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The map label for this gene is thrS

Identifier: 197118372

GI number: 197118372

Start: 2295267

End: 2297177

Strand: Direct

Name: thrS

Synonym: Gbem_1990

Alternate gene names: 197118372

Gene position: 2295267-2297177 (Clockwise)

Preceding gene: 197118371

Following gene: 308535278

Centisome position: 49.73

GC content: 59.08

Gene sequence:

>1911_bases
ATGAACGAAATAAACGTCACGCTACCGGATGGTTCCCAAAGACCCTTGCCTGCAGGTGCCTCCATTTTCGATCTTGCCGC
ATCCATCGGAGCGGGGCTTGCCAAGGCGGCCATCGCCGGAAAGATCGACGGCAACCTGGTCGACCTCAATACGCCTCTTG
CCGACGGCGCGCGCGTGGAGATCATCACCGAGAAAAGCCCCGAGGCGCTGGAGATCATCAGGCACTCCACCTCGCACCTT
ATGGCGCAGGCCGTAAAGGCGCTCTTTCCGCAGGCGAAGGTGACCATCGGTCCCGCCATCGAGACCGGTTTTTACTACGA
CTTCGACGTCGACCACCCCTTCACCCCGGAAGACCTCGAGAAGATAGAAGAAAAGATGCGCGAGCTCGCCAAGGCCGATC
TCAAGATCGAGCGCAAGGAGCTCACCAGCGCCGACGCCATCGCCCTCTTCAAAGGGATGGGTGAGAACTACAAGGTCGAA
CTGATCGAGGACTTGGGGGCCGACAAGGTTTCGCTTTACAGCCAGGGCGATTTCGTCGACCTCTGCCGCGGGCCGCATCT
TCCCAAGACCTCTTTCATCAAGGCCTTCAAGCTCACCTCCATAGCAGGCGCCTACTGGCGCGGCGACGAGAAGCGCCCCA
TGCTGCAGCGCGTCTACGGCACCGCCTTTGGCGATAAGAAGGAACTGGAAGCCTACCTCGCCAGGATCGAAGAGGCGAAA
AAGCGCGACCACCGCAAGCTCGGCCGCGAACTGGATCTCTTCTCCTTCAACGACGAGGTCGGCGCAGGACTCGTGATCTG
GCACCCCAAAGGGGCGATGCTGCGCACTATCCTCGAGGACTTCGAGAGGAAGGAGCACTTAAAGCGCGGCTACGACATCG
TCCTGGGTCCGCAGATCCTCAAGACCGAACTCTGGCAGCGCTCCGGGCACTACGAGAACTACCGCGAGAACATGTACTTC
ACCACGGTGGACGAGCAGAGCTACGGCGTGAAGCCGATGAACTGCCTGGCCCACATGATGATCTACCGCTCGCAGCTTCG
CTCTTACCGCGACCTGCCGCTGCGCTACTTCGAGCTCGGCACGGTGCACCGCCATGAGCGCGCCGGCGTTCTGCACGGCC
TTCTGCGCGTGCGCGGCTTCACCCAGGACGACGCCCATATCCTCTGTACCCCGGAGCAGCTCGATGCCGAGATCAAAGGG
GTCATCCAGTTCGTCACCGAGGTGATGGGTATCTTCGGCTTCGAGTTCGAGATGGAGCTTTCCACCCGTCCCGAAAAGTC
GATCGGTTCCGACGACGCCTGGGAGCTCGCCACCAACGCTTTGTTGAACGCGCTCAAGGATTCCGGCCGCCCTTACGAAA
TCAACGAGGGGGACGGCGCATTCTACGGTCCCAAGATCGACATCAAGCTGCGTGACGCGCTTGACAGAAGATGGCAATGT
GCTACAATCCAGTGCGATTTTACCCTCCCTGAGCGTTTCGATCTCACCTATGTCGACGCAGACGGTGAAAAAAAGCGCCC
CGTCATGGTGCACAGGGTTATTCTGGGTGCCATCGAACGCTTCATCGGTGTCCTCATCGAACATTTCGCTGGAAACTTCC
CGACTTGGTTGGCACCGGTTCAGGCGACCATCGTTACGGTCACCGACAACCAGATTCCGTACGCGCAGGCAGCGTTCGAC
AAGCTGCGCGCGGCCGGGATAAGGGTGCAGAAGGATTTCAGAAACGAGAAGCTCGGCTTCAAGATCCGCGAAGCCCAGCT
CCAGAAGATACCGTACATGCTGGTTGTGGGGGACAAGGAAGTCGAGAGCGGTATGCTGGCGCCGCGTTTCCGCGACGGCA
AGAACCTCGAGTCCATGACCCCGGAGCAGTTTGTTTCCTTTATCGAAAGCGAAGTCAAAAGTTATAAATAA

Upstream 100 bases:

>100_bases
CTACCAATCAAAAGAGACCTTCAGTACGAGGTTCGACGGAGGCATCGAGTTTCGATGCCTCTTTCTGTTTGTCTTAATGA
TTTTTCAAAAGGGGCTTTGC

Downstream 100 bases:

>100_bases
CAATAGGAGGTGGCGTCATAGCTAAACCTACAGTCAACATCAATCAGACCATCAGGGCCAAAGAGGTAAGGGTAGTAGGT
GCCGAAAGTGAGCAGCTTGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MNEINVTLPDGSQRPLPAGASIFDLAASIGAGLAKAAIAGKIDGNLVDLNTPLADGARVEIITEKSPEALEIIRHSTSHL
MAQAVKALFPQAKVTIGPAIETGFYYDFDVDHPFTPEDLEKIEEKMRELAKADLKIERKELTSADAIALFKGMGENYKVE
LIEDLGADKVSLYSQGDFVDLCRGPHLPKTSFIKAFKLTSIAGAYWRGDEKRPMLQRVYGTAFGDKKELEAYLARIEEAK
KRDHRKLGRELDLFSFNDEVGAGLVIWHPKGAMLRTILEDFERKEHLKRGYDIVLGPQILKTELWQRSGHYENYRENMYF
TTVDEQSYGVKPMNCLAHMMIYRSQLRSYRDLPLRYFELGTVHRHERAGVLHGLLRVRGFTQDDAHILCTPEQLDAEIKG
VIQFVTEVMGIFGFEFEMELSTRPEKSIGSDDAWELATNALLNALKDSGRPYEINEGDGAFYGPKIDIKLRDALDRRWQC
ATIQCDFTLPERFDLTYVDADGEKKRPVMVHRVILGAIERFIGVLIEHFAGNFPTWLAPVQATIVTVTDNQIPYAQAAFD
KLRAAGIRVQKDFRNEKLGFKIREAQLQKIPYMLVVGDKEVESGMLAPRFRDGKNLESMTPEQFVSFIESEVKSYK

Sequences:

>Translated_636_residues
MNEINVTLPDGSQRPLPAGASIFDLAASIGAGLAKAAIAGKIDGNLVDLNTPLADGARVEIITEKSPEALEIIRHSTSHL
MAQAVKALFPQAKVTIGPAIETGFYYDFDVDHPFTPEDLEKIEEKMRELAKADLKIERKELTSADAIALFKGMGENYKVE
LIEDLGADKVSLYSQGDFVDLCRGPHLPKTSFIKAFKLTSIAGAYWRGDEKRPMLQRVYGTAFGDKKELEAYLARIEEAK
KRDHRKLGRELDLFSFNDEVGAGLVIWHPKGAMLRTILEDFERKEHLKRGYDIVLGPQILKTELWQRSGHYENYRENMYF
TTVDEQSYGVKPMNCLAHMMIYRSQLRSYRDLPLRYFELGTVHRHERAGVLHGLLRVRGFTQDDAHILCTPEQLDAEIKG
VIQFVTEVMGIFGFEFEMELSTRPEKSIGSDDAWELATNALLNALKDSGRPYEINEGDGAFYGPKIDIKLRDALDRRWQC
ATIQCDFTLPERFDLTYVDADGEKKRPVMVHRVILGAIERFIGVLIEHFAGNFPTWLAPVQATIVTVTDNQIPYAQAAFD
KLRAAGIRVQKDFRNEKLGFKIREAQLQKIPYMLVVGDKEVESGMLAPRFRDGKNLESMTPEQFVSFIESEVKSYK
>Mature_636_residues
MNEINVTLPDGSQRPLPAGASIFDLAASIGAGLAKAAIAGKIDGNLVDLNTPLADGARVEIITEKSPEALEIIRHSTSHL
MAQAVKALFPQAKVTIGPAIETGFYYDFDVDHPFTPEDLEKIEEKMRELAKADLKIERKELTSADAIALFKGMGENYKVE
LIEDLGADKVSLYSQGDFVDLCRGPHLPKTSFIKAFKLTSIAGAYWRGDEKRPMLQRVYGTAFGDKKELEAYLARIEEAK
KRDHRKLGRELDLFSFNDEVGAGLVIWHPKGAMLRTILEDFERKEHLKRGYDIVLGPQILKTELWQRSGHYENYRENMYF
TTVDEQSYGVKPMNCLAHMMIYRSQLRSYRDLPLRYFELGTVHRHERAGVLHGLLRVRGFTQDDAHILCTPEQLDAEIKG
VIQFVTEVMGIFGFEFEMELSTRPEKSIGSDDAWELATNALLNALKDSGRPYEINEGDGAFYGPKIDIKLRDALDRRWQC
ATIQCDFTLPERFDLTYVDADGEKKRPVMVHRVILGAIERFIGVLIEHFAGNFPTWLAPVQATIVTVTDNQIPYAQAAFD
KLRAAGIRVQKDFRNEKLGFKIREAQLQKIPYMLVVGDKEVESGMLAPRFRDGKNLESMTPEQFVSFIESEVKSYK

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=615, Percent_Identity=40.9756097560976, Blast_Score=480, Evalue=1e-135,
Organism=Homo sapiens, GI38570111, Length=595, Percent_Identity=40.3361344537815, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI20070344, Length=649, Percent_Identity=37.904468412943, Blast_Score=412, Evalue=1e-115,
Organism=Escherichia coli, GI1788013, Length=632, Percent_Identity=50.7911392405063, Blast_Score=666, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=620, Percent_Identity=40.1612903225806, Blast_Score=494, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71984192, Length=620, Percent_Identity=40.1612903225806, Blast_Score=494, Evalue=1e-140,
Organism=Saccharomyces cerevisiae, GI6322112, Length=640, Percent_Identity=40.46875, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322654, Length=418, Percent_Identity=40.4306220095694, Blast_Score=293, Evalue=5e-80,
Organism=Drosophila melanogaster, GI24583842, Length=615, Percent_Identity=41.7886178861789, Blast_Score=507, Evalue=1e-143,
Organism=Drosophila melanogaster, GI28574141, Length=615, Percent_Identity=41.7886178861789, Blast_Score=507, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24583839, Length=615, Percent_Identity=41.7886178861789, Blast_Score=506, Evalue=1e-143,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_GEOBB (B5EBX9)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138799.1
- ProteinModelPortal:   B5EBX9
- SMR:   B5EBX9
- GeneID:   6781982
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1990
- HOGENOM:   HBG352811
- OMA:   FHPKGMV
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 71954; Mature: 71954

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEINVTLPDGSQRPLPAGASIFDLAASIGAGLAKAAIAGKIDGNLVDLNTPLADGARVE
CCCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCEEE
IITEKSPEALEIIRHSTSHLMAQAVKALFPQAKVTIGPAIETGFYYDFDVDHPFTPEDLE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCEEEEECCCCCCCHHHHH
KIEEKMRELAKADLKIERKELTSADAIALFKGMGENYKVELIEDLGADKVSLYSQGDFVD
HHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHCCCCCCEEEEHHHCCCCEEEEECCCCCHH
LCRGPHLPKTSFIKAFKLTSIAGAYWRGDEKRPMLQRVYGTAFGDKKELEAYLARIEEAK
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRDHRKLGRELDLFSFNDEVGAGLVIWHPKGAMLRTILEDFERKEHLKRGYDIVLGPQIL
HHHHHHHCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHH
KTELWQRSGHYENYRENMYFTTVDEQSYGVKPMNCLAHMMIYRSQLRSYRDLPLRYFELG
HHHHHHHCCCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECC
TVHRHERAGVLHGLLRVRGFTQDDAHILCTPEQLDAEIKGVIQFVTEVMGIFGFEFEMEL
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
STRPEKSIGSDDAWELATNALLNALKDSGRPYEINEGDGAFYGPKIDIKLRDALDRRWQC
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEEEEEEHHHHCCCEEE
ATIQCDFTLPERFDLTYVDADGEKKRPVMVHRVILGAIERFIGVLIEHFAGNFPTWLAPV
EEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QATIVTVTDNQIPYAQAAFDKLRAAGIRVQKDFRNEKLGFKIREAQLQKIPYMLVVGDKE
EEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHCCCCCCCEEHHHHHHHCCEEEEECCCH
VESGMLAPRFRDGKNLESMTPEQFVSFIESEVKSYK
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNEINVTLPDGSQRPLPAGASIFDLAASIGAGLAKAAIAGKIDGNLVDLNTPLADGARVE
CCCEEEECCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCCEEECCCCCCCCCEEE
IITEKSPEALEIIRHSTSHLMAQAVKALFPQAKVTIGPAIETGFYYDFDVDHPFTPEDLE
EEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCEEECCEEEEECCCCCCCHHHHH
KIEEKMRELAKADLKIERKELTSADAIALFKGMGENYKVELIEDLGADKVSLYSQGDFVD
HHHHHHHHHHHHCCEEEHHHHCCHHHHHHHHCCCCCCEEEEHHHCCCCEEEEECCCCCHH
LCRGPHLPKTSFIKAFKLTSIAGAYWRGDEKRPMLQRVYGTAFGDKKELEAYLARIEEAK
HHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KRDHRKLGRELDLFSFNDEVGAGLVIWHPKGAMLRTILEDFERKEHLKRGYDIVLGPQIL
HHHHHHHCCCCCEEECCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHH
KTELWQRSGHYENYRENMYFTTVDEQSYGVKPMNCLAHMMIYRSQLRSYRDLPLRYFELG
HHHHHHHCCCCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEECC
TVHRHERAGVLHGLLRVRGFTQDDAHILCTPEQLDAEIKGVIQFVTEVMGIFGFEFEMEL
CCHHHHHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
STRPEKSIGSDDAWELATNALLNALKDSGRPYEINEGDGAFYGPKIDIKLRDALDRRWQC
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCEECCEEEEEEHHHHCCCEEE
ATIQCDFTLPERFDLTYVDADGEKKRPVMVHRVILGAIERFIGVLIEHFAGNFPTWLAPV
EEEEEECCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
QATIVTVTDNQIPYAQAAFDKLRAAGIRVQKDFRNEKLGFKIREAQLQKIPYMLVVGDKE
EEEEEEEECCCCCHHHHHHHHHHHHCCEEEHHHCCCCCCCEEHHHHHHHCCEEEEECCCH
VESGMLAPRFRDGKNLESMTPEQFVSFIESEVKSYK
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA