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The map label for this gene is pdhB [H]

Identifier: 197118632

GI number: 197118632

Start: 2619850

End: 2620836

Strand: Reverse

Name: pdhB [H]

Synonym: Gbem_2251

Alternate gene names: 197118632

Gene position: 2620836-2619850 (Counterclockwise)

Preceding gene: 308535293

Following gene: 197118631

Centisome position: 56.79

GC content: 62.31

Gene sequence:

>987_bases
ATGCCTGAAATGACCTACCGCGACGCCATTAACCTGGCGCTTAAAGAAGAGATGCGGCGTGACAAGAAGGTGGTGGTCTA
CGGTGAAGACGTAGCCCTCTACGAGGGGGCCTTCAAGGTGACCCGCGGGCTGCTCTCCGAGTTCGGCGAGCTGAGGGTCC
GAGATTGCCCGATTTCGGAAAACACCATCGTCGGGGTGGCCGTTGGCGCCGCCATGGCTGGGGTTCGGCCGGTAGCCGAA
CTGATGACGGTAAACTTCGCGCTCCTTGCCATGGACCAGATCGTGAACCACATGGCGAAGGTCCGCTACATGTTCGGCGG
CCAGACCTCGGTCCCGATGGTGATCAGGATGCCGGGCGGCGGGGGGAGCCAGCTGGGCGCCCAGCACTCGCAGAGCCTGG
AAAGCTACTTCATGCACTGCCCCGGGATGCTTGTTGCTTATCCCGCGACCCCGGCCGACGCCAAAGGGCTCCTCAAGAGC
TCGATCCGTACCGATAACCCGGTGATATTCCTTGAGCACGAGCTTCTCTACAACAGCAAGGGAGAAGTTCCCGAAGACCC
CGAGCACCTGGTTCCGTTCGGCAAGGCCTCCATCATGCGCGCAGGCGACGCGGTGACGCTCATCGGCTACGGTCGCATGT
CCATCCTGTGCCTGCAGGCGGCGCAATTGCTCGAGAAGGAAGGCGTCTCCTGCGAGGTGATCGACCTGCGCACCCTCACC
CCGCTCGATAGCGAGACCTTCCTTAGCTCCGTGTCGAAGACCGGCCGGGCGGTCGTGGTGGAAGAGTGCTGGAGGAATGC
GGGGCTTGGGGGGGATATCGCGTCGCGCATCTACGAGCGCTGTTTCGATACCCTGCTGGCGCCGGTCAGGCGCATCTCCG
GGCTCGACGTGCCGATGCCCTATTCGCGCAAAATCGAGAAGGTCTGCATCCCGCAGGTCGAGGGGATAGTGCAGGGCGTC
CGGGATCTTCTAAACGAATCATACTGA

Upstream 100 bases:

>100_bases
TGAGATGCCACAAATCCGTTGTGATCAAGATGGAGTTTGATAATATTTTGAACTTGTGACTCTTGGTATTAATCTAAAAC
TAGCATGACTGGAGCACCAA

Downstream 100 bases:

>100_bases
GGAGCCGCCATGAACGAGATCGTCATGCCGAAACTGTCCGACACCATGACCGAGGGGAGGCTAGTCTCCTGGAAGAAGAG
AGTAGGGGAGACGGTGACCC

Product: pyruvate dehydrogenase complex, E1 protein subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPEMTYRDAINLALKEEMRRDKKVVVYGEDVALYEGAFKVTRGLLSEFGELRVRDCPISENTIVGVAVGAAMAGVRPVAE
LMTVNFALLAMDQIVNHMAKVRYMFGGQTSVPMVIRMPGGGGSQLGAQHSQSLESYFMHCPGMLVAYPATPADAKGLLKS
SIRTDNPVIFLEHELLYNSKGEVPEDPEHLVPFGKASIMRAGDAVTLIGYGRMSILCLQAAQLLEKEGVSCEVIDLRTLT
PLDSETFLSSVSKTGRAVVVEECWRNAGLGGDIASRIYERCFDTLLAPVRRISGLDVPMPYSRKIEKVCIPQVEGIVQGV
RDLLNESY

Sequences:

>Translated_328_residues
MPEMTYRDAINLALKEEMRRDKKVVVYGEDVALYEGAFKVTRGLLSEFGELRVRDCPISENTIVGVAVGAAMAGVRPVAE
LMTVNFALLAMDQIVNHMAKVRYMFGGQTSVPMVIRMPGGGGSQLGAQHSQSLESYFMHCPGMLVAYPATPADAKGLLKS
SIRTDNPVIFLEHELLYNSKGEVPEDPEHLVPFGKASIMRAGDAVTLIGYGRMSILCLQAAQLLEKEGVSCEVIDLRTLT
PLDSETFLSSVSKTGRAVVVEECWRNAGLGGDIASRIYERCFDTLLAPVRRISGLDVPMPYSRKIEKVCIPQVEGIVQGV
RDLLNESY
>Mature_327_residues
PEMTYRDAINLALKEEMRRDKKVVVYGEDVALYEGAFKVTRGLLSEFGELRVRDCPISENTIVGVAVGAAMAGVRPVAEL
MTVNFALLAMDQIVNHMAKVRYMFGGQTSVPMVIRMPGGGGSQLGAQHSQSLESYFMHCPGMLVAYPATPADAKGLLKSS
IRTDNPVIFLEHELLYNSKGEVPEDPEHLVPFGKASIMRAGDAVTLIGYGRMSILCLQAAQLLEKEGVSCEVIDLRTLTP
LDSETFLSSVSKTGRAVVVEECWRNAGLGGDIASRIYERCFDTLLAPVRRISGLDVPMPYSRKIEKVCIPQVEGIVQGVR
DLLNESY

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI156564403, Length=327, Percent_Identity=47.7064220183486, Blast_Score=318, Evalue=5e-87,
Organism=Homo sapiens, GI291084858, Length=327, Percent_Identity=45.565749235474, Blast_Score=294, Evalue=6e-80,
Organism=Homo sapiens, GI4557353, Length=326, Percent_Identity=38.0368098159509, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI34101272, Length=326, Percent_Identity=38.0368098159509, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI133778974, Length=281, Percent_Identity=28.1138790035587, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=47.8527607361963, Blast_Score=319, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI17506935, Length=325, Percent_Identity=36.3076923076923, Blast_Score=192, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17539652, Length=261, Percent_Identity=29.8850574712644, Blast_Score=68, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6319698, Length=327, Percent_Identity=43.7308868501529, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI21358145, Length=326, Percent_Identity=46.6257668711656, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24650940, Length=326, Percent_Identity=46.6257668711656, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI160714832, Length=324, Percent_Identity=35.8024691358025, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI160714828, Length=324, Percent_Identity=35.8024691358025, Blast_Score=206, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24650943, Length=91, Percent_Identity=54.9450549450549, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24650945, Length=91, Percent_Identity=54.9450549450549, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24645119, Length=252, Percent_Identity=32.5396825396825, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551847, Length=252, Percent_Identity=32.5396825396825, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45550715, Length=252, Percent_Identity=32.5396825396825, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR000089
- InterPro:   IPR011053
- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35892; Mature: 35761

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEMTYRDAINLALKEEMRRDKKVVVYGEDVALYEGAFKVTRGLLSEFGELRVRDCPISE
CCCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
NTIVGVAVGAAMAGVRPVAELMTVNFALLAMDQIVNHMAKVRYMFGGQTSVPMVIRMPGG
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GGSQLGAQHSQSLESYFMHCPGMLVAYPATPADAKGLLKSSIRTDNPVIFLEHELLYNSK
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEHHHHCCCC
GEVPEDPEHLVPFGKASIMRAGDAVTLIGYGRMSILCLQAAQLLEKEGVSCEVIDLRTLT
CCCCCCHHHHCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PLDSETFLSSVSKTGRAVVVEECWRNAGLGGDIASRIYERCFDTLLAPVRRISGLDVPMP
CCCHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSRKIEKVCIPQVEGIVQGVRDLLNESY
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PEMTYRDAINLALKEEMRRDKKVVVYGEDVALYEGAFKVTRGLLSEFGELRVRDCPISE
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHCCEEEECCCCC
NTIVGVAVGAAMAGVRPVAELMTVNFALLAMDQIVNHMAKVRYMFGGQTSVPMVIRMPGG
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCC
GGSQLGAQHSQSLESYFMHCPGMLVAYPATPADAKGLLKSSIRTDNPVIFLEHELLYNSK
CCCCCCHHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEEHHHHCCCC
GEVPEDPEHLVPFGKASIMRAGDAVTLIGYGRMSILCLQAAQLLEKEGVSCEVIDLRTLT
CCCCCCHHHHCCCCCHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEEEECC
PLDSETFLSSVSKTGRAVVVEECWRNAGLGGDIASRIYERCFDTLLAPVRRISGLDVPMP
CCCHHHHHHHHHHCCCEEEHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
YSRKIEKVCIPQVEGIVQGVRDLLNESY
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10796014; 11481430 [H]