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The map label for this gene is rlmN [H]

Identifier: 197118042

GI number: 197118042

Start: 1922324

End: 1923379

Strand: Direct

Name: rlmN [H]

Synonym: Gbem_1655

Alternate gene names: 197118042

Gene position: 1922324-1923379 (Clockwise)

Preceding gene: 197118041

Following gene: 197118043

Centisome position: 41.65

GC content: 62.5

Gene sequence:

>1056_bases
ATGCAGAAAACAGACATAAAGAATTTGACCCTGCAGGGTCTCGAGTCGTACATCTCAGGGCTGGGCAAGGAGCGCTTCCG
CGCCAAGCAGATCTTCAAGTGGCTCTACCAGATGGACGCCGGAAGTTTCGAGGAGATGACCAACGTCTCCAAGGAGTTCC
GGGTCATGCTGGGCGAGATCGCCCAGATCAGCAACCTGACGCCCGAGGTGGTTGAGGCGTCGGAGGACGGGACCAGGAAG
TACCTCTTCCGCCTGTCCGACGGCAGCGCGGTGGAGAGCGTCCTGATTCCCGACGAGGGGCGCAACACGCTTTGCATCTC
GAGCCAGGTCGGCTGCGCCATGGGTTGCGCCTTCTGCCTCACCGGCAGCTTCGGGCTCTCCCGCAACCTCACCACGGCCG
AAATTGTGAACCAGGTCTGCGCGGTGAAGCGCGACCAGCCGGTCAATAACATCGTCTTCATGGGGATGGGGGAGCCGCTG
GCGAACCTGAAAGGGGTGATCCCGGCAGTGCAGATCCTGACCGACCCGGACGGCTTCCAGTTCTCCACCAGGAAGGTCAC
CGTTTCCACCTCCGGGCTCGTCCCCGAGATGGCGGAACTCGGGCGCGGCTGCACCGTGAACCTCGCCGTCTCGCTGAACG
CCACCACCGACGAGGTGAGGAGCCGGATCATGCCGGTGAACCGCACTTATCCTCTGGCCCAGTTGCTCGCCGCCTGCAAG
GCGTTCCCGCTTCCCTCCCGGCGCTGGATCACCATGGAGTACGTGATGATCCGGGACCTGAACGACTCGCTTGAAGACGC
GAAGAGGCTGGTGCGGCTGATCAGCAACATCCCCTCCAAGGTGAACCTGATCCCGTTCAACGAGCACGAAGGGTGCGACT
TCAAATGCCCCACCCAGGAGTCGATCGACCGCTTCCACAAGTATCTGTTGGACAAAAACGTCACCGTGATCACCCGTTCC
AGCCGCGGCAGCGACATCTCTGCCGCCTGCGGCCAGCTCAAGGGACGGCTGGATCAGGAGGACGGCGAACGCCGCAACTG
CGGCTGCGAAGGCTAG

Upstream 100 bases:

>100_bases
CCTTACTTCTTCAACCAGCTCGAGCTGGTGTAATTCGCAACTGGTCTAGGCAGATCGAAAGCCCTTCGCTAGAAGGGCTT
TTTTATATCCGAAAAAGGCG

Downstream 100 bases:

>100_bases
AGAGAACTGAAATAAAATCCCTGGAGGTAGAGATGGCAAACGAGGTAATCGGCGTTATCGGCGGCAGCGGGCTCTACGAG
ATGGAAGGGATGACCGGCGT

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MQKTDIKNLTLQGLESYISGLGKERFRAKQIFKWLYQMDAGSFEEMTNVSKEFRVMLGEIAQISNLTPEVVEASEDGTRK
YLFRLSDGSAVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGSFGLSRNLTTAEIVNQVCAVKRDQPVNNIVFMGMGEPL
ANLKGVIPAVQILTDPDGFQFSTRKVTVSTSGLVPEMAELGRGCTVNLAVSLNATTDEVRSRIMPVNRTYPLAQLLAACK
AFPLPSRRWITMEYVMIRDLNDSLEDAKRLVRLISNIPSKVNLIPFNEHEGCDFKCPTQESIDRFHKYLLDKNVTVITRS
SRGSDISAACGQLKGRLDQEDGERRNCGCEG

Sequences:

>Translated_351_residues
MQKTDIKNLTLQGLESYISGLGKERFRAKQIFKWLYQMDAGSFEEMTNVSKEFRVMLGEIAQISNLTPEVVEASEDGTRK
YLFRLSDGSAVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGSFGLSRNLTTAEIVNQVCAVKRDQPVNNIVFMGMGEPL
ANLKGVIPAVQILTDPDGFQFSTRKVTVSTSGLVPEMAELGRGCTVNLAVSLNATTDEVRSRIMPVNRTYPLAQLLAACK
AFPLPSRRWITMEYVMIRDLNDSLEDAKRLVRLISNIPSKVNLIPFNEHEGCDFKCPTQESIDRFHKYLLDKNVTVITRS
SRGSDISAACGQLKGRLDQEDGERRNCGCEG
>Mature_351_residues
MQKTDIKNLTLQGLESYISGLGKERFRAKQIFKWLYQMDAGSFEEMTNVSKEFRVMLGEIAQISNLTPEVVEASEDGTRK
YLFRLSDGSAVESVLIPDEGRNTLCISSQVGCAMGCAFCLTGSFGLSRNLTTAEIVNQVCAVKRDQPVNNIVFMGMGEPL
ANLKGVIPAVQILTDPDGFQFSTRKVTVSTSGLVPEMAELGRGCTVNLAVSLNATTDEVRSRIMPVNRTYPLAQLLAACK
AFPLPSRRWITMEYVMIRDLNDSLEDAKRLVRLISNIPSKVNLIPFNEHEGCDFKCPTQESIDRFHKYLLDKNVTVITRS
SRGSDISAACGQLKGRLDQEDGERRNCGCEG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=347, Percent_Identity=42.9394812680115, Blast_Score=280, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 38829; Mature: 38829

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKTDIKNLTLQGLESYISGLGKERFRAKQIFKWLYQMDAGSFEEMTNVSKEFRVMLGEI
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AQISNLTPEVVEASEDGTRKYLFRLSDGSAVESVLIPDEGRNTLCISSQVGCAMGCAFCL
HHHHCCCHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
TGSFGLSRNLTTAEIVNQVCAVKRDQPVNNIVFMGMGEPLANLKGVIPAVQILTDPDGFQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHEEECCCCCE
FSTRKVTVSTSGLVPEMAELGRGCTVNLAVSLNATTDEVRSRIMPVNRTYPLAQLLAACK
EEEEEEEEECCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHH
AFPLPSRRWITMEYVMIRDLNDSLEDAKRLVRLISNIPSKVNLIPFNEHEGCDFKCPTQE
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHH
SIDRFHKYLLDKNVTVITRSSRGSDISAACGQLKGRLDQEDGERRNCGCEG
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQKTDIKNLTLQGLESYISGLGKERFRAKQIFKWLYQMDAGSFEEMTNVSKEFRVMLGEI
CCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AQISNLTPEVVEASEDGTRKYLFRLSDGSAVESVLIPDEGRNTLCISSQVGCAMGCAFCL
HHHHCCCHHHHHCCCCCCEEEEEEECCCCCEEEEECCCCCCCEEEEECCCCHHHHHHHHH
TGSFGLSRNLTTAEIVNQVCAVKRDQPVNNIVFMGMGEPLANLKGVIPAVQILTDPDGFQ
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHEEECCCCCE
FSTRKVTVSTSGLVPEMAELGRGCTVNLAVSLNATTDEVRSRIMPVNRTYPLAQLLAACK
EEEEEEEEECCCCCHHHHHHCCCCEEEEEEEECCCHHHHHHHCCCCCCCCCHHHHHHHHH
AFPLPSRRWITMEYVMIRDLNDSLEDAKRLVRLISNIPSKVNLIPFNEHEGCDFKCPTQE
CCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHH
SIDRFHKYLLDKNVTVITRSSRGSDISAACGQLKGRLDQEDGERRNCGCEG
HHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA