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The map label for this gene is gdh [H]

Identifier: 197116593

GI number: 197116593

Start: 228303

End: 229652

Strand: Direct

Name: gdh [H]

Synonym: Gbem_0193

Alternate gene names: 197116593

Gene position: 228303-229652 (Clockwise)

Preceding gene: 197116592

Following gene: 197116595

Centisome position: 4.95

GC content: 60.22

Gene sequence:

>1350_bases
ATGTCGCCACAGATTGACGAGAAAGTAGAGCCGATTTTTCAGGAAGTACTCAAGCGCAACCCCGGTGAGGTAGAGTTCCA
CCAGGCGGTACGTGAGGTCCTCGAGTCCCTCGGACCGGTGCTGGTGAAGCACCCGGAATTCGCCGAGCGCAAGATCATCG
AGAGGATCTGCGAGCCGGAGCGCCAGATCATCTTCAGAGTGCCCTGGCAGGACGACGGTGGGAACGTGCACATCAACCGC
GGCTTCCGCGTTGAATTCAACAGCTCCCTTGGCCCCTTCAAGGGCGGCCTGCGTTTCCATCCCTCGGTCTACCTCGGGAT
CATCAAGTTCCTGGGCTTCGAGCAGATCTTCAAGAACTCCTTGACCGGGCTTCCGATCGGCGGCGGCAAAGGCGGTTCCG
ACTTTGATCCCAAAGGGAAGTCCGACAACGAAATCATGCGTTTCTGCCAGAGCTTCATGACCGAACTCTACCGTCACCTG
GGCGAGCACACAGACGTTCCGGCGGGCGACATCGGGGTGGGCGGGCGCGAGATCGGCTACATGTTCGGCCAGTACAAGCG
CATCACCAACCGCTACGAGCCCGGCGTCCTCACCGGCAAAGGCCTCGACTGGGGCGGCTCGCTGGTGCGCACTGAGGCGA
CCGGTTACGGCGCCACCTTCTTCGTAGATGCCATGCTTAAGGTGCGCAAGGAATCCTTCGATGGCAAGGTCTGTACCGTT
TCCGGCTCCGGCAACGTGGCCATCTACACCATTGAGAAGATCCACCAGCTGGGCGGCAAGTGCATCAGCTGCTCCGACTC
CAACGGCGTGATCTACCACGAAAAAGGGCTCGACCTCAACCTGATCAAGCAGCTCAAGGAAGTGGAGCGCCGCCGCATCG
AGGACTACGCCAAGTACCACAAGGACGCAAAATACATCCCCAACGGCAAGATCTGGGATATCCCCTGCCAGGTGGCGATG
CCTTCCGCGACCCAGAACGAGATCAATGGCAAGGATGCAGCGACCCTGGTCAAAAATGGCTGCATCGCGGTCGGCGAAGG
CGCCAACATGCCGACTACTCCGGAAGGGGTCAAGGTGTTCCTCGAGGCGAATATCGCCTACGGCCCCGGTAAGGCAGCCA
ACGCAGGCGGCGTGGCTACCTCCGCTCTGGAGATGCAGCAGAACGCCTGCCGCGACGCATGGACCTTCGAATACACCGAG
CAGCGTCTGGCGCAGATCATGAAGAATATCCACGACACCTGCTATCAGACCGCCGAAGAGTACGGCACCCCGGGCAACTA
CGTGAACGGCGCCAACATTGCCGGCTTCATCAAGGTCGCCCAGGCAATGGTCGCACACGGCCTGATCTAG

Upstream 100 bases:

>100_bases
TGCATACAAATGTGGCACTTGGCGGGGGAGGTTTGCCCTGACAGTGTAAAACTGATTAAAGAGTGACCATAACACTCTTA
CCATGCCACTAGGAGGTAGT

Downstream 100 bases:

>100_bases
TAGCTAGCTGCGCCGTTTCAAAAAAGAAGGCCCGGCAGAGATGCCGGGCTTTCTTTTTTGCCTGCTCTTGCCTTGTTCCC
AGGGTCCTCCTTGAGAACAA

Product: glutamate dehydrogenase

Products: NA

Alternate protein names: NADP-GDH [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSPQIDEKVEPIFQEVLKRNPGEVEFHQAVREVLESLGPVLVKHPEFAERKIIERICEPERQIIFRVPWQDDGGNVHINR
GFRVEFNSSLGPFKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHL
GEHTDVPAGDIGVGGREIGYMFGQYKRITNRYEPGVLTGKGLDWGGSLVRTEATGYGATFFVDAMLKVRKESFDGKVCTV
SGSGNVAIYTIEKIHQLGGKCISCSDSNGVIYHEKGLDLNLIKQLKEVERRRIEDYAKYHKDAKYIPNGKIWDIPCQVAM
PSATQNEINGKDAATLVKNGCIAVGEGANMPTTPEGVKVFLEANIAYGPGKAANAGGVATSALEMQQNACRDAWTFEYTE
QRLAQIMKNIHDTCYQTAEEYGTPGNYVNGANIAGFIKVAQAMVAHGLI

Sequences:

