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The map label for this gene is maeB-2 [H]

Identifier: 197117842

GI number: 197117842

Start: 1684354

End: 1686693

Strand: Direct

Name: maeB-2 [H]

Synonym: Gbem_1455

Alternate gene names: 197117842

Gene position: 1684354-1686693 (Clockwise)

Preceding gene: 197117841

Following gene: 197117843

Centisome position: 36.5

GC content: 63.63

Gene sequence:

>2340_bases
ATGAAGAAGATGTCCGATGCACTGGAATACCATGCCACTGGCCGCAAGGGGAAGATCGAGGTAATCGCCACCAAGCCGTG
CCAGACCGCGCAGGACCTGTCGCTGGCCTATTCGCCGGGAGTCGCCGAGCCCTGCCTCGCCATCGAGCAGAACCCTGAGG
ACGCCTACCAGTACACCGCCAAGGGGAACTTGGTGGCCGTGGTATCCAACGGCACCGCCGTCCTTGGCCTCGGCAACATC
GGCGCGCTCGCGGGCAAGCCGGTCATGGAAGGGAAGGGGGTTCTCTTCAAGCGCTTCGCCGACGTCGACGTCTTCGATAT
CGAGTTGAACAGCGAGGACCCGGACGAGATCATCAAGGCCTGCCAGCTTCTGGAGCCCACCTTCGGCGGCATCAACCTGG
AGGACATCAAGGCGCCCGAGTGCTTCTACATCGAAGAGGAACTGAAGAAGACCATGAACATCCCGGTCTTCCACGACGAC
CAGCACGGAACGGCGATCATCTCGGCAGCCGGGCTCATCAACGCGCTCGAACTGGTCGGCAAGAAGATAGAGGACGTGAA
GATAGTGGTGAACGGCGCAGGCGCATCGGCCAACGCATGCGCGAACCTCGCCATCTCCCTGGGCCTCAAGCTGGAAAACC
TCATCATGTGCGACACCAAGGGGGTCATCTACAAGGGGAGAGTGGAGGGGATGAACAAGTACAAGGAGCGCTTCGCCGCC
GACACCCCGCTGCGCACCCTGGAAGAGGCGGCGGTCGGCTGCGACGTCATGTATGGTCTCTCCTCCAAAGGGGCCATCAC
CCAGGACATGGTGCGCAGCATGGCGGCCAACCCCATCATCTTCGCCATGGCGAATCCGGACCCCGAGATCACCCCGGAAG
AGGCGCACGCGGTCCGCGGCGACGTACTCATCGCCACCGGGCGCTCCGACTATCCGAACCAGGTCAACAACGTGCTGGGC
TTCCCGTTCATCTTCCGCGGCGCGCTCGACGTGCGGGCCACCACCATCAACGAAGAGATGAAAAAGGCGGCAGTCTTCGC
CCTGGCGGAACTCGCCCGCGAGGAATGCCCCGACTCCGTCTGCCGCGCCTATGGCGACGTCAAGTTCAGCTTCGGCCGCG
ATTACATCATCCCCAAACCCTTCGACCCCCGGGCGCTGTTGCGGGTCGCCCCGGCCATAGCCAAGGCGGCAATGGAGACG
GGGGTGGCGCGCCAGCCCATCGCGGACATGAACAAGTACGTGGAGCATCTGGAGTCGCTGCAGGGCAAATCCAAGGAGAC
GCTGCGCCAGATCATCAACAAGGCCAAATGCGATCCGAAGACCGTGGTCTTCCCCGAGGGCGAAAACGACAAGGTGCTGC
GCGCAGCGCAGATGCTGGTCGAGCAGGGGATCGCGCGGCCGATCCTTCTGGGGAACGAAGACAAGATCCGCTGCACCCTG
AAACATCTCGACATCGACCTGAACGGCGGGGTCACCATCATCGACCCGGCCACCTCGGAACACTCCGAATCGTACGCCAG
CGAACTCTTCCTGCTCAGGCAGAGAAAGGGGCTCACCCTCTCCGAGGCCCGCAGGCTCATCAGCCGCAAGTCGCGCACCC
ACTTCGGCTGCATGATGGTGCGCCGCGGCCACGCCGACGCCCTTTTGGCCGGGGTCGACGCCAACTACGCCGACACCATC
CGCCCCGCGCTGCAGGTGATCGGCAAACAGGAAGGGCTCTCCAGCGTGCACGGCCTCTACATGATGGTCTTCAAGAAGGA
CATCTTCTTCCTGGCCGACACCACCGTCTGCATCGATCCGGATGCTGCGGAGCTGGCCGAGACGGCGATCCTCGCGGCGG
ACAAGGCGGCCATGCTCGGCATCGACCCGAGCGTCGCCATGCTCTCCATGTCCAACTTCGGCTCGGTGCGGCATCCGCAG
GCCGACCGGGTGAGAAGCGCCGTGGAGATGGTGAAAAAGAGGGCGCCCGGGCTCGACATCGACGGGGAGATGCAGGCGGA
TACCGCCGTCGTTTCCGAGATGCTGCAGGCGAATTTCCCCTTCACGACACTGAAAAAAGCTGCTAATGTCCTGGTCTTCC
CGGACCTTACTTCGGGGAACATCTGCTACAAGCTCCTGCGGCAGTTGGGCGGCGCCGACGCCATCGGTCCCATCCTCATG
GGGATGAACAAGCCGGTGCACATCCTGCAGCAGGGGGACGACGTCATGGACATAGTCAACATGGCGGCCATCGCCGTGGT
GGACGCGCAGACCTGCGGCAAGTCGGCCACCGTAGCCGACGCGCCGGAAAAGAAGGCGCCGCGCCCGGCGCAGTTCACGG
GCGCCTACGCCGGAGCCTGA

