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The map label for this gene is dctB [H]

Identifier: 197118500

GI number: 197118500

Start: 2456186

End: 2458441

Strand: Direct

Name: dctB [H]

Synonym: Gbem_2118

Alternate gene names: 197118500

Gene position: 2456186-2458441 (Clockwise)

Preceding gene: 197118499

Following gene: 197118501

Centisome position: 53.22

GC content: 59.04

Gene sequence:

>2256_bases
ATGCAACCTTCACGACTACGGGACATGCCGCTTTATAACAGCAGGATCATCAACTCCTACATCCTGCTGATAAAGCACAA
GTACAGCCACGTGGACATCTCCGAGCTGCTCCTGTACGCAGGTATGAAGGAATACGAGGTTGCCGACCAGGCGCACTGGT
TCAGCCAGGAGCAGATCGACAGGTTCCACGAGAAGCTGCAGCAGATGACCGGCAACGCGAGGATCTCGCGGGAGGCCGGG
CGCTACGCCGCATCCCCGGACGTCTTGGGGGCGATGAGGCAGTACATCCTGGGGCTAGTGGATGCCTCCAGCACCTTCGA
CATCATCAGCAAGACCACCGCCAAGTTCACCAGGTCCTCCAGCTACCAGTCGCGTTCCATTGCCTCGAACCAGGTCGAGA
TAACCGTCACCCCCTACGAGCCTGGGCTGGAGAAGCCGTACCAGTGCGAGAACAGGATCGGGTTCTTCGAGGCGATCGTG
CTCGTCTTCAACCACAAGATGACCGACCTGCAGTACTTCCCCGAGATACGCAACCTCCCCACCATCGAGCATCCCGAATG
CATGTTCAGAGGGGATCCGGTCTGCCGCTACATCGTCACCTGGGAGAAGACCCTTTTCACCTTCCTGAAAAAGATGAGGA
ACCTCCTGGCACTCCCCCTTGCTGCGTTGAACCTGGCGCTTTTGGCTATGGGGGATACGGGTGTTTTGACCTGGGTCTTT
CCGTCGAGCCTCTGCGTTTTGCTCCTCGTTGCCCTGGCGACGGAGAGCAGCGAGAAAAAGGCGATCAAGGAGAGCCTTTG
GAGCACCCGTGACTCCATCGAAAACCTCCTGGACCAGATCAACCTCAACTACAACAACGCCATGCTCACCCATGAGATCG
GGCAAAGGCTCGGCAACTACACCCGGATCGAGGAGATGCTCTTCGACGTGGCCCAGATCATGAGGTACCGGCTGGAATAC
GACCGCGGCATGATCTTTTTGGCGGACGGCGGCAGAAAGCGCCTGGAGCTGCGCGCAAGCTACGGCTACAAGGACGAGGA
ACTGGCATGCCTCAACTCGCTCCCGTTCCTGCTGGAAAACCGGGAGCTGGGGGACGTTTACCTCGAATGCTTCCGGGGGC
AGCGGCCGTTTCTGGTGAACGAGCTGTCCAGCGGCAGCGTGGGCGAAAACACCCTCGCCTGCGCCCACATGAGCGGCACG
CGCGCCTTCATCTGCTGCCCCATCGTTGCCGACGGCGCTTCCCTCGGGGTTCTGACCGTGGAAAACGTGCAGGTGAAAAG
GCCGCTGGTGGAAAGGGACATCAGCCTGATCATGGGTACCGCCTCGGTGCTAGGGATCAGCATCCGCAACTGCGAGTTGA
TCGGGGCGCTGGAAACGGCGAACGAAGAGTTGGAACTGAGGGTGGCCAAGAGAACGCAGGACCTGGAGAAAAGCCGCCAG
AAGATGCAAGAGCAGCACGAGGAACTGGTGCGGACCTATTTCGACCTGGAGGAGGAGACCGCGCAGCGGTTGAACGCGCT
GGAGGAGCTGGCGCGCAAGGAACGGATGCTTTTGCAGCAAAACCGGCTGGCGGCTCTCGGCGAGATGATCAACAACATCG
CGCACCAGTGGCGCCAGCCCCTGAACGAACTGGGGCTGATCGTCCAGGAACTTCCCATCATGTACGACCGGGGGGACTTC
AACAAGGAGTACCTGCGCGAAAGCGTCGCCAAATTCATGAAGGTTTTGAGCCACACCTCGAAGACCATCGACGACTTCAG
GACCTTCTTCAAGCCGGACCGGGAGATGGTCCCCTTCCGGGTCACCGAGGTGGTGGATAAAGCGCTCTCCCTGGTCGCGG
AGAGTCTGAAGCACCTGGAGATAAAGGTGACGGTCCATTCCGTCGACGACCCGGCTATCATGGGGCACCCAAACGAGTTC
TCCCAGGCGATCCTCAACATCCTCTTCAACGCCCGCGACGCCTTCAAAGAGCGTAGCATCTCTTGCCGGCAGATCGAGAT
CCGCATCTTCCTGGAAGACGAAACCGCCGTAGTTACCATAGGCGACAATGCCGGCGGCATCCCCGAGGAGATCATGGACA
AAATATTCGATCCCTATTTCACCACCCGCGGACCGGAGCAGGGGACCGGGATCGGCCTCTATATGACCAAGATGATCATC
GAGAAAAACATCCCCGGGAAACTGTCGGTGCGCAACACGGAAAAGGGGGCGGAATTCAGGATCGAGGTGGGCAAAGTTGC
GTCCCGCCAGCATTGA

Upstream 100 bases:

>100_bases
CTGGGTGCTGGACCTGCAGTACAGCGACGACTACCTTGAATACTGCGAGGGGCTGTTCAGGACCGGGAAAAAGGGTGCCT
GACGACTGGCCGGAGCTTCG

Downstream 100 bases:

>100_bases
CATGGTTTCCCATGCGGTTAGAATAAGTGCGGATTAATTTTAAGCGTTAGTACCTATTAATAACGCTTTTCCACTGGCCG
GGCCGGCACGGATATTCGAA

Product: sensor ATPase, GAF domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 751; Mature: 751

Protein sequence:

>751_residues
MQPSRLRDMPLYNSRIINSYILLIKHKYSHVDISELLLYAGMKEYEVADQAHWFSQEQIDRFHEKLQQMTGNARISREAG
RYAASPDVLGAMRQYILGLVDASSTFDIISKTTAKFTRSSSYQSRSIASNQVEITVTPYEPGLEKPYQCENRIGFFEAIV
LVFNHKMTDLQYFPEIRNLPTIEHPECMFRGDPVCRYIVTWEKTLFTFLKKMRNLLALPLAALNLALLAMGDTGVLTWVF
PSSLCVLLLVALATESSEKKAIKESLWSTRDSIENLLDQINLNYNNAMLTHEIGQRLGNYTRIEEMLFDVAQIMRYRLEY
DRGMIFLADGGRKRLELRASYGYKDEELACLNSLPFLLENRELGDVYLECFRGQRPFLVNELSSGSVGENTLACAHMSGT
RAFICCPIVADGASLGVLTVENVQVKRPLVERDISLIMGTASVLGISIRNCELIGALETANEELELRVAKRTQDLEKSRQ
KMQEQHEELVRTYFDLEEETAQRLNALEELARKERMLLQQNRLAALGEMINNIAHQWRQPLNELGLIVQELPIMYDRGDF
NKEYLRESVAKFMKVLSHTSKTIDDFRTFFKPDREMVPFRVTEVVDKALSLVAESLKHLEIKVTVHSVDDPAIMGHPNEF
SQAILNILFNARDAFKERSISCRQIEIRIFLEDETAVVTIGDNAGGIPEEIMDKIFDPYFTTRGPEQGTGIGLYMTKMII
EKNIPGKLSVRNTEKGAEFRIEVGKVASRQH

Sequences:

>Translated_751_residues
MQPSRLRDMPLYNSRIINSYILLIKHKYSHVDISELLLYAGMKEYEVADQAHWFSQEQIDRFHEKLQQMTGNARISREAG
RYAASPDVLGAMRQYILGLVDASSTFDIISKTTAKFTRSSSYQSRSIASNQVEITVTPYEPGLEKPYQCENRIGFFEAIV
LVFNHKMTDLQYFPEIRNLPTIEHPECMFRGDPVCRYIVTWEKTLFTFLKKMRNLLALPLAALNLALLAMGDTGVLTWVF
PSSLCVLLLVALATESSEKKAIKESLWSTRDSIENLLDQINLNYNNAMLTHEIGQRLGNYTRIEEMLFDVAQIMRYRLEY
DRGMIFLADGGRKRLELRASYGYKDEELACLNSLPFLLENRELGDVYLECFRGQRPFLVNELSSGSVGENTLACAHMSGT
RAFICCPIVADGASLGVLTVENVQVKRPLVERDISLIMGTASVLGISIRNCELIGALETANEELELRVAKRTQDLEKSRQ
KMQEQHEELVRTYFDLEEETAQRLNALEELARKERMLLQQNRLAALGEMINNIAHQWRQPLNELGLIVQELPIMYDRGDF
NKEYLRESVAKFMKVLSHTSKTIDDFRTFFKPDREMVPFRVTEVVDKALSLVAESLKHLEIKVTVHSVDDPAIMGHPNEF
SQAILNILFNARDAFKERSISCRQIEIRIFLEDETAVVTIGDNAGGIPEEIMDKIFDPYFTTRGPEQGTGIGLYMTKMII
EKNIPGKLSVRNTEKGAEFRIEVGKVASRQH
>Mature_751_residues
MQPSRLRDMPLYNSRIINSYILLIKHKYSHVDISELLLYAGMKEYEVADQAHWFSQEQIDRFHEKLQQMTGNARISREAG
RYAASPDVLGAMRQYILGLVDASSTFDIISKTTAKFTRSSSYQSRSIASNQVEITVTPYEPGLEKPYQCENRIGFFEAIV
LVFNHKMTDLQYFPEIRNLPTIEHPECMFRGDPVCRYIVTWEKTLFTFLKKMRNLLALPLAALNLALLAMGDTGVLTWVF
PSSLCVLLLVALATESSEKKAIKESLWSTRDSIENLLDQINLNYNNAMLTHEIGQRLGNYTRIEEMLFDVAQIMRYRLEY
DRGMIFLADGGRKRLELRASYGYKDEELACLNSLPFLLENRELGDVYLECFRGQRPFLVNELSSGSVGENTLACAHMSGT
RAFICCPIVADGASLGVLTVENVQVKRPLVERDISLIMGTASVLGISIRNCELIGALETANEELELRVAKRTQDLEKSRQ
KMQEQHEELVRTYFDLEEETAQRLNALEELARKERMLLQQNRLAALGEMINNIAHQWRQPLNELGLIVQELPIMYDRGDF
NKEYLRESVAKFMKVLSHTSKTIDDFRTFFKPDREMVPFRVTEVVDKALSLVAESLKHLEIKVTVHSVDDPAIMGHPNEF
SQAILNILFNARDAFKERSISCRQIEIRIFLEDETAVVTIGDNAGGIPEEIMDKIFDPYFTTRGPEQGTGIGLYMTKMII
EKNIPGKLSVRNTEKGAEFRIEVGKVASRQH

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. DctB functions as a membrane-associated protein kinase that phosphorylates dctD in response to environmental signals [H]

COG id: COG0642

COG function: function code T; Signal transduction histidine kinase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=275, Percent_Identity=25.8181818181818, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1790436, Length=238, Percent_Identity=25.2100840336134, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR017055
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 85757; Mature: 85757

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSRLRDMPLYNSRIINSYILLIKHKYSHVDISELLLYAGMKEYEVADQAHWFSQEQID
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHH
RFHEKLQQMTGNARISREAGRYAASPDVLGAMRQYILGLVDASSTFDIISKTTAKFTRSS
HHHHHHHHHHCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SYQSRSIASNQVEITVTPYEPGLEKPYQCENRIGFFEAIVLVFNHKMTDLQYFPEIRNLP
CCCHHCCCCCEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
TIEHPECMFRGDPVCRYIVTWEKTLFTFLKKMRNLLALPLAALNLALLAMGDTGVLTWVF
CCCCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHH
PSSLCVLLLVALATESSEKKAIKESLWSTRDSIENLLDQINLNYNNAMLTHEIGQRLGNY
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCH
TRIEEMLFDVAQIMRYRLEYDRGMIFLADGGRKRLELRASYGYKDEELACLNSLPFLLEN
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCHHHCC
RELGDVYLECFRGQRPFLVNELSSGSVGENTLACAHMSGTRAFICCPIVADGASLGVLTV
CCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCEEEE
ENVQVKRPLVERDISLIMGTASVLGISIRNCELIGALETANEELELRVAKRTQDLEKSRQ
CCCCHHCCHHHHHHHHHHHHHHHHEEEECCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
KMQEQHEELVRTYFDLEEETAQRLNALEELARKERMLLQQNRLAALGEMINNIAHQWRQP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNELGLIVQELPIMYDRGDFNKEYLRESVAKFMKVLSHTSKTIDDFRTFFKPDREMVPFR
HHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VTEVVDKALSLVAESLKHLEIKVTVHSVDDPAIMGHPNEFSQAILNILFNARDAFKERSI
HHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCC
SCRQIEIRIFLEDETAVVTIGDNAGGIPEEIMDKIFDPYFTTRGPEQGTGIGLYMTKMII
CEEEEEEEEEEECCEEEEEECCCCCCCHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHH
EKNIPGKLSVRNTEKGAEFRIEVGKVASRQH
HCCCCCCEEECCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure
MQPSRLRDMPLYNSRIINSYILLIKHKYSHVDISELLLYAGMKEYEVADQAHWFSQEQID
CCCCCCCCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHCCCHHHHHHHHHHHCCHHHHH
RFHEKLQQMTGNARISREAGRYAASPDVLGAMRQYILGLVDASSTFDIISKTTAKFTRSS
HHHHHHHHHHCCCEEEHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
SYQSRSIASNQVEITVTPYEPGLEKPYQCENRIGFFEAIVLVFNHKMTDLQYFPEIRNLP
CCCHHCCCCCEEEEEEECCCCCCCCCCCHHHCHHHHHHHHHHHCCCHHHHHHHHHHCCCC
TIEHPECMFRGDPVCRYIVTWEKTLFTFLKKMRNLLALPLAALNLALLAMGDTGVLTWVF
CCCCCCHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEHH
PSSLCVLLLVALATESSEKKAIKESLWSTRDSIENLLDQINLNYNNAMLTHEIGQRLGNY
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCH
TRIEEMLFDVAQIMRYRLEYDRGMIFLADGGRKRLELRASYGYKDEELACLNSLPFLLEN
HHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEECCCCCCHHHHHHHCCCHHHCC
RELGDVYLECFRGQRPFLVNELSSGSVGENTLACAHMSGTRAFICCPIVADGASLGVLTV
CCHHHHHHHHHCCCCCCEEECCCCCCCCCCCEEEEEECCCCEEEEEEEEECCCCCCEEEE
ENVQVKRPLVERDISLIMGTASVLGISIRNCELIGALETANEELELRVAKRTQDLEKSRQ
CCCCHHCCHHHHHHHHHHHHHHHHEEEECCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHH
KMQEQHEELVRTYFDLEEETAQRLNALEELARKERMLLQQNRLAALGEMINNIAHQWRQP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LNELGLIVQELPIMYDRGDFNKEYLRESVAKFMKVLSHTSKTIDDFRTFFKPDREMVPFR
HHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
VTEVVDKALSLVAESLKHLEIKVTVHSVDDPAIMGHPNEFSQAILNILFNARDAFKERSI
HHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCEECCCHHHHHHHHHHHHHHHHHHHHCCC
SCRQIEIRIFLEDETAVVTIGDNAGGIPEEIMDKIFDPYFTTRGPEQGTGIGLYMTKMII
CEEEEEEEEEEECCEEEEEECCCCCCCHHHHHHHHCCCHHCCCCCCCCCCHHHHHHHHHH
EKNIPGKLSVRNTEKGAEFRIEVGKVASRQH
HCCCCCCEEECCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3671068 [H]