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The map label for this gene is cpaF-1 [C]

Identifier: 197117444

GI number: 197117444

Start: 1227314

End: 1228627

Strand: Reverse

Name: cpaF-1 [C]

Synonym: Gbem_1055

Alternate gene names: 197117444

Gene position: 1228627-1227314 (Counterclockwise)

Preceding gene: 197117445

Following gene: 197117443

Centisome position: 26.62

GC content: 63.55

Gene sequence:

>1314_bases
ATGCTGCTCGATGGTATCAAGCCTCAGGCGGTGCAGGGTCCGGCAGCGACGGAATCGGAACTCTTCAAGGAGATGAAGAC
CCGGATCCACCGCCGCCTGATCGACAGCATCGACCTCGCCAAGATGGACCTGATGCGCAACAACGAGATGGTGAGCCAGA
TCCGCGGCGTAATAGAGAACCTCATCGTCGGCGAGGGGGTCCCGCTGAACCAGATGGAGAGGGACCGGCTGGTGCTGGAG
ATCCAGCACGAGACCTTCGGCCTCGGGCCGCTCGAGCCTCTTCTGGCCGACGACCAGATCTCCGACATCCTGGTGAACGA
CAGCTCCAACGTCTACATCGAAAAGGGGGGGAAGCTGCAAAAGACCGAGGTCTGCTTCCGGGACAACGCGCACCTGATGC
AAATAATCGAGCGCATCGTCTCCAAGATCGGGAGAAGGATCGACGAGTCCTCCCCCTACGTCGACGCGAGGCTTCCCGAC
GGCTCCCGCGTGAACGCCATCATCCCCCCCCTGGCGCTGAACGGCCCTGTGCTCTCCATCAGGCGCTTCGGGCTGGAGCC
GCTGGTCATGAGGGACCTGATCAAGCTGCAGGCCCTGGACCAGAGGATGGCGGAGCTTTTGGAGGCGGCGGTGCAGACGA
GGCTCAACATCCTGATCTCCGGGGGTACCGGCACCGGCAAGACCACCCTCCTGAACGTCCTCTCCGCCTTCATCCCCGGG
GGGGAGCGGCTCATCACCATCGAGGATTCGGCCGAGCTGAACCTGAAGCAGGAGCACGTGGTGCGGCTGGAAACCAGGCC
CCCCAACCTTGAGGGGCGCGGCGAGGTGACCTCGCGTGACCTGCTGAAAAACGCGCTGAGGATGCGGCCGGATCGAATCA
TCATCGGCGAGGTGCGCGGCGGCGAGGCGCTCGACCTGCTGCAGGCGATGAACACCGGCCACGACGGCTCGCTCTCCACG
GTCCACGCCAACACCACCCGCGACGCCCTCTCACGCATGGAAACCATGGTCCTTATGGCCGGGCTGGACCTTCCGGAGAG
GGCCATCAAGGAACAGATGGCCTCGGCCCTAGACCTGGTGGTGCAACTGGTCCGCTTCTCCGACGGGACCCGGAAGGTGG
TGAAACTCTCCGAGATCACCGGGATGGAGGGGAACACCATCGTCATGCACGACATCATCGTGTTCCTGCAAAAGGGGATC
GACCGGCAAGGGAACGTGATCGGCGAATTCCGCGCCACCGGGGTGAGGCCGCGCTTTGCCGAGCGCTTCAAGCTCTACGG
GTTCGAGCTCCCCGCCATCATGTTCGAGAACTAG

Upstream 100 bases:

>100_bases
AGCAAGGCAATCCAGGGGCTGGCGGAGCGGGTGAAGCGCTGACCGGGAGACGCCCGGCGCCGCACACTTTTTGCGGCGCT
CCGGGCAAGGGGGAGAGATC

Downstream 100 bases:

>100_bases
GAGCCCCGCATGCTTTATCCCATCGTGGCGCTTATATTCCTGGCGGCCTTCGGCGTCTTCCTGGCGGCCTTTCTCTACCT
CGCGCAACGGCAGACGTCCG

Product: Flp pilus assembly ATPase CpaF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MLLDGIKPQAVQGPAATESELFKEMKTRIHRRLIDSIDLAKMDLMRNNEMVSQIRGVIENLIVGEGVPLNQMERDRLVLE
IQHETFGLGPLEPLLADDQISDILVNDSSNVYIEKGGKLQKTEVCFRDNAHLMQIIERIVSKIGRRIDESSPYVDARLPD
GSRVNAIIPPLALNGPVLSIRRFGLEPLVMRDLIKLQALDQRMAELLEAAVQTRLNILISGGTGTGKTTLLNVLSAFIPG
GERLITIEDSAELNLKQEHVVRLETRPPNLEGRGEVTSRDLLKNALRMRPDRIIIGEVRGGEALDLLQAMNTGHDGSLST
VHANTTRDALSRMETMVLMAGLDLPERAIKEQMASALDLVVQLVRFSDGTRKVVKLSEITGMEGNTIVMHDIIVFLQKGI
DRQGNVIGEFRATGVRPRFAERFKLYGFELPAIMFEN

