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The map label for this gene is hyaL [H]

Identifier: 197117756

GI number: 197117756

Start: 1575041

End: 1576756

Strand: Reverse

Name: hyaL [H]

Synonym: Gbem_1368

Alternate gene names: 197117756

Gene position: 1576756-1575041 (Counterclockwise)

Preceding gene: 197117757

Following gene: 197117755

Centisome position: 34.16

GC content: 66.32

Gene sequence:

>1716_bases
ATGGCAACAGAGCGCATCGTCATTGACCCGATCACCAGGATCGAGGGGCACCTGAGAATAGAGCTTGAGGCGGAGGGGGG
GAGAATCGGCAACGCCTGGGCCTGCGCCACCCAGTTCCGGGGAATCGAAAAGATCCTCGAAGGACGGGACCCGCGAGACG
CCTGGGCTTTCGCGCAGCGCATCTGCGGGGTCTGCACCGGCGTCCACGCCATCGCCTCGATACGGGCCGTCGAGGACGCC
ATCAAATGCCGCATCCCCGAGTCGGCAGAGTTGATCCGGAACCTGGTCTCCGGGATGGCGACCCTACAGGACCACGTCAT
GCATTTCTACCACCTGCACGCCCTTGACTGGGTGGACGTGATGAGCGCGCTTGCCGCCGACCCAGCCGCCACCTCGCGCC
TTGCCGCCTCCGTCTCCCCCTGGCCCAACAACTCCGCCTCCTGGTTCAAGGAGGTGCAGCAGCGGGTCGCGACCTCGGCC
AAGGGAGGGCTCGGGATCTTCGCTTCCGGCTACTGGGGGCACCCCGCCTACCGCCTCCCTCCCGAGGCGAACCTGATGGC
GGTCGCCCACTACCTGGAGGCGCTCAAGTGGCAGCGCGAGATCATCAAGCTCCACACCATCTTCGCCGGGAAGAACCCCC
ACCCCAACTACCTGGTGGGGGGGATGGCGACCTCCATCAACCTGGACAACCAGCTCACCATCAACAAGGTGCGTCTGGAC
CAGTTGGGGGAACTGATCCGGGAAGCACGCCGCTTCGTCGACGAGGTGTACTACCCCGACGTCATGGCCATCGCCGGCTT
CTACCCCGAATACGCCTCCATCGGCGTCTCCTCCCCCACCCTCATGGCCGTAGGCGAGAGCGCCTTCTCCTGCGCCGGCA
CGCCGGCCGGAAAGCCGGTCGCAGCAGGTCTCCTCATGGACGGCGACTACCGAACCTCACACCCTTTCAACCCCAGGATG
GTCGCCGAGTACGTCTCCTCCGCCTGGTACCTCTATCCGGAGGGGGACCGGCAGGGGCTGCACCCGTGGCAGGGTAGGAC
GGAGCCCAACTACACCGGTCCCAAGCCCCCCTACCGCCAGCTTTCCGACCAGGAGAAGTACACCTGGTGCAAGGCGCCGC
GCTACGACGGCCGCCCGGTCCAGGTCGGCCCCAACGCGAGGATCATGCTGGCCTACGCCCAGGGGCACCCAGATACGGTG
CAGCTCGCCAACCGGGACCTGGGAAAACTGAAAGCCGGGGTCGAGGCGCTCAATTCCACGCTCGGGCGCACCTACTGCCG
CGCCCTGGAATCGGTCATCCTCGCCCGGCGCATGGAGGAATGGTTCGCGGCCTTGAACGAGCGGATCAGGTCCGGCGACG
TCTCCACCTTCAACGCCGAACTCTGGGAGCCGGAAAGCTGGCCCAAACAAGCGCAGGGAATGGGTTACCTGGAGGCCGCG
CGCGGGACGCTCTCGCACTGGGTGGAGATAGAAAACGGGCGCATCGCCCGCTACCAGTGCGTGGTTCCCAGCACCTGGAA
CAGCTCGGGGCGCGATCCCAACGGCCTGATGGGCCCCTACGAGCAGGCCCTGGCGGGGGACAAGAAGCATCCGCTGGTCG
ACCCCAAGCGCCCCGTCGAGGTGTTGCGCACCATACACTCCTTCGACCCGTGCCTATCCTGCGCCGTGCATCTGCTGGTG
CCGGGGGGGCCGGCGTTTGAGGTGAAAGTGCGATGA

Upstream 100 bases:

>100_bases
GCCGAGCCGGAAAGCCTGCCGCTGGCGGTACTGGGAGACAGGAAGGAAGCTGACGAGAAGGAAAAGGATTAGGCCTTCCC
GAGCGGCAAGGAGAAACCAG

Downstream 100 bases:

>100_bases
AGATCTTCGACATCCCCGAGGACGAGATCCGCATCGAGCTGGGGGACCCTGCCCCCTTCGACGGCGGGAAACGGCGCGAG
CTGATGCTGGCCGCGGCGCT

Product: [NiFe]-hydrogenase large subunit

Products: NA

Alternate protein names: Hydrogenlyase; Membrane-bound hydrogenase large subunit [H]

Number of amino acids: Translated: 571; Mature: 570

Protein sequence:

>571_residues
MATERIVIDPITRIEGHLRIELEAEGGRIGNAWACATQFRGIEKILEGRDPRDAWAFAQRICGVCTGVHAIASIRAVEDA
IKCRIPESAELIRNLVSGMATLQDHVMHFYHLHALDWVDVMSALAADPAATSRLAASVSPWPNNSASWFKEVQQRVATSA
KGGLGIFASGYWGHPAYRLPPEANLMAVAHYLEALKWQREIIKLHTIFAGKNPHPNYLVGGMATSINLDNQLTINKVRLD
QLGELIREARRFVDEVYYPDVMAIAGFYPEYASIGVSSPTLMAVGESAFSCAGTPAGKPVAAGLLMDGDYRTSHPFNPRM
VAEYVSSAWYLYPEGDRQGLHPWQGRTEPNYTGPKPPYRQLSDQEKYTWCKAPRYDGRPVQVGPNARIMLAYAQGHPDTV
QLANRDLGKLKAGVEALNSTLGRTYCRALESVILARRMEEWFAALNERIRSGDVSTFNAELWEPESWPKQAQGMGYLEAA
RGTLSHWVEIENGRIARYQCVVPSTWNSSGRDPNGLMGPYEQALAGDKKHPLVDPKRPVEVLRTIHSFDPCLSCAVHLLV
PGGPAFEVKVR

Sequences:

>Translated_571_residues
MATERIVIDPITRIEGHLRIELEAEGGRIGNAWACATQFRGIEKILEGRDPRDAWAFAQRICGVCTGVHAIASIRAVEDA
IKCRIPESAELIRNLVSGMATLQDHVMHFYHLHALDWVDVMSALAADPAATSRLAASVSPWPNNSASWFKEVQQRVATSA
KGGLGIFASGYWGHPAYRLPPEANLMAVAHYLEALKWQREIIKLHTIFAGKNPHPNYLVGGMATSINLDNQLTINKVRLD
QLGELIREARRFVDEVYYPDVMAIAGFYPEYASIGVSSPTLMAVGESAFSCAGTPAGKPVAAGLLMDGDYRTSHPFNPRM
VAEYVSSAWYLYPEGDRQGLHPWQGRTEPNYTGPKPPYRQLSDQEKYTWCKAPRYDGRPVQVGPNARIMLAYAQGHPDTV
QLANRDLGKLKAGVEALNSTLGRTYCRALESVILARRMEEWFAALNERIRSGDVSTFNAELWEPESWPKQAQGMGYLEAA
RGTLSHWVEIENGRIARYQCVVPSTWNSSGRDPNGLMGPYEQALAGDKKHPLVDPKRPVEVLRTIHSFDPCLSCAVHLLV
PGGPAFEVKVR
>Mature_570_residues
ATERIVIDPITRIEGHLRIELEAEGGRIGNAWACATQFRGIEKILEGRDPRDAWAFAQRICGVCTGVHAIASIRAVEDAI
KCRIPESAELIRNLVSGMATLQDHVMHFYHLHALDWVDVMSALAADPAATSRLAASVSPWPNNSASWFKEVQQRVATSAK
GGLGIFASGYWGHPAYRLPPEANLMAVAHYLEALKWQREIIKLHTIFAGKNPHPNYLVGGMATSINLDNQLTINKVRLDQ
LGELIREARRFVDEVYYPDVMAIAGFYPEYASIGVSSPTLMAVGESAFSCAGTPAGKPVAAGLLMDGDYRTSHPFNPRMV
AEYVSSAWYLYPEGDRQGLHPWQGRTEPNYTGPKPPYRQLSDQEKYTWCKAPRYDGRPVQVGPNARIMLAYAQGHPDTVQ
LANRDLGKLKAGVEALNSTLGRTYCRALESVILARRMEEWFAALNERIRSGDVSTFNAELWEPESWPKQAQGMGYLEAAR
GTLSHWVEIENGRIARYQCVVPSTWNSSGRDPNGLMGPYEQALAGDKKHPLVDPKRPVEVLRTIHSFDPCLSCAVHLLVP
GGPAFEVKVR