Upstream 100 bases:

>100_bases
CCGGGGGGAGGAGTAGCGGAGATCGTCGTCAATTGTCAATTATCAATTGTCAATTGCCGTTGGTTATCAATTGTCAATTG
CCTTTGGAGGGTTCGCCGAT

Downstream 100 bases:

>100_bases
GGAGGCGTCCATGATGCCCGCGCACGAGCCGATGAGACCAGCCGCCGTCGCCGGCCACTACAGCTCCCCCGACCTGGAAT
CCAGGGTGCTGGCGGCGCTG

Product: malic enzyme

Products: NA

Alternate protein names: NADP-ME [H]

Number of amino acids: Translated: 779; Mature: 779

Protein sequence:

>779_residues
MKKMSDALEYHATGRKGKIEVIATKPCQTAQDLSLAYSPGVAEPCLAIEQNPEDAYQYTAKGNLVAVVSNGTAVLGLGNI
GALAGKPVMEGKGVLFKRFADVDVFDIELNSEDPDEIIKACQLLEPTFGGINLEDIKAPECFYIEEELKKTMNIPVFHDD
QHGTAIISAAGLINALELVGKKIEDVKIVVNGAGASANACANLAISLGLKLENLIMCDTKGVIYKGRVEGMNKYKERFAA
DTPLRTLEEAAVGCDVMYGLSSKGAITQDMVRSMAANPIIFAMANPDPEITPEEAHAVRGDVLIATGRSDYPNQVNNVLG
FPFIFRGALDVRATTINEEMKKAAVFALAELAREECPDSVCRAYGDVKFSFGRDYIIPKPFDPRALLRVAPAIAKAAMET
GVARQPIADMNKYVEHLESLQGKSKETLRQIINKAKCDPKTVVFPEGENDKVLRAAQMLVEQGIARPILLGNEDKIRCTL
KHLDIDLNGGVTIIDPATSEHSESYASELFLLRQRKGLTLSEARRLISRKSRTHFGCMMVRRGHADALLAGVDANYADTI
RPALQVIGKQEGLSSVHGLYMMVFKKDIFFLADTTVCIDPDAAELAETAILAADKAAMLGIDPSVAMLSMSNFGSVRHPQ
ADRVRSAVEMVKKRAPGLDIDGEMQADTAVVSEMLQANFPFTTLKKAANVLVFPDLTSGNICYKLLRQLGGADAIGPILM
GMNKPVHILQQGDDVMDIVNMAAIAVVDAQTCGKSATVADAPEKKAPRPAQFTGAYAGA

