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The map label for this gene is zraS [H]

Identifier: 197118049

GI number: 197118049

Start: 1928127

End: 1929317

Strand: Direct

Name: zraS [H]

Synonym: Gbem_1662

Alternate gene names: 197118049

Gene position: 1928127-1929317 (Clockwise)

Preceding gene: 197118048

Following gene: 197118050

Centisome position: 41.78

GC content: 61.21

Gene sequence:

>1191_bases
ATGTCCCAACAGGATAAAGAGCCGCGCGGCCGCGTGCTGATAGTCGACGACGAGAAGGTCATCCTCGACCTTACCGCCAT
CATCCTCAAAAACCGCGGCTACCAGGTCTTCACCGCACTTTCGGCGCTGGAGGGGCTGGAGGTCATCGAGAAGGAACGCC
CCGAGCTCGTGCTTCTCGATTACATGATGCCGAACATGGACGGCCTCACCGCGCTCAAGGAGATCAGGCGCAGCTACCCC
GATACCTACGTGATCATGTTCACCGGCAAGGGGAGCGAGGAGATCGCCGTCGAGCTGATGAAGGCGGGGGCCTCCGACTA
CATACTGAAGCCGTTCAACAACCAGGACCTGGTCGAGAGGATCGAGAGCGTCCTGAAGCTGCGGGGGATCGAGCTGCAAA
ACCGCGCCCTTTTGAGCGAGCGGGAAAGGCTTCTGGCCGAGATCGCGGACTGGAACCGCGAGCTGGAGCGCCGGGTGCAG
GAAAAGAGCGAGGCGCTGCGCCGGGCCCAGGCAGAAGTGGTGCAGTCCGAGAAGCTCGCTTCCCTTGGCTACCTCTCCGC
CGGGATGGCGCACGAGATCAGGAACCCCTTGAACTCCATAGCGCTTTTCGTGCAGCTGATCAAAACCGGGCTGGACGAGC
ACGAGCGCCTGGATTACGTGGAAAAGATCCTCAAAGAAGTCGACCGGATCGACAACATCCTGGCCAAGCTACTCGACGCC
TCCAAGCGGCCGAAGTTCGAGATCAGCGAGGTGAGGGTGGACCGGGTCCTGGAGCACACGCTCGACGCCTTCACCCCGCA
GCTGCGGCAGAAAAGGATCCGGGCGGTCACCGATTTCAAGAGCATCCCGCCTGCCATCAAGGCGGACCCGATGGAGATAG
AGCAGATCTTCACCAACCTCTTTTTGAACTCCATCTACGTGATGCCTGAGGAGGGGACCCTCGCGGTGGAGCTGGAAGGG
GACGAGCAGTGGATCACTGTGAGGGTCTCCGATACCGGCCCCGGCATCCCGCCTGAAAACCTCCCCAACATCTTCGACCC
CTTCTTCACCACCAACAGCCGCGGCACCGGGCTTGGGCTCTCGGTGGTCCTGCGCATCGTGAAGACCTACAAGGGGAAGA
TCGAAGTGGAGAAAAGCGACAGCTTAGGGACCACCTTTCTGGTCCGTCTGCCGCTTGCCCCCCCGAGGTAG

Upstream 100 bases:

>100_bases
GGCTCGCCTGACTCCGGCGCCATAAGACTTCTGCGGGGCAGCGTTTTGCTGCCCCTTTTTTTTGATGCACCAAAATCACT
ACAGAACAGGAAAACCCGTC

Downstream 100 bases:

>100_bases
CGGGGGAGGATTCTCCCTTCCCTTTTGCAATACATTTTTGTATGATGGCGCATTCCATCGCGCTTTGAGGTTGAAATGAC
AGCAGAAAACCGGAAAAAGA

Product: response receiver histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSQQDKEPRGRVLIVDDEKVILDLTAIILKNRGYQVFTALSALEGLEVIEKERPELVLLDYMMPNMDGLTALKEIRRSYP
DTYVIMFTGKGSEEIAVELMKAGASDYILKPFNNQDLVERIESVLKLRGIELQNRALLSERERLLAEIADWNRELERRVQ
EKSEALRRAQAEVVQSEKLASLGYLSAGMAHEIRNPLNSIALFVQLIKTGLDEHERLDYVEKILKEVDRIDNILAKLLDA
SKRPKFEISEVRVDRVLEHTLDAFTPQLRQKRIRAVTDFKSIPPAIKADPMEIEQIFTNLFLNSIYVMPEEGTLAVELEG
DEQWITVRVSDTGPGIPPENLPNIFDPFFTTNSRGTGLGLSVVLRIVKTYKGKIEVEKSDSLGTTFLVRLPLAPPR

Sequences:

>Translated_396_residues
MSQQDKEPRGRVLIVDDEKVILDLTAIILKNRGYQVFTALSALEGLEVIEKERPELVLLDYMMPNMDGLTALKEIRRSYP
DTYVIMFTGKGSEEIAVELMKAGASDYILKPFNNQDLVERIESVLKLRGIELQNRALLSERERLLAEIADWNRELERRVQ
EKSEALRRAQAEVVQSEKLASLGYLSAGMAHEIRNPLNSIALFVQLIKTGLDEHERLDYVEKILKEVDRIDNILAKLLDA
SKRPKFEISEVRVDRVLEHTLDAFTPQLRQKRIRAVTDFKSIPPAIKADPMEIEQIFTNLFLNSIYVMPEEGTLAVELEG
DEQWITVRVSDTGPGIPPENLPNIFDPFFTTNSRGTGLGLSVVLRIVKTYKGKIEVEKSDSLGTTFLVRLPLAPPR
>Mature_395_residues
SQQDKEPRGRVLIVDDEKVILDLTAIILKNRGYQVFTALSALEGLEVIEKERPELVLLDYMMPNMDGLTALKEIRRSYPD
TYVIMFTGKGSEEIAVELMKAGASDYILKPFNNQDLVERIESVLKLRGIELQNRALLSERERLLAEIADWNRELERRVQE
KSEALRRAQAEVVQSEKLASLGYLSAGMAHEIRNPLNSIALFVQLIKTGLDEHERLDYVEKILKEVDRIDNILAKLLDAS
KRPKFEISEVRVDRVLEHTLDAFTPQLRQKRIRAVTDFKSIPPAIKADPMEIEQIFTNLFLNSIYVMPEEGTLAVELEGD
EQWITVRVSDTGPGIPPENLPNIFDPFFTTNSRGTGLGLSVVLRIVKTYKGKIEVEKSDSLGTTFLVRLPLAPPR

Specific function: Member of the two-component regulatory system zraS/zraR. May function as a membrane-associated protein kinase that phosphorylates zraR in response to high concentrations of zinc or lead in the medium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1790436, Length=227, Percent_Identity=33.920704845815, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI1788549, Length=231, Percent_Identity=31.1688311688312, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI87081816, Length=268, Percent_Identity=32.089552238806, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=28.3464566929134, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1790300, Length=237, Percent_Identity=29.535864978903, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1790346, Length=223, Percent_Identity=27.3542600896861, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788550, Length=127, Percent_Identity=34.6456692913386, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1786599, Length=145, Percent_Identity=32.4137931034483, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790861, Length=210, Percent_Identity=25.7142857142857, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1789149, Length=246, Percent_Identity=29.6747967479675, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788713, Length=248, Percent_Identity=24.1935483870968, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1790437, Length=114, Percent_Identity=36.8421052631579, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1789809, Length=122, Percent_Identity=34.4262295081967, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788394, Length=116, Percent_Identity=34.4827586206897, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1789808, Length=219, Percent_Identity=27.3972602739726, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790299, Length=118, Percent_Identity=33.8983050847458, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1788905, Length=117, Percent_Identity=35.042735042735, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87082128, Length=215, Percent_Identity=23.2558139534884, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787375, Length=116, Percent_Identity=26.7241379310345, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI2367329, Length=116, Percent_Identity=37.0689655172414, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994928, Length=211, Percent_Identity=27.0142180094787, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1786911, Length=115, Percent_Identity=31.304347826087, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1788393, Length=218, Percent_Identity=25.6880733944954, Blast_Score=62, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6322000, Length=107, Percent_Identity=33.6448598130841, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 44982; Mature: 44850

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQDKEPRGRVLIVDDEKVILDLTAIILKNRGYQVFTALSALEGLEVIEKERPELVLLD
CCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
YMMPNMDGLTALKEIRRSYPDTYVIMFTGKGSEEIAVELMKAGASDYILKPFNNQDLVER
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCHHHHHH
IESVLKLRGIELQNRALLSERERLLAEIADWNRELERRVQEKSEALRRAQAEVVQSEKLA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGYLSAGMAHEIRNPLNSIALFVQLIKTGLDEHERLDYVEKILKEVDRIDNILAKLLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SKRPKFEISEVRVDRVLEHTLDAFTPQLRQKRIRAVTDFKSIPPAIKADPMEIEQIFTNL
CCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FLNSIYVMPEEGTLAVELEGDEQWITVRVSDTGPGIPPENLPNIFDPFFTTNSRGTGLGL
HHHHEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCHHH
SVVLRIVKTYKGKIEVEKSDSLGTTFLVRLPLAPPR
HHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCC
>Mature Secondary Structure 
SQQDKEPRGRVLIVDDEKVILDLTAIILKNRGYQVFTALSALEGLEVIEKERPELVLLD
CCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
YMMPNMDGLTALKEIRRSYPDTYVIMFTGKGSEEIAVELMKAGASDYILKPFNNQDLVER
CCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCCEECCCCCHHHHHH
IESVLKLRGIELQNRALLSERERLLAEIADWNRELERRVQEKSEALRRAQAEVVQSEKLA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLGYLSAGMAHEIRNPLNSIALFVQLIKTGLDEHERLDYVEKILKEVDRIDNILAKLLDA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SKRPKFEISEVRVDRVLEHTLDAFTPQLRQKRIRAVTDFKSIPPAIKADPMEIEQIFTNL
CCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
FLNSIYVMPEEGTLAVELEGDEQWITVRVSDTGPGIPPENLPNIFDPFFTTNSRGTGLGL
HHHHEEEEECCCEEEEEEECCCEEEEEEECCCCCCCCCCCCCCHHCCCCCCCCCCCCHHH
SVVLRIVKTYKGKIEVEKSDSLGTTFLVRLPLAPPR
HHHHHHHHHHCCEEEEECCCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]