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The map label for this gene is lacA [C]

Identifier: 197117455

GI number: 197117455

Start: 1238308

End: 1238835

Strand: Direct

Name: lacA [C]

Synonym: Gbem_1066

Alternate gene names: 197117455

Gene position: 1238308-1238835 (Clockwise)

Preceding gene: 197117454

Following gene: 197117456

Centisome position: 26.83

GC content: 53.41

Gene sequence:

>528_bases
ATGAACGAGAATCTTGTTGCTGTTGCCGACGACGTGAAGCTCGGGGCGAACGTAAAACTAGGGAAATTCATCAACCTGTA
CGGGTGCAGTATTGGTGACCACACCAAGATCGGCCCCTTCGTAGAAATCCAGAAGAATGCGGAGATCGGGAAGAACTGCA
AGATCTCCAGTCACAGCTTCATCTGTGATGGTGTGGTGATCGAAGACAATGTCTTCGTCGGTCACAACGTGACCTTCATC
AACGACCTGTATCCGAGGGCCACCACCTCCTCCGGCGAACTGCAGGTTGAGGCGGACTGGGCCTGCATCAGGACCACCAT
AAAGAGAAATGCCTCCATAGGCTCCAGTTCCACCATCCTTTGCGGTGTCACTGTCGGCGAACACGCCATTGTCGGTGCCG
GAAGCGTCGTCACCAAGGACGTTCAACCCTACAGCATCGTGGCTGGCAATCCCGCAAGACTGCTGCGTACCATCCCCAAG
GAGACTGATGATGAAAGTGCCATTTCTCAACCTCAAAGCACAATATGA

Upstream 100 bases:

>100_bases
CGGGACTAAGGGTGGTCAGGATACTGGAGGCGATCAAGAAGTCTCTGGCCAAGGGAGGAGCACTAGTCCGCCTGAACTGA
GAGGGGAAAAGGAGAAAGGA

Downstream 100 bases:

>100_bases
CTCCATAGCACATGAGGTGGAACAGTCGATCCGGGAGGTGCTCTCCACCTGTGCCTTTTCCGGCGGACCCTACGTGCAGA
AGTTCGAGGAGGAGTTCGCT

Product: acyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: NA

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MNENLVAVADDVKLGANVKLGKFINLYGCSIGDHTKIGPFVEIQKNAEIGKNCKISSHSFICDGVVIEDNVFVGHNVTFI
NDLYPRATTSSGELQVEADWACIRTTIKRNASIGSSSTILCGVTVGEHAIVGAGSVVTKDVQPYSIVAGNPARLLRTIPK
ETDDESAISQPQSTI

Sequences:

>Translated_175_residues
MNENLVAVADDVKLGANVKLGKFINLYGCSIGDHTKIGPFVEIQKNAEIGKNCKISSHSFICDGVVIEDNVFVGHNVTFI
NDLYPRATTSSGELQVEADWACIRTTIKRNASIGSSSTILCGVTVGEHAIVGAGSVVTKDVQPYSIVAGNPARLLRTIPK
ETDDESAISQPQSTI
>Mature_175_residues
MNENLVAVADDVKLGANVKLGKFINLYGCSIGDHTKIGPFVEIQKNAEIGKNCKISSHSFICDGVVIEDNVFVGHNVTFI
NDLYPRATTSSGELQVEADWACIRTTIKRNASIGSSSTILCGVTVGEHAIVGAGSVVTKDVQPYSIVAGNPARLLRTIPK
ETDDESAISQPQSTI

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Escherichia coli, GI1786537, Length=134, Percent_Identity=33.5820895522388, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001451
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00132 Hexapep [H]

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 18618; Mature: 18618

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS00101 HEXAPEP_TRANSFERASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNENLVAVADDVKLGANVKLGKFINLYGCSIGDHTKIGPFVEIQKNAEIGKNCKISSHSF
CCCCEEEEECCEEECCCEEECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCE
ICDGVVIEDNVFVGHNVTFINDLYPRATTSSGELQVEADWACIRTTIKRNASIGSSSTIL
EECCEEEECCEEECCCEEEEECCCCCCCCCCCCEEEEECHHEEHHHHHCCCCCCCCCEEE
CGVTVGEHAIVGAGSVVTKDVQPYSIVAGNPARLLRTIPKETDDESAISQPQSTI
EEEEECCEEEEECCCEEECCCCCEEEEECCHHHHHHHCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MNENLVAVADDVKLGANVKLGKFINLYGCSIGDHTKIGPFVEIQKNAEIGKNCKISSHSF
CCCCEEEEECCEEECCCEEECCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCEECCCCE
ICDGVVIEDNVFVGHNVTFINDLYPRATTSSGELQVEADWACIRTTIKRNASIGSSSTIL
EECCEEEECCEEECCCEEEEECCCCCCCCCCCCEEEEECHHEEHHHHHCCCCCCCCCEEE
CGVTVGEHAIVGAGSVVTKDVQPYSIVAGNPARLLRTIPKETDDESAISQPQSTI
EEEEECCEEEEECCCEEECCCCCEEEEECCHHHHHHHCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]