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The map label for this gene is truB [H]

Identifier: 197117694

GI number: 197117694

Start: 1509467

End: 1510354

Strand: Direct

Name: truB [H]

Synonym: Gbem_1306

Alternate gene names: 197117694

Gene position: 1509467-1510354 (Clockwise)

Preceding gene: 197117693

Following gene: 197117695

Centisome position: 32.71

GC content: 63.51

Gene sequence:

>888_bases
ATGCTGAACGGCTTTTTTGTGATCGACAAGCCGGCCGGTGTCACCTCGCATGACATCGTGTCCAAGGTGCGTCGCGCCAT
CAACCAGAAAAAAGTGGGGCATACGGGAACGCTCGACCCCTTCGCCACAGGCGTGCTGCCCGTTGCCGTGGGCGAAGGGA
CCAAGGCGATCCAGTTCCTGGACGAGTCGGAGAAGGAGTACCGCGCCGTAATGAGGCTCGGTATCGCCACCGACACCCAG
GATCTCACCGGCCAGGTGCTGGCGGAGCGCGACTGGTCGCAGTTGGAAGCAGCCGACCTGGAGCGGCTGGTGCCGCAGTT
TCTGGGTCTCCAAAAGCAGATGCCTCCCATGTTTTCGGCCATCAAGCAGGGGGGCGTTCCGCTCTACAAGCTGGCCAGAA
AGGGGATCGAGGTGGAGCGCGAGGCGCGCGAGGTGGAAATCCACTCGCTTAGCTTTGACTGGATCCGCCTACCCGAGGCG
TGCTTCACCGTTACCTGCTCGCGCGGCACCTACGTGAGGACCCTTGCCTGCGACATCGGCGAGGCTCTTGGGTGCGGGGC
GCACCTGTTGGAGCTGCGGCGCACAAGGAGCGGGCTTTTCCGCGAAAAAGACGCCATCAGCATAGAGGCGCTGGCTGCGG
CCGCGAACCAGCAGGACCTGATCCTCCCGCTGGACCGGGCGCTCGACCACCTGCGCAGGCTGGAGCTGTCCGAGGCGGGG
GGGAGGAAGGTGCAAAACGGCGTGGTGCCGCTAGCTGCCGATTTCACCGAGGCGACTGGAGAGTTTCAGCAGGGCGAACA
GGTGCGACTCTATCAGGGGGAGCGCTTCGCTGCAGTAGCCGAGTACGACATGCTCAAAGGGCTGCGATTGGCGCGTGTAT
TCAACTAG

Upstream 100 bases:

>100_bases
TTGGCGGGGGCGGGCATCATAACGCCGCCGGCTGCACGGTCAAGGGGACTTTGGCCGAGGTGAAGGCCCTGGTCTTCGAC
CGACTGGAGCAGATGCGCTG

Downstream 100 bases:

>100_bases
TATTGTGCTTTACAGACAGTAGTTTCTATGGTACAAAGTTCGGATGTTTCACCTGCAGCGGCTTGACCTCGGCGCTGGCA
GGAATTATAAACTAGAAGGA

Product: tRNA pseudouridine 55 synthase

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MLNGFFVIDKPAGVTSHDIVSKVRRAINQKKVGHTGTLDPFATGVLPVAVGEGTKAIQFLDESEKEYRAVMRLGIATDTQ
DLTGQVLAERDWSQLEAADLERLVPQFLGLQKQMPPMFSAIKQGGVPLYKLARKGIEVEREAREVEIHSLSFDWIRLPEA
CFTVTCSRGTYVRTLACDIGEALGCGAHLLELRRTRSGLFREKDAISIEALAAAANQQDLILPLDRALDHLRRLELSEAG
GRKVQNGVVPLAADFTEATGEFQQGEQVRLYQGERFAAVAEYDMLKGLRLARVFN

Sequences:

>Translated_295_residues
MLNGFFVIDKPAGVTSHDIVSKVRRAINQKKVGHTGTLDPFATGVLPVAVGEGTKAIQFLDESEKEYRAVMRLGIATDTQ
DLTGQVLAERDWSQLEAADLERLVPQFLGLQKQMPPMFSAIKQGGVPLYKLARKGIEVEREAREVEIHSLSFDWIRLPEA
CFTVTCSRGTYVRTLACDIGEALGCGAHLLELRRTRSGLFREKDAISIEALAAAANQQDLILPLDRALDHLRRLELSEAG
GRKVQNGVVPLAADFTEATGEFQQGEQVRLYQGERFAAVAEYDMLKGLRLARVFN
>Mature_295_residues
MLNGFFVIDKPAGVTSHDIVSKVRRAINQKKVGHTGTLDPFATGVLPVAVGEGTKAIQFLDESEKEYRAVMRLGIATDTQ
DLTGQVLAERDWSQLEAADLERLVPQFLGLQKQMPPMFSAIKQGGVPLYKLARKGIEVEREAREVEIHSLSFDWIRLPEA
CFTVTCSRGTYVRTLACDIGEALGCGAHLLELRRTRSGLFREKDAISIEALAAAANQQDLILPLDRALDHLRRLELSEAG
GRKVQNGVVPLAADFTEATGEFQQGEQVRLYQGERFAAVAEYDMLKGLRLARVFN

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=250, Percent_Identity=34, Blast_Score=137, Evalue=8e-33,
Organism=Homo sapiens, GI4503337, Length=256, Percent_Identity=29.296875, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI215599015, Length=256, Percent_Identity=29.296875, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI2367200, Length=271, Percent_Identity=40.590405904059, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17553978, Length=204, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324037, Length=189, Percent_Identity=30.6878306878307, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6323204, Length=256, Percent_Identity=29.296875, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364189, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281364187, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281364185, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI281364183, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI62471759, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI17975520, Length=261, Percent_Identity=27.5862068965517, Blast_Score=102, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32609; Mature: 32609

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNGFFVIDKPAGVTSHDIVSKVRRAINQKKVGHTGTLDPFATGVLPVAVGEGTKAIQFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCHHHHHH
DESEKEYRAVMRLGIATDTQDLTGQVLAERDWSQLEAADLERLVPQFLGLQKQMPPMFSA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHH
IKQGGVPLYKLARKGIEVEREAREVEIHSLSFDWIRLPEACFTVTCSRGTYVRTLACDIG
HHHCCCHHHHHHHCCCCHHHHHHHEEEEEECCHHHCCCCHHEEEEECCCCEEEEHHHHHH
EALGCGAHLLELRRTRSGLFREKDAISIEALAAAANQQDLILPLDRALDHLRRLELSEAG
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC
GRKVQNGVVPLAADFTEATGEFQQGEQVRLYQGERFAAVAEYDMLKGLRLARVFN
CCCHHCCCCEEECCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLNGFFVIDKPAGVTSHDIVSKVRRAINQKKVGHTGTLDPFATGVLPVAVGEGTKAIQFL
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHCCEEEEECCCCHHHHHH
DESEKEYRAVMRLGIATDTQDLTGQVLAERDWSQLEAADLERLVPQFLGLQKQMPPMFSA
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCHHHCCCHHHHH
IKQGGVPLYKLARKGIEVEREAREVEIHSLSFDWIRLPEACFTVTCSRGTYVRTLACDIG
HHHCCCHHHHHHHCCCCHHHHHHHEEEEEECCHHHCCCCHHEEEEECCCCEEEEHHHHHH
EALGCGAHLLELRRTRSGLFREKDAISIEALAAAANQQDLILPLDRALDHLRRLELSEAG
HHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCC
GRKVQNGVVPLAADFTEATGEFQQGEQVRLYQGERFAAVAEYDMLKGLRLARVFN
CCCHHCCCCEEECCHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA