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The map label for this gene is bioD [H]

Identifier: 197118434

GI number: 197118434

Start: 2366122

End: 2366841

Strand: Reverse

Name: bioD [H]

Synonym: Gbem_2052

Alternate gene names: 197118434

Gene position: 2366841-2366122 (Counterclockwise)

Preceding gene: 197118435

Following gene: 197118433

Centisome position: 51.28

GC content: 63.19

Gene sequence:

>720_bases
ATGGCTGCAAAAAGCATTTTCATAACCGGCACCGACACCGGGGTCGGCAAGACCATCGCATCGGCCACCATCGCCATGCT
GCTCAGAAGAATGGGTCATAAGGTCGCAGTGATGAAGCCCGTGACCAGCGGCTGTATCGAGCGCGACGGGCGTCTGGTCT
CGGAGGACGCAGAGCTTCTGTGCTACGCCGCGGGCGTCCCCGTTACTCCGGACTCCGCCCCCTATCTCTTGAGGGCTCCG
GTTGCTCCCTCCATCGCCGCCTCGCAGGACGGGGTCCGGATCAGCTTCGACACCATCAAGGAGGCGTACCAAAGGCTCGC
CGAAAGCAACGACTTCGTCATCGTCGAGGGTGCCGGGGGACTGATGGTCCCTCTTGCCGGCGGGCTACTGGTAGCCGACC
TGGCCCAGCACCTGGGACTTCCCATCGCCGTGGTGGCAAGGCCGGACCTGGGAACCGTCAACCATACGCTTCTCACCGTC
TTCTGCGCCCGCACCATGGGGCTCAAGGTCAAAGGGGTCATCGTCAATCGCTACCCCGATTCCCCGGGGGAAGCCGAGTC
CTATGCGCCGCACATGATAGATTCCCTCTCCGGCGCGCAGCTGCTCGGGCTTTTCCCCGATACCGACGGGAAAGACGACC
GCGAAGTGACGGAGAGGCTCGTCGAGCGCTTGCTGCAACAACCGGCGACGGGGATCATGCTGCGGGAGCTGTTCGATTAA

Upstream 100 bases:

>100_bases
GTCGACACCGACCTCACCATGTTCAGCGACCTGGGGCTTAAGACCGTCATGTGCGCCCACTGAGCCGCCCTCAAATACCG
CCACGTAAGGAAAGATAACC

Downstream 100 bases:

>100_bases
CAGCAATTGACAATTGACGATTGATAATTGACAACGAAAAACCCTGAACACAGCAGGGACTGACGTTGAGGAGATTTGAA
CGTGGTTGAACTGGATACGG

Product: dethiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase [H]

Number of amino acids: Translated: 239; Mature: 238

Protein sequence:

>239_residues
MAAKSIFITGTDTGVGKTIASATIAMLLRRMGHKVAVMKPVTSGCIERDGRLVSEDAELLCYAAGVPVTPDSAPYLLRAP
VAPSIAASQDGVRISFDTIKEAYQRLAESNDFVIVEGAGGLMVPLAGGLLVADLAQHLGLPIAVVARPDLGTVNHTLLTV
FCARTMGLKVKGVIVNRYPDSPGEAESYAPHMIDSLSGAQLLGLFPDTDGKDDREVTERLVERLLQQPATGIMLRELFD

Sequences:

>Translated_239_residues
MAAKSIFITGTDTGVGKTIASATIAMLLRRMGHKVAVMKPVTSGCIERDGRLVSEDAELLCYAAGVPVTPDSAPYLLRAP
VAPSIAASQDGVRISFDTIKEAYQRLAESNDFVIVEGAGGLMVPLAGGLLVADLAQHLGLPIAVVARPDLGTVNHTLLTV
FCARTMGLKVKGVIVNRYPDSPGEAESYAPHMIDSLSGAQLLGLFPDTDGKDDREVTERLVERLLQQPATGIMLRELFD
>Mature_238_residues
AAKSIFITGTDTGVGKTIASATIAMLLRRMGHKVAVMKPVTSGCIERDGRLVSEDAELLCYAAGVPVTPDSAPYLLRAPV
APSIAASQDGVRISFDTIKEAYQRLAESNDFVIVEGAGGLMVPLAGGLLVADLAQHLGLPIAVVARPDLGTVNHTLLTVF
CARTMGLKVKGVIVNRYPDSPGEAESYAPHMIDSLSGAQLLGLFPDTDGKDDREVTERLVERLLQQPATGIMLRELFD

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1786995, Length=207, Percent_Identity=32.3671497584541, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI87081944, Length=222, Percent_Identity=31.981981981982, Blast_Score=76, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=188, Percent_Identity=35.1063829787234, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004472
- InterPro:   IPR002586 [H]

Pfam domain/function: PF01656 CbiA [H]

EC number: =6.3.3.3 [H]

Molecular weight: Translated: 25342; Mature: 25210

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKSIFITGTDTGVGKTIASATIAMLLRRMGHKVAVMKPVTSGCIERDGRLVSEDAELL
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCEEECCCCEEE
CYAAGVPVTPDSAPYLLRAPVAPSIAASQDGVRISFDTIKEAYQRLAESNDFVIVEGAGG
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCC
LMVPLAGGLLVADLAQHLGLPIAVVARPDLGTVNHTLLTVFCARTMGLKVKGVIVNRYPD
EEEHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCC
SPGEAESYAPHMIDSLSGAQLLGLFPDTDGKDDREVTERLVERLLQQPATGIMLRELFD
CCCCCHHCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCC
>Mature Secondary Structure 
AAKSIFITGTDTGVGKTIASATIAMLLRRMGHKVAVMKPVTSGCIERDGRLVSEDAELL
CCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHCCCEEECCCCEEE
CYAAGVPVTPDSAPYLLRAPVAPSIAASQDGVRISFDTIKEAYQRLAESNDFVIVEGAGG
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCEEEEHHHHHHHHHHHHCCCCEEEEECCCC
LMVPLAGGLLVADLAQHLGLPIAVVARPDLGTVNHTLLTVFCARTMGLKVKGVIVNRYPD
EEEHHCCHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHCEEEEEEEEECCCC
SPGEAESYAPHMIDSLSGAQLLGLFPDTDGKDDREVTERLVERLLQQPATGIMLRELFD
CCCCCHHCCCHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA