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The map label for this gene is alkK [H]

Identifier: 197118402

GI number: 197118402

Start: 2324679

End: 2326325

Strand: Direct

Name: alkK [H]

Synonym: Gbem_2020

Alternate gene names: 197118402

Gene position: 2324679-2326325 (Clockwise)

Preceding gene: 197118401

Following gene: 308535281

Centisome position: 50.37

GC content: 61.32

Gene sequence:

>1647_bases
ATGAGCGATTTGCTGATCCCCCGTACCGCGTCCGCCTACAATTACCCTTTGCTGATAAAGAACCTGTTGCTCTACCCGGT
GGTCGACAACCCCGACCAGGAGATCGTATACCGTGACCTGTACCGGGGCAATTACCGCCAACTGAGGGAGCGGGTGAAGC
GGCTGGCCAACATGCTCACCGGGCTCGGCGTGAAGCCGGGGCAAACGGTGGCCGTGATGGACTGGGACAGCCATCGCTAC
CTGGAACTTTTCTTCGCGGTGCCGATGATCGGCGCGGTGCTCCACACCATAAACGTGCGCCTGTCCGCGGAGCAGATCCT
TTACACCATCGACCATGCCGAGGACGACGTGCTGCTGGTCAACAGCGAGTTTCTCCCCATCATGGAGCAGATCCGCGGCA
GGATCGACAACGTCCGCACCTATATCCTCATCTCCGATGACGGCATGACGGAATGCAGCACCATCCCCGCCTGCGGCGAG
TACGAACAGCTCCTGGCCCAGGCTTCGCCGGAGTTCGAGTTCCCCGACCTGGACGAGAACACCAGGGCCACGACTTTCTA
CACCACCGGAACCACGGGGATGCCGAAGGGGGTCTATTTCAGCCACCGGCAACTGGTGCTTCATTCGCTGGGACTTTTGG
CGACGCTCGGTTCCTCCACCTCGCACGCCTGTCTGCACCGCGACGACGTCTACATGCCGATTACCCCGATGTTCCACGTT
CATGCCTGGGGGGTCCCCTATATCGCCACCATGCTGGGGGTGAAGCAGGTCTATCCCGGGCGCTACCTGCCGGAAACCCT
GCTGGAGCTCAAGGAGAAGGAAGGGGTCAGTTTCTCCCATTGCGTTCCGACCATCCTGCACATGCTCTTGAAGCACCCCC
TTGCGGAAAAGATCGACCTGCGGGGGTGGAAACTCATCATCGGCGGCGCGGCTTTGTCGCGCAACCTCTGCGTCGAGTCG
CTGAGGCTTGGCATCGACGTCTTCACCGGGTACGGGATGTCCGAGACCTGCCCGATCCTCACCATTTCCAAGCTCACACC
GGAGATGCTGGAACTATCCCACGCCGAGCAGGCGGAAATCCGCTGCAAAACGGGTCTTGCCCTGGCCTTAGTCGATCTGC
GCGTGGTCGACAGCAACTTCAACGAGCTCCCCCGCGACGGCGTCAGCGCCGGCAACGTGGTGGTGCGCTCCCCCTGGCTT
TCGCAGGGATACCTGAAGGATCACAAGGCCTCCGAGCGTCTCTGGGAGGGGGGGTATCTCCATACCGGCGACGTGGCGGT
GCGGGACGAGCTGGGCTATCTCAAGATCACCGACCGGAGCAAGGACGTGATCAAGGTCGCCGGCGAATGGGTTTCCTCGC
TGGAGCTGGAGGACATTGTCGCGCATCACCCGGCAGTAGCCGAGGTGGCGGTGATAGGGAAGCCTGACGAGAAGTGGGGC
GAGCGCCCCCTGGCGCTGGTCGTTCTCAAGCCGACCGAGGGGACGAAGGTGACCGATAAGGAGATCGCCCACCACGTGAG
GGAGTACGCCGACAAGGGTGTGGTGAGCAAGCAGGTCGTCCTGGTCAAGGTGAAGCTCGTTCCATCCATCGACAAGACTA
GCGTTGGAAAGATCAACAAGGTGGCACTGCGGGAGAAATATCTCTGA

Upstream 100 bases:

>100_bases
ATTGGACGAAACAGGTCCCCCCTCCCAGGCGGGAGGGGGGGAGCAGACCTGCACTTTTCCGGCGGCATAGCCGGTTTTAT
TTGAAACAGGAGACCCATTG

Downstream 100 bases:

>100_bases
GAGTGAGACAGGGTGGAAAATGAAGGGAGCTAAAAAGAAGGAGTTCAGCATGAACGCGCTGGAGATTGAGGTGGCCATCG
CCGCGCTTCATTCCACAGGG

Product: long-chain-fatty-acid--CoA ligase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSDLLIPRTASAYNYPLLIKNLLLYPVVDNPDQEIVYRDLYRGNYRQLRERVKRLANMLTGLGVKPGQTVAVMDWDSHRY
LELFFAVPMIGAVLHTINVRLSAEQILYTIDHAEDDVLLVNSEFLPIMEQIRGRIDNVRTYILISDDGMTECSTIPACGE
YEQLLAQASPEFEFPDLDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSLGLLATLGSSTSHACLHRDDVYMPITPMFHV
HAWGVPYIATMLGVKQVYPGRYLPETLLELKEKEGVSFSHCVPTILHMLLKHPLAEKIDLRGWKLIIGGAALSRNLCVES
LRLGIDVFTGYGMSETCPILTISKLTPEMLELSHAEQAEIRCKTGLALALVDLRVVDSNFNELPRDGVSAGNVVVRSPWL
SQGYLKDHKASERLWEGGYLHTGDVAVRDELGYLKITDRSKDVIKVAGEWVSSLELEDIVAHHPAVAEVAVIGKPDEKWG
ERPLALVVLKPTEGTKVTDKEIAHHVREYADKGVVSKQVVLVKVKLVPSIDKTSVGKINKVALREKYL

Sequences:

>Translated_548_residues
MSDLLIPRTASAYNYPLLIKNLLLYPVVDNPDQEIVYRDLYRGNYRQLRERVKRLANMLTGLGVKPGQTVAVMDWDSHRY
LELFFAVPMIGAVLHTINVRLSAEQILYTIDHAEDDVLLVNSEFLPIMEQIRGRIDNVRTYILISDDGMTECSTIPACGE
YEQLLAQASPEFEFPDLDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSLGLLATLGSSTSHACLHRDDVYMPITPMFHV
HAWGVPYIATMLGVKQVYPGRYLPETLLELKEKEGVSFSHCVPTILHMLLKHPLAEKIDLRGWKLIIGGAALSRNLCVES
LRLGIDVFTGYGMSETCPILTISKLTPEMLELSHAEQAEIRCKTGLALALVDLRVVDSNFNELPRDGVSAGNVVVRSPWL
SQGYLKDHKASERLWEGGYLHTGDVAVRDELGYLKITDRSKDVIKVAGEWVSSLELEDIVAHHPAVAEVAVIGKPDEKWG
ERPLALVVLKPTEGTKVTDKEIAHHVREYADKGVVSKQVVLVKVKLVPSIDKTSVGKINKVALREKYL
>Mature_547_residues
SDLLIPRTASAYNYPLLIKNLLLYPVVDNPDQEIVYRDLYRGNYRQLRERVKRLANMLTGLGVKPGQTVAVMDWDSHRYL
ELFFAVPMIGAVLHTINVRLSAEQILYTIDHAEDDVLLVNSEFLPIMEQIRGRIDNVRTYILISDDGMTECSTIPACGEY
EQLLAQASPEFEFPDLDENTRATTFYTTGTTGMPKGVYFSHRQLVLHSLGLLATLGSSTSHACLHRDDVYMPITPMFHVH
AWGVPYIATMLGVKQVYPGRYLPETLLELKEKEGVSFSHCVPTILHMLLKHPLAEKIDLRGWKLIIGGAALSRNLCVESL
RLGIDVFTGYGMSETCPILTISKLTPEMLELSHAEQAEIRCKTGLALALVDLRVVDSNFNELPRDGVSAGNVVVRSPWLS
QGYLKDHKASERLWEGGYLHTGDVAVRDELGYLKITDRSKDVIKVAGEWVSSLELEDIVAHHPAVAEVAVIGKPDEKWGE
RPLALVVLKPTEGTKVTDKEIAHHVREYADKGVVSKQVVLVKVKLVPSIDKTSVGKINKVALREKYL

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=519, Percent_Identity=25.0481695568401, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=23.3766233766234, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=23.3766233766234, Blast_Score=116, Evalue=7e-26,
Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=24.8638838475499, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI42544132, Length=520, Percent_Identity=22.8846153846154, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI58082049, Length=526, Percent_Identity=22.6235741444867, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28416953, Length=579, Percent_Identity=25.9067357512953, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI115511026, Length=524, Percent_Identity=25, Blast_Score=110, Evalue=3e-24,
Organism=Homo sapiens, GI187761345, Length=548, Percent_Identity=26.4598540145985, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI187761343, Length=548, Percent_Identity=26.4598540145985, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI13375727, Length=548, Percent_Identity=25.5474452554745, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI122937307, Length=527, Percent_Identity=23.719165085389, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI57165410, Length=332, Percent_Identity=27.4096385542169, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI57165412, Length=336, Percent_Identity=27.3809523809524, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI221142682, Length=478, Percent_Identity=25.3138075313808, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI145693145, Length=372, Percent_Identity=27.9569892473118, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788107, Length=529, Percent_Identity=25.3308128544423, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786810, Length=525, Percent_Identity=23.8095238095238, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=537, Percent_Identity=24.9534450651769, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1786529, Length=542, Percent_Identity=26.0147601476015, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1788595, Length=447, Percent_Identity=21.923937360179, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17559526, Length=539, Percent_Identity=25.7884972170686, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531443, Length=517, Percent_Identity=23.9845261121857, Blast_Score=130, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=518, Percent_Identity=23.5521235521236, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=556, Percent_Identity=25.7194244604317, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17557194, Length=517, Percent_Identity=22.8239845261122, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71994703, Length=570, Percent_Identity=23.6842105263158, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=589, Percent_Identity=23.2597623089983, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994690, Length=589, Percent_Identity=23.2597623089983, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71996755, Length=541, Percent_Identity=24.9537892791128, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI32563687, Length=517, Percent_Identity=25.531914893617, Blast_Score=109, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17560140, Length=509, Percent_Identity=22.5933202357564, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=372, Percent_Identity=25.5376344086022, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=377, Percent_Identity=26.790450928382, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17556552, Length=516, Percent_Identity=24.2248062015504, Blast_Score=86, Evalue=7e-17,
Organism=Saccharomyces cerevisiae, GI6319699, Length=379, Percent_Identity=26.3852242744063, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6319264, Length=557, Percent_Identity=25.3141831238779, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323182, Length=592, Percent_Identity=24.3243243243243, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI21355181, Length=508, Percent_Identity=26.5748031496063, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18859661, Length=374, Percent_Identity=29.9465240641711, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI19922652, Length=506, Percent_Identity=24.5059288537549, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24648257, Length=555, Percent_Identity=24.3243243243243, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI20130357, Length=373, Percent_Identity=25.7372654155496, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24581924, Length=549, Percent_Identity=24.7723132969035, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24648260, Length=555, Percent_Identity=22.3423423423423, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24583465, Length=478, Percent_Identity=22.1757322175732, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583463, Length=478, Percent_Identity=22.1757322175732, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364798, Length=475, Percent_Identity=22.1052631578947, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653035, Length=329, Percent_Identity=25.8358662613982, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI21356947, Length=355, Percent_Identity=26.1971830985915, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI21355225, Length=553, Percent_Identity=23.1464737793852, Blast_Score=79, Evalue=6e-15,
Organism=Drosophila melanogaster, GI161076582, Length=336, Percent_Identity=25, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648253, Length=519, Percent_Identity=21.3872832369942, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24648255, Length=519, Percent_Identity=21.3872832369942, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21356441, Length=367, Percent_Identity=23.9782016348774, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI62472339, Length=164, Percent_Identity=30.4878048780488, Blast_Score=67, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24667955, Length=164, Percent_Identity=30.4878048780488, Blast_Score=67, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24667959, Length=164, Percent_Identity=30.4878048780488, Blast_Score=66, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 61274; Mature: 61142

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLIPRTASAYNYPLLIKNLLLYPVVDNPDQEIVYRDLYRGNYRQLRERVKRLANMLT
CCCCCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GLGVKPGQTVAVMDWDSHRYLELFFAVPMIGAVLHTINVRLSAEQILYTIDHAEDDVLLV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHEEEEEECCCCCEEEE
NSEFLPIMEQIRGRIDNVRTYILISDDGMTECSTIPACGEYEQLLAQASPEFEFPDLDEN
CCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
TRATTFYTTGTTGMPKGVYFSHRQLVLHSLGLLATLGSSTSHACLHRDDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCEEE
HAWGVPYIATMLGVKQVYPGRYLPETLLELKEKEGVSFSHCVPTILHMLLKHPLAEKIDL
EECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCC
RGWKLIIGGAALSRNLCVESLRLGIDVFTGYGMSETCPILTISKLTPEMLELSHAEQAEI
CCEEEEEECHHHHHHHHHHHHHHCHHEEECCCCCCCCCEEEEHHCCHHHHHHCCCCHHHE
RCKTGLALALVDLRVVDSNFNELPRDGVSAGNVVVRSPWLSQGYLKDHKASERLWEGGYL
EECCCCEEEEEEEEEECCCHHHCCCCCCCCCCEEEECCCCCCCCHHHCHHHHHHHCCCCE
HTGDVAVRDELGYLKITDRSKDVIKVAGEWVSSLELEDIVAHHPAVAEVAVIGKPDEKWG
ECCCEEEECCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHC
ERPLALVVLKPTEGTKVTDKEIAHHVREYADKGVVSKQVVLVKVKLVPSIDKTSVGKINK
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHH
VALREKYL
HHHHHCCC
>Mature Secondary Structure 
SDLLIPRTASAYNYPLLIKNLLLYPVVDNPDQEIVYRDLYRGNYRQLRERVKRLANMLT
CCCCCCCCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
GLGVKPGQTVAVMDWDSHRYLELFFAVPMIGAVLHTINVRLSAEQILYTIDHAEDDVLLV
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEEEHHHEEEEEECCCCCEEEE
NSEFLPIMEQIRGRIDNVRTYILISDDGMTECSTIPACGEYEQLLAQASPEFEFPDLDEN
CCCCHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCC
TRATTFYTTGTTGMPKGVYFSHRQLVLHSLGLLATLGSSTSHACLHRDDVYMPITPMFHV
CCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCEEEECCCEEECCCCCEEE
HAWGVPYIATMLGVKQVYPGRYLPETLLELKEKEGVSFSHCVPTILHMLLKHPLAEKIDL
EECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHCC
RGWKLIIGGAALSRNLCVESLRLGIDVFTGYGMSETCPILTISKLTPEMLELSHAEQAEI
CCEEEEEECHHHHHHHHHHHHHHCHHEEECCCCCCCCCEEEEHHCCHHHHHHCCCCHHHE
RCKTGLALALVDLRVVDSNFNELPRDGVSAGNVVVRSPWLSQGYLKDHKASERLWEGGYL
EECCCCEEEEEEEEEECCCHHHCCCCCCCCCCEEEECCCCCCCCHHHCHHHHHHHCCCCE
HTGDVAVRDELGYLKITDRSKDVIKVAGEWVSSLELEDIVAHHPAVAEVAVIGKPDEKWG
ECCCEEEECCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEEEEECCCCHHHC
ERPLALVVLKPTEGTKVTDKEIAHHVREYADKGVVSKQVVLVKVKLVPSIDKTSVGKINK
CCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCCCCCHHH
VALREKYL
HHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]