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The map label for this gene is cmoB

Identifier: 197117790

GI number: 197117790

Start: 1615701

End: 1616672

Strand: Reverse

Name: cmoB

Synonym: Gbem_1402

Alternate gene names: 197117790

Gene position: 1616672-1615701 (Counterclockwise)

Preceding gene: 197117791

Following gene: 197117789

Centisome position: 35.03

GC content: 61.73

Gene sequence:

>972_bases
ATGAGCAACTACGACGCCCTCTATCGCCAGCTAGCCGCCATGGGTCAGGAACGCTGGGCAGAGCAGCTGCAAGCGACCCT
GCCGGATAAACTGGCCCTCGAAAGTACCGCCAAGATGGCCGGCTGGCAAAGCGCCATGCAATCGCTGCCGGAAATAAGCC
CGTCCAGAATCGAGCTGCTGGATACCGTCACCATCGGCAGCAGCGACGACCTCGGCCAAGTCGACCGCGAAGAGCTGATG
GCGCACCTCCAGGCCTTTCACCCCTGGCGCAAAGGCCCCTACAATTTCTTCGGCATCGAGATCGATACCGAGTGGCGCTC
CGACTGGAAATGGGAGCGGCTGCTGCCGCACATTCAACCGCTGGCGGGACGCAGAGTCATCGACGTCGGCTGCGGCAACG
GCTATCACGGCTGGCGCATGCGCGGCGCCGGCGCCGAGTTCGTCCTCGGCATCGAGCCCTTCCTGCTTTCGGTGCAGCAG
TTCCAGGTGATGCAACGCTACCTTTGCGATCCGCAGCATCATGTCATCCCCATCGGCATCGAGGAGGTCCCCCCTAACCT
CGCCTGCTTCGACAGCGTCTTCTCCATGGGCGTCCTTTACCATCGCCGCTCCCCGCTGGACCATCTTTTCGAGCTGAAAG
GGTGTCTGCGCCCGGGTGGGGAATTGATTCTGGAGACGCTGATTGTGGAAGGGGATCGGGAGACCATCTTCATGCCGCCG
GGACGTTACGCCAAGATGCGCAACGTCTGGTTCATACCGTCCATTGCGGCGATGACCTTGTGGCTTGAGCGCTGCGGGTT
CACCGACATTGCCTGCGTCGACACCAACCGTACCAGCCGCGAAGAGCAGCGCTCCACCGAGTGGATGCGCTTTGAATCGC
TGGCCGATTTTCTTGATCCGGACGATGCACAGAAGACGATTGAAGGGCACCCCGCGCCGCTAAGGGCGATCTTTACGGCG
ACGAAGCCGTAG

Upstream 100 bases:

>100_bases
AGACCGTCGCCTGCCACCAGGAGCGGCTGCTGCAGGCTGGCTTCTCCTCCTCCGAGCTTTGGTTCCAGTGCTTCAACTTC
GCCTCCATGGTCGCATTTAA

Downstream 100 bases:

>100_bases
CAACCGGGCCATGAAACAGCCGACAGTGCCCGGGGGCAGAGCTTGTCCACCTCAGTTTTGTTTGTCGGGGAGAATCGGGT
TATACTTCGGGAGAAGATTA

Product: tRNA (5-methoxy-U34)-methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSNYDALYRQLAAMGQERWAEQLQATLPDKLALESTAKMAGWQSAMQSLPEISPSRIELLDTVTIGSSDDLGQVDREELM
AHLQAFHPWRKGPYNFFGIEIDTEWRSDWKWERLLPHIQPLAGRRVIDVGCGNGYHGWRMRGAGAEFVLGIEPFLLSVQQ
FQVMQRYLCDPQHHVIPIGIEEVPPNLACFDSVFSMGVLYHRRSPLDHLFELKGCLRPGGELILETLIVEGDRETIFMPP
GRYAKMRNVWFIPSIAAMTLWLERCGFTDIACVDTNRTSREEQRSTEWMRFESLADFLDPDDAQKTIEGHPAPLRAIFTA
TKP

Sequences:

>Translated_323_residues
MSNYDALYRQLAAMGQERWAEQLQATLPDKLALESTAKMAGWQSAMQSLPEISPSRIELLDTVTIGSSDDLGQVDREELM
AHLQAFHPWRKGPYNFFGIEIDTEWRSDWKWERLLPHIQPLAGRRVIDVGCGNGYHGWRMRGAGAEFVLGIEPFLLSVQQ
FQVMQRYLCDPQHHVIPIGIEEVPPNLACFDSVFSMGVLYHRRSPLDHLFELKGCLRPGGELILETLIVEGDRETIFMPP
GRYAKMRNVWFIPSIAAMTLWLERCGFTDIACVDTNRTSREEQRSTEWMRFESLADFLDPDDAQKTIEGHPAPLRAIFTA
TKP
>Mature_322_residues
SNYDALYRQLAAMGQERWAEQLQATLPDKLALESTAKMAGWQSAMQSLPEISPSRIELLDTVTIGSSDDLGQVDREELMA
HLQAFHPWRKGPYNFFGIEIDTEWRSDWKWERLLPHIQPLAGRRVIDVGCGNGYHGWRMRGAGAEFVLGIEPFLLSVQQF
QVMQRYLCDPQHHVIPIGIEEVPPNLACFDSVFSMGVLYHRRSPLDHLFELKGCLRPGGELILETLIVEGDRETIFMPPG
RYAKMRNVWFIPSIAAMTLWLERCGFTDIACVDTNRTSREEQRSTEWMRFESLADFLDPDDAQKTIEGHPAPLRAIFTAT
KP

Specific function: Catalyzes the conversion of 5-hydroxyuridine (ho5U) to 5-methoxyuridine (mo5U) at position 34 in tRNA

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. CmoB family

Homologues:

Organism=Escherichia coli, GI1788178, Length=326, Percent_Identity=50, Blast_Score=333, Evalue=9e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CMOB_GEOBB (B5EIQ6)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138217.1
- ProteinModelPortal:   B5EIQ6
- SMR:   B5EIQ6
- GeneID:   6781389
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1402
- HOGENOM:   HBG296882
- OMA:   DVIFCLG
- ProtClustDB:   PRK15068
- HAMAP:   MF_01590
- InterPro:   IPR010017
- TIGRFAMs:   TIGR00452

Pfam domain/function: PF08003 Methyltransf_9

EC number: NA

Molecular weight: Translated: 36908; Mature: 36776

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNYDALYRQLAAMGQERWAEQLQATLPDKLALESTAKMAGWQSAMQSLPEISPSRIELL
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DTVTIGSSDDLGQVDREELMAHLQAFHPWRKGPYNFFGIEIDTEWRSDWKWERLLPHIQP
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
LAGRRVIDVGCGNGYHGWRMRGAGAEFVLGIEPFLLSVQQFQVMQRYLCDPQHHVIPIGI
HCCCEEEEECCCCCCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
EEVPPNLACFDSVFSMGVLYHRRSPLDHLFELKGCLRPGGELILETLIVEGDRETIFMPP
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECC
GRYAKMRNVWFIPSIAAMTLWLERCGFTDIACVDTNRTSREEQRSTEWMRFESLADFLDP
CCCHHHCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DDAQKTIEGHPAPLRAIFTATKP
CCHHHHHCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SNYDALYRQLAAMGQERWAEQLQATLPDKLALESTAKMAGWQSAMQSLPEISPSRIELL
CCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
DTVTIGSSDDLGQVDREELMAHLQAFHPWRKGPYNFFGIEIDTEWRSDWKWERLLPHIQP
EEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHH
LAGRRVIDVGCGNGYHGWRMRGAGAEFVLGIEPFLLSVQQFQVMQRYLCDPQHHVIPIGI
HCCCEEEEECCCCCCCCCEEECCCCCEEEECHHHHHHHHHHHHHHHHHCCCCCCEEEEEH
EEVPPNLACFDSVFSMGVLYHRRSPLDHLFELKGCLRPGGELILETLIVEGDRETIFMPP
HHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECC
GRYAKMRNVWFIPSIAAMTLWLERCGFTDIACVDTNRTSREEQRSTEWMRFESLADFLDP
CCCHHHCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
DDAQKTIEGHPAPLRAIFTATKP
CCHHHHHCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA