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The map label for this gene is ybjS [C]

Identifier: 197118481

GI number: 197118481

Start: 2423840

End: 2424832

Strand: Reverse

Name: ybjS [C]

Synonym: Gbem_2099

Alternate gene names: 197118481

Gene position: 2424832-2423840 (Counterclockwise)

Preceding gene: 197118482

Following gene: 197118480

Centisome position: 52.54

GC content: 68.18

Gene sequence:

>993_bases
ATGAAGGCTCTCGTGACCGGCGGCGGGGGCTTCTTAGGCTCGGCCATTGTGCGCCAGTTGCTGGCCCGGGGGGACCAGGC
GGTCAGCTTCTCGCGCGGCGAGTACCCGGAACTGGCCCTGCTGGGCGTGGAACAGCGCCGGGGCGACCTGTCGGATCCGG
AAGCGGTGGCGGACGCGGCCCGGGGGTGCGACGTCGTTTTCCATGTCGCCGCGAAGGCGGGGATCTGGGGGGAGTTCGAG
GAGTACTACCGCGCCAACGTCACCGGCACCGAAAACGTCATCGAGGCCTGCCGCAGGCTCGGCATCGAAAGGCTCGTCTA
TACCAGCTCCCCAAGCGTCGTCTTCGACGGATCCGACGTGGAGGGAGGGGACGAGTCGCTCCCCTACCCTGCGCATTTCG
AGGCGCATTACCCACACACGAAGGCACTGGCCGAACAGGCGGTGCTCGCGGCCAATGCACCTGAGCTGGCGACCGTGTCG
CTGCGCCCCCACCTGATCTGGGGCCCCGGCGACAACCACCTGGTGCCGCGCATCGTGGCGAAGGCGCGATCGGGCGCGTT
GAAGCGGATCGGCAACCGCCCCTGCCTGGTGGACACGGTGTACGTCGAGAACGCCGCCGAGGCGCACCTGAACGCGGCTG
ACCGGCTGAAAGCCGGAAGCGCGCCGGCGGGAAAGGCGTATTTCATCTCCAACGGCGAACCCATCCCGCTCTGGGAGATG
GTGAACCGGATCCTCGCGGCGGCAGGGCTTCCCCCGATAACGCGCCAGGTCTCCCCGGGCCTTGCCTACGGCGCCGGCGT
GGTCTGCGAGACCCTCTGGAAGATGCTGCGCCTCTCCGGCGAGCCCCCCATGACCCGCTTCGTAGCCAAGGAACTGGCCA
CGGCGCACTGGTTCGACCTCTCCGCTGCGCGCGCCGATCTCGGTTACCACCCCCGCATATCCATCGACGAAGGGCTTAGG
CTGCTGCAAGACGCCCTGAGGCAAGGTCGTTGA

Upstream 100 bases:

>100_bases
TTGAAACCATACTATTCCACCCGGCCTTCCCGGTGGACATCCGTCACAACGCCAAGATCTTTCGGGAAAAACTGGCGGTC
TGGGCGGCTGCGAGGCTCAA

Downstream 100 bases:

>100_bases
TACCGGTTCCGGGCGAGGAAGGAGTCTTCTTCGAGCTGGCTTCCCTGGACCGCTCCGAAGCGGAAAGAAAGCGTCTGTTC
GCCCACCTCTCGCCGGATGA

Product: 3-beta-hydroxysteroid dehydrogenase/isomerase family protein

Products: NA

Alternate protein names: Cholesterol dehydrogenase; 3-beta-hydroxy-Delta(5)-steroid dehydrogenase; 3-beta-HSD; 3BHSD; 3-beta hydroxysterol dehydrogenase; 3-beta-hydroxy-5-ene steroid dehydrogenase; Progesterone reductase; Steroid Delta-isomerase; Delta-5-3-ketosteroid isomerase [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKALVTGGGGFLGSAIVRQLLARGDQAVSFSRGEYPELALLGVEQRRGDLSDPEAVADAARGCDVVFHVAAKAGIWGEFE
EYYRANVTGTENVIEACRRLGIERLVYTSSPSVVFDGSDVEGGDESLPYPAHFEAHYPHTKALAEQAVLAANAPELATVS
LRPHLIWGPGDNHLVPRIVAKARSGALKRIGNRPCLVDTVYVENAAEAHLNAADRLKAGSAPAGKAYFISNGEPIPLWEM
VNRILAAAGLPPITRQVSPGLAYGAGVVCETLWKMLRLSGEPPMTRFVAKELATAHWFDLSAARADLGYHPRISIDEGLR
LLQDALRQGR

Sequences:

>Translated_330_residues
MKALVTGGGGFLGSAIVRQLLARGDQAVSFSRGEYPELALLGVEQRRGDLSDPEAVADAARGCDVVFHVAAKAGIWGEFE
EYYRANVTGTENVIEACRRLGIERLVYTSSPSVVFDGSDVEGGDESLPYPAHFEAHYPHTKALAEQAVLAANAPELATVS
LRPHLIWGPGDNHLVPRIVAKARSGALKRIGNRPCLVDTVYVENAAEAHLNAADRLKAGSAPAGKAYFISNGEPIPLWEM
VNRILAAAGLPPITRQVSPGLAYGAGVVCETLWKMLRLSGEPPMTRFVAKELATAHWFDLSAARADLGYHPRISIDEGLR
LLQDALRQGR
>Mature_330_residues
MKALVTGGGGFLGSAIVRQLLARGDQAVSFSRGEYPELALLGVEQRRGDLSDPEAVADAARGCDVVFHVAAKAGIWGEFE
EYYRANVTGTENVIEACRRLGIERLVYTSSPSVVFDGSDVEGGDESLPYPAHFEAHYPHTKALAEQAVLAANAPELATVS
LRPHLIWGPGDNHLVPRIVAKARSGALKRIGNRPCLVDTVYVENAAEAHLNAADRLKAGSAPAGKAYFISNGEPIPLWEM
VNRILAAAGLPPITRQVSPGLAYGAGVVCETLWKMLRLSGEPPMTRFVAKELATAHWFDLSAARADLGYHPRISIDEGLR
LLQDALRQGR

Specific function: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidation and isomerization of cholesterol, pregnenolone, and dehydroepiandrosterone (DHEA) into cholest-4-en-3-one, progesterone, and androsterone, respectively [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-beta-HSD family [H]

Homologues:

Organism=Homo sapiens, GI193211614, Length=339, Percent_Identity=35.693215339233, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI8393516, Length=339, Percent_Identity=35.693215339233, Blast_Score=198, Evalue=6e-51,
Organism=Homo sapiens, GI116268111, Length=328, Percent_Identity=34.4512195121951, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI310132178, Length=334, Percent_Identity=34.7305389221557, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI310113012, Length=334, Percent_Identity=34.7305389221557, Blast_Score=152, Evalue=5e-37,
Organism=Homo sapiens, GI239745448, Length=334, Percent_Identity=34.7305389221557, Blast_Score=151, Evalue=6e-37,
Organism=Homo sapiens, GI4504507, Length=345, Percent_Identity=31.8840579710145, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI260763931, Length=345, Percent_Identity=31.5942028985507, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI4504509, Length=345, Percent_Identity=31.5942028985507, Blast_Score=113, Evalue=2e-25,
Organism=Homo sapiens, GI19923621, Length=350, Percent_Identity=33.7142857142857, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI218563686, Length=165, Percent_Identity=41.8181818181818, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI218563684, Length=165, Percent_Identity=41.8181818181818, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI87081792, Length=330, Percent_Identity=28.4848484848485, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1786974, Length=294, Percent_Identity=26.8707482993197, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI193211272, Length=338, Percent_Identity=27.5147928994083, Blast_Score=108, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17570557, Length=336, Percent_Identity=26.4880952380952, Blast_Score=102, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17509709, Length=361, Percent_Identity=25.4847645429363, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6321437, Length=340, Percent_Identity=35, Blast_Score=168, Evalue=1e-42,
Organism=Drosophila melanogaster, GI21355349, Length=282, Percent_Identity=29.7872340425532, Blast_Score=76, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =1.1.1.145; =5.3.3.1 [H]

Molecular weight: Translated: 35466; Mature: 35466

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKALVTGGGGFLGSAIVRQLLARGDQAVSFSRGEYPELALLGVEQRRGDLSDPEAVADAA
CCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECHHHCCCCCCHHHHHHHH
RGCDVVFHVAAKAGIWGEFEEYYRANVTGTENVIEACRRLGIERLVYTSSPSVVFDGSDV
CCCHHEEHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCCCEEECCCCC
EGGDESLPYPAHFEAHYPHTKALAEQAVLAANAPELATVSLRPHLIWGPGDNHLVPRIVA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHH
KARSGALKRIGNRPCLVDTVYVENAAEAHLNAADRLKAGSAPAGKAYFISNGEPIPLWEM
HHHHHHHHHHCCCCCEEEEEECCCHHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCHHHH
VNRILAAAGLPPITRQVSPGLAYGAGVVCETLWKMLRLSGEPPMTRFVAKELATAHWFDL
HHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SAARADLGYHPRISIDEGLRLLQDALRQGR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKALVTGGGGFLGSAIVRQLLARGDQAVSFSRGEYPELALLGVEQRRGDLSDPEAVADAA
CCEEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECHHHCCCCCCHHHHHHHH
RGCDVVFHVAAKAGIWGEFEEYYRANVTGTENVIEACRRLGIERLVYTSSPSVVFDGSDV
CCCHHEEHHHHHCCCCHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEECCCCEEECCCCC
EGGDESLPYPAHFEAHYPHTKALAEQAVLAANAPELATVSLRPHLIWGPGDNHLVPRIVA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCHHHHHHH
KARSGALKRIGNRPCLVDTVYVENAAEAHLNAADRLKAGSAPAGKAYFISNGEPIPLWEM
HHHHHHHHHHCCCCCEEEEEECCCHHHHHCCHHHHHCCCCCCCCCEEEECCCCCCCHHHH
VNRILAAAGLPPITRQVSPGLAYGAGVVCETLWKMLRLSGEPPMTRFVAKELATAHWFDL
HHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
SAARADLGYHPRISIDEGLRLLQDALRQGR
HHHHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]