Click here to switch to the map view.

The map label for this gene is htrB [H]

Identifier: 197116624

GI number: 197116624

Start: 264179

End: 265174

Strand: Reverse

Name: htrB [H]

Synonym: Gbem_0224

Alternate gene names: 197116624

Gene position: 265174-264179 (Counterclockwise)

Preceding gene: 197116625

Following gene: 197116622

Centisome position: 5.75

GC content: 61.45

Gene sequence:

>996_bases
GTGAGTAATCAGGCACCACGCGTACCTAATCGCGCGGCAGTTGCACCGGTTTTCGCTTGGTGGGCCCTCCAGTCAGCTAT
TTTTTATGGACTAACCCTCCTCGCCAGCAGCCTGCCGATATCCTTGAGCCGACGATTCGGCGCTTGGGGAGGGGCGTTCA
TCCCCCGGATCCTCCCGCGACGTCTCGATGTGGCTGTGGATAACATCAGCCGCGCGCTTCCTTTCATGCAGGCCCACCCC
CTCTGGAACCCTCAAAATCCACCCGTTGAAGAACTGGCGCACCAAAACTTCAGGAACCTTGGTCGCAATCTGGTCGAGAT
CAGCCGGCTCTACCACGGCCGTGAAGAAGGTGTGCTGGACGTCATCGAACTGCGCGGCCGGGAAAATCTGGACCGCGCCA
AGGGCAAAGGAAAAGGGGTGGTTTGCTTCAGCGGCCATTGCGGGAATTGGGAAGTGATGGCGCTGGCGCTGGGGAGCATT
TTCGGCGACGGCGTGGTAGTGGCCCGGCGCCAGAAGAATCCTTTTCTGGAGAAGATGATCACCCGGATGCGCATGCGCTA
TAACAGCCGCGTCCTGGACAAGCAGGGGGCACTGAGGGGGATACTGAAAGCCCTCAAGAAAGGGGAACTGGTAGGGATCC
TGGCTGACCAGGCTGTCCTCCCCGAAGAAGGAGTGCTCATCGAGGTGATGGGGCGGCAGGCTTGGGCCTCCAAGGCTCCC
GTGATCATTGCCAGGGCGAGCGGAGCCGCGGTGGTGCCCGTCTTCATCCATCGTGAGGAGGGGCGGCAGGTAATAACCTT
CCATCCGGAGTACCGCTTCAGCAGCGACGAGAGCGAGGCGGGAATCACCAGCGACATCCAGGCGTTGTCCCGGTACGTGG
AGGATTTCGTAGTGGCGCACCCGACCCAGTGGTACTGGGTGCATCGACGTTGGAAGCGCGCCTTTGCGGCAGGCGCGGCG
CCGTTGGACGAGGTCGCACTGTTGACAGCCGGCTGA

Upstream 100 bases:

>100_bases
GGTCTATGGTGCAGCCGGAATCGCAGTAATTAAAGCACCCGCCTCTTGGCAAGCGCCTGGCAAGCGTCGGCTTCCAGGCT
CCCTTCCAAGGAGGAGCGTT

Downstream 100 bases:

>100_bases
TCGCCGGCCACAGTCGTGTACCGCGCTAGCGGCAAAAGAGTCTTGCCGTACGGCAGGTAAAGAAATTGAAAGCCCTCGGC
AATTGCCGGGGGCTTTCAAT

Product: lipid A biosynthesis lauroyl/myristoyl acyltransferase

Products: NA

Alternate protein names: Heat shock protein B [H]

Number of amino acids: Translated: 331; Mature: 330

Protein sequence:

>331_residues
MSNQAPRVPNRAAVAPVFAWWALQSAIFYGLTLLASSLPISLSRRFGAWGGAFIPRILPRRLDVAVDNISRALPFMQAHP
LWNPQNPPVEELAHQNFRNLGRNLVEISRLYHGREEGVLDVIELRGRENLDRAKGKGKGVVCFSGHCGNWEVMALALGSI
FGDGVVVARRQKNPFLEKMITRMRMRYNSRVLDKQGALRGILKALKKGELVGILADQAVLPEEGVLIEVMGRQAWASKAP
VIIARASGAAVVPVFIHREEGRQVITFHPEYRFSSDESEAGITSDIQALSRYVEDFVVAHPTQWYWVHRRWKRAFAAGAA
PLDEVALLTAG

Sequences:

>Translated_331_residues
MSNQAPRVPNRAAVAPVFAWWALQSAIFYGLTLLASSLPISLSRRFGAWGGAFIPRILPRRLDVAVDNISRALPFMQAHP
LWNPQNPPVEELAHQNFRNLGRNLVEISRLYHGREEGVLDVIELRGRENLDRAKGKGKGVVCFSGHCGNWEVMALALGSI
FGDGVVVARRQKNPFLEKMITRMRMRYNSRVLDKQGALRGILKALKKGELVGILADQAVLPEEGVLIEVMGRQAWASKAP
VIIARASGAAVVPVFIHREEGRQVITFHPEYRFSSDESEAGITSDIQALSRYVEDFVVAHPTQWYWVHRRWKRAFAAGAA
PLDEVALLTAG
>Mature_330_residues
SNQAPRVPNRAAVAPVFAWWALQSAIFYGLTLLASSLPISLSRRFGAWGGAFIPRILPRRLDVAVDNISRALPFMQAHPL
WNPQNPPVEELAHQNFRNLGRNLVEISRLYHGREEGVLDVIELRGRENLDRAKGKGKGVVCFSGHCGNWEVMALALGSIF
GDGVVVARRQKNPFLEKMITRMRMRYNSRVLDKQGALRGILKALKKGELVGILADQAVLPEEGVLIEVMGRQAWASKAPV
IIARASGAAVVPVFIHREEGRQVITFHPEYRFSSDESEAGITSDIQALSRYVEDFVVAHPTQWYWVHRRWKRAFAAGAAP
LDEVALLTAG

Specific function: Acylates the intermediate (KDO)2-lipid IVA to form (KDO)2-(lauroyl)-lipid IVA. Has 10 fold selectivity for lauroyl- ACP over myristoyl-ACP [H]

COG id: COG1560

COG function: function code M; Lauroyl/myristoyl acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the htrB/msbB family [H]

Homologues:

Organism=Escherichia coli, GI1787292, Length=287, Percent_Identity=26.4808362369338, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI87082094, Length=288, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004960
- InterPro:   IPR011920 [H]

Pfam domain/function: PF03279 Lip_A_acyltrans [H]

EC number: 2.3.1.-

Molecular weight: Translated: 36829; Mature: 36698

Theoretical pI: Translated: 10.47; Mature: 10.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNQAPRVPNRAAVAPVFAWWALQSAIFYGLTLLASSLPISLSRRFGAWGGAFIPRILPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHH
RLDVAVDNISRALPFMQAHPLWNPQNPPVEELAHQNFRNLGRNLVEISRLYHGREEGVLD
HHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VIELRGRENLDRAKGKGKGVVCFSGHCGNWEVMALALGSIFGDGVVVARRQKNPFLEKMI
HHHHCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
TRMRMRYNSRVLDKQGALRGILKALKKGELVGILADQAVLPEEGVLIEVMGRQAWASKAP
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCHHHCCCCC
VIIARASGAAVVPVFIHREEGRQVITFHPEYRFSSDESEAGITSDIQALSRYVEDFVVAH
EEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PTQWYWVHRRWKRAFAAGAAPLDEVALLTAG
CCHHHHHHHHHHHHHHCCCCCHHHEEEEECC
>Mature Secondary Structure 
SNQAPRVPNRAAVAPVFAWWALQSAIFYGLTLLASSLPISLSRRFGAWGGAFIPRILPR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHH
RLDVAVDNISRALPFMQAHPLWNPQNPPVEELAHQNFRNLGRNLVEISRLYHGREEGVLD
HHHHHHHHHHHHCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VIELRGRENLDRAKGKGKGVVCFSGHCGNWEVMALALGSIFGDGVVVARRQKNPFLEKMI
HHHHCCCCCHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
TRMRMRYNSRVLDKQGALRGILKALKKGELVGILADQAVLPEEGVLIEVMGRQAWASKAP
HHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEEEEECCHHHCCCCC
VIIARASGAAVVPVFIHREEGRQVITFHPEYRFSSDESEAGITSDIQALSRYVEDFVVAH
EEEEECCCCEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
PTQWYWVHRRWKRAFAAGAAPLDEVALLTAG
CCHHHHHHHHHHHHHHCCCCCHHHEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]