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The map label for this gene is nifS [H]

Identifier: 197117956

GI number: 197117956

Start: 1817422

End: 1818591

Strand: Direct

Name: nifS [H]

Synonym: Gbem_1569

Alternate gene names: 197117956

Gene position: 1817422-1818591 (Clockwise)

Preceding gene: 197117955

Following gene: 197117957

Centisome position: 39.38

GC content: 64.1

Gene sequence:

>1170_bases
ATGAAAGAGATCTATCTTGACAACAACGCCACCACCAGGGTGGACGAGCGGGTTTTCGAGGAGATGCGTCCCTATTTCTG
CGAGCTGTACGGCAACCCGAGCTCAATGCACTTCTTCGGCGGTCAGGTGCAAAAGAAAGTGGACGAGGCACGCAGCCGCG
TCGCCGCGCTTCTGGGTGCGCTCCCCGACGAGATCGTCTTCACCGCCTGCGGGACCGAGAGCGACAACGCCGCCATTCGT
TCCGCGCTGGAGGTCTTCCCCGAGAAGCGCCACATCATAACCAGCCGTGTCGAGCACCCCGCGGTGCTCACCCAGTGCCG
CAACCTCACCAAGCGCGGTTACCGGGTCACCGAACTGAACGTGGACGGCAACGGGCAACTCGACCTCAAGGAACTCGAGG
CGGCGCTGGATGACGATACCGTCATCGTCTCCCTCATGTACGCCAACAACGAGACCGGCGTCATCTTCCCCATCGAGGAA
GCCGCCAAGATGGTGAAGGCAAAGGGCGCGCTCTTCCACACCGACGCGGTTCAGGCCGTGGGCAAGATCCCGCTCAACAT
GGCTGAATCCGCCATCGACCTGCTTTCCCTCTCCGGGCACAAGCTGCACGCCCCCAAAGGGGTAGGCGTTCTTTACGTGC
GCCGCGGCACGCCGTTCCGTCCGCTTCTGGTCGGCGGGCACCAGGAGCGCGGACGCAGGGCGGGGACCGAGAACACCGCC
TCCATCATCGCCATGGGCAAGGCCTGCGAGCTTGCCCACCTGCACATGCCCGAGGAAGCGGGGCGCGTGCGCGAGATGCG
CGACAGGCTGGAGCGCGAACTGACCGCGCTCATCCCCAACACCAGGATCAACGGCGGCGGCACCAACCGCCTCCCCAACA
CCCTTTCCATCGCCATGGAGTTCGTGGAAGGGGAGGGGATACTGCTGCTCCTCTCCGAGAAGGGAATCTGCGCCTCCTCC
GGCAGTGCCTGCACCTCGGGCTCCCTCGAGCCGTCCCACGTGCTGCGCGCTATGGGCGTTCCCTTTACCTGCGCCCACGG
CTCCATCCGCTTCTCGCTTTCCAGGTTTACCACCGACGCCGAGATCGATGCCGTGATCAAGGAACTCCCCCCGATCATCA
GCCGCCTGCGCCAGATGTCGCCGTTTGGCAGGGAGTTCCTGAACAAGTAG

Upstream 100 bases:

>100_bases
CATGCGCCGGCTGCGCCTCTTCCGGCAACACCGCGAAATTTGTCGAGCAGAAGCTGCGTGAGATGGTTTCTCCTGACATC
ACCGTGCAGGAGGTTCAGGG

Downstream 100 bases:

>100_bases
GACCCCGCTGTCAGATTCATAATGACCAAGGGGTGAGCGACTTTCGCTCACCCCTTTATTTATGCTCTATTAGCGAACGG
TGTTGGTACCACACTGGCCT

Product: nitrogen fixation iron-sulfur cluster assembly protein NifS

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein nifS1 [H]

Number of amino acids: Translated: 389; Mature: 389

Protein sequence:

>389_residues
MKEIYLDNNATTRVDERVFEEMRPYFCELYGNPSSMHFFGGQVQKKVDEARSRVAALLGALPDEIVFTACGTESDNAAIR
SALEVFPEKRHIITSRVEHPAVLTQCRNLTKRGYRVTELNVDGNGQLDLKELEAALDDDTVIVSLMYANNETGVIFPIEE
AAKMVKAKGALFHTDAVQAVGKIPLNMAESAIDLLSLSGHKLHAPKGVGVLYVRRGTPFRPLLVGGHQERGRRAGTENTA
SIIAMGKACELAHLHMPEEAGRVREMRDRLERELTALIPNTRINGGGTNRLPNTLSIAMEFVEGEGILLLLSEKGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFTTDAEIDAVIKELPPIISRLRQMSPFGREFLNK

Sequences:

>Translated_389_residues
MKEIYLDNNATTRVDERVFEEMRPYFCELYGNPSSMHFFGGQVQKKVDEARSRVAALLGALPDEIVFTACGTESDNAAIR
SALEVFPEKRHIITSRVEHPAVLTQCRNLTKRGYRVTELNVDGNGQLDLKELEAALDDDTVIVSLMYANNETGVIFPIEE
AAKMVKAKGALFHTDAVQAVGKIPLNMAESAIDLLSLSGHKLHAPKGVGVLYVRRGTPFRPLLVGGHQERGRRAGTENTA
SIIAMGKACELAHLHMPEEAGRVREMRDRLERELTALIPNTRINGGGTNRLPNTLSIAMEFVEGEGILLLLSEKGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFTTDAEIDAVIKELPPIISRLRQMSPFGREFLNK
>Mature_389_residues
MKEIYLDNNATTRVDERVFEEMRPYFCELYGNPSSMHFFGGQVQKKVDEARSRVAALLGALPDEIVFTACGTESDNAAIR
SALEVFPEKRHIITSRVEHPAVLTQCRNLTKRGYRVTELNVDGNGQLDLKELEAALDDDTVIVSLMYANNETGVIFPIEE
AAKMVKAKGALFHTDAVQAVGKIPLNMAESAIDLLSLSGHKLHAPKGVGVLYVRRGTPFRPLLVGGHQERGRRAGTENTA
SIIAMGKACELAHLHMPEEAGRVREMRDRLERELTALIPNTRINGGGTNRLPNTLSIAMEFVEGEGILLLLSEKGICASS
GSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFTTDAEIDAVIKELPPIISRLRQMSPFGREFLNK

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=44.300518134715, Blast_Score=336, Evalue=2e-92,
Organism=Homo sapiens, GI156713448, Length=417, Percent_Identity=34.5323741007194, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI48994898, Length=389, Percent_Identity=47.0437017994859, Blast_Score=338, Evalue=5e-94,
Organism=Escherichia coli, GI1789175, Length=388, Percent_Identity=27.319587628866, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787970, Length=392, Percent_Identity=25.2551020408163, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=384, Percent_Identity=42.1875, Blast_Score=298, Evalue=5e-81,
Organism=Caenorhabditis elegans, GI17533177, Length=316, Percent_Identity=34.4936708860759, Blast_Score=173, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=43.3766233766234, Blast_Score=310, Evalue=2e-85,
Organism=Drosophila melanogaster, GI20129463, Length=385, Percent_Identity=43.6363636363636, Blast_Score=318, Evalue=4e-87,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42626; Mature: 42626

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEIYLDNNATTRVDERVFEEMRPYFCELYGNPSSMHFFGGQVQKKVDEARSRVAALLGA
CCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHC
LPDEIVFTACGTESDNAAIRSALEVFPEKRHIITSRVEHPAVLTQCRNLTKRGYRVTELN
CCCHHEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEE
VDGNGQLDLKELEAALDDDTVIVSLMYANNETGVIFPIEEAAKMVKAKGALFHTDAVQAV
ECCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHH
GKIPLNMAESAIDLLSLSGHKLHAPKGVGVLYVRRGTPFRPLLVGGHQERGRRAGTENTA
HCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCC
SIIAMGKACELAHLHMPEEAGRVREMRDRLERELTALIPNTRINGGGTNRLPNTLSIAME
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
FVEGEGILLLLSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFTTDA
HCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEHHHHCCCH
EIDAVIKELPPIISRLRQMSPFGREFLNK
HHHHHHHHHHHHHHHHHHCCHHHHHHHCC
>Mature Secondary Structure
MKEIYLDNNATTRVDERVFEEMRPYFCELYGNPSSMHFFGGQVQKKVDEARSRVAALLGA
CCEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHC
LPDEIVFTACGTESDNAAIRSALEVFPEKRHIITSRVEHPAVLTQCRNLTKRGYRVTELN
CCCHHEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEEE
VDGNGQLDLKELEAALDDDTVIVSLMYANNETGVIFPIEEAAKMVKAKGALFHTDAVQAV
ECCCCCEEHHHHHHHCCCCEEEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEHHHHHHH
GKIPLNMAESAIDLLSLSGHKLHAPKGVGVLYVRRGTPFRPLLVGGHQERGRRAGTENTA
HCCCHHHHHHHHHHHHCCCCEEECCCCCCEEEEECCCCCCCEEECCCHHHHCCCCCCCCC
SIIAMGKACELAHLHMPEEAGRVREMRDRLERELTALIPNTRINGGGTNRLPNTLSIAME
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
FVEGEGILLLLSEKGICASSGSACTSGSLEPSHVLRAMGVPFTCAHGSIRFSLSRFTTDA
HCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEECCCCEEEEHHHHCCCH
EIDAVIKELPPIISRLRQMSPFGREFLNK
HHHHHHHHHHHHHHHHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7568132 [H]