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The map label for this gene is lepA

Identifier: 197118266

GI number: 197118266

Start: 2180382

End: 2182181

Strand: Reverse

Name: lepA

Synonym: Gbem_1883

Alternate gene names: 197118266

Gene position: 2182181-2180382 (Counterclockwise)

Preceding gene: 197118267

Following gene: 197118265

Centisome position: 47.28

GC content: 61.0

Gene sequence:

>1800_bases
ATGGTAATCGAGCATATCCGCAACTTTTCCATCATCGCCCACATCGACCATGGCAAATCCACTCTGGCAGACCGCCTACT
CGAATTCACCGGAACCGTTTCGGCCCGGGAAAAGCAGGACCAGTTCCTGGACAAAATGGACCTGGAACGCGAACGCGGCA
TCACCATCAAAGCACAGACCGTGCGACTCAACTACCGCGCCGACGACGGCAAAGACTATATCCTCAACCTGATCGACACC
CCGGGCCACGTCGACTTCACTTACGAGGTCTCCCGCTCGCTCACCGCCTGCGAAGGGGGCCTGCTGGTGGTCGACGCCTC
GCAGGGGGTGGAAGCCCAGACTTTAGCCAACGTCTACTTAGCCCTCGACGCCAACCTCGAGGTGTTCGTGGTGCTGAACA
AGATCGATCTCCCCGCGGCCGAGCCGGAACGGGTCAAGGCCGAGATCGAGGAGATCATCGGCCTCGACACCCACGACGCG
GTGCTGGCCAGCGCCAAGGAAGGGATCGGGACCAAGGACATCCTGGAAGAGATCGTCAAGAAGATCCCCCCACCCAAGGG
CGACGCGACCAAGCCCATGAAGGCGCTCCTTTTCGACTCGTGGTACGACCAGTACCAGGGGGTCATCATCCTGGTCCGCC
TGGTGGACGGGACGGTGAAGAAGGGTGACAAGATCCAGCTCATGTCGAACCGCAAGAGCTACGAGGTGCTTAAGGCCGGC
GTCTTCTCCCCGGAAATGCGGGAGACCCCGATGCTTTCCGCCGGCGAGGTCGGGTTTATCATCGCCGGGATCCGCGAGGT
GGCCGACGCAAAGGTAGGCGACACGGTGACGCTGTTGCACAACCCCTGCGACGCCGCCCTGCCGGGTTACAAGGAGGTGA
AGCCGATGGTCTTCTCCGGCCTTTACCCCATCGACACCTCGCAGTACGAGCAGTTGCGCGACGCGCTGGCCAAGCTGAAA
CTTAACGACTCCTCGTTCTCCTACGACCCCGAGACCTCGCTCGCGCTGGGCTTCGGCTTCCGCTGCGGCTTTCTCGGGCT
TTTGCACATGGAGATCATCCAGGAACGCCTGGAGCGCGAGTTCAACCTCGAACTGATCACCACGGCGCCCACCGTCGTCT
ACCGCGTACACGGCACCGACGGCAGCCTCACCTCGATCCAGAGCGCGAACCAGCTCCCGCCGACCCAGGAGATCGCCTAC
GTTGAAGAGCCGTTCATCCTCGCCTCGATCCACGTCCCCAACGACTTCGTGGGGGGGATCCTGGCCCTTTGCGAGGAGAA
GCGCGGCGTGCAGCGGGAAATCAAGTACCTGACCCCTACCCGCGTCATGGTGGTCTACGAGCTCCCCTTGAACGAGGTGG
TGCTCGACTTCTACGACCGGCTGAAATCGATCACCAAGGGGTACGCCTCGCTGGACTACGAGCTTCTGGACTACCGCCAG
AGCGAACTGGTGCGCCTCAACATCATGATCAACGGCGAGGTGGTGGACGCGCTGTCGCTTATCATCCACAAGGACAAGGC
GTACTACCGCGGTAGGGAACTGGTCTCCAAGATGAAGGAACTGATACCGCGCCAGATGTTCGAGATCGCCATCCAGGCCG
CGGTAGGGACCAAGGTCATCGCGCGCGAAACGGTCAAGGCGATGAGAAAGGACGTCCTCGCCAAGTGCTACGGCGGCGAC
ATCACCAGGAAGCGGAAACTCCTGGAGAAACAGAAAGAAGGCAAAAAGCGCATGAAGAACGTCGGCAATGTGGAGCTCCC
GCAGGAGGCGTTCCTGGCTATCCTCAAGGTAGAGGGGTAG

Upstream 100 bases:

>100_bases
CAGCGCCCTTTCCCTTAGGTTAAAGCCCCAGAAATCACTTTACAATTAACAACCCCTTCTATATATTTCATGAGTTAACC
TCCGCGAGCTAAAGGACAAT

Downstream 100 bases:

>100_bases
CCCCAACTCGGACGGCTTACGCCTTTCCATCAAGACAAGGGGATCAAAGGGAATGGAAGATTACAAAAACGTCTGCGAAG
ACAACAAGCCTGCGCAGTCT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 599; Mature: 599

Protein sequence:

>599_residues
MVIEHIRNFSIIAHIDHGKSTLADRLLEFTGTVSAREKQDQFLDKMDLERERGITIKAQTVRLNYRADDGKDYILNLIDT
PGHVDFTYEVSRSLTACEGGLLVVDASQGVEAQTLANVYLALDANLEVFVVLNKIDLPAAEPERVKAEIEEIIGLDTHDA
VLASAKEGIGTKDILEEIVKKIPPPKGDATKPMKALLFDSWYDQYQGVIILVRLVDGTVKKGDKIQLMSNRKSYEVLKAG
VFSPEMRETPMLSAGEVGFIIAGIREVADAKVGDTVTLLHNPCDAALPGYKEVKPMVFSGLYPIDTSQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLELITTAPTVVYRVHGTDGSLTSIQSANQLPPTQEIAY
VEEPFILASIHVPNDFVGGILALCEEKRGVQREIKYLTPTRVMVVYELPLNEVVLDFYDRLKSITKGYASLDYELLDYRQ
SELVRLNIMINGEVVDALSLIIHKDKAYYRGRELVSKMKELIPRQMFEIAIQAAVGTKVIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEG

Sequences:

>Translated_599_residues
MVIEHIRNFSIIAHIDHGKSTLADRLLEFTGTVSAREKQDQFLDKMDLERERGITIKAQTVRLNYRADDGKDYILNLIDT
PGHVDFTYEVSRSLTACEGGLLVVDASQGVEAQTLANVYLALDANLEVFVVLNKIDLPAAEPERVKAEIEEIIGLDTHDA
VLASAKEGIGTKDILEEIVKKIPPPKGDATKPMKALLFDSWYDQYQGVIILVRLVDGTVKKGDKIQLMSNRKSYEVLKAG
VFSPEMRETPMLSAGEVGFIIAGIREVADAKVGDTVTLLHNPCDAALPGYKEVKPMVFSGLYPIDTSQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLELITTAPTVVYRVHGTDGSLTSIQSANQLPPTQEIAY
VEEPFILASIHVPNDFVGGILALCEEKRGVQREIKYLTPTRVMVVYELPLNEVVLDFYDRLKSITKGYASLDYELLDYRQ
SELVRLNIMINGEVVDALSLIIHKDKAYYRGRELVSKMKELIPRQMFEIAIQAAVGTKVIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEG
>Mature_599_residues
MVIEHIRNFSIIAHIDHGKSTLADRLLEFTGTVSAREKQDQFLDKMDLERERGITIKAQTVRLNYRADDGKDYILNLIDT
PGHVDFTYEVSRSLTACEGGLLVVDASQGVEAQTLANVYLALDANLEVFVVLNKIDLPAAEPERVKAEIEEIIGLDTHDA
VLASAKEGIGTKDILEEIVKKIPPPKGDATKPMKALLFDSWYDQYQGVIILVRLVDGTVKKGDKIQLMSNRKSYEVLKAG
VFSPEMRETPMLSAGEVGFIIAGIREVADAKVGDTVTLLHNPCDAALPGYKEVKPMVFSGLYPIDTSQYEQLRDALAKLK
LNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLEREFNLELITTAPTVVYRVHGTDGSLTSIQSANQLPPTQEIAY
VEEPFILASIHVPNDFVGGILALCEEKRGVQREIKYLTPTRVMVVYELPLNEVVLDFYDRLKSITKGYASLDYELLDYRQ
SELVRLNIMINGEVVDALSLIIHKDKAYYRGRELVSKMKELIPRQMFEIAIQAAVGTKVIARETVKAMRKDVLAKCYGGD
ITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEG

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=605, Percent_Identity=51.7355371900826, Blast_Score=660, Evalue=0.0,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=44.3609022556391, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI4503483, Length=144, Percent_Identity=40.9722222222222, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI18390331, Length=185, Percent_Identity=35.1351351351351, Blast_Score=107, Evalue=5e-23,
Organism=Homo sapiens, GI25306283, Length=137, Percent_Identity=46.7153284671533, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI25306287, Length=137, Percent_Identity=45.985401459854, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI19923640, Length=137, Percent_Identity=46.7153284671533, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=41.8181818181818, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=41.8181818181818, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=41.8181818181818, Blast_Score=97, Evalue=6e-20,
Organism=Homo sapiens, GI217272894, Length=136, Percent_Identity=38.9705882352941, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI217272892, Length=136, Percent_Identity=38.9705882352941, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI34147630, Length=255, Percent_Identity=29.8039215686275, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI94966752, Length=94, Percent_Identity=41.4893617021277, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI1788922, Length=595, Percent_Identity=60.3361344537815, Blast_Score=729, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=507, Percent_Identity=30.1775147928994, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1790835, Length=151, Percent_Identity=34.4370860927152, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1789738, Length=211, Percent_Identity=33.6492890995261, Blast_Score=86, Evalue=9e-18,
Organism=Escherichia coli, GI1789559, Length=253, Percent_Identity=29.2490118577075, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=42.483660130719, Blast_Score=500, Evalue=1e-141,
Organism=Caenorhabditis elegans, GI17556745, Length=153, Percent_Identity=37.9084967320261, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17533571, Length=146, Percent_Identity=39.7260273972603, Blast_Score=102, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17506493, Length=156, Percent_Identity=35.8974358974359, Blast_Score=96, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI71988811, Length=134, Percent_Identity=36.5671641791045, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988819, Length=134, Percent_Identity=36.5671641791045, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=139, Percent_Identity=37.410071942446, Blast_Score=92, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=255, Percent_Identity=29.8039215686275, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=46.843853820598, Blast_Score=561, Evalue=1e-160,
Organism=Saccharomyces cerevisiae, GI6324707, Length=146, Percent_Identity=41.0958904109589, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=146, Percent_Identity=41.0958904109589, Blast_Score=112, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=145, Percent_Identity=40.6896551724138, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=40.8695652173913, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=141, Percent_Identity=39.7163120567376, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324761, Length=284, Percent_Identity=28.5211267605634, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI78706572, Length=604, Percent_Identity=46.8543046357616, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24582462, Length=173, Percent_Identity=36.9942196531792, Blast_Score=108, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574573, Length=216, Percent_Identity=35.6481481481481, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585711, Length=148, Percent_Identity=37.1621621621622, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=148, Percent_Identity=37.1621621621622, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585709, Length=148, Percent_Identity=37.1621621621622, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI221458488, Length=149, Percent_Identity=38.9261744966443, Blast_Score=96, Evalue=9e-20,
Organism=Drosophila melanogaster, GI21357743, Length=160, Percent_Identity=34.375, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI28572034, Length=222, Percent_Identity=30.6306306306306, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI281363316, Length=259, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17864358, Length=259, Percent_Identity=28.5714285714286, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921738, Length=280, Percent_Identity=26.7857142857143, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_GEOBB (B5EB36)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138693.1
- GeneID:   6781873
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1883
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 67013; Mature: 67013

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIEHIRNFSIIAHIDHGKSTLADRLLEFTGTVSAREKQDQFLDKMDLERERGITIKAQT
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLNYRADDGKDYILNLIDTPGHVDFTYEVSRSLTACEGGLLVVDASQGVEAQTLANVYL
EEEEEECCCCCEEEEEEECCCCCEEEEEECCCCHHCCCCCEEEEECCCCCCHHHHEEEEE
ALDANLEVFVVLNKIDLPAAEPERVKAEIEEIIGLDTHDAVLASAKEGIGTKDILEEIVK
EEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIPPPKGDATKPMKALLFDSWYDQYQGVIILVRLVDGTVKKGDKIQLMSNRKSYEVLKAG
HCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHC
VFSPEMRETPMLSAGEVGFIIAGIREVADAKVGDTVTLLHNPCDAALPGYKEVKPMVFSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCHHHHCC
LYPIDTSQYEQLRDALAKLKLNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNLELITTAPTVVYRVHGTDGSLTSIQSANQLPPTQEIAYVEEPFILASIHVPNDFVGGI
CCEEEEEECCEEEEEEECCCCCCHHHCCCCCCCCCHHHHEECCCEEEEEEECCCHHHHHH
LALCEEKRGVQREIKYLTPTRVMVVYELPLNEVVLDFYDRLKSITKGYASLDYELLDYRQ
HHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
SELVRLNIMINGEVVDALSLIIHKDKAYYRGRELVSKMKELIPRQMFEIAIQAAVGTKVI
CCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ARETVKAMRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEECC
>Mature Secondary Structure
MVIEHIRNFSIIAHIDHGKSTLADRLLEFTGTVSAREKQDQFLDKMDLERERGITIKAQT
CCCHHCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHCCCEEEEEE
VRLNYRADDGKDYILNLIDTPGHVDFTYEVSRSLTACEGGLLVVDASQGVEAQTLANVYL
EEEEEECCCCCEEEEEEECCCCCEEEEEECCCCHHCCCCCEEEEECCCCCCHHHHEEEEE
ALDANLEVFVVLNKIDLPAAEPERVKAEIEEIIGLDTHDAVLASAKEGIGTKDILEEIVK
EEECCCEEEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHH
KIPPPKGDATKPMKALLFDSWYDQYQGVIILVRLVDGTVKKGDKIQLMSNRKSYEVLKAG
HCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCEEEEECCCCHHHHHHHC
VFSPEMRETPMLSAGEVGFIIAGIREVADAKVGDTVTLLHNPCDAALPGYKEVKPMVFSG
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHCCHHHHCC
LYPIDTSQYEQLRDALAKLKLNDSSFSYDPETSLALGFGFRCGFLGLLHMEIIQERLERE
CCCCCCHHHHHHHHHHHHEEECCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHH
FNLELITTAPTVVYRVHGTDGSLTSIQSANQLPPTQEIAYVEEPFILASIHVPNDFVGGI
CCEEEEEECCEEEEEEECCCCCCHHHCCCCCCCCCHHHHEECCCEEEEEEECCCHHHHHH
LALCEEKRGVQREIKYLTPTRVMVVYELPLNEVVLDFYDRLKSITKGYASLDYELLDYRQ
HHHHHHHCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
SELVRLNIMINGEVVDALSLIIHKDKAYYRGRELVSKMKELIPRQMFEIAIQAAVGTKVI
CCEEEEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
ARETVKAMRKDVLAKCYGGDITRKRKLLEKQKEGKKRMKNVGNVELPQEAFLAILKVEG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA