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The map label for this gene is hyaB [H]

Identifier: 197117755

GI number: 197117755

Start: 1573872

End: 1575044

Strand: Reverse

Name: hyaB [H]

Synonym: Gbem_1367

Alternate gene names: 197117755

Gene position: 1575044-1573872 (Counterclockwise)

Preceding gene: 197117756

Following gene: 197117754

Centisome position: 34.13

GC content: 64.62

Gene sequence:

>1173_bases
ATGAAGATCTTCGACATCCCCGAGGACGAGATCCGCATCGAGCTGGGGGACCCTGCCCCCTTCGACGGCGGGAAACGGCG
CGAGCTGATGCTGGCCGCGGCGCTCGGCGCCCCGGTCGGCACCCGGGACCCGGTCGACCTCGCCTTGTTGAGCGCGGCCT
CGCGCAAAGAGGACCTGCGGCACTTTGAGCAGACGGCGTTCACGCCGCTGGAGCCGCGACTGGCGCGGAGCATCGCGCGG
GTGCGCCGGGTGGGAACGGAGGAGGAAGAGTTGATCGCACGCGGCGAGGTCGACGCCATCCTGTACCTCTGCCGCGCCGA
CGAGGCGACCCGCTTCCGGGCGGAGTTGCAGGCCGAGATGAGGATGACCCGCGGCTACCGCGCGCTCGGGATCGCCAAGG
CTAAGCCGGAGCCGAAGGGGAACGAGAGCTGGACCTTCATGGGGTACATCCCGGTCCGCGCCACGCGGCATAAAAGCACG
CACAGCGAAGAGCCCGCCGACTTCAACTACGTCACCGTCTGGGACTGGCAACTGCGCGTACTGCACTGGCTTTCGGTCTT
CCTGATACTGGTGCTGTCGCTCACGGGCCTCTTAATGGGGAGCAGCCGGTTCATCTACGGCGTTACGGAAGGGTATTCCA
ACTACCTGAGCTGGCTCAGGCTCACCCACTTCGTCGCGGGGTGGTTCCTTCTTTGCGCCGCCATCCTGCGCATAGCCGGG
CTCTTTCTCGCCTCCAACCGCTTTCAGCGCTGGTACGCCCTCTTCCCGGTGAAAAAGCGCGACCTGAACAACCTGGTACA
GGTGGTGCAGAACTACCTCTTCTGCCGTTTCGAGCGCCCCCCCCACTACATCGGCCACAACCCGCTGCAGCAGATCGCCT
ATACCGCCATCTTCGGCGTCGGCCTCGCCGCACTTTTCACCGGGTTCGCGCTCTACGCGCTCTACGCACCGGACCATTGG
CTTTTGCGCTACTTCGTCTGGTTCGACGATCTGTTCGGCGTGCAGTACCTGCGCCTTGCCCACCAACTGATCATGTGGAT
CTTCCTCGCCTTCATCCCGATCCACGTCTACCTCTCCATCCGCGCCGATACGGTGGAGCGCGAGGGGGCTCTCTCCTCCA
TCGTCAGCGGCGGCCGCTGGTGCCGGAAAGGAACAAAATTCGAGGACGGTTAG

Upstream 100 bases:

>100_bases
CCGTCGAGGTGTTGCGCACCATACACTCCTTCGACCCGTGCCTATCCTGCGCCGTGCATCTGCTGGTGCCGGGGGGGCCG
GCGTTTGAGGTGAAAGTGCG

Downstream 100 bases:

>100_bases
ATATGGGAACCGGTGTCAGGTACCTGGGAAGAATCGCCTCGACGGCGGTTATAGCGCTGCTATTGTGCGCTGCGTCAGCC
GGCGCCGCCAAGCTCCGCTC

Product: [NiFe]-hydrogenase b-type cytochrome subunit

Products: oxidized ferredoxin; H2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 390; Mature: 390

Protein sequence:

>390_residues
MKIFDIPEDEIRIELGDPAPFDGGKRRELMLAAALGAPVGTRDPVDLALLSAASRKEDLRHFEQTAFTPLEPRLARSIAR
VRRVGTEEEELIARGEVDAILYLCRADEATRFRAELQAEMRMTRGYRALGIAKAKPEPKGNESWTFMGYIPVRATRHKST
HSEEPADFNYVTVWDWQLRVLHWLSVFLILVLSLTGLLMGSSRFIYGVTEGYSNYLSWLRLTHFVAGWFLLCAAILRIAG
LFLASNRFQRWYALFPVKKRDLNNLVQVVQNYLFCRFERPPHYIGHNPLQQIAYTAIFGVGLAALFTGFALYALYAPDHW
LLRYFVWFDDLFGVQYLRLAHQLIMWIFLAFIPIHVYLSIRADTVEREGALSSIVSGGRWCRKGTKFEDG

Sequences:

>Translated_390_residues
MKIFDIPEDEIRIELGDPAPFDGGKRRELMLAAALGAPVGTRDPVDLALLSAASRKEDLRHFEQTAFTPLEPRLARSIAR
VRRVGTEEEELIARGEVDAILYLCRADEATRFRAELQAEMRMTRGYRALGIAKAKPEPKGNESWTFMGYIPVRATRHKST
HSEEPADFNYVTVWDWQLRVLHWLSVFLILVLSLTGLLMGSSRFIYGVTEGYSNYLSWLRLTHFVAGWFLLCAAILRIAG
LFLASNRFQRWYALFPVKKRDLNNLVQVVQNYLFCRFERPPHYIGHNPLQQIAYTAIFGVGLAALFTGFALYALYAPDHW
LLRYFVWFDDLFGVQYLRLAHQLIMWIFLAFIPIHVYLSIRADTVEREGALSSIVSGGRWCRKGTKFEDG
>Mature_390_residues
MKIFDIPEDEIRIELGDPAPFDGGKRRELMLAAALGAPVGTRDPVDLALLSAASRKEDLRHFEQTAFTPLEPRLARSIAR
VRRVGTEEEELIARGEVDAILYLCRADEATRFRAELQAEMRMTRGYRALGIAKAKPEPKGNESWTFMGYIPVRATRHKST
HSEEPADFNYVTVWDWQLRVLHWLSVFLILVLSLTGLLMGSSRFIYGVTEGYSNYLSWLRLTHFVAGWFLLCAAILRIAG
LFLASNRFQRWYALFPVKKRDLNNLVQVVQNYLFCRFERPPHYIGHNPLQQIAYTAIFGVGLAALFTGFALYALYAPDHW
LLRYFVWFDDLFGVQYLRLAHQLIMWIFLAFIPIHVYLSIRADTVEREGALSSIVSGGRWCRKGTKFEDG

Specific function: Probable b-type cytochrome [H]

COG id: COG1969

COG function: function code C; Ni,Fe-hydrogenase I cytochrome b subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hupC/hyaC/hydC family [H]

Homologues:

Organism=Escherichia coli, GI1787208, Length=209, Percent_Identity=31.5789473684211, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR000516 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: NA

Molecular weight: Translated: 44775; Mature: 44775

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFDIPEDEIRIELGDPAPFDGGKRRELMLAAALGAPVGTRDPVDLALLSAASRKEDLR
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HFEQTAFTPLEPRLARSIARVRRVGTEEEELIARGEVDAILYLCRADEATRFRAELQAEM
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
RMTRGYRALGIAKAKPEPKGNESWTFMGYIPVRATRHKSTHSEEPADFNYVTVWDWQLRV
HHHCCCHHEEECCCCCCCCCCCCEEEEEEEECEEECCCCCCCCCCCCCCEEEEEHHHHHH
LHWLSVFLILVLSLTGLLMGSSRFIYGVTEGYSNYLSWLRLTHFVAGWFLLCAAILRIAG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLASNRFQRWYALFPVKKRDLNNLVQVVQNYLFCRFERPPHYIGHNPLQQIAYTAIFGV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHH
GLAALFTGFALYALYAPDHWLLRYFVWFDDLFGVQYLRLAHQLIMWIFLAFIPIHVYLSI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
RADTVEREGALSSIVSGGRWCRKGTKFEDG
EHHHHHHHHHHHHHHCCCHHHHCCCCCCCC
>Mature Secondary Structure
MKIFDIPEDEIRIELGDPAPFDGGKRRELMLAAALGAPVGTRDPVDLALLSAASRKEDLR
CCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
HFEQTAFTPLEPRLARSIARVRRVGTEEEELIARGEVDAILYLCRADEATRFRAELQAEM
HHHHHCCCCCCHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
RMTRGYRALGIAKAKPEPKGNESWTFMGYIPVRATRHKSTHSEEPADFNYVTVWDWQLRV
HHHCCCHHEEECCCCCCCCCCCCEEEEEEEECEEECCCCCCCCCCCCCCEEEEEHHHHHH
LHWLSVFLILVLSLTGLLMGSSRFIYGVTEGYSNYLSWLRLTHFVAGWFLLCAAILRIAG
HHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFLASNRFQRWYALFPVKKRDLNNLVQVVQNYLFCRFERPPHYIGHNPLQQIAYTAIFGV
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHEEEECCCCCCCCCCHHHHHHHHHHHHH
GLAALFTGFALYALYAPDHWLLRYFVWFDDLFGVQYLRLAHQLIMWIFLAFIPIHVYLSI
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEE
RADTVEREGALSSIVSGGRWCRKGTKFEDG
EHHHHHHHHHHHHHHCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced ferredoxin; 2 H+ [C]

Specific reaction: Reduced ferredoxin + 2 H+ = 2 oxidized ferredoxin + H2 [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7966281 [H]