Click here to switch to the map view.

The map label for this gene is fadA [H]

Identifier: 197117803

GI number: 197117803

Start: 1631727

End: 1632917

Strand: Direct

Name: fadA [H]

Synonym: Gbem_1415

Alternate gene names: 197117803

Gene position: 1631727-1632917 (Clockwise)

Preceding gene: 308535238

Following gene: 197117804

Centisome position: 35.36

GC content: 65.74

Gene sequence:

>1191_bases
ATGCTTAAGAAAGCGTACATACCCTACCGCGGTTACTACAGCACCCCCTTCGCCAAGTGGCAGGGGACCCTCGCCAACGA
ACACTCCATAGTCTTTGGCGCCGCCACTTCCAAGAGGTTTTTTGAGAGCAAGGGGTGGGACCCCAAGAGCATCGAGTACC
TGCTGGTGGGGAGCACCGTGTACCAGAAGCAGTGGTTCTACAGCGGGCCGTGGGCCGCGGCCCTGATGGGGGCCGAAGCC
ACCCCCGGCATCCTGGTGAGCCAGGCCTGCTCCACCTCCACCTTTTCCATCTACCAGGCGGGTATGGGGGTTGAGACCGG
TATGTTCGAGAACAGCTGGTGCCTCATGGCCGACCGCCTTTCCAACGGCCCGCACGCCGCCTGGCCCAACCCCGCGGGCC
CCGGCGGCCAGCAGATCGCCGAGGACTGGGTCATGGACAACTTCGGCAGGGACCCCTGGGCCGGCGAGGCCATGATCCAG
ACCGCGGAAAACGTGGCCAAGGAGTACGGGGTGACCCGCGAGGAGTGCGACGCGCTCACGCTGCGACGCCAGGAGCAGTA
CCTCGATTCCCTCGCCAACGACCGCGAGTTCCAGAAGAGGTACCTGTTCCCGGCCCAGGTGCAGCTCTCAAAGAAGAAAT
CGACCCTCCTGGAGGCCGACGAGGGGGTTGTCACCAGCACCAGGGAAGGGCTTGCCTCCCTGAACCCGGTCCTCCCCGGC
GGGGTGCACACCTTCGGCGCCCAGACCCACCCGGCCGACGGCAACGCGGGGATCTGCGTCACCACACGCGAGAAGGCGAG
GGAGCTCTCCGCAGACCCGGGCGTCGTGGTGCAGTTGGTCTCCTTCGGATTCGCCCGGACCAAGAAGGCGCACATGGCTG
CGGCCGTGGCCCCGTCGGCGCAGATGGCGCTCAATGGGGCAGGGATCAAGGCGTCCGACCTGGGCGCGGTGAAGACCCAC
AACCCCTTCGCCGCGAACGACATCGTCATGGCCAAGGTGATGGGGCTCGATCTCGACAGCATGAACAACTACGGCTCCTC
GCTCATCTTCGGCCACCCCCAGGCCGCCACCGGCGCGAGGCTCATCGTCGAGGGGATCGAGGAACTGGCCCAGAAGGGGG
GAGGCTACCTCCTCTTCTCCGGCTGCGCGGCAGGCGACACCGCAGCCTCCCTCGTTTTGAAGGTTAATTAG

Upstream 100 bases:

>100_bases
CCCGGTCATGGCGTTCGAAAAAGGGGCCTTTGCCGTCGACGGCAGGATCAGGATCTAATCCGCACCGACGGCCAACAATA
AACCAACAGGAGGAACTTCG

Downstream 100 bases:

>100_bases
AAAAGGGGGGCAGGAGCTCTCCCCCTCCCTTGACGGGAGGGGGCAGGGGGGTGGGTGAACCTGGCAGTAGCGCAAAAGGT
GGCACCTACCCCCACCCCCT

Product: thiolase

Products: NA

Alternate protein names: Acetyl-CoA acyltransferase; Beta-ketothiolase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLKKAYIPYRGYYSTPFAKWQGTLANEHSIVFGAATSKRFFESKGWDPKSIEYLLVGSTVYQKQWFYSGPWAAALMGAEA
TPGILVSQACSTSTFSIYQAGMGVETGMFENSWCLMADRLSNGPHAAWPNPAGPGGQQIAEDWVMDNFGRDPWAGEAMIQ
TAENVAKEYGVTREECDALTLRRQEQYLDSLANDREFQKRYLFPAQVQLSKKKSTLLEADEGVVTSTREGLASLNPVLPG
GVHTFGAQTHPADGNAGICVTTREKARELSADPGVVVQLVSFGFARTKKAHMAAAVAPSAQMALNGAGIKASDLGAVKTH
NPFAANDIVMAKVMGLDLDSMNNYGSSLIFGHPQAATGARLIVEGIEELAQKGGGYLLFSGCAAGDTAASLVLKVN

Sequences:

