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The map label for this gene is pncA [H]

Identifier: 197118346

GI number: 197118346

Start: 2267507

End: 2268139

Strand: Reverse

Name: pncA [H]

Synonym: Gbem_1963

Alternate gene names: 197118346

Gene position: 2268139-2267507 (Counterclockwise)

Preceding gene: 197118347

Following gene: 197118345

Centisome position: 49.15

GC content: 65.72

Gene sequence:

>633_bases
ATGCAACGAAGAGGAGCACTTCTTGTTGTCGACGCGCAGGTGGATTTCTGTCCGGGCGGGGCGCTCCCCGTCCCCCGGGG
CGACCAGGTGATCCACGCACTGAACCGGTACCTGGATCTGTTCAAGCGCAGGAGCGCGCCCATCTTCGCCTCGCGCGACT
GGCATCCGAAGCAGAGCAAGCACTTCAAGGAAAACGGCGGAGAATGGCCCGCCCACTGCGTCCAGGGAACCCTCGGCGCC
GAATTCCACCCGGCACTCGTACTTCCAAGCGACACCATCGTCATCTCCAAGGGGATGGCCTCCTGGGACGACGGATACTC
GGCCATGCAGGGGGTAACCGAGAACGGCACCCCCTTCACCATGCTGCTGCGGCACATGGAGCTGGACCGGCTCTACGTCG
GGGGGCTCGCCACCGATTTCTGCGTGAGACAGACGGTGCTCGAAGCCTTGAAAGCGGGATTCGACGTCACCCTCCTGACC
GATGCCGTAGGGGGGGTCGACCTCAGGCCGGGGGATTCTGAGCGGGCCGTGGCAGAGATGATCCACTCCGGCGCCGACGT
CACCACCCTCGCCTCCATCGGCACCGCGGTGGCGTGGGATGGGGCGCCGCAGCGGCAGCAGGGAAGCGGATAG

Upstream 100 bases:

>100_bases
GAGCCAGGGGCTGAACACCCTGCGGCTCAAACTCATGTCCCGGATTCAGAACCGGTATGCGGCGGGTGAAGCAGACGGGG
ATACGGCATAGGAGGCGATA

Downstream 100 bases:

>100_bases
CAAACAAAAATATACCCTCGGCAAAATTCACCACGCCGAGGGTATTTTTTTTTGACACTACATGAGAAACGCGCTAGATT
ACAGCACATTAAAAACTGCT

Product: nicotinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MQRRGALLVVDAQVDFCPGGALPVPRGDQVIHALNRYLDLFKRRSAPIFASRDWHPKQSKHFKENGGEWPAHCVQGTLGA
EFHPALVLPSDTIVISKGMASWDDGYSAMQGVTENGTPFTMLLRHMELDRLYVGGLATDFCVRQTVLEALKAGFDVTLLT
DAVGGVDLRPGDSERAVAEMIHSGADVTTLASIGTAVAWDGAPQRQQGSG

Sequences:

>Translated_210_residues
MQRRGALLVVDAQVDFCPGGALPVPRGDQVIHALNRYLDLFKRRSAPIFASRDWHPKQSKHFKENGGEWPAHCVQGTLGA
EFHPALVLPSDTIVISKGMASWDDGYSAMQGVTENGTPFTMLLRHMELDRLYVGGLATDFCVRQTVLEALKAGFDVTLLT
DAVGGVDLRPGDSERAVAEMIHSGADVTTLASIGTAVAWDGAPQRQQGSG
>Mature_210_residues
MQRRGALLVVDAQVDFCPGGALPVPRGDQVIHALNRYLDLFKRRSAPIFASRDWHPKQSKHFKENGGEWPAHCVQGTLGA
EFHPALVLPSDTIVISKGMASWDDGYSAMQGVTENGTPFTMLLRHMELDRLYVGGLATDFCVRQTVLEALKAGFDVTLLT
DAVGGVDLRPGDSERAVAEMIHSGADVTTLASIGTAVAWDGAPQRQQGSG

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=205, Percent_Identity=41.4634146341463, Blast_Score=144, Evalue=6e-36,
Organism=Saccharomyces cerevisiae, GI6321401, Length=204, Percent_Identity=25.4901960784314, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 22626; Mature: 22626

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRRGALLVVDAQVDFCPGGALPVPRGDQVIHALNRYLDLFKRRSAPIFASRDWHPKQSK
CCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
HFKENGGEWPAHCVQGTLGAEFHPALVLPSDTIVISKGMASWDDGYSAMQGVTENGTPFT
HHHHCCCCCCHHHHHCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHH
MLLRHMELDRLYVGGLATDFCVRQTVLEALKAGFDVTLLTDAVGGVDLRPGDSERAVAEM
HHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
IHSGADVTTLASIGTAVAWDGAPQRQQGSG
HHCCCCHHHHHHHCCCEEECCCCCCCCCCC
>Mature Secondary Structure
MQRRGALLVVDAQVDFCPGGALPVPRGDQVIHALNRYLDLFKRRSAPIFASRDWHPKQSK
CCCCCCEEEEECCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHH
HFKENGGEWPAHCVQGTLGAEFHPALVLPSDTIVISKGMASWDDGYSAMQGVTENGTPFT
HHHHCCCCCCHHHHHCCCCCCCCCEEECCCCEEEEECCCCCCCCCHHHHHCCCCCCCHHH
MLLRHMELDRLYVGGLATDFCVRQTVLEALKAGFDVTLLTDAVGGVDLRPGDSERAVAEM
HHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCHHHHHHHH
IHSGADVTTLASIGTAVAWDGAPQRQQGSG
HHCCCCHHHHHHHCCCEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]