Sequences:

>Translated_779_residues
MKKMSDALEYHATGRKGKIEVIATKPCQTAQDLSLAYSPGVAEPCLAIEQNPEDAYQYTAKGNLVAVVSNGTAVLGLGNI
GALAGKPVMEGKGVLFKRFADVDVFDIELNSEDPDEIIKACQLLEPTFGGINLEDIKAPECFYIEEELKKTMNIPVFHDD
QHGTAIISAAGLINALELVGKKIEDVKIVVNGAGASANACANLAISLGLKLENLIMCDTKGVIYKGRVEGMNKYKERFAA
DTPLRTLEEAAVGCDVMYGLSSKGAITQDMVRSMAANPIIFAMANPDPEITPEEAHAVRGDVLIATGRSDYPNQVNNVLG
FPFIFRGALDVRATTINEEMKKAAVFALAELAREECPDSVCRAYGDVKFSFGRDYIIPKPFDPRALLRVAPAIAKAAMET
GVARQPIADMNKYVEHLESLQGKSKETLRQIINKAKCDPKTVVFPEGENDKVLRAAQMLVEQGIARPILLGNEDKIRCTL
KHLDIDLNGGVTIIDPATSEHSESYASELFLLRQRKGLTLSEARRLISRKSRTHFGCMMVRRGHADALLAGVDANYADTI
RPALQVIGKQEGLSSVHGLYMMVFKKDIFFLADTTVCIDPDAAELAETAILAADKAAMLGIDPSVAMLSMSNFGSVRHPQ
ADRVRSAVEMVKKRAPGLDIDGEMQADTAVVSEMLQANFPFTTLKKAANVLVFPDLTSGNICYKLLRQLGGADAIGPILM
GMNKPVHILQQGDDVMDIVNMAAIAVVDAQTCGKSATVADAPEKKAPRPAQFTGAYAGA
>Mature_779_residues
MKKMSDALEYHATGRKGKIEVIATKPCQTAQDLSLAYSPGVAEPCLAIEQNPEDAYQYTAKGNLVAVVSNGTAVLGLGNI
GALAGKPVMEGKGVLFKRFADVDVFDIELNSEDPDEIIKACQLLEPTFGGINLEDIKAPECFYIEEELKKTMNIPVFHDD
QHGTAIISAAGLINALELVGKKIEDVKIVVNGAGASANACANLAISLGLKLENLIMCDTKGVIYKGRVEGMNKYKERFAA
DTPLRTLEEAAVGCDVMYGLSSKGAITQDMVRSMAANPIIFAMANPDPEITPEEAHAVRGDVLIATGRSDYPNQVNNVLG
FPFIFRGALDVRATTINEEMKKAAVFALAELAREECPDSVCRAYGDVKFSFGRDYIIPKPFDPRALLRVAPAIAKAAMET
GVARQPIADMNKYVEHLESLQGKSKETLRQIINKAKCDPKTVVFPEGENDKVLRAAQMLVEQGIARPILLGNEDKIRCTL
KHLDIDLNGGVTIIDPATSEHSESYASELFLLRQRKGLTLSEARRLISRKSRTHFGCMMVRRGHADALLAGVDANYADTI
RPALQVIGKQEGLSSVHGLYMMVFKKDIFFLADTTVCIDPDAAELAETAILAADKAAMLGIDPSVAMLSMSNFGSVRHPQ
ADRVRSAVEMVKKRAPGLDIDGEMQADTAVVSEMLQANFPFTTLKKAANVLVFPDLTSGNICYKLLRQLGGADAIGPILM
GMNKPVHILQQGDDVMDIVNMAAIAVVDAQTCGKSATVADAPEKKAPRPAQFTGAYAGA

Specific function: Unknown

COG id: COG0281

COG function: function code C; Malic enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the phosphate acetyltransferase and butyryltransferase family [H]

Homologues:

Organism=Homo sapiens, GI62420882, Length=324, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=4e-17,
Organism=Homo sapiens, GI62420880, Length=324, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=4e-17,
Organism=Homo sapiens, GI239049447, Length=324, Percent_Identity=29.6296296296296, Blast_Score=88, Evalue=4e-17,
Organism=Homo sapiens, GI4505143, Length=345, Percent_Identity=28.4057971014493, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI4505145, Length=319, Percent_Identity=29.4670846394984, Blast_Score=70, Evalue=6e-12,
Organism=Homo sapiens, GI270265879, Length=320, Percent_Identity=29.375, Blast_Score=70, Evalue=9e-12,
Organism=Escherichia coli, GI1788806, Length=750, Percent_Identity=52.2666666666667, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1788635, Length=343, Percent_Identity=34.402332361516, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1788800, Length=318, Percent_Identity=36.7924528301887, Blast_Score=190, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17537199, Length=424, Percent_Identity=26.1792452830189, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322823, Length=268, Percent_Identity=28.7313432835821, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24646388, Length=353, Percent_Identity=30.028328611898, Blast_Score=98, Evalue=2e-20,
Organism=Drosophila melanogaster, GI24646386, Length=350, Percent_Identity=29.7142857142857, Blast_Score=98, Evalue=3e-20,
Organism=Drosophila melanogaster, GI78707242, Length=407, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI78707238, Length=407, Percent_Identity=27.027027027027, Blast_Score=86, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356279, Length=396, Percent_Identity=26.2626262626263, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281362672, Length=396, Percent_Identity=26.2626262626263, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281362674, Length=396, Percent_Identity=26.2626262626263, Blast_Score=83, Evalue=7e-16,
Organism=Drosophila melanogaster, GI78707240, Length=345, Percent_Identity=27.8260869565217, Blast_Score=81, Evalue=3e-15,
Organism=Drosophila melanogaster, GI78707232, Length=376, Percent_Identity=25, Blast_Score=69, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363503, Length=376, Percent_Identity=25, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI281363505, Length=376, Percent_Identity=25, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI78707236, Length=376, Percent_Identity=25, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015884
- InterPro:   IPR012301
- InterPro:   IPR012302
- InterPro:   IPR012188
- InterPro:   IPR016040
- InterPro:   IPR002505 [H]

Pfam domain/function: PF00390 malic; PF03949 Malic_M; PF01515 PTA_PTB [H]

EC number: =1.1.1.40 [H]

Molecular weight: Translated: 83976; Mature: 83976

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS00331 MALIC_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKMSDALEYHATGRKGKIEVIATKPCQTAQDLSLAYSPGVAEPCLAIEQNPEDAYQYTA
CCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCEEECCCCCCCCCEECCCCHHHHEEEC
KGNLVAVVSNGTAVLGLGNIGALAGKPVMEGKGVLFKRFADVDVFDIELNSEDPDEIIKA
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHH
CQLLEPTFGGINLEDIKAPECFYIEEELKKTMNIPVFHDDQHGTAIISAAGLINALELVG
HHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHC
KKIEDVKIVVNGAGASANACANLAISLGLKLENLIMCDTKGVIYKGRVEGMNKYKERFAA
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEECEEEEECCCCCEEECHHHHHHHHHHHHCC
DTPLRTLEEAAVGCDVMYGLSSKGAITQDMVRSMAANPIIFAMANPDPEITPEEAHAVRG
CCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCC
DVLIATGRSDYPNQVNNVLGFPFIFRGALDVRATTINEEMKKAAVFALAELAREECPDSV
CEEEEECCCCCCHHHHHHCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
CRAYGDVKFSFGRDYIIPKPFDPRALLRVAPAIAKAAMETGVARQPIADMNKYVEHLESL
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QGKSKETLRQIINKAKCDPKTVVFPEGENDKVLRAAQMLVEQGIARPILLGNEDKIRCTL
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEE
KHLDIDLNGGVTIIDPATSEHSESYASELFLLRQRKGLTLSEARRLISRKSRTHFGCMMV
EEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHEEEEE
RRGHADALLAGVDANYADTIRPALQVIGKQEGLSSVHGLYMMVFKKDIFFLADTTVCIDP
ECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEECCEEEECC
DAAELAETAILAADKAAMLGIDPSVAMLSMSNFGSVRHPQADRVRSAVEMVKKRAPGLDI
CHHHHHHHHHHHCCCHHEECCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DGEMQADTAVVSEMLQANFPFTTLKKAANVLVFPDLTSGNICYKLLRQLGGADAIGPILM
CCCCCHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
GMNKPVHILQQGDDVMDIVNMAAIAVVDAQTCGKSATVADAPEKKAPRPAQFTGAYAGA
CCCCCEEEECCCCHHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKKMSDALEYHATGRKGKIEVIATKPCQTAQDLSLAYSPGVAEPCLAIEQNPEDAYQYTA
CCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHCCEEECCCCCCCCCEECCCCHHHHEEEC
KGNLVAVVSNGTAVLGLGNIGALAGKPVMEGKGVLFKRFADVDVFDIELNSEDPDEIIKA
CCCEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEEECCCEEEEEEECCCCHHHHHHH
CQLLEPTFGGINLEDIKAPECFYIEEELKKTMNIPVFHDDQHGTAIISAAGLINALELVG
HHHHCCCCCCCCHHHCCCCCEEEEHHHHHHHCCCCEEECCCCCCEEEHHHHHHHHHHHHC
KKIEDVKIVVNGAGASANACANLAISLGLKLENLIMCDTKGVIYKGRVEGMNKYKERFAA
CCCCEEEEEEECCCCCCHHHHHHHHHCCCEECEEEEECCCCCEEECHHHHHHHHHHHHCC
DTPLRTLEEAAVGCDVMYGLSSKGAITQDMVRSMAANPIIFAMANPDPEITPEEAHAVRG
CCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHCCC
DVLIATGRSDYPNQVNNVLGFPFIFRGALDVRATTINEEMKKAAVFALAELAREECPDSV
CEEEEECCCCCCHHHHHHCCCHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHH
CRAYGDVKFSFGRDYIIPKPFDPRALLRVAPAIAKAAMETGVARQPIADMNKYVEHLESL
HHHHCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
QGKSKETLRQIINKAKCDPKTVVFPEGENDKVLRAAQMLVEQGIARPILLGNEDKIRCTL
CCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCEEECCCCCEEEEE
KHLDIDLNGGVTIIDPATSEHSESYASELFLLRQRKGLTLSEARRLISRKSRTHFGCMMV
EEEEEEECCCEEEECCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCHHEEEEE
RRGHADALLAGVDANYADTIRPALQVIGKQEGLSSVHGLYMMVFKKDIFFLADTTVCIDP
ECCCCCEEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCEEEEECCEEEECC
DAAELAETAILAADKAAMLGIDPSVAMLSMSNFGSVRHPQADRVRSAVEMVKKRAPGLDI
CHHHHHHHHHHHCCCHHEECCCCCHHEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DGEMQADTAVVSEMLQANFPFTTLKKAANVLVFPDLTSGNICYKLLRQLGGADAIGPILM
CCCCCHHHHHHHHHHHCCCCHHHHHHHCCEEEECCCCCCHHHHHHHHHHCCCCCHHHHHH
GMNKPVHILQQGDDVMDIVNMAAIAVVDAQTCGKSATVADAPEKKAPRPAQFTGAYAGA
CCCCCEEEECCCCHHHHHHHHHHHHEEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 36376 [H]