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The map label for this gene is braC [H]

Identifier: 197118317

GI number: 197118317

Start: 2235574

End: 2236767

Strand: Direct

Name: braC [H]

Synonym: Gbem_1934

Alternate gene names: 197118317

Gene position: 2235574-2236767 (Clockwise)

Preceding gene: 197118316

Following gene: 197118318

Centisome position: 48.44

GC content: 60.64

Gene sequence:

>1194_bases
ATGAGCTTTAAGAAGATCAGCGCATTGCTCTTGGTAGCGATGCTGGCAGCCGGCGCATTCGGCTGCAAGAAGAAGGAAGA
GGCCGCAGCCCCGGCTGCAGGAGCCGCCAAACCGGCAGGCAACACCGTGAAGATCGGTTTCCTCGGCGCTCTCACCGGCG
ACGTGGCGATGTTCGGCAAGCCGACCCTCGAAGGTATGAAGATGGCTGCTGCTGAAATCAACGCAGCCGGCGGCATTCTC
GGCAAGCAGATCGAGATCGTCGAGGCCGACAACCGCGGCGACAAGCAGGAAGGCGCTTCGGTCACCCAGAAATTCATCTC
CCGCGACAACGTGACCGCCATCGTCGGCGACCCGACCACCGGCATCACCAAGGTTGCCGCTCCCATCGCGCAGAAGGCAG
GCGTGGTGCTGCTGTCCGCCGGCGCCACCGGCCCGGGCGTGGTCGAAGTGGGCGATTACATCTTCCGCGACACCCTGCTC
GACTCCATCGCTATTCCGGCCTGCATCCAGTACTTCGCGAAGGACCTCGGCTTCAAGAAGGTCGCCATCATCACTTCCGA
CAACAACGACTACTCGGTCGGCCTTTCCCAGACCTTCCGCGATGCCGCTGCCAAGGTTCCCTCGATCAAGATCGTAGCCG
ACGAGAAGGTGAAGGACGGCGACAAGGACTTCTCCGCTCAGATCACCAACATCAAGGCTAAGAAACCGGACGTCATCCTC
TTCTCCGGTTACTACACCGAGGGCGCTCTGATCATGAAAGAGGCGCGCAAGCAGGGTCTCAAGTCCCCGATGTTCGGCGG
CGACGGACTCTTCTCGCCTAAATTCATCGAGCTTGGCGGCCCCGCAGTCGAGGGCTCCATGTCCGCTCTGGGCTTCTCCA
CCGAGCAGGCTTCCCCTGCGACCGCGAAATTCATCGAGGCGTTCAAGGCGAAGCATAACGGCGAACTCCCGGGCCTCTTC
GACGCCCAGGGCTACGACGCAGTGATGCTCCTGGCAGACTCCATGAAGCGCGCCAACAGCGTAGACGCCAAGGTATTCAA
GGATGCCCTTGCCAAGACCAAAGGCTTCGAAGGCGTTTCCGGCACCATCAGCATGCAGGCCAACCGCGAGCCGATCAAGA
GCCCGCTTTCCCTTCTCTTTGTGAAGGACGGCAAGTTCGTGCTGAAGGCAAAAGTCCCCGTCAAAATGGACTAA

Upstream 100 bases:

>100_bases
GTCTGTGTCTGTCTTGACAATCAAGTAGTGACAATTAGAATCAGTTGACGTGTGAATTTTGTCTTTACAATGAGAACGAC
ATCCAAAGGAGGTAGTCTGC

Downstream 100 bases:

>100_bases
GGCCTGAACCCCGCAGTACCAGCGGCCCCTGTTTCGACAGGGGCCGTTTTTTTTTTACTCAGACAGGTCAGACAGATTTC
TCAGTGACTGACCGTTTACC

Product: branched-chain amino acid ABC transporter periplasmic amino acid-binding lipoprotein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: LIVAT-BP; Leu/Ile/Val/Thr/Ala-binding protein [H]

Number of amino acids: Translated: 397; Mature: 396

Protein sequence:

>397_residues
MSFKKISALLLVAMLAAGAFGCKKKEEAAAPAAGAAKPAGNTVKIGFLGALTGDVAMFGKPTLEGMKMAAAEINAAGGIL
GKQIEIVEADNRGDKQEGASVTQKFISRDNVTAIVGDPTTGITKVAAPIAQKAGVVLLSAGATGPGVVEVGDYIFRDTLL
DSIAIPACIQYFAKDLGFKKVAIITSDNNDYSVGLSQTFRDAAAKVPSIKIVADEKVKDGDKDFSAQITNIKAKKPDVIL
FSGYYTEGALIMKEARKQGLKSPMFGGDGLFSPKFIELGGPAVEGSMSALGFSTEQASPATAKFIEAFKAKHNGELPGLF
DAQGYDAVMLLADSMKRANSVDAKVFKDALAKTKGFEGVSGTISMQANREPIKSPLSLLFVKDGKFVLKAKVPVKMD