>Translated_396_residues
MLKKAYIPYRGYYSTPFAKWQGTLANEHSIVFGAATSKRFFESKGWDPKSIEYLLVGSTVYQKQWFYSGPWAAALMGAEA
TPGILVSQACSTSTFSIYQAGMGVETGMFENSWCLMADRLSNGPHAAWPNPAGPGGQQIAEDWVMDNFGRDPWAGEAMIQ
TAENVAKEYGVTREECDALTLRRQEQYLDSLANDREFQKRYLFPAQVQLSKKKSTLLEADEGVVTSTREGLASLNPVLPG
GVHTFGAQTHPADGNAGICVTTREKARELSADPGVVVQLVSFGFARTKKAHMAAAVAPSAQMALNGAGIKASDLGAVKTH
NPFAANDIVMAKVMGLDLDSMNNYGSSLIFGHPQAATGARLIVEGIEELAQKGGGYLLFSGCAAGDTAASLVLKVN
>Mature_396_residues
MLKKAYIPYRGYYSTPFAKWQGTLANEHSIVFGAATSKRFFESKGWDPKSIEYLLVGSTVYQKQWFYSGPWAAALMGAEA
TPGILVSQACSTSTFSIYQAGMGVETGMFENSWCLMADRLSNGPHAAWPNPAGPGGQQIAEDWVMDNFGRDPWAGEAMIQ
TAENVAKEYGVTREECDALTLRRQEQYLDSLANDREFQKRYLFPAQVQLSKKKSTLLEADEGVVTSTREGLASLNPVLPG
GVHTFGAQTHPADGNAGICVTTREKARELSADPGVVVQLVSFGFARTKKAHMAAAVAPSAQMALNGAGIKASDLGAVKTH
NPFAANDIVMAKVMGLDLDSMNNYGSSLIFGHPQAATGARLIVEGIEELAQKGGGYLLFSGCAAGDTAASLVLKVN

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=387, Percent_Identity=22.7390180878553, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI4501853, Length=323, Percent_Identity=25.3869969040248, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI4504327, Length=258, Percent_Identity=25.5813953488372, Blast_Score=76, Evalue=6e-14,
Organism=Homo sapiens, GI4557237, Length=387, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788554, Length=389, Percent_Identity=25.706940874036, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87082165, Length=389, Percent_Identity=23.3933161953728, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1788683, Length=263, Percent_Identity=30.7984790874525, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1787663, Length=243, Percent_Identity=25.9259259259259, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI48994986, Length=228, Percent_Identity=27.1929824561404, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=23.1362467866324, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI25147385, Length=394, Percent_Identity=23.3502538071066, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17535921, Length=360, Percent_Identity=23.0555555555556, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17551802, Length=252, Percent_Identity=26.5873015873016, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6325229, Length=396, Percent_Identity=23.2323232323232, Blast_Score=76, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6322031, Length=322, Percent_Identity=22.9813664596273, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI17648125, Length=250, Percent_Identity=28.8, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137578, Length=397, Percent_Identity=25.4408060453401, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24640423, Length=392, Percent_Identity=23.469387755102, Blast_Score=76, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 42427; Mature: 42427

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKAYIPYRGYYSTPFAKWQGTLANEHSIVFGAATSKRFFESKGWDPKSIEYLLVGSTV
CCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHCCCCCCCCEEEEEECCHH
YQKQWFYSGPWAAALMGAEATPGILVSQACSTSTFSIYQAGMGVETGMFENSWCLMADRL
HHHCCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEEHHHCCCCCCCCCCCCCEEEEEHHC
SNGPHAAWPNPAGPGGQQIAEDWVMDNFGRDPWAGEAMIQTAENVAKEYGVTREECDALT
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
LRRQEQYLDSLANDREFQKRYLFPAQVQLSKKKSTLLEADEGVVTSTREGLASLNPVLPG
HHHHHHHHHHHHCCHHHHHHCCCCHHEEEHHHHHHHHHCCCCCEECHHHHHHHCCCCCCC
GVHTFGAQTHPADGNAGICVTTREKARELSADPGVVVQLVSFGFARTKKAHMAAAVAPSA
CCEECCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QMALNGAGIKASDLGAVKTHNPFAANDIVMAKVMGLDLDSMNNYGSSLIFGHPQAATGAR
HEEECCCCCCCCCCCCEECCCCCCCCCEEEEHHHCCCHHHHCCCCCEEEECCCCCCCHHH
LIVEGIEELAQKGGGYLLFSGCAAGDTAASLVLKVN
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEC
>Mature Secondary Structure
MLKKAYIPYRGYYSTPFAKWQGTLANEHSIVFGAATSKRFFESKGWDPKSIEYLLVGSTV
CCCCCCCCCCCCCCCCHHHHCCCCCCCCEEEEECCCCHHHHHCCCCCCCCEEEEEECCHH
YQKQWFYSGPWAAALMGAEATPGILVSQACSTSTFSIYQAGMGVETGMFENSWCLMADRL
HHHCCCCCCCHHHHHCCCCCCCCCEEECCCCCCEEEHHHCCCCCCCCCCCCCEEEEEHHC
SNGPHAAWPNPAGPGGQQIAEDWVMDNFGRDPWAGEAMIQTAENVAKEYGVTREECDALT
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHH
LRRQEQYLDSLANDREFQKRYLFPAQVQLSKKKSTLLEADEGVVTSTREGLASLNPVLPG
HHHHHHHHHHHHCCHHHHHHCCCCHHEEEHHHHHHHHHCCCCCEECHHHHHHHCCCCCCC
GVHTFGAQTHPADGNAGICVTTREKARELSADPGVVVQLVSFGFARTKKAHMAAAVAPSA
CCEECCCCCCCCCCCCEEEEEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
QMALNGAGIKASDLGAVKTHNPFAANDIVMAKVMGLDLDSMNNYGSSLIFGHPQAATGAR
HEEECCCCCCCCCCCCEECCCCCCCCCEEEEHHHCCCHHHHCCCCCEEEECCCCCCCHHH
LIVEGIEELAQKGGGYLLFSGCAAGDTAASLVLKVN
HHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]