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The map label for this gene is ubiE [H]

Identifier: 197117843

GI number: 197117843

Start: 1686704

End: 1687558

Strand: Direct

Name: ubiE [H]

Synonym: Gbem_1456

Alternate gene names: 197117843

Gene position: 1686704-1687558 (Clockwise)

Preceding gene: 197117842

Following gene: 197117844

Centisome position: 36.55

GC content: 65.61

Gene sequence:

>855_bases
ATGATGCCCGCGCACGAGCCGATGAGACCAGCCGCCGTCGCCGGCCACTACAGCTCCCCCGACCTGGAATCCAGGGTGCT
GGCGGCGCTGGTCGCGGCGGAAAAGAACCCGGACCAGCTGAGGCCGGAAGACCTCACCGCGATCGACGAATTCCACGTGC
GCGGGGCGGCGGCCACACAGGAGCTCGCCGCGGCGCTGGAGCCCGCGCCGGGACTGCTGGTCCTCGACGTCGGCTGTGGG
CTGGGAGGGGCTTCGCGCTATCTCTCGCGTCTATTGGACTGCCACGTCATCGGAGTCGACCAAAGCTCCGACTACTGCCA
CGTGGCGAGGATGCTCTCCGAGCGGCTCGGGATGAGCGACCGGGTCGCCTACCTCCAGGCCGACGCGCTGGAGCTCCCCT
TTGGCGACGCCGCCTTCGATCTGGTCTGGATCCAGCACCTGTCCATGAACATCGAGGACAAGCCGAAGCTCTACCGCGAG
ATCCGGCGGGTGCTGAAGCCGGGCGGCAGGCTCGCCTTCTACGAGATACTTGCAGGGCCCGGAGGAGAAGTACATTTTCC
GGTGCCGTGGGCAAGGGATCCGTCGGCGAGTTTTCTGGCAAGCCAGGAGGATTTGCGCCGGCTCTTGACGGAAGAAGGGT
TCCAGCCTCTGTTCTGGCAGGACGTGACCAAAGACGGGTTGGAGTGGTTTCGCCGCAGGCAGCAGAAGAGTCGTGAGGGT
GGGGCGAGCCCCTTCGGGTTGCACCTGCTACTTGGCGAGGGATTTCCGGCCATGGCCGGGAACCAGCTGCGCAACCTCGA
AGAGGGTAGGATCACCTTGGTCGAGGCGGTTTTCCAGGTGCCGCCCGATAACTGA

Upstream 100 bases:

>100_bases
ACCTGCGGCAAGTCGGCCACCGTAGCCGACGCGCCGGAAAAGAAGGCGCCGCGCCCGGCGCAGTTCACGGGCGCCTACGC
CGGAGCCTGAGGAGGCGTCC

Downstream 100 bases:

>100_bases
GATAGTGCCGGGGGACTGGCTCCGCAGGTGCCTGTCCCCCTTAGCGGTACCCCTCGGATTCACCAGCAGGTCAGGAGGTT
CACCATGAAAAGTAGCGCGA

Product: type 11 SAM-dependent methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MMPAHEPMRPAAVAGHYSSPDLESRVLAALVAAEKNPDQLRPEDLTAIDEFHVRGAAATQELAAALEPAPGLLVLDVGCG
LGGASRYLSRLLDCHVIGVDQSSDYCHVARMLSERLGMSDRVAYLQADALELPFGDAAFDLVWIQHLSMNIEDKPKLYRE
IRRVLKPGGRLAFYEILAGPGGEVHFPVPWARDPSASFLASQEDLRRLLTEEGFQPLFWQDVTKDGLEWFRRRQQKSREG
GASPFGLHLLLGEGFPAMAGNQLRNLEEGRITLVEAVFQVPPDN

Sequences:

>Translated_284_residues
MMPAHEPMRPAAVAGHYSSPDLESRVLAALVAAEKNPDQLRPEDLTAIDEFHVRGAAATQELAAALEPAPGLLVLDVGCG
LGGASRYLSRLLDCHVIGVDQSSDYCHVARMLSERLGMSDRVAYLQADALELPFGDAAFDLVWIQHLSMNIEDKPKLYRE
IRRVLKPGGRLAFYEILAGPGGEVHFPVPWARDPSASFLASQEDLRRLLTEEGFQPLFWQDVTKDGLEWFRRRQQKSREG
GASPFGLHLLLGEGFPAMAGNQLRNLEEGRITLVEAVFQVPPDN
>Mature_284_residues
MMPAHEPMRPAAVAGHYSSPDLESRVLAALVAAEKNPDQLRPEDLTAIDEFHVRGAAATQELAAALEPAPGLLVLDVGCG
LGGASRYLSRLLDCHVIGVDQSSDYCHVARMLSERLGMSDRVAYLQADALELPFGDAAFDLVWIQHLSMNIEDKPKLYRE
IRRVLKPGGRLAFYEILAGPGGEVHFPVPWARDPSASFLASQEDLRRLLTEEGFQPLFWQDVTKDGLEWFRRRQQKSREG
GASPFGLHLLLGEGFPAMAGNQLRNLEEGRITLVEAVFQVPPDN

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=108, Percent_Identity=37.037037037037, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17561380, Length=157, Percent_Identity=31.2101910828025, Blast_Score=71, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25143945, Length=116, Percent_Identity=30.1724137931034, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6323635, Length=105, Percent_Identity=35.2380952380952, Blast_Score=76, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31333; Mature: 31333

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMPAHEPMRPAAVAGHYSSPDLESRVLAALVAAEKNPDQLRPEDLTAIDEFHVRGAAATQ
CCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHH
ELAAALEPAPGLLVLDVGCGLGGASRYLSRLLDCHVIGVDQSSDYCHVARMLSERLGMSD
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCH
RVAYLQADALELPFGDAAFDLVWIQHLSMNIEDKPKLYREIRRVLKPGGRLAFYEILAGP
HHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GGEVHFPVPWARDPSASFLASQEDLRRLLTEEGFQPLFWQDVTKDGLEWFRRRQQKSREG
CCCEEECCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GASPFGLHLLLGEGFPAMAGNQLRNLEEGRITLVEAVFQVPPDN
CCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MMPAHEPMRPAAVAGHYSSPDLESRVLAALVAAEKNPDQLRPEDLTAIDEFHVRGAAATQ
CCCCCCCCCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCHHHHH
ELAAALEPAPGLLVLDVGCGLGGASRYLSRLLDCHVIGVDQSSDYCHVARMLSERLGMSD
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCH
RVAYLQADALELPFGDAAFDLVWIQHLSMNIEDKPKLYREIRRVLKPGGRLAFYEILAGP
HHHHHHHCCEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCC
GGEVHFPVPWARDPSASFLASQEDLRRLLTEEGFQPLFWQDVTKDGLEWFRRRQQKSREG
CCCEEECCCCCCCCCHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GASPFGLHLLLGEGFPAMAGNQLRNLEEGRITLVEAVFQVPPDN
CCCCCEEEEEECCCCCCCCCHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA