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The map label for this gene is aroF [H]

Identifier: 197118345

GI number: 197118345

Start: 2266317

End: 2267390

Strand: Reverse

Name: aroF [H]

Synonym: Gbem_1962

Alternate gene names: 197118345

Gene position: 2267390-2266317 (Counterclockwise)

Preceding gene: 197118346

Following gene: 308535276

Centisome position: 49.13

GC content: 61.27

Gene sequence:

>1074_bases
ATGATCAAGACCAACAATCTCAAGGTAAAAAGCATCACTCCGATCATTGCACCTACCGATCTCAGACAAGTCTTCCCCAT
TTCCGAGAAATCCGGGACCTGCGTGAGCAACAGCCGCGCGGCCATCACCAGGATCTTGAAGGGAGAGGACAAGCGGCTCA
TGGTGGTCGTCGGCCCCTGCTCCATTCACGACCCCAAGGGAGCCCTGGAGTACGCCGAGAAGCTCGCGGCGCTCGCCAAG
GAGGTCTCCGAGGAAATGCTGCTGATCATGCGCGTGTACTTCGAGAAGCCGCGCACCACCATCGGGTGGAAGGGGCTCAT
CAACGACCCGGACATGAACGGCACCCACCAGATCTCCAAGGGGCTTGGCATCGCCCGCAGCCTTCTCTGCAAGATCACCG
AGATGGGGCTGCCGGTCGCGACCGAGATGCTCGACCCGATCACCCCCGAGTACCTGGCCGATCTCCTCTCCTGGGGCGCC
ATCGGGGCCCGCACCACCGAATCACAGACCCACCGCGAGATGGCAAGCGGCCTCTCCTTCGCGATAGGGTTTAAAAACGG
CACCGACGGGAACCTCCAGATCGCCATCGACGCCATGAAGGCAGCGCTTCATTCACACAGTTTCCTCGGCATCAACCGCG
ACGGCCTGACCTCCATCATCCAGACCACCGGCAACCCCGACGTGCACATGGTCCTGCGCGGCGGGAGCAAGAAGCCGAAC
TACTCCCCGGAGGACATCGCCAAATCCGAGGAGATGATCGCCAAGGCGGGCCTGACCCCGACCATGATGGTCGATTGCAG
CCACGGCAACTCCGAGAAGAAGTACGAGCGGCAGCCCGAGGTGATGAAGAGCGTGATCGAACAGATCGCCGCCGGCAACC
GCAGCATCTCCGGCGTGATGATCGAGAGCTATCTGAAGGAAGGGAACCAGCCGATGCCGAAAGACGGCGATCCCTCCTCC
TTGACCTACGGCGTATCGATCACCGACAGCTGCATCAGCTGGGAGACCACCGAGGCCACCCTGCGCGAAGCCCACCGCAG
ATTGAAAGCATGCGGCGGCAGGAAGATTTCTTAG

Upstream 100 bases:

>100_bases
CTCGGCAAAATTCACCACGCCGAGGGTATTTTTTTTTGACACTACATGAGAAACGCGCTAGATTACAGCACATTAAAAAC
TGCTAGCGCGGGAGTCTCAG

Downstream 100 bases:

>100_bases
TTAGTTAAAGGCTACAGGCGGAACACAGAGTTCACGGAGGTTCGCAGAGGACGCTGAGGAAGAACAAGGCATTGAACAGG
GATGAAGGGGATACAGGGCC

Product: phospho-2-dehydro-3-deoxyheptonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase; DAHP synthase; Phospho-2-keto-3-deoxyheptonate aldolase [H]

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MIKTNNLKVKSITPIIAPTDLRQVFPISEKSGTCVSNSRAAITRILKGEDKRLMVVVGPCSIHDPKGALEYAEKLAALAK
EVSEEMLLIMRVYFEKPRTTIGWKGLINDPDMNGTHQISKGLGIARSLLCKITEMGLPVATEMLDPITPEYLADLLSWGA
IGARTTESQTHREMASGLSFAIGFKNGTDGNLQIAIDAMKAALHSHSFLGINRDGLTSIIQTTGNPDVHMVLRGGSKKPN
YSPEDIAKSEEMIAKAGLTPTMMVDCSHGNSEKKYERQPEVMKSVIEQIAAGNRSISGVMIESYLKEGNQPMPKDGDPSS
LTYGVSITDSCISWETTEATLREAHRRLKACGGRKIS

Sequences:

>Translated_357_residues
MIKTNNLKVKSITPIIAPTDLRQVFPISEKSGTCVSNSRAAITRILKGEDKRLMVVVGPCSIHDPKGALEYAEKLAALAK
EVSEEMLLIMRVYFEKPRTTIGWKGLINDPDMNGTHQISKGLGIARSLLCKITEMGLPVATEMLDPITPEYLADLLSWGA
IGARTTESQTHREMASGLSFAIGFKNGTDGNLQIAIDAMKAALHSHSFLGINRDGLTSIIQTTGNPDVHMVLRGGSKKPN
YSPEDIAKSEEMIAKAGLTPTMMVDCSHGNSEKKYERQPEVMKSVIEQIAAGNRSISGVMIESYLKEGNQPMPKDGDPSS
LTYGVSITDSCISWETTEATLREAHRRLKACGGRKIS
>Mature_357_residues
MIKTNNLKVKSITPIIAPTDLRQVFPISEKSGTCVSNSRAAITRILKGEDKRLMVVVGPCSIHDPKGALEYAEKLAALAK
EVSEEMLLIMRVYFEKPRTTIGWKGLINDPDMNGTHQISKGLGIARSLLCKITEMGLPVATEMLDPITPEYLADLLSWGA
IGARTTESQTHREMASGLSFAIGFKNGTDGNLQIAIDAMKAALHSHSFLGINRDGLTSIIQTTGNPDVHMVLRGGSKKPN
YSPEDIAKSEEMIAKAGLTPTMMVDCSHGNSEKKYERQPEVMKSVIEQIAAGNRSISGVMIESYLKEGNQPMPKDGDPSS
LTYGVSITDSCISWETTEATLREAHRRLKACGGRKIS

Specific function: Stereospecific condensation of phosphoenolpyruvate (PEP) and D-erythrose-4-phosphate (E4P) giving rise to 3-deoxy-D- arabino-heptulosonate-7-phosphate (DAHP) [H]

COG id: COG0722

COG function: function code E; 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I DAHP synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788953, Length=347, Percent_Identity=57.9250720461095, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1786969, Length=348, Percent_Identity=54.0229885057471, Blast_Score=392, Evalue=1e-110,
Organism=Escherichia coli, GI1787996, Length=338, Percent_Identity=49.7041420118343, Blast_Score=362, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6320240, Length=343, Percent_Identity=51.3119533527697, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6319726, Length=338, Percent_Identity=50.2958579881657, Blast_Score=357, Evalue=2e-99,

Paralogues:

None

Copy number: 2,200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006219 [H]

Pfam domain/function: PF00793 DAHP_synth_1 [H]

EC number: =2.5.1.54 [H]

Molecular weight: Translated: 38817; Mature: 38817

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKTNNLKVKSITPIIAPTDLRQVFPISEKSGTCVSNSRAAITRILKGEDKRLMVVVGPC
CCCCCCEEEEEECCEECCCCHHHHCCCCCCCCCEECCCHHHHHHHHCCCCCEEEEEECCC
SIHDPKGALEYAEKLAALAKEVSEEMLLIMRVYFEKPRTTIGWKGLINDPDMNGTHQISK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
GLGIARSLLCKITEMGLPVATEMLDPITPEYLADLLSWGAIGARTTESQTHREMASGLSF
HHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
AIGFKNGTDGNLQIAIDAMKAALHSHSFLGINRDGLTSIIQTTGNPDVHMVLRGGSKKPN
EEECCCCCCCCEEEEHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCC
YSPEDIAKSEEMIAKAGLTPTMMVDCSHGNSEKKYERQPEVMKSVIEQIAAGNRSISGVM
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
IESYLKEGNQPMPKDGDPSSLTYGVSITDSCISWETTEATLREAHRRLKACGGRKIS
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIKTNNLKVKSITPIIAPTDLRQVFPISEKSGTCVSNSRAAITRILKGEDKRLMVVVGPC
CCCCCCEEEEEECCEECCCCHHHHCCCCCCCCCEECCCHHHHHHHHCCCCCEEEEEECCC
SIHDPKGALEYAEKLAALAKEVSEEMLLIMRVYFEKPRTTIGWKGLINDPDMNGTHQISK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHH
GLGIARSLLCKITEMGLPVATEMLDPITPEYLADLLSWGAIGARTTESQTHREMASGLSF
HHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEE
AIGFKNGTDGNLQIAIDAMKAALHSHSFLGINRDGLTSIIQTTGNPDVHMVLRGGSKKPN
EEECCCCCCCCEEEEHHHHHHHHHCCCEEECCHHHHHHHHHCCCCCCEEEEEECCCCCCC
YSPEDIAKSEEMIAKAGLTPTMMVDCSHGNSEKKYERQPEVMKSVIEQIAAGNRSISGVM
CCHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCCHHHHHHHHHHHHCCCCCCHHHH
IESYLKEGNQPMPKDGDPSSLTYGVSITDSCISWETTEATLREAHRRLKACGGRKIS
HHHHHHCCCCCCCCCCCCCCEEEEEEECCCHHCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6146618; 6396419; 9205837; 9278503; 1977738; 6104668; 2857723; 9056491 [H]