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The map label for this gene is yjbJ [H]

Identifier: 197118256

GI number: 197118256

Start: 2167600

End: 2168214

Strand: Reverse

Name: yjbJ [H]

Synonym: Gbem_1873

Alternate gene names: 197118256

Gene position: 2168214-2167600 (Counterclockwise)

Preceding gene: 197118264

Following gene: 197118255

Centisome position: 46.98

GC content: 60.49

Gene sequence:

>615_bases
ATGCCCTGTAGGTCTCAACGACTGCTTTTTGCGGGGTTGGCGACGCTGCTGGCGCTCTCCAGCGCCCCCCTCGCCCCGGC
CCGTGCCGACATATACCGTTACGAGGATCCCGACGGGACCCTGCACTTCACCGATGCACCTACCGACAAGCGGTTCAAGG
TGTTCATGAGGGACATAAAGAAGGACAAGAAACTAAGGACCGCCTTCAAGCTCCCCGGCTACGCCAGGAACCCCGCAGAA
TTCGAGCCCATAATCAACTCCTGCTCACGCGAGTTCGGCGTCGACAGCTCGCTGGTCAAAGCCGTAATCCATGCCGAGTC
CGGCTACAACCCCTCCGCCGTCTCGTCCAAGGGGGCCCAAGGGCTCATGCAGCTGATGCCCAAGACCGCCCAGGGGCTCA
AGGTCTCGGATTGCTTCAACCCCTCCGACAACATCCGCGGCGGGGTACGCTACCTGAGGTTCCTTCTGGACACCTTCAAG
GGAGACGAGTCGCTGGCTTTGGCCGCCTACAACTCGGGCATGGGGTCCGTCGCCAAGTACGGCGGCATCCCCCCCTTCAA
GGAAACGCAGAGTTACGTGGCCAAAGTATTGAGTTACCGGAACAACTACAGATAG

Upstream 100 bases:

>100_bases
GGTTATCGCCCCTTCGGTTCACATTAGACCTTGAATTATCATGCTTATTGGTCTATATTTCCGGTGGCTGATTTCTCTCA
CCCATTAAAAAAGGAAGCTC

Downstream 100 bases:

>100_bases
CGCAAAGGCATACAGCATGTCCTCCCCCCCCCCAGCGAAGATAATGAACATCCCCAACATCCTCACCATGGTGCGGATCG
CGGCGATACCGATTCTGTGC

Product: lytic transglycosylase domain-containing protein

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 204; Mature: 203

Protein sequence:

>204_residues
MPCRSQRLLFAGLATLLALSSAPLAPARADIYRYEDPDGTLHFTDAPTDKRFKVFMRDIKKDKKLRTAFKLPGYARNPAE
FEPIINSCSREFGVDSSLVKAVIHAESGYNPSAVSSKGAQGLMQLMPKTAQGLKVSDCFNPSDNIRGGVRYLRFLLDTFK
GDESLALAAYNSGMGSVAKYGGIPPFKETQSYVAKVLSYRNNYR

Sequences:

>Translated_204_residues
MPCRSQRLLFAGLATLLALSSAPLAPARADIYRYEDPDGTLHFTDAPTDKRFKVFMRDIKKDKKLRTAFKLPGYARNPAE
FEPIINSCSREFGVDSSLVKAVIHAESGYNPSAVSSKGAQGLMQLMPKTAQGLKVSDCFNPSDNIRGGVRYLRFLLDTFK
GDESLALAAYNSGMGSVAKYGGIPPFKETQSYVAKVLSYRNNYR
>Mature_203_residues
PCRSQRLLFAGLATLLALSSAPLAPARADIYRYEDPDGTLHFTDAPTDKRFKVFMRDIKKDKKLRTAFKLPGYARNPAEF
EPIINSCSREFGVDSSLVKAVIHAESGYNPSAVSSKGAQGLMQLMPKTAQGLKVSDCFNPSDNIRGGVRYLRFLLDTFKG
DESLALAAYNSGMGSVAKYGGIPPFKETQSYVAKVLSYRNNYR

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=152, Percent_Identity=30.2631578947368, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI87082191, Length=114, Percent_Identity=35.9649122807018, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 22490; Mature: 22359

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCRSQRLLFAGLATLLALSSAPLAPARADIYRYEDPDGTLHFTDAPTDKRFKVFMRDIK
CCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECCCCEEEECCCCCCHHHHHHHHHHH
KDKKLRTAFKLPGYARNPAEFEPIINSCSREFGVDSSLVKAVIHAESGYNPSAVSSKGAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCCHHHH
GLMQLMPKTAQGLKVSDCFNPSDNIRGGVRYLRFLLDTFKGDESLALAAYNSGMGSVAKY
HHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
GGIPPFKETQSYVAKVLSYRNNYR
CCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PCRSQRLLFAGLATLLALSSAPLAPARADIYRYEDPDGTLHFTDAPTDKRFKVFMRDIK
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHEEEECCCCEEEECCCCCCHHHHHHHHHHH
KDKKLRTAFKLPGYARNPAEFEPIINSCSREFGVDSSLVKAVIHAESGYNPSAVSSKGAQ
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHCCHHHH
GLMQLMPKTAQGLKVSDCFNPSDNIRGGVRYLRFLLDTFKGDESLALAAYNSGMGSVAKY
HHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHH
GGIPPFKETQSYVAKVLSYRNNYR
CCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]