>Translated_449_residues
MSPQIDEKVEPIFQEVLKRNPGEVEFHQAVREVLESLGPVLVKHPEFAERKIIERICEPERQIIFRVPWQDDGGNVHINR
GFRVEFNSSLGPFKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHL
GEHTDVPAGDIGVGGREIGYMFGQYKRITNRYEPGVLTGKGLDWGGSLVRTEATGYGATFFVDAMLKVRKESFDGKVCTV
SGSGNVAIYTIEKIHQLGGKCISCSDSNGVIYHEKGLDLNLIKQLKEVERRRIEDYAKYHKDAKYIPNGKIWDIPCQVAM
PSATQNEINGKDAATLVKNGCIAVGEGANMPTTPEGVKVFLEANIAYGPGKAANAGGVATSALEMQQNACRDAWTFEYTE
QRLAQIMKNIHDTCYQTAEEYGTPGNYVNGANIAGFIKVAQAMVAHGLI
>Mature_448_residues
SPQIDEKVEPIFQEVLKRNPGEVEFHQAVREVLESLGPVLVKHPEFAERKIIERICEPERQIIFRVPWQDDGGNVHINRG
FRVEFNSSLGPFKGGLRFHPSVYLGIIKFLGFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHLG
EHTDVPAGDIGVGGREIGYMFGQYKRITNRYEPGVLTGKGLDWGGSLVRTEATGYGATFFVDAMLKVRKESFDGKVCTVS
GSGNVAIYTIEKIHQLGGKCISCSDSNGVIYHEKGLDLNLIKQLKEVERRRIEDYAKYHKDAKYIPNGKIWDIPCQVAMP
SATQNEINGKDAATLVKNGCIAVGEGANMPTTPEGVKVFLEANIAYGPGKAANAGGVATSALEMQQNACRDAWTFEYTEQ
RLAQIMKNIHDTCYQTAEEYGTPGNYVNGANIAGFIKVAQAMVAHGLI

Specific function: Unknown

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=392, Percent_Identity=28.5714285714286, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI4885281, Length=392, Percent_Identity=28.5714285714286, Blast_Score=159, Evalue=5e-39,
Organism=Escherichia coli, GI1788059, Length=443, Percent_Identity=53.72460496614, Blast_Score=484, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17544676, Length=381, Percent_Identity=26.7716535433071, Blast_Score=125, Evalue=3e-29,
Organism=Saccharomyces cerevisiae, GI6319256, Length=454, Percent_Identity=48.8986784140969, Blast_Score=395, Evalue=1e-111,
Organism=Saccharomyces cerevisiae, GI6324951, Length=452, Percent_Identity=48.8938053097345, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45553475, Length=379, Percent_Identity=27.7044854881267, Blast_Score=138, Evalue=7e-33,
Organism=Drosophila melanogaster, GI45549226, Length=379, Percent_Identity=27.7044854881267, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24649283, Length=414, Percent_Identity=26.8115942028986, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI24649460, Length=313, Percent_Identity=27.7955271565495, Blast_Score=112, Evalue=6e-25,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR014362
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.4 [H]

Molecular weight: Translated: 49483; Mature: 49352

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPQIDEKVEPIFQEVLKRNPGEVEFHQAVREVLESLGPVLVKHPEFAERKIIERICEPE
CCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCC
RQIIFRVPWQDDGGNVHINRGFRVEFNSSLGPFKGGLRFHPSVYLGIIKFLGFEQIFKNS
EEEEEECCCCCCCCEEEEECCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCC
LTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHLGEHTDVPAGDIGVGGREIGY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNRYEPGVLTGKGLDWGGSLVRTEATGYGATFFVDAMLKVRKESFDGKVCTV
HHHHHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
SGSGNVAIYTIEKIHQLGGKCISCSDSNGVIYHEKGLDLNLIKQLKEVERRRIEDYAKYH
ECCCCEEEEEHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KDAKYIPNGKIWDIPCQVAMPSATQNEINGKDAATLVKNGCIAVGEGANMPTTPEGVKVF
CCCCCCCCCCEEECCCCEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCEEEE
LEANIAYGPGKAANAGGVATSALEMQQNACRDAWTFEYTEQRLAQIMKNIHDTCYQTAEE
EEECEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGTPGNYVNGANIAGFIKVAQAMVAHGLI
HCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SPQIDEKVEPIFQEVLKRNPGEVEFHQAVREVLESLGPVLVKHPEFAERKIIERICEPE
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHCCCC
RQIIFRVPWQDDGGNVHINRGFRVEFNSSLGPFKGGLRFHPSVYLGIIKFLGFEQIFKNS
EEEEEECCCCCCCCEEEEECCEEEEECCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHCC
LTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMTELYRHLGEHTDVPAGDIGVGGREIGY
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHH
MFGQYKRITNRYEPGVLTGKGLDWGGSLVRTEATGYGATFFVDAMLKVRKESFDGKVCTV
HHHHHHHHHCCCCCCCEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEE
SGSGNVAIYTIEKIHQLGGKCISCSDSNGVIYHEKGLDLNLIKQLKEVERRRIEDYAKYH
ECCCCEEEEEHHHHHHHCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
KDAKYIPNGKIWDIPCQVAMPSATQNEINGKDAATLVKNGCIAVGEGANMPTTPEGVKVF
CCCCCCCCCCEEECCCCEECCCCCCCCCCCHHHHHHHHCCCEEECCCCCCCCCCCCEEEE
LEANIAYGPGKAANAGGVATSALEMQQNACRDAWTFEYTEQRLAQIMKNIHDTCYQTAEE
EEECEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YGTPGNYVNGANIAGFIKVAQAMVAHGLI
HCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]