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The map label for this gene is 197117551

Identifier: 197117551

GI number: 197117551

Start: 1352836

End: 1354170

Strand: Direct

Name: 197117551

Synonym: Gbem_1163

Alternate gene names: NA

Gene position: 1352836-1354170 (Clockwise)

Preceding gene: 197117550

Following gene: 197117553

Centisome position: 29.31

GC content: 64.64

Gene sequence:

>1335_bases
ATGAAACTTTGCATCATCTTCCCCCCGCTCCCCTTCGGCTGGACCCCGGTGGCCCCCCCGATTCTCGAGTACCTGGCCGC
TCTGACCAAGAAGGCGGATCCCACCATCGAGATCCAGTTGATCAGCGGGAGCGCGACGCCGGACGCCTTCGAGAAGCTCG
ACTGCGACCTGGCGGCGATCAGCATCCTGACTCCTACCGCCGTTCCCGGCTACCGCATCGCGGACTCCCTGAGAGCGCGC
GGCATCAAGGTGGTCTTCGGGGGGATGCACGCCTCCGCCATGCCCGAGGAGGCGAAGGAGCACGGCGACGCGGTGGTGAT
CGGGGAGGGGGAAAGCTGCTGGCCGCAGGTGCTCAAGGACTTCCGCGCCGGGACCCTGCAGCCCTTCTACCGCGGCGAAC
AGGTCGAGCTCTCCGACCTGCCGACCCCGCTTTACGGCTCCCTCGGGGGAGGGCACCAGTTCCGCATCGTGAACACCTCG
CGCGGCTGCCCCTTCAACTGCACCTTCTGCTCGGTGAAGCCGTTCTTCGGAGGGAAGATCCGCTTCCGCCCGATCGAGCA
CGTGGTGCGCGACGTGGCGGCCATACCCGAGAAGATGTACATAAACGGCGACGAGAACATCTGGTGGCCCGGCACGGAAA
ACATGCAGCGCGCCATAGACCTCTTCACCGCGCTCAGGGGAACCGGGAAAAAATGGATGGGGTTCGGCAGCCTCGGTCCC
GTTTTGGCCCCCCTGGGGTCGAAGATGCTGAACGCAGCGCGGGACTGCGGCATGCTCACCGTCTGGGTGGGGTGGGACGC
CATGACCGACGAGGGGCTCAAGGCCTACGCGGCCAACGGCAAGATCGGTGTGGACCGGGAGCGGGCGGTGCGCACGCTCA
GGGACCACGGCATCGACGTGTCGCTGTTCTACATGCTTGGCAGCAGGGACGATTCGCTAGACGACTTCAAACGCTCCGTC
GAACTCGCGGACCGGCTCGGGGTCTCCATGCACCCTTCGCTGGTGGTTCCCTACCCGGGGACCGAACTCGCCCGTCAGTA
CGAGCCGCACATCTTCAAGGAACTGGGGTGGGAGTACTACAACGGCTCCTACGCCGTTTTCGAGCACCCGGACCCGGCCA
TGACCCCTGAGGCCCGCGAGGAGCAGTTCTACGAAAGCTCGCTTGAGCTGTTGAGCCTGCCGCGCGTCCTGCGCCACATG
TTCAAGGTGCCGCTGCGCGGCTTCCCCTACGCCCACATGCTCTCCCTCATGAACCAGCTTCCGGTCAGAAAGGGGATGAA
GATCGCGTTCGAAAAGTGGAAGGCCAGCAAGGGTGAGGTGAATTTGAAGAGGTAG

Upstream 100 bases:

>100_bases
TACCCGCTGCCGGACGTGAAAAGCGTGCTCGATGCCATTTACAGCAACAGGAAAATCGACCTGTGACCCGGCGGGGAACG
CGCACTAAGGTAGCCGATAC

Downstream 100 bases:

>100_bases
GACGGCAGGGCCAGGGCCAGGGGCTGGGGGCTAGGGGCTAGGGGCTAGGGGCTAGTTTACCCCCTCCCGCCCTCCCCCCT
CCGGTGGGAGGGGGAAGGAT

Product: cobalamin-binding radical SAM domain-containing iron-sulfur cluster-binding oxidoreductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKLCIIFPPLPFGWTPVAPPILEYLAALTKKADPTIEIQLISGSATPDAFEKLDCDLAAISILTPTAVPGYRIADSLRAR
GIKVVFGGMHASAMPEEAKEHGDAVVIGEGESCWPQVLKDFRAGTLQPFYRGEQVELSDLPTPLYGSLGGGHQFRIVNTS
RGCPFNCTFCSVKPFFGGKIRFRPIEHVVRDVAAIPEKMYINGDENIWWPGTENMQRAIDLFTALRGTGKKWMGFGSLGP
VLAPLGSKMLNAARDCGMLTVWVGWDAMTDEGLKAYAANGKIGVDRERAVRTLRDHGIDVSLFYMLGSRDDSLDDFKRSV
ELADRLGVSMHPSLVVPYPGTELARQYEPHIFKELGWEYYNGSYAVFEHPDPAMTPEAREEQFYESSLELLSLPRVLRHM
FKVPLRGFPYAHMLSLMNQLPVRKGMKIAFEKWKASKGEVNLKR

Sequences:

>Translated_444_residues
MKLCIIFPPLPFGWTPVAPPILEYLAALTKKADPTIEIQLISGSATPDAFEKLDCDLAAISILTPTAVPGYRIADSLRAR
GIKVVFGGMHASAMPEEAKEHGDAVVIGEGESCWPQVLKDFRAGTLQPFYRGEQVELSDLPTPLYGSLGGGHQFRIVNTS
RGCPFNCTFCSVKPFFGGKIRFRPIEHVVRDVAAIPEKMYINGDENIWWPGTENMQRAIDLFTALRGTGKKWMGFGSLGP
VLAPLGSKMLNAARDCGMLTVWVGWDAMTDEGLKAYAANGKIGVDRERAVRTLRDHGIDVSLFYMLGSRDDSLDDFKRSV
ELADRLGVSMHPSLVVPYPGTELARQYEPHIFKELGWEYYNGSYAVFEHPDPAMTPEAREEQFYESSLELLSLPRVLRHM
FKVPLRGFPYAHMLSLMNQLPVRKGMKIAFEKWKASKGEVNLKR
>Mature_444_residues
MKLCIIFPPLPFGWTPVAPPILEYLAALTKKADPTIEIQLISGSATPDAFEKLDCDLAAISILTPTAVPGYRIADSLRAR
GIKVVFGGMHASAMPEEAKEHGDAVVIGEGESCWPQVLKDFRAGTLQPFYRGEQVELSDLPTPLYGSLGGGHQFRIVNTS
RGCPFNCTFCSVKPFFGGKIRFRPIEHVVRDVAAIPEKMYINGDENIWWPGTENMQRAIDLFTALRGTGKKWMGFGSLGP
VLAPLGSKMLNAARDCGMLTVWVGWDAMTDEGLKAYAANGKIGVDRERAVRTLRDHGIDVSLFYMLGSRDDSLDDFKRSV
ELADRLGVSMHPSLVVPYPGTELARQYEPHIFKELGWEYYNGSYAVFEHPDPAMTPEAREEQFYESSLELLSLPRVLRHM
FKVPLRGFPYAHMLSLMNQLPVRKGMKIAFEKWKASKGEVNLKR

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 49475; Mature: 49475

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLCIIFPPLPFGWTPVAPPILEYLAALTKKADPTIEIQLISGSATPDAFEKLDCDLAAI
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHCCCHHEE
SILTPTAVPGYRIADSLRARGIKVVFGGMHASAMPEEAKEHGDAVVIGEGESCWPQVLKD
EEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHH
FRAGTLQPFYRGEQVELSDLPTPLYGSLGGGHQFRIVNTSRGCPFNCTFCSVKPFFGGKI
HCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCEE
RFRPIEHVVRDVAAIPEKMYINGDENIWWPGTENMQRAIDLFTALRGTGKKWMGFGSLGP
EEEHHHHHHHHHHHCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCH
VLAPLGSKMLNAARDCGMLTVWVGWDAMTDEGLKAYAANGKIGVDRERAVRTLRDHGIDV
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCE
SLFYMLGSRDDSLDDFKRSVELADRLGVSMHPSLVVPYPGTELARQYEPHIFKELGWEYY
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHCCCHHHHHCCCEEE
NGSYAVFEHPDPAMTPEAREEQFYESSLELLSLPRVLRHMFKVPLRGFPYAHMLSLMNQL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
PVRKGMKIAFEKWKASKGEVNLKR
CHHCCHHHHHHHHCCCCCCEECCC
>Mature Secondary Structure
MKLCIIFPPLPFGWTPVAPPILEYLAALTKKADPTIEIQLISGSATPDAFEKLDCDLAAI
CEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHCCCHHEE
SILTPTAVPGYRIADSLRARGIKVVFGGMHASAMPEEAKEHGDAVVIGEGESCWPQVLKD
EEECCCCCCCHHHHHHHHHCCEEEEEECCCCCCCCHHHHHCCCEEEEECCCCHHHHHHHH
FRAGTLQPFYRGEQVELSDLPTPLYGSLGGGHQFRIVNTSRGCPFNCTFCSVKPFFGGKI
HCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCEEEEEEECCCCCCEE
RFRPIEHVVRDVAAIPEKMYINGDENIWWPGTENMQRAIDLFTALRGTGKKWMGFGSLGP
EEEHHHHHHHHHHHCCCEEEEECCCCEECCCCHHHHHHHHHHHHHHCCCCHHCCCCCCCH
VLAPLGSKMLNAARDCGMLTVWVGWDAMTDEGLKAYAANGKIGVDRERAVRTLRDHGIDV
HHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHCCCCE
SLFYMLGSRDDSLDDFKRSVELADRLGVSMHPSLVVPYPGTELARQYEPHIFKELGWEYY
EEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCCCHHHHHHCCCHHHHHCCCEEE
NGSYAVFEHPDPAMTPEAREEQFYESSLELLSLPRVLRHMFKVPLRGFPYAHMLSLMNQL
CCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHC
PVRKGMKIAFEKWKASKGEVNLKR
CHHCCHHHHHHHHCCCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]