCGGCAGCCTCAGCGAACAGGAGATGCACGGCGGGACCTTCTCCATT
TCCAACCTCGGCATGTTTGGTGTCGGTAGTTTCAGCGCCATCATCTATCCCTCCCAGTCGGGAGTACTTGCCGTCGGGAC
CGTCTCCGAAGTCGCCAGGGTAAATTCAGGCGTGCTGAACAGCACAAAGGTCATGAAGGTCACCCTTTCCGCCGACCATC
GTGTGGTTGACGGCGCCTATGCCGCACAATTTCTGGCCGGATTGAAGGAAATCCTGGAGAACCCAGTCCGGCTTCTCATC
TGA

Upstream 100 bases:

>100_bases
GCCCTATTCGCGCAAAATCGAGAAGGTCTGCATCCCGCAGGTCGAGGGGATAGTGCAGGGCGTCCGGGATCTTCTAAACG
AATCATACTGAGGAGCCGCC

Downstream 100 bases:

>100_bases
ACCTGCAACCGTCCACGTTTGTTCCTTTGCAAAGGGGGGTAGCCTGAACGCTGCCCCCTTTGCTTTGTCGCCTTCCTCGA
CTCATGCAACGTCCCTTCGC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MNEIVMPKLSDTMTEGRLVSWKKRVGETVTRGEVIAEVETDKANMELEAYVSGELLEIRVQTGDLVPVGTVIAVVGKAGE
KGAGATQQSAPVPHVEPEPARPQEEAPAGPPAAPKPEPGGGEPVSSAAEPPGAKGAKPGTAVDLVPPEGEKQAPAAPPWP
GAEESQPAGEPKEPYRPESLQEGAAPSAELATGAGREKAAPMVRRCARELGVDLAQVQGSGPEGRILLTDLKLQKKESAP
AGQAPQAAAEAAPAPQGEGPRPMSRLRSAVARTVTESWHNIPHFTVTVDVEMDEAEAVRRQLKQGGMPVSVNDLIVKAVA
LALSQFPQLNASFTPEGLQFHSDINIAIAVGMSDGVLMPVLSACQKRSLLDIAQEAKKLVERARSGSLSEQEMHGGTFSI
SNLGMFGVGSFSAIIYPSQSGVLAVGTVSEVARVNSGVLNSTKVMKVTLSADHRVVDGAYAAQFLAGLKEILENPVRLLI

Sequences:

>Translated_480_residues
MNEIVMPKLSDTMTEGRLVSWKKRVGETVTRGEVIAEVETDKANMELEAYVSGELLEIRVQTGDLVPVGTVIAVVGKAGE
KGAGATQQSAPVPHVEPEPARPQEEAPAGPPAAPKPEPGGGEPVSSAAEPPGAKGAKPGTAVDLVPPEGEKQAPAAPPWP
GAEESQPAGEPKEPYRPESLQEGAAPSAELATGAGREKAAPMVRRCARELGVDLAQVQGSGPEGRILLTDLKLQKKESAP
AGQAPQAAAEAAPAPQGEGPRPMSRLRSAVARTVTESWHNIPHFTVTVDVEMDEAEAVRRQLKQGGMPVSVNDLIVKAVA
LALSQFPQLNASFTPEGLQFHSDINIAIAVGMSDGVLMPVLSACQKRSLLDIAQEAKKLVERARSGSLSEQEMHGGTFSI
SNLGMFGVGSFSAIIYPSQSGVLAVGTVSEVARVNSGVLNSTKVMKVTLSADHRVVDGAYAAQFLAGLKEILENPVRLLI
>Mature_480_residues
MNEIVMPKLSDTMTEGRLVSWKKRVGETVTRGEVIAEVETDKANMELEAYVSGELLEIRVQTGDLVPVGTVIAVVGKAGE
KGAGATQQSAPVPHVEPEPARPQEEAPAGPPAAPKPEPGGGEPVSSAAEPPGAKGAKPGTAVDLVPPEGEKQAPAAPPWP
GAEESQPAGEPKEPYRPESLQEGAAPSAELATGAGREKAAPMVRRCARELGVDLAQVQGSGPEGRILLTDLKLQKKESAP
AGQAPQAAAEAAPAPQGEGPRPMSRLRSAVARTVTESWHNIPHFTVTVDVEMDEAEAVRRQLKQGGMPVSVNDLIVKAVA
LALSQFPQLNASFTPEGLQFHSDINIAIAVGMSDGVLMPVLSACQKRSLLDIAQEAKKLVERARSGSLSEQEMHGGTFSI
SNLGMFGVGSFSAIIYPSQSGVLAVGTVSEVARVNSGVLNSTKVMKVTLSADHRVVDGAYAAQFLAGLKEILENPVRLLI

