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The map label for this gene is lcfB [H]

Identifier: 197118482

GI number: 197118482

Start: 2424829

End: 2426469

Strand: Reverse

Name: lcfB [H]

Synonym: Gbem_2100

Alternate gene names: 197118482

Gene position: 2426469-2424829 (Counterclockwise)

Preceding gene: 197118483

Following gene: 197118481

Centisome position: 52.58

GC content: 64.59

Gene sequence:

>1641_bases
ATGCTAGAGAGCGAACTGGTCAACATTGCAGCGCACCTGCCGGAGATGGCGAAGCGCCAGCCCGACACGAGGGCCATCAT
CTTCCCCAAGCAGAACCGGAGCTTGAGCTTCTCCGAGCTCAACACGCTGAGCGACCGGATCGCACGGGGGCTGATCGCCA
ACGGCATCTGCCGCGGCGTGCGCACTGTGCTTATGGTGACGCCTAGCCCGGAGTTCTTCGCTCTTACCTTCGCGCTCTTC
AAGGTGGGCGCGGTGCCGGTATTGATCGACCCGGGGCTGGGGATCAAGAACCTGAAGCAGTGCTTTGCCGAGGCGCAGCC
GCACGCCTTCATCGGCATCCCCAAGGCGCACCTAGCGCGGCTGATCTTCGGCTGGGGCAAGGAGACCATCCGGACCTGGA
TCACCGTCGGTCCGCGCCTTTTCTGGGAAGGAACCACCCTCAACAGGATCATCGACGAGCACACCGACGCTTCCCCCTTC
GTCCCCGCCCCGACCAGCTCCGATGACGTCGCCGCCATCCTCTTCACCAGCGGCAGCACTGGGGTTCCCAAGGGGGCGGT
CTACAGCCACGGCAATTTCGCGGCGCAGGTGCAGGCGCTGAAGCAGGTCTACGGCATCGAGCCGGGCGAGATCGACCTCC
CTACCTTCCCGCTCTTCGCCCTTTTCGCCCCCGCCCTCGGCATGACCGCCGTTATCCCGGAGATGGACTTTACCCGCCCG
GGGTCGGTGGACCCGAAGAAGATCATCGGCGCCATCCATACCTACGGCGTCACCACCATGTTCGGCTCACCCGCACTGAT
TAACCGGGTCGGGCGCTACGGGGTGCAGCACCAGGTGAAGCTTCCCACCTTGAGACGCGCCATCTCGGCCGGCGCCCCGG
TGTCGGCTGCGGTACTGGAGCGCTTCACTTCGCTCCTGAACCCCGGCGTGCAGGTCTTCACCCCTTACGGCGCCACCGAG
GCGCTCCCCGTCTGCTCCATCGGCAGCACCGAGATCCTGGAAACCACCCGGAAGATCACCGATGCCGGCGGCGGCGTCTG
CGTCGGCAGGCCGGTCGAGGGGATCCGACTGGAGATCGTCCAGATCAGCGACGACCCTATCTGCAGCTGGCACGAATCGC
TGCGGGTACCGACCGGGAAGATCGGCGAGATCGTGGTGCAGGGGGAGCAGGTCACCAGGGGGTACTACAACCGCCCGGAA
TCGGACCATCTCTCCAAGATCGCCGATCCCGAGACAGGTTCCTTCTTCCACCGCATGGGGGATCTGGGCGGGCGCGACGA
GGAAGGTAGGATCTGGTTCTGCGGACGCAAGTCGCACCGCGTGGAGACCGAGTCCGGGCCGCTTTACACTATCCCGTGCG
AGGCAGTGTTCAACGCCCACCCGGCGGTGTTCCGCACCGCGCTGGTGGGGGTGGGAGCACCCGGCGAGCTGAAGCCCGTG
CTCTGCGTGGAGCTGGAAAAGGACGTCAAGGTGGATCCTGAACTGGTCCGGGCCGAGCTTCTCTCATACGCACAGGACCA
CATCCACACCAAGTCGATTGAAACCATACTATTCCACCCGGCCTTCCCGGTGGACATCCGTCACAACGCCAAGATCTTTC
GGGAAAAACTGGCGGTCTGGGCGGCTGCGAGGCTCAAATGA

Upstream 100 bases:

>100_bases
GACGCCGGACACTACATCCTGGAAGACATGAAGCAAGAAGTCGTGCCGATGATTGCCGAATTCCTGCGAGAGACTAAACC
CGGGGAGATCCGTTAAGGCC

Downstream 100 bases:

>100_bases
AGGCTCTCGTGACCGGCGGCGGGGGCTTCTTAGGCTCGGCCATTGTGCGCCAGTTGCTGGCCCGGGGGGACCAGGCGGTC
AGCTTCTCGCGCGGCGAGTA

Product: peptide synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MLESELVNIAAHLPEMAKRQPDTRAIIFPKQNRSLSFSELNTLSDRIARGLIANGICRGVRTVLMVTPSPEFFALTFALF
KVGAVPVLIDPGLGIKNLKQCFAEAQPHAFIGIPKAHLARLIFGWGKETIRTWITVGPRLFWEGTTLNRIIDEHTDASPF
VPAPTSSDDVAAILFTSGSTGVPKGAVYSHGNFAAQVQALKQVYGIEPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRP
GSVDPKKIIGAIHTYGVTTMFGSPALINRVGRYGVQHQVKLPTLRRAISAGAPVSAAVLERFTSLLNPGVQVFTPYGATE
ALPVCSIGSTEILETTRKITDAGGGVCVGRPVEGIRLEIVQISDDPICSWHESLRVPTGKIGEIVVQGEQVTRGYYNRPE
SDHLSKIADPETGSFFHRMGDLGGRDEEGRIWFCGRKSHRVETESGPLYTIPCEAVFNAHPAVFRTALVGVGAPGELKPV
LCVELEKDVKVDPELVRAELLSYAQDHIHTKSIETILFHPAFPVDIRHNAKIFREKLAVWAAARLK

Sequences:

>Translated_546_residues
MLESELVNIAAHLPEMAKRQPDTRAIIFPKQNRSLSFSELNTLSDRIARGLIANGICRGVRTVLMVTPSPEFFALTFALF
KVGAVPVLIDPGLGIKNLKQCFAEAQPHAFIGIPKAHLARLIFGWGKETIRTWITVGPRLFWEGTTLNRIIDEHTDASPF
VPAPTSSDDVAAILFTSGSTGVPKGAVYSHGNFAAQVQALKQVYGIEPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRP
GSVDPKKIIGAIHTYGVTTMFGSPALINRVGRYGVQHQVKLPTLRRAISAGAPVSAAVLERFTSLLNPGVQVFTPYGATE
ALPVCSIGSTEILETTRKITDAGGGVCVGRPVEGIRLEIVQISDDPICSWHESLRVPTGKIGEIVVQGEQVTRGYYNRPE
SDHLSKIADPETGSFFHRMGDLGGRDEEGRIWFCGRKSHRVETESGPLYTIPCEAVFNAHPAVFRTALVGVGAPGELKPV
LCVELEKDVKVDPELVRAELLSYAQDHIHTKSIETILFHPAFPVDIRHNAKIFREKLAVWAAARLK
>Mature_546_residues
MLESELVNIAAHLPEMAKRQPDTRAIIFPKQNRSLSFSELNTLSDRIARGLIANGICRGVRTVLMVTPSPEFFALTFALF
KVGAVPVLIDPGLGIKNLKQCFAEAQPHAFIGIPKAHLARLIFGWGKETIRTWITVGPRLFWEGTTLNRIIDEHTDASPF
VPAPTSSDDVAAILFTSGSTGVPKGAVYSHGNFAAQVQALKQVYGIEPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRP
GSVDPKKIIGAIHTYGVTTMFGSPALINRVGRYGVQHQVKLPTLRRAISAGAPVSAAVLERFTSLLNPGVQVFTPYGATE
ALPVCSIGSTEILETTRKITDAGGGVCVGRPVEGIRLEIVQISDDPICSWHESLRVPTGKIGEIVVQGEQVTRGYYNRPE
SDHLSKIADPETGSFFHRMGDLGGRDEEGRIWFCGRKSHRVETESGPLYTIPCEAVFNAHPAVFRTALVGVGAPGELKPV
LCVELEKDVKVDPELVRAELLSYAQDHIHTKSIETILFHPAFPVDIRHNAKIFREKLAVWAAARLK