Specific function: This enzyme recycles the H(2) produced by nitrogenase to increase the production of ATP and to protect nitrogenase against inhibition or damage by O(2) under carbon- or phosphate-limited conditions [H]

COG id: COG0374

COG function: function code C; Ni,Fe-hydrogenase I large subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

Organism=Escherichia coli, GI1787207, Length=587, Percent_Identity=45.6558773424191, Blast_Score=541, Evalue=1e-155,
Organism=Escherichia coli, GI1789368, Length=584, Percent_Identity=42.4657534246575, Blast_Score=446, Evalue=1e-126,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.99.6 [H]

Molecular weight: Translated: 63268; Mature: 63137

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00507 NI_HGENASE_L_1 ; PS00508 NI_HGENASE_L_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATERIVIDPITRIEGHLRIELEAEGGRIGNAWACATQFRGIEKILEGRDPRDAWAFAQR
CCCCCEEECCHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ICGVCTGVHAIASIRAVEDAIKCRIPESAELIRNLVSGMATLQDHVMHFYHLHALDWVDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSALAADPAATSRLAASVSPWPNNSASWFKEVQQRVATSAKGGLGIFASGYWGHPAYRLP
HHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
PEANLMAVAHYLEALKWQREIIKLHTIFAGKNPHPNYLVGGMATSINLDNQLTINKVRLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECCEEEEECCCCCEEEHHHHHH
QLGELIREARRFVDEVYYPDVMAIAGFYPEYASIGVSSPTLMAVGESAFSCAGTPAGKPV
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCCCCEEEECCCHHHCCCCCCCCCC
AAGLLMDGDYRTSHPFNPRMVAEYVSSAWYLYPEGDRQGLHPWQGRTEPNYTGPKPPYRQ
EEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
LSDQEKYTWCKAPRYDGRPVQVGPNARIMLAYAQGHPDTVQLANRDLGKLKAGVEALNST
CCCCCCCEEECCCCCCCCCEEECCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHH
LGRTYCRALESVILARRMEEWFAALNERIRSGDVSTFNAELWEPESWPKQAQGMGYLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCHHHHH
RGTLSHWVEIENGRIARYQCVVPSTWNSSGRDPNGLMGPYEQALAGDKKHPLVDPKRPVE
HHHHHHEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHH
VLRTIHSFDPCLSCAVHLLVPGGPAFEVKVR
HHHHHHHCCHHHHHHHHHEECCCCCEEEEEC
>Mature Secondary Structure 
ATERIVIDPITRIEGHLRIELEAEGGRIGNAWACATQFRGIEKILEGRDPRDAWAFAQR
CCCCEEECCHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
ICGVCTGVHAIASIRAVEDAIKCRIPESAELIRNLVSGMATLQDHVMHFYHLHALDWVDV
HHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSALAADPAATSRLAASVSPWPNNSASWFKEVQQRVATSAKGGLGIFASGYWGHPAYRLP
HHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCC
PEANLMAVAHYLEALKWQREIIKLHTIFAGKNPHPNYLVGGMATSINLDNQLTINKVRLD
CCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCEEECCEEEEECCCCCEEEHHHHHH
QLGELIREARRFVDEVYYPDVMAIAGFYPEYASIGVSSPTLMAVGESAFSCAGTPAGKPV
HHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHCCCCCCCEEEECCCHHHCCCCCCCCCC
AAGLLMDGDYRTSHPFNPRMVAEYVSSAWYLYPEGDRQGLHPWQGRTEPNYTGPKPPYRQ
EEEEEECCCCCCCCCCCHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHH
LSDQEKYTWCKAPRYDGRPVQVGPNARIMLAYAQGHPDTVQLANRDLGKLKAGVEALNST
CCCCCCCEEECCCCCCCCCEEECCCCEEEEEEECCCCCCHHHHCCCHHHHHHHHHHHHHH
LGRTYCRALESVILARRMEEWFAALNERIRSGDVSTFNAELWEPESWPKQAQGMGYLEAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHCCCCHHHHH
RGTLSHWVEIENGRIARYQCVVPSTWNSSGRDPNGLMGPYEQALAGDKKHPLVDPKRPVE
HHHHHHEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHH
VLRTIHSFDPCLSCAVHLLVPGGPAFEVKVR
HHHHHHHCCHHHHHHHHHEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3054886; 12597275; 8163174 [H]