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=295, Percent_Identity=37.9661016949153, Blast_Score=195, Evalue=7e-50,
Organism=Homo sapiens, GI203098816, Length=222, Percent_Identity=39.1891891891892, Blast_Score=168, Evalue=9e-42,
Organism=Homo sapiens, GI203098753, Length=222, Percent_Identity=39.1891891891892, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI110671329, Length=314, Percent_Identity=31.5286624203822, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=39.1304347826087, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI19923748, Length=243, Percent_Identity=29.6296296296296, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786946, Length=289, Percent_Identity=30.7958477508651, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17560088, Length=490, Percent_Identity=33.0612244897959, Blast_Score=219, Evalue=3e-57,
Organism=Caenorhabditis elegans, GI17538894, Length=320, Percent_Identity=34.0625, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17537937, Length=363, Percent_Identity=30.3030303030303, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=475, Percent_Identity=25.8947368421053, Blast_Score=116, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6324258, Length=501, Percent_Identity=30.1397205588822, Blast_Score=191, Evalue=3e-49,
Organism=Saccharomyces cerevisiae, GI6320352, Length=251, Percent_Identity=31.0756972111554, Blast_Score=126, Evalue=7e-30,
Organism=Drosophila melanogaster, GI18859875, Length=302, Percent_Identity=34.1059602649007, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI24645909, Length=218, Percent_Identity=29.3577981651376, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 50110; Mature: 50110

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEIVMPKLSDTMTEGRLVSWKKRVGETVTRGEVIAEVETDKANMELEAYVSGELLEIRV
CCCCCCCCHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCCCCEEEEEEECCEEEEEEE
QTGDLVPVGTVIAVVGKAGEKGAGATQQSAPVPHVEPEPARPQEEAPAGPPAAPKPEPGG
ECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEPVSSAAEPPGAKGAKPGTAVDLVPPEGEKQAPAAPPWPGAEESQPAGEPKEPYRPESL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
QEGAAPSAELATGAGREKAAPMVRRCARELGVDLAQVQGSGPEGRILLTDLKLQKKESAP
HHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCHHHHCCCC
AGQAPQAAAEAAPAPQGEGPRPMSRLRSAVARTVTESWHNIPHFTVTVDVEMDEAEAVRR
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHH
QLKQGGMPVSVNDLIVKAVALALSQFPQLNASFTPEGLQFHSDINIAIAVGMSDGVLMPV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEECCCCCHHHHH
LSACQKRSLLDIAQEAKKLVERARSGSLSEQEMHGGTFSISNLGMFGVGSFSAIIYPSQS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEECCCCCEEEEEECCC
GVLAVGTVSEVARVNSGVLNSTKVMKVTLSADHRVVDGAYAAQFLAGLKEILENPVRLLI
CEEEEECHHHHHHHHCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNEIVMPKLSDTMTEGRLVSWKKRVGETVTRGEVIAEVETDKANMELEAYVSGELLEIRV
CCCCCCCCHHHHHHCCHHHHHHHHHCCHHHCCCEEEEEECCCCCEEEEEEECCEEEEEEE
QTGDLVPVGTVIAVVGKAGEKGAGATQQSAPVPHVEPEPARPQEEAPAGPPAAPKPEPGG
ECCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GEPVSSAAEPPGAKGAKPGTAVDLVPPEGEKQAPAAPPWPGAEESQPAGEPKEPYRPESL
CCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
QEGAAPSAELATGAGREKAAPMVRRCARELGVDLAQVQGSGPEGRILLTDLKLQKKESAP
HHCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEECCHHHHCCCC
AGQAPQAAAEAAPAPQGEGPRPMSRLRSAVARTVTESWHNIPHFTVTVDVEMDEAEAVRR
CCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHH
QLKQGGMPVSVNDLIVKAVALALSQFPQLNASFTPEGLQFHSDINIAIAVGMSDGVLMPV
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCEEEEEECCCCCHHHHH
LSACQKRSLLDIAQEAKKLVERARSGSLSEQEMHGGTFSISNLGMFGVGSFSAIIYPSQS
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEECCCCEECCCCCEEEEEECCC
GVLAVGTVSEVARVNSGVLNSTKVMKVTLSADHRVVDGAYAAQFLAGLKEILENPVRLLI
CEEEEECHHHHHHHHCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]