Sequences:

>Translated_397_residues
MSFKKISALLLVAMLAAGAFGCKKKEEAAAPAAGAAKPAGNTVKIGFLGALTGDVAMFGKPTLEGMKMAAAEINAAGGIL
GKQIEIVEADNRGDKQEGASVTQKFISRDNVTAIVGDPTTGITKVAAPIAQKAGVVLLSAGATGPGVVEVGDYIFRDTLL
DSIAIPACIQYFAKDLGFKKVAIITSDNNDYSVGLSQTFRDAAAKVPSIKIVADEKVKDGDKDFSAQITNIKAKKPDVIL
FSGYYTEGALIMKEARKQGLKSPMFGGDGLFSPKFIELGGPAVEGSMSALGFSTEQASPATAKFIEAFKAKHNGELPGLF
DAQGYDAVMLLADSMKRANSVDAKVFKDALAKTKGFEGVSGTISMQANREPIKSPLSLLFVKDGKFVLKAKVPVKMD
>Mature_396_residues
SFKKISALLLVAMLAAGAFGCKKKEEAAAPAAGAAKPAGNTVKIGFLGALTGDVAMFGKPTLEGMKMAAAEINAAGGILG
KQIEIVEADNRGDKQEGASVTQKFISRDNVTAIVGDPTTGITKVAAPIAQKAGVVLLSAGATGPGVVEVGDYIFRDTLLD
SIAIPACIQYFAKDLGFKKVAIITSDNNDYSVGLSQTFRDAAAKVPSIKIVADEKVKDGDKDFSAQITNIKAKKPDVILF
SGYYTEGALIMKEARKQGLKSPMFGGDGLFSPKFIELGGPAVEGSMSALGFSTEQASPATAKFIEAFKAKHNGELPGLFD
AQGYDAVMLLADSMKRANSVDAKVFKDALAKTKGFEGVSGTISMQANREPIKSPLSLLFVKDGKFVLKAKVPVKMD

Specific function: Component of the high-affinity leucine, isoleucine, valine transport system I (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=301, Percent_Identity=29.2358803986711, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI48994941, Length=342, Percent_Identity=28.0701754385965, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 41460; Mature: 41329

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFKKISALLLVAMLAAGAFGCKKKEEAAAPAAGAAKPAGNTVKIGFLGALTGDVAMFGK
CCHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCC
PTLEGMKMAAAEINAAGGILGKQIEIVEADNRGDKQEGASVTQKFISRDNVTAIVGDPTT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GITKVAAPIAQKAGVVLLSAGATGPGVVEVGDYIFRDTLLDSIAIPACIQYFAKDLGFKK
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VAIITSDNNDYSVGLSQTFRDAAAKVPSIKIVADEKVKDGDKDFSAQITNIKAKKPDVIL
EEEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEE
FSGYYTEGALIMKEARKQGLKSPMFGGDGLFSPKFIELGGPAVEGSMSALGFSTEQASPA
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCH
TAKFIEAFKAKHNGELPGLFDAQGYDAVMLLADSMKRANSVDAKVFKDALAKTKGFEGVS
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
GTISMQANREPIKSPLSLLFVKDGKFVLKAKVPVKMD
EEEEECCCCCCCCCCEEEEEEECCCEEEEEECCEECC
>Mature Secondary Structure 
SFKKISALLLVAMLAAGAFGCKKKEEAAAPAAGAAKPAGNTVKIGFLGALTGDVAMFGK
CHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCC
PTLEGMKMAAAEINAAGGILGKQIEIVEADNRGDKQEGASVTQKFISRDNVTAIVGDPTT
CCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
GITKVAAPIAQKAGVVLLSAGATGPGVVEVGDYIFRDTLLDSIAIPACIQYFAKDLGFKK
CHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
VAIITSDNNDYSVGLSQTFRDAAAKVPSIKIVADEKVKDGDKDFSAQITNIKAKKPDVIL
EEEEEECCCCEEECHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCEEEEEEECCCCCEEE
FSGYYTEGALIMKEARKQGLKSPMFGGDGLFSPKFIELGGPAVEGSMSALGFSTEQASPA
EECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCHHHCCCCCCCCCCH
TAKFIEAFKAKHNGELPGLFDAQGYDAVMLLADSMKRANSVDAKVFKDALAKTKGFEGVS
HHHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
GTISMQANREPIKSPLSLLFVKDGKFVLKAKVPVKMD
EEEEECCCCCCCCCCEEEEEEECCCEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2509433; 2120183; 10984043 [H]