Sequences:

>Translated_437_residues
MLLDGIKPQAVQGPAATESELFKEMKTRIHRRLIDSIDLAKMDLMRNNEMVSQIRGVIENLIVGEGVPLNQMERDRLVLE
IQHETFGLGPLEPLLADDQISDILVNDSSNVYIEKGGKLQKTEVCFRDNAHLMQIIERIVSKIGRRIDESSPYVDARLPD
GSRVNAIIPPLALNGPVLSIRRFGLEPLVMRDLIKLQALDQRMAELLEAAVQTRLNILISGGTGTGKTTLLNVLSAFIPG
GERLITIEDSAELNLKQEHVVRLETRPPNLEGRGEVTSRDLLKNALRMRPDRIIIGEVRGGEALDLLQAMNTGHDGSLST
VHANTTRDALSRMETMVLMAGLDLPERAIKEQMASALDLVVQLVRFSDGTRKVVKLSEITGMEGNTIVMHDIIVFLQKGI
DRQGNVIGEFRATGVRPRFAERFKLYGFELPAIMFEN
>Mature_437_residues
MLLDGIKPQAVQGPAATESELFKEMKTRIHRRLIDSIDLAKMDLMRNNEMVSQIRGVIENLIVGEGVPLNQMERDRLVLE
IQHETFGLGPLEPLLADDQISDILVNDSSNVYIEKGGKLQKTEVCFRDNAHLMQIIERIVSKIGRRIDESSPYVDARLPD
GSRVNAIIPPLALNGPVLSIRRFGLEPLVMRDLIKLQALDQRMAELLEAAVQTRLNILISGGTGTGKTTLLNVLSAFIPG
GERLITIEDSAELNLKQEHVVRLETRPPNLEGRGEVTSRDLLKNALRMRPDRIIIGEVRGGEALDLLQAMNTGHDGSLST
VHANTTRDALSRMETMVLMAGLDLPERAIKEQMASALDLVVQLVRFSDGTRKVVKLSEITGMEGNTIVMHDIIVFLQKGI
DRQGNVIGEFRATGVRPRFAERFKLYGFELPAIMFEN

Specific function: Unknown

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1786296, Length=200, Percent_Identity=30, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789723, Length=204, Percent_Identity=31.8627450980392, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 48614; Mature: 48614

Theoretical pI: Translated: 5.77; Mature: 5.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLDGIKPQAVQGPAATESELFKEMKTRIHRRLIDSIDLAKMDLMRNNEMVSQIRGVIEN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LIVGEGVPLNQMERDRLVLEIQHETFGLGPLEPLLADDQISDILVNDSSNVYIEKGGKLQ
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHEEEECCCCEEEECCCCEE
KTEVCFRDNAHLMQIIERIVSKIGRRIDESSPYVDARLPDGSRVNAIIPPLALNGPVLSI
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHH
RRFGLEPLVMRDLIKLQALDQRMAELLEAAVQTRLNILISGGTGTGKTTLLNVLSAFIPG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCC
GERLITIEDSAELNLKQEHVVRLETRPPNLEGRGEVTSRDLLKNALRMRPDRIIIGEVRG
CCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
GEALDLLQAMNTGHDGSLSTVHANTTRDALSRMETMVLMAGLDLPERAIKEQMASALDLV
CHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VQLVRFSDGTRKVVKLSEITGMEGNTIVMHDIIVFLQKGIDRQGNVIGEFRATGVRPRFA
HHHHHCCCCCHHHEEEHHHCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEHHCCCCCHHHH
ERFKLYGFELPAIMFEN
HHHHHCCEECCEEEECC
>Mature Secondary Structure
MLLDGIKPQAVQGPAATESELFKEMKTRIHRRLIDSIDLAKMDLMRNNEMVSQIRGVIEN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
LIVGEGVPLNQMERDRLVLEIQHETFGLGPLEPLLADDQISDILVNDSSNVYIEKGGKLQ
HHHCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHCCCCHHHEEEECCCCEEEECCCCEE
KTEVCFRDNAHLMQIIERIVSKIGRRIDESSPYVDARLPDGSRVNAIIPPLALNGPVLSI
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEECCCCCCCCCCHHH
RRFGLEPLVMRDLIKLQALDQRMAELLEAAVQTRLNILISGGTGTGKTTLLNVLSAFIPG
HHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHHHHHHHHHCCC
GERLITIEDSAELNLKQEHVVRLETRPPNLEGRGEVTSRDLLKNALRMRPDRIIIGEVRG
CCEEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEECCC
GEALDLLQAMNTGHDGSLSTVHANTTRDALSRMETMVLMAGLDLPERAIKEQMASALDLV
CHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
VQLVRFSDGTRKVVKLSEITGMEGNTIVMHDIIVFLQKGIDRQGNVIGEFRATGVRPRFA
HHHHHCCCCCHHHEEEHHHCCCCCCEEHHHHHHHHHHHCCCCCCCEEEEHHCCCCCHHHH
ERFKLYGFELPAIMFEN
HHHHHCCEECCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]