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The map label for this gene is amtB [C]

Identifier: 197117895

GI number: 197117895

Start: 1746518

End: 1748065

Strand: Direct

Name: amtB [C]

Synonym: Gbem_1508

Alternate gene names: 197117895

Gene position: 1746518-1748065 (Clockwise)

Preceding gene: 197117894

Following gene: 197117896

Centisome position: 37.84

GC content: 64.21

Gene sequence:

>1548_bases
ATGATTCGTGACGATATGAAGGCTAAGAACGCAGCTCGCAACGCCGGTTTCTCTGTCGTTTCCAGCTGCCTCACCGTTAA
ATTCACCAAGCTCATGCTGCTGGCCTCCCTGCTGCTCCTCCCGGCAATGGCCGGCGCCGAGGAAAAAGCCGGCGAGGCGG
CCTCCCCCGCAGTTGCAGCGAGCGTTGCCGCGCCCGCCGCGCCGGTGGCAGCCACTGCCGCAGTACCCGCAGCACCAGCA
GCGGCTCCTGTCGCTGCAGCCGCCGTCCCTAAGAACGTCGACCCGGTGCTGAATACCGGCGACACCGCCTGGATGCTGAT
CTCCGCCGCACTGGTTCTCTTCATGATCCCCGGCCTCGCCCTTTTCTACGGCGGCATGGTCCGCTCCAAGAACGTCCTCT
CCACTATGATGCATTCGCTGGTGGCGATGGGGATCGTCGGGGTGCAGTGGGCCCTGATCGGCTACTCCCTTTCCTTCGGC
CCCGACCTGGGGCACGGTCTTATCGGCAACTTCTCGAAGGCGCTTTTGAGCGGCCTCATCAGCTTTAAGGACGGGAACCC
CGTCTACGCGCTGTTCCAGAACGTATCCACCGAGCCCGGCGCCATTCCCGAATACGTCTTCGCCATGTTCCAGGGGATGT
TCGCCATGATCACCGTGGCCCTCATCTCGGGTGCCCTGGCCGAGAGGATCAAGTTCTCCGCCTATTGTCTCTTCGTCCTC
CTTTGGACCACCTTGGTGTACGACCCGCTGGCCCACTGGGTCTGGATGGTGGATGGCTGGCTCTTCAAGCTCGGCGCGCT
CGACTTCGCAGGCGGCACCGTCGTTCACCTTTCCTCGGGTATCTCGTCCCTGGTGGTGCTCTACTTCTTGGGCAAGCGCC
ATGGCTTCCCGACCGAGCGCATGGCTCCGCACAGCCTGCCGCTGACCCTTTTGGGCGTCGGCGTGCTCTGGTTCGGCTGG
TTCGGCTTCAACGCCGGTTCCGCCGTGGTCGGCGCAAATTGCTCGGATGCCGCTGGCGGCCTGGCCGGCCTCGCCTTCAT
GACCACCACCATCGCTCCTGCGGCGGCAGGCCTTACCTGGATGATCGCCGAGTGGATCCATGCAGGGAAGCCTTCGGCCT
TGGGCTTTGGTTCCGGCGTCGTGGCGGGGCTCGTCGTGATCACCCCCGCTGCCGGCTTCGTGCAGCCGGGTGCCGCCCTC
CTGATGGGTGTTGCCGGCGGCCTCGTCTGCTATGGCGGCGTGCTCCTGAAAGCGAAGCTTAAGTACGACGACTCCCTCGA
TGCCTTCGGCGTGCACGGCATCGGCGGCACCACCGGCGCCATCCTGACCGGCGTCTTCGCCACCGTCGGAGCCACCGGTC
TTAGCTCGGGGAACATGAAGCAGTTCGTGACCCAGCTCGTCGCCGTCGGCGCCGCCGCCCTCTATGCCGTCGTGGTCACC
TTCGTCATCTCCTTCGTGCTGGACAAGACCATCGGCTTCAGGGTGGAGAAGGAAGACGAGATCATGGGGCTCGACCAGAC
GCAGCATTCGGAAAGTGCTTACAACTAG

Upstream 100 bases:

>100_bases
TTGCCGCTGGAAGACGCGGTCAGGATCAGGACCGGAGAGAAGGGCGGAGAGGCCATCTAAGCCCCTGCACCGACAACCAT
ACCGACCAATGGAGAGTGTT

Downstream 100 bases:

>100_bases
AATCTGATTTGCCAAAGTAAAGCCCGCGGGGGGACCAACAGGTCCCCCCGTTTTTTTTGCCCAAAAAAGTCTCCAACCTT
TAGAAATGCCGTTTTTTTCT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MIRDDMKAKNAARNAGFSVVSSCLTVKFTKLMLLASLLLLPAMAGAEEKAGEAASPAVAASVAAPAAPVAATAAVPAAPA
AAPVAAAAVPKNVDPVLNTGDTAWMLISAALVLFMIPGLALFYGGMVRSKNVLSTMMHSLVAMGIVGVQWALIGYSLSFG
PDLGHGLIGNFSKALLSGLISFKDGNPVYALFQNVSTEPGAIPEYVFAMFQGMFAMITVALISGALAERIKFSAYCLFVL
LWTTLVYDPLAHWVWMVDGWLFKLGALDFAGGTVVHLSSGISSLVVLYFLGKRHGFPTERMAPHSLPLTLLGVGVLWFGW
FGFNAGSAVVGANCSDAAGGLAGLAFMTTTIAPAAAGLTWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAL
LMGVAGGLVCYGGVLLKAKLKYDDSLDAFGVHGIGGTTGAILTGVFATVGATGLSSGNMKQFVTQLVAVGAAALYAVVVT
FVISFVLDKTIGFRVEKEDEIMGLDQTQHSESAYN

Sequences:

>Translated_515_residues
MIRDDMKAKNAARNAGFSVVSSCLTVKFTKLMLLASLLLLPAMAGAEEKAGEAASPAVAASVAAPAAPVAATAAVPAAPA
AAPVAAAAVPKNVDPVLNTGDTAWMLISAALVLFMIPGLALFYGGMVRSKNVLSTMMHSLVAMGIVGVQWALIGYSLSFG
PDLGHGLIGNFSKALLSGLISFKDGNPVYALFQNVSTEPGAIPEYVFAMFQGMFAMITVALISGALAERIKFSAYCLFVL
LWTTLVYDPLAHWVWMVDGWLFKLGALDFAGGTVVHLSSGISSLVVLYFLGKRHGFPTERMAPHSLPLTLLGVGVLWFGW
FGFNAGSAVVGANCSDAAGGLAGLAFMTTTIAPAAAGLTWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAL
LMGVAGGLVCYGGVLLKAKLKYDDSLDAFGVHGIGGTTGAILTGVFATVGATGLSSGNMKQFVTQLVAVGAAALYAVVVT
FVISFVLDKTIGFRVEKEDEIMGLDQTQHSESAYN
>Mature_515_residues
MIRDDMKAKNAARNAGFSVVSSCLTVKFTKLMLLASLLLLPAMAGAEEKAGEAASPAVAASVAAPAAPVAATAAVPAAPA
AAPVAAAAVPKNVDPVLNTGDTAWMLISAALVLFMIPGLALFYGGMVRSKNVLSTMMHSLVAMGIVGVQWALIGYSLSFG
PDLGHGLIGNFSKALLSGLISFKDGNPVYALFQNVSTEPGAIPEYVFAMFQGMFAMITVALISGALAERIKFSAYCLFVL
LWTTLVYDPLAHWVWMVDGWLFKLGALDFAGGTVVHLSSGISSLVVLYFLGKRHGFPTERMAPHSLPLTLLGVGVLWFGW
FGFNAGSAVVGANCSDAAGGLAGLAFMTTTIAPAAAGLTWMIAEWIHAGKPSALGFGSGVVAGLVVITPAAGFVQPGAAL
LMGVAGGLVCYGGVLLKAKLKYDDSLDAFGVHGIGGTTGAILTGVFATVGATGLSSGNMKQFVTQLVAVGAAALYAVVVT
FVISFVLDKTIGFRVEKEDEIMGLDQTQHSESAYN

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=461, Percent_Identity=43.3839479392625, Blast_Score=327, Evalue=9e-91,
Organism=Caenorhabditis elegans, GI17550182, Length=450, Percent_Identity=31.3333333333333, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17550180, Length=445, Percent_Identity=29.438202247191, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI133931100, Length=457, Percent_Identity=26.4770240700219, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI32564042, Length=442, Percent_Identity=27.8280542986425, Blast_Score=133, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6324187, Length=451, Percent_Identity=36.1419068736142, Blast_Score=257, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6325396, Length=431, Percent_Identity=35.4988399071926, Blast_Score=249, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321559, Length=429, Percent_Identity=33.1002331002331, Blast_Score=226, Evalue=8e-60,
Organism=Drosophila melanogaster, GI161078314, Length=433, Percent_Identity=32.7944572748268, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 52982; Mature: 52982

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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HHHHHHHHHHHCCEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]