BacMap

Definition	Geobacter bemidjiensis Bem chromosome, complete genome.
Accession	NC_011146
Length	4,615,150

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The map label for this gene is paaF [C]

Identifier: 197117804

GI number: 197117804

Start: 1633196

End: 1633978

Strand: Direct

Name: paaF [C]

Synonym: Gbem_1416

Alternate gene names: 197117804

Gene position: 1633196-1633978 (Clockwise)

Preceding gene: 197117803

Following gene: 197117805

Centisome position: 35.39

GC content: 64.75

Gene sequence:

>783_bases
ATGGGCTATCAGTTCCTCAATATGAACCGGGAAGACCGGGTCCTCACCATCAACTTGAACCGTCCCCCCACCAACCCGCT
CAGTCGCGGCTTCGGCGAGGAGCTGCTCAAGGCCTTCACCGAGGCGGAGGGGATGGACGACGTGAACGTGGTGGTCATCA
CCAGCGCGCTGGAGAAGGCTTTCATCGCCGGCGCCGACATCAAGGAGATGTCCGCCATGGGGCAGGCCGAATCCGAGGCC
TTCTCGAAGCTGTTGCAAGACGCGAACAACACCCTCGACCGGATGAAGAAGGTGGTCATCGCCGCCATCAACGGCCACGC
CCTGGGAGGGGGGTGCGAACTCGCCATGGCGTGCGACTACCGCTTCATGGCGGCGGGGAAGGCGCTGGTCGGCCTTCCTG
AGGCCGGGCTCGGCATCGTCCCCGGGGCGGGGGGAACCCAGCGGCTGCCGCGCCTGGTGGGGCTTGCCAAGGCGAAAGAC
ATCCTTTTGTGGGGCAAGGTGATGGGGCCGGAGGAGGCGCTAGCCATTGGGCTCGTGGACCGGGTGATCCCGGCGGAGAG
CTTCCTCGACGAGGTGATGGAATTCGCCCACCGGCTCGCCTCCGGCGCCGGCAAGGCGCTTGGCTTCATCAAGGTGGCGG
TCAACGAAGCGGTGGACCTCCCCATGGAACAGGCGCTGGCGGTGGAGCGCAAATACGGCCTGGCGAACCTGCTCACCCAC
GACGCGAAGGAAGGGCTCACCGCCTTCGGCGAGAAGAGAAAACCGAACTTTTTAAGCAGATAA

Upstream 100 bases:

>100_bases
GGGCGGAATGAAGCGTTCCGCCAGAGGGACAGGCACCTACGGAGCCAGTCCCTTCCGCCCCCGCCCGAAGCCATCAACCA
TTTTCAAAGGGAGCGAAACC

Downstream 100 bases:

>100_bases
CCTCAACGATTTCGATAACGAGACCAACTCACTACGATTGCAAAAAGGAGGATACGCATGAACGTCGAGGATATCACCAC
AATGGGAATGGCAGGCGCCG

Product: enoyl-CoA hydratase/isomerase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MGYQFLNMNREDRVLTINLNRPPTNPLSRGFGEELLKAFTEAEGMDDVNVVVITSALEKAFIAGADIKEMSAMGQAESEA
FSKLLQDANNTLDRMKKVVIAAINGHALGGGCELAMACDYRFMAAGKALVGLPEAGLGIVPGAGGTQRLPRLVGLAKAKD
ILLWGKVMGPEEALAIGLVDRVIPAESFLDEVMEFAHRLASGAGKALGFIKVAVNEAVDLPMEQALAVERKYGLANLLTH
DAKEGLTAFGEKRKPNFLSR

Sequences:

>Translated_260_residues
MGYQFLNMNREDRVLTINLNRPPTNPLSRGFGEELLKAFTEAEGMDDVNVVVITSALEKAFIAGADIKEMSAMGQAESEA
FSKLLQDANNTLDRMKKVVIAAINGHALGGGCELAMACDYRFMAAGKALVGLPEAGLGIVPGAGGTQRLPRLVGLAKAKD
ILLWGKVMGPEEALAIGLVDRVIPAESFLDEVMEFAHRLASGAGKALGFIKVAVNEAVDLPMEQALAVERKYGLANLLTH
DAKEGLTAFGEKRKPNFLSR
>Mature_259_residues
GYQFLNMNREDRVLTINLNRPPTNPLSRGFGEELLKAFTEAEGMDDVNVVVITSALEKAFIAGADIKEMSAMGQAESEAF
SKLLQDANNTLDRMKKVVIAAINGHALGGGCELAMACDYRFMAAGKALVGLPEAGLGIVPGAGGTQRLPRLVGLAKAKDI
LLWGKVMGPEEALAIGLVDRVIPAESFLDEVMEFAHRLASGAGKALGFIKVAVNEAVDLPMEQALAVERKYGLANLLTHD
AKEGLTAFGEKRKPNFLSR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=40.5622489959839, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI4502327, Length=257, Percent_Identity=34.2412451361868, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI68989263, Length=184, Percent_Identity=38.5869565217391, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI20127408, Length=212, Percent_Identity=33.4905660377358, Blast_Score=113, Evalue=1e-25,
Organism=Homo sapiens, GI150378541, Length=252, Percent_Identity=30.1587301587302, Blast_Score=101, Evalue=8e-22,
Organism=Homo sapiens, GI70995211, Length=258, Percent_Identity=29.0697674418605, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI62530384, Length=174, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=8e-19,
Organism=Homo sapiens, GI157694516, Length=232, Percent_Identity=27.1551724137931, Blast_Score=84, Evalue=9e-17,
Organism=Homo sapiens, GI213417737, Length=232, Percent_Identity=27.1551724137931, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31542718, Length=251, Percent_Identity=27.0916334661355, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI261878539, Length=94, Percent_Identity=45.7446808510638, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI157694520, Length=199, Percent_Identity=27.6381909547739, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI295842266, Length=173, Percent_Identity=30.635838150289, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI221307494, Length=184, Percent_Identity=30.9782608695652, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=231, Percent_Identity=27.7056277056277, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI221136756, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI221136753, Length=184, Percent_Identity=30.9782608695652, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI25453481, Length=229, Percent_Identity=28.3842794759825, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI51538573, Length=229, Percent_Identity=28.3842794759825, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI4757966, Length=229, Percent_Identity=28.3842794759825, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI51538581, Length=229, Percent_Identity=28.3842794759825, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4757968, Length=231, Percent_Identity=27.7056277056277, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI49355787, Length=231, Percent_Identity=27.7056277056277, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1787659, Length=263, Percent_Identity=37.2623574144487, Blast_Score=141, Evalue=4e-35,
Organism=Escherichia coli, GI221142681, Length=259, Percent_Identity=35.9073359073359, Blast_Score=137, Evalue=9e-34,
Organism=Escherichia coli, GI1788682, Length=187, Percent_Identity=38.5026737967914, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790281, Length=134, Percent_Identity=45.5223880597015, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788597, Length=252, Percent_Identity=29.3650793650794, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI87082183, Length=261, Percent_Identity=25.6704980842912, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1787660, Length=263, Percent_Identity=30.0380228136882, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI25145438, Length=252, Percent_Identity=38.8888888888889, Blast_Score=158, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17554946, Length=251, Percent_Identity=39.8406374501992, Blast_Score=154, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17540714, Length=233, Percent_Identity=35.1931330472103, Blast_Score=140, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI17558304, Length=208, Percent_Identity=35.5769230769231, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI25144276, Length=204, Percent_Identity=36.2745098039216, Blast_Score=108, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17508953, Length=204, Percent_Identity=36.2745098039216, Blast_Score=107, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17508951, Length=204, Percent_Identity=36.2745098039216, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=27.7566539923954, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=267, Percent_Identity=26.9662921348315, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17560910, Length=255, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17549921, Length=182, Percent_Identity=31.8681318681319, Blast_Score=77, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=192, Percent_Identity=31.25, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17540306, Length=253, Percent_Identity=28.8537549407115, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320241, Length=188, Percent_Identity=27.1276595744681, Blast_Score=65, Evalue=7e-12,
Organism=Drosophila melanogaster, GI20129971, Length=242, Percent_Identity=41.7355371900826, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653477, Length=242, Percent_Identity=41.7355371900826, Blast_Score=176, Evalue=2e-44,
Organism=Drosophila melanogaster, GI24653139, Length=249, Percent_Identity=34.9397590361446, Blast_Score=130, Evalue=8e-31,
Organism=Drosophila melanogaster, GI21357171, Length=255, Percent_Identity=29.0196078431373, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19922422, Length=245, Percent_Identity=27.7551020408163, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19921000, Length=206, Percent_Identity=36.8932038834951, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24583077, Length=206, Percent_Identity=36.8932038834951, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583079, Length=206, Percent_Identity=36.8932038834951, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24583165, Length=173, Percent_Identity=33.5260115606936, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921018, Length=210, Percent_Identity=31.9047619047619, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24650670, Length=249, Percent_Identity=28.5140562248996, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=213, Percent_Identity=29.1079812206573, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24654903, Length=192, Percent_Identity=26.5625, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550169, Length=174, Percent_Identity=28.735632183908, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28571729, Length=140, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI28571730, Length=140, Percent_Identity=32.1428571428571, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27784; Mature: 27652

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYQFLNMNREDRVLTINLNRPPTNPLSRGFGEELLKAFTEAEGMDDVNVVVITSALEKA
CCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
FIAGADIKEMSAMGQAESEAFSKLLQDANNTLDRMKKVVIAAINGHALGGGCELAMACDY
HHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCC
RFMAAGKALVGLPEAGLGIVPGAGGTQRLPRLVGLAKAKDILLWGKVMGPEEALAIGLVD
HHHHCCCHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
RVIPAESFLDEVMEFAHRLASGAGKALGFIKVAVNEAVDLPMEQALAVERKYGLANLLTH
HHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHH
DAKEGLTAFGEKRKPNFLSR
HHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GYQFLNMNREDRVLTINLNRPPTNPLSRGFGEELLKAFTEAEGMDDVNVVVITSALEKA
CCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHH
FIAGADIKEMSAMGQAESEAFSKLLQDANNTLDRMKKVVIAAINGHALGGGCELAMACDY
HHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEEEECCC
RFMAAGKALVGLPEAGLGIVPGAGGTQRLPRLVGLAKAKDILLWGKVMGPEEALAIGLVD
HHHHCCCHHCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHH
RVIPAESFLDEVMEFAHRLASGAGKALGFIKVAVNEAVDLPMEQALAVERKYGLANLLTH
HHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCHHHHHHH
DAKEGLTAFGEKRKPNFLSR
HHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA