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The map label for this gene is bchE [H]

Identifier: 197117553

GI number: 197117553

Start: 1356089

End: 1357609

Strand: Direct

Name: bchE [H]

Synonym: Gbem_1165

Alternate gene names: 197117553

Gene position: 1356089-1357609 (Clockwise)

Preceding gene: 197117551

Following gene: 197117554

Centisome position: 29.38

GC content: 62.66

Gene sequence:

>1521_bases
ATGACTATAAAAAGGGTCGCACTGATAACGCCGCCGTATCATTCCGGCGTCGTGGAGTCAGCGGGGACCTGGCTCAATGT
GGGGTTCGTATACGTTGCCGGGTCGCTGCGCGCAGCCGGCTACGAGGTGGACTACTACGACGCGATGAGCCTTTGGCACA
AGTGGCCGGAGATAGAGGCGCGCATCAGGGCCTTCAACCCGGACGTTGTGGCCACCACCTCGTTCACCGCCTCCATAGGG
CACGCGCTGGAGCTGACGGCGCTGGCCAAGGAGATCAACCCGGACGTGGTCACGGTGCACGGGAACGTGCACGCCACCTT
CTGCTACGACGAGATGCTCAAGGCCGATCACGACACGGTCGACTTCATCGTCCGGGGCGAGGGTGAAGTGACCCTGGTTA
AGCTTTTGGACTGCCTGAACCAAGGGGGCGACCCCGCCTCCGTCCCCGGACTTTCCTTCTGGCGCGACGGCGCCGTGGTC
ACGACGCCGAAGGCGGCGTCCATCCAGGACCTCGACGCGCTCCCCATGGCGTGGGACCTGGTGGAATGGCCCATCTACAC
CTACCGCGCCAAGAACAACGCCCGCCTGGCGATCGTCTCCTCGTCGCGCGGCTGCATGGAGAAATGCTCCTTCTGCTCGC
AGCAGCTCTTCTGGGAACGCTCCTGGCGCGCCCGAAGCGCCGAGAACTTCGTGGCCGAACTGGAGCTTCTGCGCGACAGC
TACGGCGTCGAGGTGGCGATGCTCTCCGACGAGATCCCGACCTTCGACCGCGAGCGCTGGGTGCGCATCCTGGACCTGAT
GATCGAAAGGAAGGTCGGGGTGAAGCTTTTGATGGAAACCCGGGTGGACGACATCCTGCGCGACGCGGACATCATGGACA
AGTACCGCGAGGCAGGGGTCGAGCACATCTACGTCGGGGTGGAAGCGGGGGACCAGGCGACGCTGGACCTCTTCAACAAG
AACACCAAGGTGGAGCAGTCCAAAGCCGCCATCGACATCATCAACAACGCCGACATCGTCTCCGAGACCTCGTTCGTCCT
GGGTATGCCGGACGACACGCCGGAGAGCATCGCGGCGACCATCGAGCTCGCCAAGCATTACAACCCGGACATGGCGTTTT
TCCTCGCCATCGCCCCCTGGCCCTACGCCGAGCTCTACCCCGAGCTGGAGCCGTACGTGGCGACCAAGGACTACCGCAAG
TACAACCTGGTGGAGCCGGTGATAAAGCCGAAGGCGATGACGGTGGAGGAGCTGGAAAGGGAGCTGGGCCGCGCGAGCCA
GAAGTTCTACATGCACAAGTTCCAGAAGCTGCACGACCTCTCCCCCTGGAAGCAGACCTTCATGGTCGCGGTTTTGGACA
TCCTCATCAACAACTCGTACCTGGCGGTGCAGATGAGGTCGATGATGAAGGAAGGAAAGCAGATGCCGCCCGAGGTGCAG
GCGCTTTTGAAAAGCATCAAGACGGTGCGCGGCAATGCCGGTGCCAGCGACCACCCGCACACCTTCGCGCCGATTCCGTA
A

Upstream 100 bases:

>100_bases
TGTATCTTTTCATTTCGATGGCCTCTGTTGCATAGAAAGCCAAACTTACTCAGAAATCGACTTCACTAACTACAGTTCTA
ATACCTGGAAGGGGTTGGAT

Downstream 100 bases:

>100_bases
GGGCTGGACAAGATTTTGGAGCAACATGGATTCGATCAAGAAGGCAGCCATCGTGCTGAACGGCTTCATCCACGATTTCG
CCACGGGCTACTGGCTCTCG

Product: radical SAM protein

Products: NA

Alternate protein names: Anaerobic Mg-protoporphyrin IX monomethyl ester oxidative cyclase [H]

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MTIKRVALITPPYHSGVVESAGTWLNVGFVYVAGSLRAAGYEVDYYDAMSLWHKWPEIEARIRAFNPDVVATTSFTASIG
HALELTALAKEINPDVVTVHGNVHATFCYDEMLKADHDTVDFIVRGEGEVTLVKLLDCLNQGGDPASVPGLSFWRDGAVV
TTPKAASIQDLDALPMAWDLVEWPIYTYRAKNNARLAIVSSSRGCMEKCSFCSQQLFWERSWRARSAENFVAELELLRDS
YGVEVAMLSDEIPTFDRERWVRILDLMIERKVGVKLLMETRVDDILRDADIMDKYREAGVEHIYVGVEAGDQATLDLFNK
NTKVEQSKAAIDIINNADIVSETSFVLGMPDDTPESIAATIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRK
YNLVEPVIKPKAMTVEELERELGRASQKFYMHKFQKLHDLSPWKQTFMVAVLDILINNSYLAVQMRSMMKEGKQMPPEVQ
ALLKSIKTVRGNAGASDHPHTFAPIP

Sequences:

>Translated_506_residues
MTIKRVALITPPYHSGVVESAGTWLNVGFVYVAGSLRAAGYEVDYYDAMSLWHKWPEIEARIRAFNPDVVATTSFTASIG
HALELTALAKEINPDVVTVHGNVHATFCYDEMLKADHDTVDFIVRGEGEVTLVKLLDCLNQGGDPASVPGLSFWRDGAVV
TTPKAASIQDLDALPMAWDLVEWPIYTYRAKNNARLAIVSSSRGCMEKCSFCSQQLFWERSWRARSAENFVAELELLRDS
YGVEVAMLSDEIPTFDRERWVRILDLMIERKVGVKLLMETRVDDILRDADIMDKYREAGVEHIYVGVEAGDQATLDLFNK
NTKVEQSKAAIDIINNADIVSETSFVLGMPDDTPESIAATIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRK
YNLVEPVIKPKAMTVEELERELGRASQKFYMHKFQKLHDLSPWKQTFMVAVLDILINNSYLAVQMRSMMKEGKQMPPEVQ
ALLKSIKTVRGNAGASDHPHTFAPIP
>Mature_505_residues
TIKRVALITPPYHSGVVESAGTWLNVGFVYVAGSLRAAGYEVDYYDAMSLWHKWPEIEARIRAFNPDVVATTSFTASIGH
ALELTALAKEINPDVVTVHGNVHATFCYDEMLKADHDTVDFIVRGEGEVTLVKLLDCLNQGGDPASVPGLSFWRDGAVVT
TPKAASIQDLDALPMAWDLVEWPIYTYRAKNNARLAIVSSSRGCMEKCSFCSQQLFWERSWRARSAENFVAELELLRDSY
GVEVAMLSDEIPTFDRERWVRILDLMIERKVGVKLLMETRVDDILRDADIMDKYREAGVEHIYVGVEAGDQATLDLFNKN
TKVEQSKAAIDIINNADIVSETSFVLGMPDDTPESIAATIELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRKY
NLVEPVIKPKAMTVEELERELGRASQKFYMHKFQKLHDLSPWKQTFMVAVLDILINNSYLAVQMRSMMKEGKQMPPEVQA
LLKSIKTVRGNAGASDHPHTFAPIP

Specific function: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis in anaerobic conditions. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-prot

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011772
- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 56968; Mature: 56837

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKRVALITPPYHSGVVESAGTWLNVGFVYVAGSLRAAGYEVDYYDAMSLWHKWPEIEA
CCCEEEEEECCCCCCCCHHCCCCEEEEEEEEEECCHHHCCEEECHHHHHHHHHCCCCHHH
RIRAFNPDVVATTSFTASIGHALELTALAKEINPDVVTVHGNVHATFCYDEMLKADHDTV
EEEECCCCEEEEECHHHHHCCHHHHHHHHHHCCCCEEEEECCEEEEEEHHHHHHCCCCCE
DFIVRGEGEVTLVKLLDCLNQGGDPASVPGLSFWRDGAVVTTPKAASIQDLDALPMAWDL
EEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHH
VEWPIYTYRAKNNARLAIVSSSRGCMEKCSFCSQQLFWERSWRARSAENFVAELELLRDS
HHCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
YGVEVAMLSDEIPTFDRERWVRILDLMIERKVGVKLLMETRVDDILRDADIMDKYREAGV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EHIYVGVEAGDQATLDLFNKNTKVEQSKAAIDIINNADIVSETSFVLGMPDDTPESIAAT
EEEEEEEECCCCCCHHHHCCCCCCCHHHHHEEEECCCCEEECCCEEEECCCCCHHHHHHH
IELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRKYNLVEPVIKPKAMTVEELER
HHHHHHCCCCEEEEEEECCCCHHHHCCCCCCEECCCCCCCCCCCCHHCCCCCCCHHHHHH
ELGRASQKFYMHKFQKLHDLSPWKQTFMVAVLDILINNSYLAVQMRSMMKEGKQMPPEVQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
ALLKSIKTVRGNAGASDHPHTFAPIP
HHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TIKRVALITPPYHSGVVESAGTWLNVGFVYVAGSLRAAGYEVDYYDAMSLWHKWPEIEA
CCEEEEEECCCCCCCCHHCCCCEEEEEEEEEECCHHHCCEEECHHHHHHHHHCCCCHHH
RIRAFNPDVVATTSFTASIGHALELTALAKEINPDVVTVHGNVHATFCYDEMLKADHDTV
EEEECCCCEEEEECHHHHHCCHHHHHHHHHHCCCCEEEEECCEEEEEEHHHHHHCCCCCE
DFIVRGEGEVTLVKLLDCLNQGGDPASVPGLSFWRDGAVVTTPKAASIQDLDALPMAWDL
EEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCHHHH
VEWPIYTYRAKNNARLAIVSSSRGCMEKCSFCSQQLFWERSWRARSAENFVAELELLRDS
HHCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHC
YGVEVAMLSDEIPTFDRERWVRILDLMIERKVGVKLLMETRVDDILRDADIMDKYREAGV
CCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EHIYVGVEAGDQATLDLFNKNTKVEQSKAAIDIINNADIVSETSFVLGMPDDTPESIAAT
EEEEEEEECCCCCCHHHHCCCCCCCHHHHHEEEECCCCEEECCCEEEECCCCCHHHHHHH
IELAKHYNPDMAFFLAIAPWPYAELYPELEPYVATKDYRKYNLVEPVIKPKAMTVEELER
HHHHHHCCCCEEEEEEECCCCHHHHCCCCCCEECCCCCCCCCCCCHHCCCCCCCHHHHHH
ELGRASQKFYMHKFQKLHDLSPWKQTFMVAVLDILINNSYLAVQMRSMMKEGKQMPPEVQ
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
ALLKSIKTVRGNAGASDHPHTFAPIP
HHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8385667; 2203738; 10841582 [H]