Click here to switch to the map view.

The map label for this gene is mscL-2 [H]

Identifier: 197117630

GI number: 197117630

Start: 1439054

End: 1439458

Strand: Direct

Name: mscL-2 [H]

Synonym: Gbem_1242

Alternate gene names: 197117630

Gene position: 1439054-1439458 (Clockwise)

Preceding gene: 197117629

Following gene: 308535225

Centisome position: 31.18

GC content: 45.43

Gene sequence:

>405_bases
ATGAGCATCGTTAAAGAGTTCAAGGAATTTGCCGTAAAAGGGAACGTCGTCGATATGGCGGTCGGTATTATAATTGGTGC
GGCATTTGCCAAAATAGTATCGTCGTTCGTCGGAGATGTTGTTATGCCGCCAATCGGAGTTCTTCTGGGAGGCGTTGATT
TTTCACAACTTTCCATCGTTGTTAAAGAAGCGGTTGACAAAAAGCCACCTGTGCTGATTAGTTACGGCAAGTTTATACAA
ACAGTCGTGGATTTTACTATCATTGCCATGGCAGTTTTCGCAGCAGTCAAGGCAATAAATACGCTCAAGAAGAAAGAAGC
TGCAGCGCCGGAGGTCCCCCCCGCACCGACACAACAAGAAATACTGCTTACTGAGATCAGGGACCTGCTTAAAAGCAACA
GATAA

Upstream 100 bases:

>100_bases
ACCGGTTTATCGGCTCCGGCGATGAATAGATGCCGCTAAACACTCATCTTGTCGAATTGCGAATGGATTTTCACCCATAC
TTCTTTCGGAGGTAGTGACT

Downstream 100 bases:

>100_bases
TACATCGGTTCAAGATGCTGCGAGGATTGGCTTACAAAGCATAATTTGGGGGTAGAAGATAGAGCCGCGAGAAGCAACAG
TAAGGCGTAGCTCCTTTTGC

Product: large-conductance mechanosensitive channel protein

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MSIVKEFKEFAVKGNVVDMAVGIIIGAAFAKIVSSFVGDVVMPPIGVLLGGVDFSQLSIVVKEAVDKKPPVLISYGKFIQ
TVVDFTIIAMAVFAAVKAINTLKKKEAAAPEVPPAPTQQEILLTEIRDLLKSNR

Sequences:

>Translated_134_residues
MSIVKEFKEFAVKGNVVDMAVGIIIGAAFAKIVSSFVGDVVMPPIGVLLGGVDFSQLSIVVKEAVDKKPPVLISYGKFIQ
TVVDFTIIAMAVFAAVKAINTLKKKEAAAPEVPPAPTQQEILLTEIRDLLKSNR
>Mature_133_residues
SIVKEFKEFAVKGNVVDMAVGIIIGAAFAKIVSSFVGDVVMPPIGVLLGGVDFSQLSIVVKEAVDKKPPVLISYGKFIQT
VVDFTIIAMAVFAAVKAINTLKKKEAAAPEVPPAPTQQEILLTEIRDLLKSNR

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=58.0882352941177, Blast_Score=165, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14311; Mature: 14180

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIVKEFKEFAVKGNVVDMAVGIIIGAAFAKIVSSFVGDVVMPPIGVLLGGVDFSQLSIV
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VKEAVDKKPPVLISYGKFIQTVVDFTIIAMAVFAAVKAINTLKKKEAAAPEVPPAPTQQE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ILLTEIRDLLKSNR
HHHHHHHHHHHCCC
>Mature Secondary Structure 
SIVKEFKEFAVKGNVVDMAVGIIIGAAFAKIVSSFVGDVVMPPIGVLLGGVDFSQLSIV
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VKEAVDKKPPVLISYGKFIQTVVDFTIIAMAVFAAVKAINTLKKKEAAAPEVPPAPTQQE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHH
ILLTEIRDLLKSNR
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA