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The map label for this gene is kdsA

Identifier: 197117988

GI number: 197117988

Start: 1856324

End: 1857145

Strand: Direct

Name: kdsA

Synonym: Gbem_1601

Alternate gene names: 197117988

Gene position: 1856324-1857145 (Clockwise)

Preceding gene: 197117987

Following gene: 197117989

Centisome position: 40.22

GC content: 62.65

Gene sequence:

>822_bases
ATGACGAGGGAAATCACCGTAGGTGGAGTGAAGATTGGCGGGGGAAGGCCGCTGGCGCTGGTAGCCGGGCCTTGCGTGAT
AGAAAACGAAACCGCCACGCTGCGCTGCGCCGAGAGGCTGATGAGCATCTGCAACGGCGTGGGGATCTCGCTGATCTTCA
AGGCCTCCTACGACAAGGCCAACCGTACTTCGGTCACCGCATTCCGCGGCCCCGGGATGAAGGAGGGGCTCAGGATCCTC
GCCAAGGTGAAAGAGGCGCTCGGCGTCCCGGTCCTTTCCGACATCCACTCCATCGAGCAGGTCGAGCCCGCGGCCGAAGT
CCTGGACGTGCTGCAGATCCCTGCCTTTCTCTGCCGCCAGACCGACCTCCTGGTCGCAGCCGGCAACACCGGGAAGGTGA
TCAACGTCAAGAAGGGGCAGTTCCTGGCCCCCTGGGACATGAAGAACGTGGTCGGCAAGATCAGCTCCTGCGACAACGAT
AACATCATCCTCACCGAGCGCGGCGCGAGCTTCGGTTACAACAACCTCGTCGTCGACATGAGGAGCTTCCCGATCATGCG
CTCCACCGGTTATCCGGTCATCTTCGATGCCACCCACAGCGTGCAGCTCCCGGGAGGGGAGGGGACCTCTTCCGGCGGAC
AGCGCGAGTACGTCGAGTTCCTCTCCCGCGCGGCTGTAGCGGCCGGGGTGGACGGCATCTTCATGGAAGTGCACGAGGAG
CCGGAGCAGGCCCTTTGCGACGGGCCTAACTCGGTGCGCCTGGACGACATGCCGGCGCTTTTGAAGAAGCTCAAAGCAAT
CGACGCTATCGTAAACCAGTAA

Upstream 100 bases:

>100_bases
GCAAAGGCTTAACAAGAGGTTTTCTTCTTGGCTCAGCCTTTATCCTGTTCATCCCCTTTATCCCTGTAGAAAGCCTTTAG
TTTTTTGATAAGGAGAGATC

Downstream 100 bases:

>100_bases
CCAAAGCATCTCACGCAAAGACGCAAAGGCGCAAAGAAAGGCAAAACAGAGGCTTAGGGGTAACCTGACCTTTTGTTTTG
CCTTTTGGGTCAAAGGCGCA

Product: 2-dehydro-3-deoxyphosphooctonate aldolase

Products: NA

Alternate protein names: 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase; KDO-8-phosphate synthase; KDO 8-P synthase; KDOPS; Phospho-2-dehydro-3-deoxyoctonate aldolase

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MTREITVGGVKIGGGRPLALVAGPCVIENETATLRCAERLMSICNGVGISLIFKASYDKANRTSVTAFRGPGMKEGLRIL
AKVKEALGVPVLSDIHSIEQVEPAAEVLDVLQIPAFLCRQTDLLVAAGNTGKVINVKKGQFLAPWDMKNVVGKISSCDND
NIILTERGASFGYNNLVVDMRSFPIMRSTGYPVIFDATHSVQLPGGEGTSSGGQREYVEFLSRAAVAAGVDGIFMEVHEE
PEQALCDGPNSVRLDDMPALLKKLKAIDAIVNQ

Sequences:

>Translated_273_residues
MTREITVGGVKIGGGRPLALVAGPCVIENETATLRCAERLMSICNGVGISLIFKASYDKANRTSVTAFRGPGMKEGLRIL
AKVKEALGVPVLSDIHSIEQVEPAAEVLDVLQIPAFLCRQTDLLVAAGNTGKVINVKKGQFLAPWDMKNVVGKISSCDND
NIILTERGASFGYNNLVVDMRSFPIMRSTGYPVIFDATHSVQLPGGEGTSSGGQREYVEFLSRAAVAAGVDGIFMEVHEE
PEQALCDGPNSVRLDDMPALLKKLKAIDAIVNQ
>Mature_272_residues
TREITVGGVKIGGGRPLALVAGPCVIENETATLRCAERLMSICNGVGISLIFKASYDKANRTSVTAFRGPGMKEGLRILA
KVKEALGVPVLSDIHSIEQVEPAAEVLDVLQIPAFLCRQTDLLVAAGNTGKVINVKKGQFLAPWDMKNVVGKISSCDNDN
IILTERGASFGYNNLVVDMRSFPIMRSTGYPVIFDATHSVQLPGGEGTSSGGQREYVEFLSRAAVAAGVDGIFMEVHEEP
EQALCDGPNSVRLDDMPALLKKLKAIDAIVNQ

Specific function: Synthesis Of Kdo 8-P Which Is Required For Lipid A Maturation And Cellular Growth. [C]

COG id: COG2877

COG function: function code M; 3-deoxy-D-manno-octulosonic acid (KDO) 8-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the kdsA family

Homologues:

Organism=Escherichia coli, GI1787466, Length=270, Percent_Identity=44.8148148148148, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): KDSA_GEOBB (B5E8N4)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138415.1
- ProteinModelPortal:   B5E8N4
- SMR:   B5E8N4
- GeneID:   6781589
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1601
- HOGENOM:   HBG575990
- OMA:   RSLPIMA
- ProtClustDB:   PRK05198
- GO:   GO:0005737
- HAMAP:   MF_00056
- InterPro:   IPR013785
- InterPro:   IPR006218
- InterPro:   IPR006269
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21057:SF2
- TIGRFAMs:   TIGR01362

Pfam domain/function: PF00793 DAHP_synth_1

EC number: =2.5.1.55

Molecular weight: Translated: 29172; Mature: 29041

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTREITVGGVKIGGGRPLALVAGPCVIENETATLRCAERLMSICNGVGISLIFKASYDKA
CCCEEEECCEEECCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
NRTSVTAFRGPGMKEGLRILAKVKEALGVPVLSDIHSIEQVEPAAEVLDVLQIPAFLCRQ
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
TDLLVAAGNTGKVINVKKGQFLAPWDMKNVVGKISSCDNDNIILTERGASFGYNNLVVDM
CCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEC
RSFPIMRSTGYPVIFDATHSVQLPGGEGTSSGGQREYVEFLSRAAVAAGVDGIFMEVHEE
CCCCEEECCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
PEQALCDGPNSVRLDDMPALLKKLKAIDAIVNQ
HHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TREITVGGVKIGGGRPLALVAGPCVIENETATLRCAERLMSICNGVGISLIFKASYDKA
CCEEEECCEEECCCCEEEEEECCEEEECCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
NRTSVTAFRGPGMKEGLRILAKVKEALGVPVLSDIHSIEQVEPAAEVLDVLQIPAFLCRQ
CCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCC
TDLLVAAGNTGKVINVKKGQFLAPWDMKNVVGKISSCDNDNIILTERGASFGYNNLVVDM
CCEEEEECCCCCEEEECCCCEECCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEC
RSFPIMRSTGYPVIFDATHSVQLPGGEGTSSGGQREYVEFLSRAAVAAGVDGIFMEVHEE
CCCCEEECCCCCEEEECCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCC
PEQALCDGPNSVRLDDMPALLKKLKAIDAIVNQ
HHHHHHCCCCCCEECCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA