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The map label for this gene is czcB [H]

Identifier: 197118323

GI number: 197118323

Start: 2241568

End: 2242854

Strand: Direct

Name: czcB [H]

Synonym: Gbem_1940

Alternate gene names: 197118323

Gene position: 2241568-2242854 (Clockwise)

Preceding gene: 197118322

Following gene: 197118324

Centisome position: 48.57

GC content: 65.03

Gene sequence:

>1287_bases
TTGAGCAAGAAAGCAAAGATCGTTGGATTAATTTTGGCCCTCGCCCTGACGGGAGCCTTTACCTATCGTTTCGTCCAGAT
GGGCGCCGCCGGTTCGGAACCAGGCGAGCATGCCGGCGAGGCCGGGCATACCGAAGCAAAGCATGAGGAAGAGGGGCACG
CCAAGGAGGAAAAGGGGCACGAGGAAGAGCATGCCGGAGTAGCGGGGCATGACGAACACGGAGCTGCCGGCAGCGTGACC
ATGAAACCCGAGATCCAGAAGCAAAACGGCGTGGTGCTGGCGGCCGCGAAGCCGCTGCGGCTGGCGGGAGTGATACGGGC
GACCGGCAAGGTCGAGGTGAACGCGGACCGGATCGCTCACGTGGCGCCCCGCATCCCGGGGAAGGTGGTTTCGGTGCGGG
CTTCCCTCGGCGACAGCGTGTCGGCGGGGCAGTCCCTGGCGACCCTCGACAGCGTCGAGGTCGGGGAAGCGATCGGCCGC
TACCGCCAGGCGAAGACCAGGCTCGCCCTGGCGCAGGGAAACATGGACCGGGTCAAGGCGCTTGTGGATAGGAAGATCGC
GGCGAGAAAGGACATACTCCAGGCCGAGACCGACTATAAGACGGCGCAGACCGAGCTCGCCACCGACGAGGAGCGCCTCT
CCCTGTACGGGGTATCCGTCGCAGAGGTAAGGGGAGGCGCCCGGAAGCCGCTATTGCCGGTCCGCTCCCCAATCGGCGGG
ATCGTCACCGAGAAACACGCCATCGTCGGCGAGCTTGCCGACCCATCCAAGAGCCTCTACACGGTCGCGGACCTCTCCTC
GGTCTGGGTGCTGGTGGATATAAACGAAAAGGACCTGGCGAAGGTCCACAAAGGGCAGGGGGCTGTGGTCACCGTCGGGG
CCTTCCCCGACCTGAAGCTCAAGGGGCGCATCACCTACATCGCCGACCTCGTGGACGAGTCGACCCGCACGGTGAAGGCG
CGGGTAGAAGTGACGAACCCCGGCCGGAAGCTGAAACCCGAGATGTTCGCCACGGCGGAACTTGCCCTGGCGGCAGACGC
CCCCCCGGTACTCGCGGTCCCGGAAGACGCTGTCCAGGATCTGGACGGCAAGAAGGTGGTCTTCGTCGCCGAAGAGGGGA
ACCGGTTCGAGGCGCGCCTGGTGCAGTTGGGAAGGAGCGCCGCGGGGCTCGTGGAGATCGCCTCCGGCCTGAAAGAGGGG
GAGAACTACGCCGCCAAGGGGGCCTTCATCCTCAAGTCGGAAGTGAAAAAAGGGGAAGTGGTCGACGAACACGGCCACGG
GAAATGA

Upstream 100 bases:

>100_bases
TCGAGGTGAACCAGGGGTACCTGGAAGCCCTGAACCAGCGGCAGCAGGCGCTTTTCAAACTGGAAGCGGCGGCGGCAACC
GAACTTACCGGAGGTGTGCA

Downstream 100 bases:

>100_bases
CCTGCAGCGCTTACAGTGATTTATAGCGAGGGACCATGCTAGAGAAAATAGTTTCTTATACACTGCGGCACAGGGCGATG
ATCCTCTTCCTGTCGCTCTT

Product: RND family efflux pump membrane fusion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSKKAKIVGLILALALTGAFTYRFVQMGAAGSEPGEHAGEAGHTEAKHEEEGHAKEEKGHEEEHAGVAGHDEHGAAGSVT
MKPEIQKQNGVVLAAAKPLRLAGVIRATGKVEVNADRIAHVAPRIPGKVVSVRASLGDSVSAGQSLATLDSVEVGEAIGR
YRQAKTRLALAQGNMDRVKALVDRKIAARKDILQAETDYKTAQTELATDEERLSLYGVSVAEVRGGARKPLLPVRSPIGG
IVTEKHAIVGELADPSKSLYTVADLSSVWVLVDINEKDLAKVHKGQGAVVTVGAFPDLKLKGRITYIADLVDESTRTVKA
RVEVTNPGRKLKPEMFATAELALAADAPPVLAVPEDAVQDLDGKKVVFVAEEGNRFEARLVQLGRSAAGLVEIASGLKEG
ENYAAKGAFILKSEVKKGEVVDEHGHGK

Sequences:

>Translated_428_residues
MSKKAKIVGLILALALTGAFTYRFVQMGAAGSEPGEHAGEAGHTEAKHEEEGHAKEEKGHEEEHAGVAGHDEHGAAGSVT
MKPEIQKQNGVVLAAAKPLRLAGVIRATGKVEVNADRIAHVAPRIPGKVVSVRASLGDSVSAGQSLATLDSVEVGEAIGR
YRQAKTRLALAQGNMDRVKALVDRKIAARKDILQAETDYKTAQTELATDEERLSLYGVSVAEVRGGARKPLLPVRSPIGG
IVTEKHAIVGELADPSKSLYTVADLSSVWVLVDINEKDLAKVHKGQGAVVTVGAFPDLKLKGRITYIADLVDESTRTVKA
RVEVTNPGRKLKPEMFATAELALAADAPPVLAVPEDAVQDLDGKKVVFVAEEGNRFEARLVQLGRSAAGLVEIASGLKEG
ENYAAKGAFILKSEVKKGEVVDEHGHGK
>Mature_427_residues
SKKAKIVGLILALALTGAFTYRFVQMGAAGSEPGEHAGEAGHTEAKHEEEGHAKEEKGHEEEHAGVAGHDEHGAAGSVTM
KPEIQKQNGVVLAAAKPLRLAGVIRATGKVEVNADRIAHVAPRIPGKVVSVRASLGDSVSAGQSLATLDSVEVGEAIGRY
RQAKTRLALAQGNMDRVKALVDRKIAARKDILQAETDYKTAQTELATDEERLSLYGVSVAEVRGGARKPLLPVRSPIGGI
VTEKHAIVGELADPSKSLYTVADLSSVWVLVDINEKDLAKVHKGQGAVVTVGAFPDLKLKGRITYIADLVDESTRTVKAR
VEVTNPGRKLKPEMFATAELALAADAPPVLAVPEDAVQDLDGKKVVFVAEEGNRFEARLVQLGRSAAGLVEIASGLKEGE
NYAAKGAFILKSEVKKGEVVDEHGHGK

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1786787, Length=351, Percent_Identity=27.0655270655271, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1789665, Length=309, Percent_Identity=25.8899676375405, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45233; Mature: 45102

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKAKIVGLILALALTGAFTYRFVQMGAAGSEPGEHAGEAGHTEAKHEEEGHAKEEKGH
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHCCCHHHCCC
EEEHAGVAGHDEHGAAGSVTMKPEIQKQNGVVLAAAKPLRLAGVIRATGKVEVNADRIAH
CHHHCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEEECCEEEECHHHHHH
VAPRIPGKVVSVRASLGDSVSAGQSLATLDSVEVGEAIGRYRQAKTRLALAQGNMDRVKA
HCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVDRKIAARKDILQAETDYKTAQTELATDEERLSLYGVSVAEVRGGARKPLLPVRSPIGG
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHEEECCCHHHHCCCCCCCCCCCCCCCCC
IVTEKHAIVGELADPSKSLYTVADLSSVWVLVDINEKDLAKVHKGQGAVVTVGAFPDLKL
HHCCCHHHHCCCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEE
KGRITYIADLVDESTRTVKARVEVTNPGRKLKPEMFATAELALAADAPPVLAVPEDAVQD
CCEEEEEHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHEEEEEECCCCCEEECCHHHHHC
LDGKKVVFVAEEGNRFEARLVQLGRSAAGLVEIASGLKEGENYAAKGAFILKSEVKKGEV
CCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCE
VDEHGHGK
ECCCCCCC
>Mature Secondary Structure 
SKKAKIVGLILALALTGAFTYRFVQMGAAGSEPGEHAGEAGHTEAKHEEEGHAKEEKGH
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCCCCCHHCCCHHHCCC
EEEHAGVAGHDEHGAAGSVTMKPEIQKQNGVVLAAAKPLRLAGVIRATGKVEVNADRIAH
CHHHCCCCCCCCCCCCCEEEECCCCCCCCCEEEEECCCEEEEEEEEECCEEEECHHHHHH
VAPRIPGKVVSVRASLGDSVSAGQSLATLDSVEVGEAIGRYRQAKTRLALAQGNMDRVKA
HCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
LVDRKIAARKDILQAETDYKTAQTELATDEERLSLYGVSVAEVRGGARKPLLPVRSPIGG
HHHHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHEEECCCHHHHCCCCCCCCCCCCCCCCC
IVTEKHAIVGELADPSKSLYTVADLSSVWVLVDINEKDLAKVHKGQGAVVTVGAFPDLKL
HHCCCHHHHCCCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHCCCCCEEEEECCCCEEE
KGRITYIADLVDESTRTVKARVEVTNPGRKLKPEMFATAELALAADAPPVLAVPEDAVQD
CCEEEEEHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHEEEEEECCCCCEEECCHHHHHC
LDGKKVVFVAEEGNRFEARLVQLGRSAAGLVEIASGLKEGENYAAKGAFILKSEVKKGEV
CCCCEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCCCE
VDEHGHGK
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]