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI195947378, Length=416, Percent_Identity=21.6346153846154, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI38524616, Length=503, Percent_Identity=23.2604373757455, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788107, Length=380, Percent_Identity=27.6315789473684, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI145693145, Length=478, Percent_Identity=23.6401673640167, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1789201, Length=349, Percent_Identity=22.3495702005731, Blast_Score=63, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI71994703, Length=551, Percent_Identity=23.5934664246824, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994694, Length=551, Percent_Identity=23.5934664246824, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71994690, Length=551, Percent_Identity=23.5934664246824, Blast_Score=97, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=512, Percent_Identity=23.2421875, Blast_Score=96, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=343, Percent_Identity=25.3644314868805, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=345, Percent_Identity=25.2173913043478, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560308, Length=500, Percent_Identity=23.2, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581924, Length=367, Percent_Identity=25.8855585831063, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI20130357, Length=422, Percent_Identity=23.2227488151659, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 59455; Mature: 59455

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESELVNIAAHLPEMAKRQPDTRAIIFPKQNRSLSFSELNTLSDRIARGLIANGICRGV
CCHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RTVLMVTPSPEFFALTFALFKVGAVPVLIDPGLGIKNLKQCFAEAQPHAFIGIPKAHLAR
EEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHH
LIFGWGKETIRTWITVGPRLFWEGTTLNRIIDEHTDASPFVPAPTSSDDVAAILFTSGST
HHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
GVPKGAVYSHGNFAAQVQALKQVYGIEPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRP
CCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
GSVDPKKIIGAIHTYGVTTMFGSPALINRVGRYGVQHQVKLPTLRRAISAGAPVSAAVLE
CCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHCCCCEEECHHHHHHHHCCCCHHHHHHH
RFTSLLNPGVQVFTPYGATEALPVCSIGSTEILETTRKITDAGGGVCVGRPVEGIRLEIV
HHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEEE
QISDDPICSWHESLRVPTGKIGEIVVQGEQVTRGYYNRPESDHLSKIADPETGSFFHRMG
EECCCCCCCHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHH
DLGGRDEEGRIWFCGRKSHRVETESGPLYTIPCEAVFNAHPAVFRTALVGVGAPGELKPV
CCCCCCCCCCEEEECCCCCCEECCCCCEEEECHHHHHCCCHHHHHHHHHCCCCCCCCCCE
LCVELEKDVKVDPELVRAELLSYAQDHIHTKSIETILFHPAFPVDIRHNAKIFREKLAVW
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHH
AAARLK
HHHHCC
>Mature Secondary Structure
MLESELVNIAAHLPEMAKRQPDTRAIIFPKQNRSLSFSELNTLSDRIARGLIANGICRGV
CCHHHHHHHHHHCHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
RTVLMVTPSPEFFALTFALFKVGAVPVLIDPGLGIKNLKQCFAEAQPHAFIGIPKAHLAR
EEEEEECCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCEEEECCHHHHHH
LIFGWGKETIRTWITVGPRLFWEGTTLNRIIDEHTDASPFVPAPTSSDDVAAILFTSGST
HHHHCCHHHHHHHHHCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
GVPKGAVYSHGNFAAQVQALKQVYGIEPGEIDLPTFPLFALFAPALGMTAVIPEMDFTRP
CCCCCCEECCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCEEECCCCCCCCC
GSVDPKKIIGAIHTYGVTTMFGSPALINRVGRYGVQHQVKLPTLRRAISAGAPVSAAVLE
CCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHCCCCEEECHHHHHHHHCCCCHHHHHHH
RFTSLLNPGVQVFTPYGATEALPVCSIGSTEILETTRKITDAGGGVCVGRPVEGIRLEIV
HHHHHHCCCCEEEECCCCCCCCEEECCCCHHHHHHHHHHHCCCCCEEECCCCCCEEEEEE
QISDDPICSWHESLRVPTGKIGEIVVQGEQVTRGYYNRPESDHLSKIADPETGSFFHRMG
EECCCCCCCHHHHCCCCCCCCCEEEEECCHHHHCCCCCCCHHHHHHHCCCCCCHHHHHHH
DLGGRDEEGRIWFCGRKSHRVETESGPLYTIPCEAVFNAHPAVFRTALVGVGAPGELKPV
CCCCCCCCCCEEEECCCCCCEECCCCCEEEECHHHHHCCCHHHHHHHHHCCCCCCCCCCE
LCVELEKDVKVDPELVRAELLSYAQDHIHTKSIETILFHPAFPVDIRHNAKIFREKLAVW
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHH
AAARLK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]