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The map label for this gene is nifA [H]

Identifier: 197116704

GI number: 197116704

Start: 376924

End: 378549

Strand: Direct

Name: nifA [H]

Synonym: Gbem_0304

Alternate gene names: 197116704

Gene position: 376924-378549 (Clockwise)

Preceding gene: 197116701

Following gene: 197116705

Centisome position: 8.17

GC content: 58.43

Gene sequence:

>1626_bases
ATGAGTGTCATAGTCTACTTAGCCGCTACCAAAACCTCATTGACATTCAATGTACATAGCCAGTACATTCACTGCATGAA
ATCCTCAAAAGCTACAGACAAAGAGTGGACCTTTTTAGAGACAGTGGCGCGCGCCGCATTTGCCAATCCGTTCGGCGAAA
CTAGAGACGACCTCGACATAGCCATAAGCGGAGCCGCACCTGATTCGTCGGCCGACGAGATCCTGCATGCGGTAGTGACA
CGTATCGAGGTCAAATTGCAGAGCCTGCGCGACAGAGGAGTAGCCGACCTGCGTAACCTGGACGGACACCGGCGCGAGGT
CATGCGCAGGGTTTGCCTCTTCCATGTCTTTCACCGCTACGTGGACCATTTCGACGAGCTGATAAAGACCCAACTGAAGG
AGGGGGACAAGCATTGCCGCGTCCCCTTCGCCCGGCACGTCCTTGGGGAACTGACCGCTTTCGGCATAGGCGATCAGGAT
GCGGTGCGCATGTTCGCCGTATTCTACCAGCTGCGCCGTGCCTTTTACTTCATCAAGAACGGGCTGGTGGGCACTTCCCC
CTCCATGAAGACCTTGCGCCGGCATCTCTGGGACTGCGTCTTCACCCACGACATAAGGTGGTTCGAGGCGGGCCTATGGG
ACAAGATGGAGGAATTCTCCATACTGCTTTTAGGGGAAACCGGCACCGGCAAGGGGGCCGCGGCCGCCGCCATCGGGCGC
TCCGGGTTCATTCCCTTCGACGTCGCCAGAAACGCCTTCACCGAGAGCTTTACCCGCAACTTCGTCTCCATAAACCTGTC
GCAGTACTCCGAAGGTGTGCTGGAATCCGAACTCTTCGGGCACAAGAAAGGCGCCTTCACCGGCGCCGTCGACAACCACG
AAGGGCTCTTCAGCCGCTGCGCCCCGCATGGCGTCATCTTCCTGGACGAGATAGGCGACGTGAGCATTCCGGTGCAGATA
AAACTGCTCCAGGTGCTGCAGGAGCGAATCTTCTTCCCTGTGGGGAGCCACGAGGCCAGGCGCTTCAGCGGGCGCATCGT
CGCCGCTACCAACCGTTCGCTAGACGAGATGCTCGAAAGCGGAAAGTTCCGCGACGACCTCTACTATCGCCTCTGTTCCG
ACGTCATCACCCTGCCGCCGCTCAGAGTGAGGCTGAAAGAGGACCCGCAGGAGCTGGACAACCTTCTGGGCGTTCTTCTT
TCGCGTCTTGCCGGGGAGATGCCGGGGCGGGAAAAGCATCTGGTGAAAAGGATACTGAAGCGCGACCTGGGGCGTCATTA
CGCGTGGCCCGGCAACGTGCGGGAGCTGGAACAGGCAATAAAGAGGATCATCCTCACCGGGCGCTATCAAGGAATGAAAA
GAGAGGAACCGAAGCAGGATGCCGCAGCCCGGCTTGCGCAGGAGATAGTGGAAGGTGCAATGAACGCCGAGGAATTGCTC
TCCGCCTACTGCTCGTCCCTTTATGCCAGCTTCGGCACCTATGAAGAGGTAGCCCGCAGGACCCGCCTGGACCGGCGCAC
CGTCAAGAAGTACGTGGAGATGGGAAAAGTCTCCCCCTCCCTGGACGGGAGGGGGACTTTATTCGGCAACTGCAGTATCC
AAACCGGACGATGTTTGGAAAGTTGA

Upstream 100 bases:

>100_bases
GGACAGGGGGGATTTCACCTTACTTGCACTCTCACTATTACAGGGACTATGCCAATAGATGCTTTTATTGCTCTGACACT
GTAACCCCTTGTTTTATGCA

Downstream 100 bases:

>100_bases
GACTGAATCCCATTGACAAAGAGCGCAGCATAGGCAATAACAAGCCTTTATTTGAATTGATTAAGCAGTTGCCGTCTTGC
TATCCGAGGCATCTTTCTTT

Product: sigma-54-dependent transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MSVIVYLAATKTSLTFNVHSQYIHCMKSSKATDKEWTFLETVARAAFANPFGETRDDLDIAISGAAPDSSADEILHAVVT
RIEVKLQSLRDRGVADLRNLDGHRREVMRRVCLFHVFHRYVDHFDELIKTQLKEGDKHCRVPFARHVLGELTAFGIGDQD
AVRMFAVFYQLRRAFYFIKNGLVGTSPSMKTLRRHLWDCVFTHDIRWFEAGLWDKMEEFSILLLGETGTGKGAAAAAIGR
SGFIPFDVARNAFTESFTRNFVSINLSQYSEGVLESELFGHKKGAFTGAVDNHEGLFSRCAPHGVIFLDEIGDVSIPVQI
KLLQVLQERIFFPVGSHEARRFSGRIVAATNRSLDEMLESGKFRDDLYYRLCSDVITLPPLRVRLKEDPQELDNLLGVLL
SRLAGEMPGREKHLVKRILKRDLGRHYAWPGNVRELEQAIKRIILTGRYQGMKREEPKQDAAARLAQEIVEGAMNAEELL
SAYCSSLYASFGTYEEVARRTRLDRRTVKKYVEMGKVSPSLDGRGTLFGNCSIQTGRCLES

Sequences:

>Translated_541_residues
MSVIVYLAATKTSLTFNVHSQYIHCMKSSKATDKEWTFLETVARAAFANPFGETRDDLDIAISGAAPDSSADEILHAVVT
RIEVKLQSLRDRGVADLRNLDGHRREVMRRVCLFHVFHRYVDHFDELIKTQLKEGDKHCRVPFARHVLGELTAFGIGDQD
AVRMFAVFYQLRRAFYFIKNGLVGTSPSMKTLRRHLWDCVFTHDIRWFEAGLWDKMEEFSILLLGETGTGKGAAAAAIGR
SGFIPFDVARNAFTESFTRNFVSINLSQYSEGVLESELFGHKKGAFTGAVDNHEGLFSRCAPHGVIFLDEIGDVSIPVQI
KLLQVLQERIFFPVGSHEARRFSGRIVAATNRSLDEMLESGKFRDDLYYRLCSDVITLPPLRVRLKEDPQELDNLLGVLL
SRLAGEMPGREKHLVKRILKRDLGRHYAWPGNVRELEQAIKRIILTGRYQGMKREEPKQDAAARLAQEIVEGAMNAEELL
SAYCSSLYASFGTYEEVARRTRLDRRTVKKYVEMGKVSPSLDGRGTLFGNCSIQTGRCLES
>Mature_540_residues
SVIVYLAATKTSLTFNVHSQYIHCMKSSKATDKEWTFLETVARAAFANPFGETRDDLDIAISGAAPDSSADEILHAVVTR
IEVKLQSLRDRGVADLRNLDGHRREVMRRVCLFHVFHRYVDHFDELIKTQLKEGDKHCRVPFARHVLGELTAFGIGDQDA
VRMFAVFYQLRRAFYFIKNGLVGTSPSMKTLRRHLWDCVFTHDIRWFEAGLWDKMEEFSILLLGETGTGKGAAAAAIGRS
GFIPFDVARNAFTESFTRNFVSINLSQYSEGVLESELFGHKKGAFTGAVDNHEGLFSRCAPHGVIFLDEIGDVSIPVQIK
LLQVLQERIFFPVGSHEARRFSGRIVAATNRSLDEMLESGKFRDDLYYRLCSDVITLPPLRVRLKEDPQELDNLLGVLLS
RLAGEMPGREKHLVKRILKRDLGRHYAWPGNVRELEQAIKRIILTGRYQGMKREEPKQDAAARLAQEIVEGAMNAEELLS
AYCSSLYASFGTYEEVARRTRLDRRTVKKYVEMGKVSPSLDGRGTLFGNCSIQTGRCLES

Specific function: Required for activation of most nif operons, which are directly involved in nitrogen fixation [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=339, Percent_Identity=35.9882005899705, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1788905, Length=242, Percent_Identity=40.0826446280992, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87082117, Length=229, Percent_Identity=39.7379912663755, Blast_Score=146, Evalue=4e-36,
Organism=Escherichia coli, GI1788550, Length=231, Percent_Identity=39.8268398268398, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082152, Length=273, Percent_Identity=36.2637362637363, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1786524, Length=270, Percent_Identity=33.7037037037037, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789087, Length=230, Percent_Identity=41.7391304347826, Blast_Score=142, Evalue=6e-35,
Organism=Escherichia coli, GI1789233, Length=226, Percent_Identity=38.0530973451327, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI1790299, Length=223, Percent_Identity=38.1165919282511, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=34.8017621145374, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1787583, Length=288, Percent_Identity=31.25, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=35.4260089686099, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081858, Length=230, Percent_Identity=26.9565217391304, Blast_Score=82, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR010113
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 61166; Mature: 61035

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVIVYLAATKTSLTFNVHSQYIHCMKSSKATDKEWTFLETVARAAFANPFGETRDDLDI
CCEEEEEEECCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEE
AISGAAPDSSADEILHAVVTRIEVKLQSLRDRGVADLRNLDGHRREVMRRVCLFHVFHRY
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
VDHFDELIKTQLKEGDKHCRVPFARHVLGELTAFGIGDQDAVRMFAVFYQLRRAFYFIKN
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHC
GLVGTSPSMKTLRRHLWDCVFTHDIRWFEAGLWDKMEEFSILLLGETGTGKGAAAAAIGR
CCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCEEEEEECCCCCCCHHHHHHCC
SGFIPFDVARNAFTESFTRNFVSINLSQYSEGVLESELFGHKKGAFTGAVDNHEGLFSRC
CCCCCHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHC
APHGVIFLDEIGDVSIPVQIKLLQVLQERIFFPVGSHEARRFSGRIVAATNRSLDEMLES
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHCCEEEEECCCCHHHHHHC
GKFRDDLYYRLCSDVITLPPLRVRLKEDPQELDNLLGVLLSRLAGEMPGREKHLVKRILK
CCCHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RDLGRHYAWPGNVRELEQAIKRIILTGRYQGMKREEPKQDAAARLAQEIVEGAMNAEELL
HHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
SAYCSSLYASFGTYEEVARRTRLDRRTVKKYVEMGKVSPSLDGRGTLFGNCSIQTGRCLE
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCC
S
C
>Mature Secondary Structure 
SVIVYLAATKTSLTFNVHSQYIHCMKSSKATDKEWTFLETVARAAFANPFGETRDDLDI
CEEEEEEECCEEEEEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCEE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C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2840552; 2450865 [H]