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The map label for this gene is nifU [H]

Identifier: 197117955

GI number: 197117955

Start: 1816571

End: 1817425

Strand: Direct

Name: nifU [H]

Synonym: Gbem_1568

Alternate gene names: 197117955

Gene position: 1816571-1817425 (Clockwise)

Preceding gene: 308535249

Following gene: 197117956

Centisome position: 39.36

GC content: 60.47

Gene sequence:

>855_bases
ATGTGGGACTACACAGATAAAGTTAAAGAACATTTCCTCAACCCCCGGAACGTGGGAGAGATTACCGATGCGGATGCGGT
TGGCGAGGTGGGCAGCCTGGCCTGCGGCGACGCGCTTAAGCTGTTCATAAAGCTGGACGAGAAAAAAGAACGGATCGTCG
ATGCCAGGTTCCAGACCTTTGGTTGCGGCAGCGCCATCGCGTCTTCTTCCGCCCTCACCGAGATGGTGAAGGGGAAGACC
CTGGACGAGGCGCTGCAGATCAGCAACCAGGAGATCGCCGATTTCCTTGGGGGGCTCCCCGAGGAGAAGATGCACTGCTC
GGTCATGGGGCAGGAGGCGCTCGAGGTCGCCATCGCCAAATACCGCGGCGTCGAGGCGCCCAAGCACGGACACGACCACG
TCGAGACCGAGGGTGAGATCGTCTGCAAGTGCTTCGGCCTGACAGATATCTTCCTCAAGAAGGTCATCTCCTCCAACAAA
CTGCACACCGCCGAACAGGTGACCCACTTCACCAAGGCGGGCGGCGCCTGCGGCGGCTGCATCCCGAAGATCAAGGAGCT
GATCGCCGAGGTAATGGGCGAGGAGAAGAAGGCCGCGCCGGAGAGGCCGACGAAGCTCACCAACCTGAAGAAGATGCAGT
TGATCCAGGAGACCCTGGAGAACGAGATCCGTCCCCAGCTTTGGGCCGACGGCGGCGACCTCGAACTGATCGACATCGAC
GGTGCCAACGTGCAGGTCGCCTTCCGCAAGGCATGCGCCGGCTGCGCCTCTTCCGGCAACACCGCGAAATTTGTCGAGCA
GAAGCTGCGTGAGATGGTTTCTCCTGACATCACCGTGCAGGAGGTTCAGGGATGA

Upstream 100 bases:

>100_bases
GGTATACCTGACTGACGTGCTGTAGTTATAAGCGCGTTTTGGGGTTTTCTTTCTATCAGCTCTGCGCTATGATACCCGGA
CTAATTAGAAGGGAGGGTCT

Downstream 100 bases:

>100_bases
AAGAGATCTATCTTGACAACAACGCCACCACCAGGGTGGACGAGCGGGTTTTCGAGGAGATGCGTCCCTATTTCTGCGAG
CTGTACGGCAACCCGAGCTC

Product: nitrogen fixation iron-sulfur cluster assembly protein NifU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MWDYTDKVKEHFLNPRNVGEITDADAVGEVGSLACGDALKLFIKLDEKKERIVDARFQTFGCGSAIASSSALTEMVKGKT
LDEALQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGVEAPKHGHDHVETEGEIVCKCFGLTDIFLKKVISSNK
LHTAEQVTHFTKAGGACGGCIPKIKELIAEVMGEEKKAAPERPTKLTNLKKMQLIQETLENEIRPQLWADGGDLELIDID
GANVQVAFRKACAGCASSGNTAKFVEQKLREMVSPDITVQEVQG

Sequences:

>Translated_284_residues
MWDYTDKVKEHFLNPRNVGEITDADAVGEVGSLACGDALKLFIKLDEKKERIVDARFQTFGCGSAIASSSALTEMVKGKT
LDEALQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGVEAPKHGHDHVETEGEIVCKCFGLTDIFLKKVISSNK
LHTAEQVTHFTKAGGACGGCIPKIKELIAEVMGEEKKAAPERPTKLTNLKKMQLIQETLENEIRPQLWADGGDLELIDID
GANVQVAFRKACAGCASSGNTAKFVEQKLREMVSPDITVQEVQG
>Mature_284_residues
MWDYTDKVKEHFLNPRNVGEITDADAVGEVGSLACGDALKLFIKLDEKKERIVDARFQTFGCGSAIASSSALTEMVKGKT
LDEALQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAKYRGVEAPKHGHDHVETEGEIVCKCFGLTDIFLKKVISSNK
LHTAEQVTHFTKAGGACGGCIPKIKELIAEVMGEEKKAAPERPTKLTNLKKMQLIQETLENEIRPQLWADGGDLELIDID
GANVQVAFRKACAGCASSGNTAKFVEQKLREMVSPDITVQEVQG

Specific function: May be involved in the formation or repair of [Fe-S] clusters present in iron-sulfur proteins (Potential) [H]

COG id: COG0822

COG function: function code C; NifU homolog involved in Fe-S cluster formation

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifU family [H]

Homologues:

Organism=Homo sapiens, GI56699456, Length=130, Percent_Identity=53.0769230769231, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI24307953, Length=131, Percent_Identity=51.9083969465649, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1788878, Length=122, Percent_Identity=52.4590163934426, Blast_Score=120, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17543474, Length=122, Percent_Identity=49.1803278688525, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6324800, Length=131, Percent_Identity=47.3282442748092, Blast_Score=120, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6325122, Length=123, Percent_Identity=47.9674796747967, Blast_Score=115, Evalue=6e-27,
Organism=Drosophila melanogaster, GI21355597, Length=122, Percent_Identity=52.4590163934426, Blast_Score=121, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR016217
- InterPro:   IPR010238
- InterPro:   IPR001075
- InterPro:   IPR002871
- ProDom:   PD002830 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01106 NifU; PF01592 NifU_N [H]

EC number: NA

Molecular weight: Translated: 30920; Mature: 30920

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWDYTDKVKEHFLNPRNVGEITDADAVGEVGSLACGDALKLFIKLDEKKERIVDARFQTF
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GCGSAIASSSALTEMVKGKTLDEALQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAK
CCHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YRGVEAPKHGHDHVETEGEIVCKCFGLTDIFLKKVISSNKLHTAEQVTHFTKAGGACGGC
HCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCH
IPKIKELIAEVMGEEKKAAPERPTKLTNLKKMQLIQETLENEIRPQLWADGGDLELIDID
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEC
GANVQVAFRKACAGCASSGNTAKFVEQKLREMVSPDITVQEVQG
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHCCCC
>Mature Secondary Structure
MWDYTDKVKEHFLNPRNVGEITDADAVGEVGSLACGDALKLFIKLDEKKERIVDARFQTF
CCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
GCGSAIASSSALTEMVKGKTLDEALQISNQEIADFLGGLPEEKMHCSVMGQEALEVAIAK
CCHHHHHHHHHHHHHHCCCCHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YRGVEAPKHGHDHVETEGEIVCKCFGLTDIFLKKVISSNKLHTAEQVTHFTKAGGACGGC
HCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCH
IPKIKELIAEVMGEEKKAAPERPTKLTNLKKMQLIQETLENEIRPQLWADGGDLELIDID
HHHHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEC
GANVQVAFRKACAGCASSGNTAKFVEQKLREMVSPDITVQEVQG
CCCEEHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCEEHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7496536 [H]