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The map label for this gene is tig

Identifier: 197117659

GI number: 197117659

Start: 1468074

End: 1469381

Strand: Direct

Name: tig

Synonym: Gbem_1271

Alternate gene names: 197117659

Gene position: 1468074-1469381 (Clockwise)

Preceding gene: 197117658

Following gene: 197117660

Centisome position: 31.81

GC content: 58.94

Gene sequence:

>1308_bases
ATGCAGATCAGTGTTGAATCGGTCAACAGCATCAAGAAGAAGTTGAACTTCGAAATCCCGGCAGACAAAGTTTCTGCTGA
AGTTGACAAAGCTTACGCCGAAATAAGAAAGCATGCCGCCATAAAGGGGTTCCGGAAGGGCAAGGTCCCGATGAGCCTGA
TCGAGAAGCACTACGGCGAGAAGATGGCCGAGGACGTGGTGAAAAACCTGGTCCAGGAAAGCTACTTCAGCGCGGTGAGC
GAGCAGGGGCTGAACCCGGTAGGGTACCCGGCTATCGAGAGCAACCCGCTGAAAAAGGGTGAGTCCTTCAAATATTCCGC
CACCGTCGAGGTCTTCCCTGAAGTCGAGGTGAAGGATTACACCGGCCTCGCAGTGGTCAAAGAGAAGCTTGAGATCGACG
ATTCCGTCGTAGCCGCGCGCCTGAAGGAGATGCAGGAGCGCATGTCCCAGCTTGGTCCGGCACCCGAAGGGCATGCAGCG
GCCATGGGCGACTTTGTTACCTTCGACTTCAAAGGCGCCATGGACGGCGTTTACTTCGAGGGGGGCTCCGCCGAGGACTT
CCAGCTCGAACTCGGCTCCGGGCGTTTCATCCCCGGCTTCGAGGAGCAGATGGTCGGCATGACCGTCGGCACCAACAGCA
CCATTAAGGTCAACTTCCCGGAAGGGTACGGCAACGCCGACTTGGCCGGCAAGCCTGCGGACTTCGAGGTCTCCATCAAG
GAGATCAAGGTGAAGGAGCTTCCGGAACTGAACGACGACTTCGCCAAGGAGTTCGGCGAGGAGTTCGAGACCCTGGACCT
GCTGAAGGCCAAGCTCGCCGAGATGAACGAGGCCCAGGAAGTCAGCCGCATCAACGCCGAACTGCGCGACCGCCTCATCA
AGGCCCTGATCGAGAAGAACGAGCTCGAGGTTCCCGAGGCGCTGGTGGACCGCCAGGCGCAGATGATGCTGGAGAGCACC
AAGCAGAGGCTCGCCTCCCAGAGGCTTTCCCTGGAGATGATGGGGATGACCGACGACAGCTACAAGGCGCAGTTCCGCGA
TAACGCCCGTGAGCAGGTAAAAGGTTCGCTGCTTCTGGACGCGGTCGCCGAGAAGGAAAAGATCGAGCCGACCGAAGAGG
AGTTCGAGGCGCAGCTCTCCGTGATCGCCGAGCAGACCAGGCAGGACCTGGAGAAGGTGACGCAGCTGTACAAGACCAAC
GAGCGGGCCAAGGACAACCTGATGGCCCAGATGAGGGAAGACAAGGCGGTGCAGTTCATCCTTGACCGCGCCAAGGTGAC
CGAAGTCCCCAAGGCGGAAATTAAATAG

Upstream 100 bases:

>100_bases
CCCCGACGACCCGTCACCAGAATTCCCGCCCAGACCCCATGCCATCAGGCATTACGGCAGAGAGCCTGCCCTATAATGTG
AACAAAAGAGAGGAATTTTC

Downstream 100 bases:

>100_bases
GAGTGTCCATGCTTGTACCGATAGTAGTGGAGCAGACCGGCCGGGGCGAGAGGTCGTACGACATCTACTCGCGCCTGCTG
AAGGACAGGATCATCTTCCT

Product: FKBP-type peptidylprolyl cis-trans isomerase (trigger factor)

Products: peptidylproline (omega=0)

Alternate protein names: TF

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MQISVESVNSIKKKLNFEIPADKVSAEVDKAYAEIRKHAAIKGFRKGKVPMSLIEKHYGEKMAEDVVKNLVQESYFSAVS
EQGLNPVGYPAIESNPLKKGESFKYSATVEVFPEVEVKDYTGLAVVKEKLEIDDSVVAARLKEMQERMSQLGPAPEGHAA
AMGDFVTFDFKGAMDGVYFEGGSAEDFQLELGSGRFIPGFEEQMVGMTVGTNSTIKVNFPEGYGNADLAGKPADFEVSIK
EIKVKELPELNDDFAKEFGEEFETLDLLKAKLAEMNEAQEVSRINAELRDRLIKALIEKNELEVPEALVDRQAQMMLEST
KQRLASQRLSLEMMGMTDDSYKAQFRDNAREQVKGSLLLDAVAEKEKIEPTEEEFEAQLSVIAEQTRQDLEKVTQLYKTN
ERAKDNLMAQMREDKAVQFILDRAKVTEVPKAEIK

Sequences:

>Translated_435_residues
MQISVESVNSIKKKLNFEIPADKVSAEVDKAYAEIRKHAAIKGFRKGKVPMSLIEKHYGEKMAEDVVKNLVQESYFSAVS
EQGLNPVGYPAIESNPLKKGESFKYSATVEVFPEVEVKDYTGLAVVKEKLEIDDSVVAARLKEMQERMSQLGPAPEGHAA
AMGDFVTFDFKGAMDGVYFEGGSAEDFQLELGSGRFIPGFEEQMVGMTVGTNSTIKVNFPEGYGNADLAGKPADFEVSIK
EIKVKELPELNDDFAKEFGEEFETLDLLKAKLAEMNEAQEVSRINAELRDRLIKALIEKNELEVPEALVDRQAQMMLEST
KQRLASQRLSLEMMGMTDDSYKAQFRDNAREQVKGSLLLDAVAEKEKIEPTEEEFEAQLSVIAEQTRQDLEKVTQLYKTN
ERAKDNLMAQMREDKAVQFILDRAKVTEVPKAEIK
>Mature_435_residues
MQISVESVNSIKKKLNFEIPADKVSAEVDKAYAEIRKHAAIKGFRKGKVPMSLIEKHYGEKMAEDVVKNLVQESYFSAVS
EQGLNPVGYPAIESNPLKKGESFKYSATVEVFPEVEVKDYTGLAVVKEKLEIDDSVVAARLKEMQERMSQLGPAPEGHAA
AMGDFVTFDFKGAMDGVYFEGGSAEDFQLELGSGRFIPGFEEQMVGMTVGTNSTIKVNFPEGYGNADLAGKPADFEVSIK
EIKVKELPELNDDFAKEFGEEFETLDLLKAKLAEMNEAQEVSRINAELRDRLIKALIEKNELEVPEALVDRQAQMMLEST
KQRLASQRLSLEMMGMTDDSYKAQFRDNAREQVKGSLLLDAVAEKEKIEPTEEEFEAQLSVIAEQTRQDLEKVTQLYKTN
ERAKDNLMAQMREDKAVQFILDRAKVTEVPKAEIK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain

Homologues:

Organism=Escherichia coli, GI1786640, Length=429, Percent_Identity=33.1002331002331, Blast_Score=232, Evalue=3e-62,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): TIG_GEOBB (B5EI26)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002138086.1
- GeneID:   6781255
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_1271
- HOGENOM:   HBG635105
- OMA:   RFIPGFE
- ProtClustDB:   CLSK828562
- HAMAP:   MF_00303
- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881
- Gene3D:   G3DSA:1.10.3120.10
- Gene3D:   G3DSA:3.30.70.1050
- PANTHER:   PTHR10516
- PIRSF:   PIRSF003095
- TIGRFAMs:   TIGR00115

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N; SSF109998 Trigger_fac_C_bac; SSF102735 Trigger_fac_ribosome-bd_bac

EC number: 5.2.1.8

Molecular weight: Translated: 48739; Mature: 48739

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQISVESVNSIKKKLNFEIPADKVSAEVDKAYAEIRKHAAIKGFRKGKVPMSLIEKHYGE
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KMAEDVVKNLVQESYFSAVSEQGLNPVGYPAIESNPLKKGESFKYSATVEVFPEVEVKDY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
TGLAVVKEKLEIDDSVVAARLKEMQERMSQLGPAPEGHAAAMGDFVTFDFKGAMDGVYFE
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEEEC
GGSAEDFQLELGSGRFIPGFEEQMVGMTVGTNSTIKVNFPEGYGNADLAGKPADFEVSIK
CCCCCCEEEEECCCCCCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHH
EIKVKELPELNDDFAKEFGEEFETLDLLKAKLAEMNEAQEVSRINAELRDRLIKALIEKN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELEVPEALVDRQAQMMLESTKQRLASQRLSLEMMGMTDDSYKAQFRDNAREQVKGSLLLD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCHHHHHHHHHHHH
AVAEKEKIEPTEEEFEAQLSVIAEQTRQDLEKVTQLYKTNERAKDNLMAQMREDKAVQFI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDRAKVTEVPKAEIK
HHHHHHCCCCCCCCC
>Mature Secondary Structure
MQISVESVNSIKKKLNFEIPADKVSAEVDKAYAEIRKHAAIKGFRKGKVPMSLIEKHYGE
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHH
KMAEDVVKNLVQESYFSAVSEQGLNPVGYPAIESNPLKKGESFKYSATVEVFPEVEVKDY
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
TGLAVVKEKLEIDDSVVAARLKEMQERMSQLGPAPEGHAAAMGDFVTFDFKGAMDGVYFE
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCEEEEEECCCCCCEEEC
GGSAEDFQLELGSGRFIPGFEEQMVGMTVGTNSTIKVNFPEGYGNADLAGKPADFEVSIK
CCCCCCEEEEECCCCCCCCHHHHHEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEEEHH
EIKVKELPELNDDFAKEFGEEFETLDLLKAKLAEMNEAQEVSRINAELRDRLIKALIEKN
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
ELEVPEALVDRQAQMMLESTKQRLASQRLSLEMMGMTDDSYKAQFRDNAREQVKGSLLLD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHCHHHHHHHHHHHH
AVAEKEKIEPTEEEFEAQLSVIAEQTRQDLEKVTQLYKTNERAKDNLMAQMREDKAVQFI
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
LDRAKVTEVPKAEIK
HHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (omega=180)

Specific reaction: peptidylproline (omega=180) = peptidylproline (omega=0)

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA