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The map label for this gene is pilT-2 [H]

Identifier: 197116592

GI number: 197116592

Start: 226858

End: 227928

Strand: Direct

Name: pilT-2 [H]

Synonym: Gbem_0192

Alternate gene names: 197116592

Gene position: 226858-227928 (Clockwise)

Preceding gene: 197116591

Following gene: 197116593

Centisome position: 4.92

GC content: 59.48

Gene sequence:

>1071_bases
ATGGATTCCAAGATCTTCGTCCAAATACTTGAAATAGCCTTCAGCAAAAAGGTTTCCGACGTGCATTTCGAGGTGGACAA
TCCCCCGTTTTTCCGCGGCAAGGGACAGATCATCCGCTCCAAGCTCCCCAGCCTCACCTCCGAGGATACGGAATTCATCG
CGTCGCAGATCATGGCACACAACAACCGCCGCTGGGAGGCCGACCAGAAGGAATACGACACCTCCTTCTCCCTGCCGAGC
GGCGCGCGCTTCCGCGTCAGTATCTTCAAGCAGCGCGGGCACTTCGGCATCATCATGAGGGTCATTCCCGCCAACATCGG
CACCTTTGAAGAACTCCGCCTGCCGGCTGTGCTTGGGGAGGTTGCCAAGGCACCCAACGGACTCATCCTGGTGACCGGCC
CGACCGGCAACGGCAAATCGACCACCCTCGCCTCGATACTGCGCTACATCAACGAAAACCTCGCCTACAACTGCATCACC
ATCGAGGACCCGATCGAATTCCTGTTCAAGTCCGAGAAGAGCTGCATCATCCAGCGCGAGGTGGGGATCGACACCGACAG
CTTCAGTTCTGCGGTAAAAGCCGCTTTGCGCATGGACCCGGATCTGATCATGGTCGGCGAGATGCGCGACACCGCCACCA
TCGAATCCTGCCTGATGGCGGCCGAGACCGGCCACCTGGTTCTCTCCACCCTGCACACCCAGGGTGCGGTATCGACCATC
AACAGGATCGTCGGGCACTTCCCTCCCGACGCCCAGGAGATCGTGCGCCAAAGGCTTGCGGACATCCTGGTCGCCACCGT
CTCGATACGCCTGGTGATGAACAAGACCGGAGAGGCGATCCTTCCGGTCCTCGAGATCATGCGCGCCACCACCACCATCC
AAAGCTGCATCAGGGAGGGGAGGCTGGACGAGATCGAGGCGCACATGGAGAACGGCCGCAGCCAATACCAGATGCAGACC
TTCGACCAGCACCTGATCCAGTTGCACGAGCAGGAGCAGATCACCATGGAGGACGCCAAGCGGCTTTCCCACTCCATGGA
CCTGGAACGGAAACTGATGTTCACCAACTAG

Upstream 100 bases:

>100_bases
GCATGCGGGGCTCATCATAGACAACGTGCTCTACCGCAAAAGGTGCGCGCAAAAATAAGCAGCAAGTTTCGATGTCATCC
ATTCAGTTACAGGGGGGACC

Downstream 100 bases:

>100_bases
CCTCGCCATCGCCACGAGAAAAGGGGGGGTGCCGTTTATTGCGGCACCCCCCCCTTTTTTTTTATTTGCGCCTCCCATTT
TTCCCAGCTGTCATACCTGC

Product: twitching motility pilus retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MDSKIFVQILEIAFSKKVSDVHFEVDNPPFFRGKGQIIRSKLPSLTSEDTEFIASQIMAHNNRRWEADQKEYDTSFSLPS
GARFRVSIFKQRGHFGIIMRVIPANIGTFEELRLPAVLGEVAKAPNGLILVTGPTGNGKSTTLASILRYINENLAYNCIT
IEDPIEFLFKSEKSCIIQREVGIDTDSFSSAVKAALRMDPDLIMVGEMRDTATIESCLMAAETGHLVLSTLHTQGAVSTI
NRIVGHFPPDAQEIVRQRLADILVATVSIRLVMNKTGEAILPVLEIMRATTTIQSCIREGRLDEIEAHMENGRSQYQMQT
FDQHLIQLHEQEQITMEDAKRLSHSMDLERKLMFTN

Sequences:

>Translated_356_residues
MDSKIFVQILEIAFSKKVSDVHFEVDNPPFFRGKGQIIRSKLPSLTSEDTEFIASQIMAHNNRRWEADQKEYDTSFSLPS
GARFRVSIFKQRGHFGIIMRVIPANIGTFEELRLPAVLGEVAKAPNGLILVTGPTGNGKSTTLASILRYINENLAYNCIT
IEDPIEFLFKSEKSCIIQREVGIDTDSFSSAVKAALRMDPDLIMVGEMRDTATIESCLMAAETGHLVLSTLHTQGAVSTI
NRIVGHFPPDAQEIVRQRLADILVATVSIRLVMNKTGEAILPVLEIMRATTTIQSCIREGRLDEIEAHMENGRSQYQMQT
FDQHLIQLHEQEQITMEDAKRLSHSMDLERKLMFTN
>Mature_356_residues
MDSKIFVQILEIAFSKKVSDVHFEVDNPPFFRGKGQIIRSKLPSLTSEDTEFIASQIMAHNNRRWEADQKEYDTSFSLPS
GARFRVSIFKQRGHFGIIMRVIPANIGTFEELRLPAVLGEVAKAPNGLILVTGPTGNGKSTTLASILRYINENLAYNCIT
IEDPIEFLFKSEKSCIIQREVGIDTDSFSSAVKAALRMDPDLIMVGEMRDTATIESCLMAAETGHLVLSTLHTQGAVSTI
NRIVGHFPPDAQEIVRQRLADILVATVSIRLVMNKTGEAILPVLEIMRATTTIQSCIREGRLDEIEAHMENGRSQYQMQT
FDQHLIQLHEQEQITMEDAKRLSHSMDLERKLMFTN

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=316, Percent_Identity=37.6582278481013, Blast_Score=217, Evalue=9e-58,
Organism=Escherichia coli, GI1786296, Length=281, Percent_Identity=29.8932384341637, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1789723, Length=120, Percent_Identity=50, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39996; Mature: 39996

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSKIFVQILEIAFSKKVSDVHFEVDNPPFFRGKGQIIRSKLPSLTSEDTEFIASQIMAH
CCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHC
NNRRWEADQKEYDTSFSLPSGARFRVSIFKQRGHFGIIMRVIPANIGTFEELRLPAVLGE
CCCCCCCCHHHCCCCCCCCCCCEEEEEEEHHCCCEEEEEEEECCCCCCHHHHHHHHHHHH
VAKAPNGLILVTGPTGNGKSTTLASILRYINENLAYNCITIEDPIEFLFKSEKSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCEEEECC
VGIDTDSFSSAVKAALRMDPDLIMVGEMRDTATIESCLMAAETGHLVLSTLHTQGAVSTI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
NRIVGHFPPDAQEIVRQRLADILVATVSIRLVMNKTGEAILPVLEIMRATTTIQSCIREG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
RLDEIEAHMENGRSQYQMQTFDQHLIQLHEQEQITMEDAKRLSHSMDLERKLMFTN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure
MDSKIFVQILEIAFSKKVSDVHFEVDNPPFFRGKGQIIRSKLPSLTSEDTEFIASQIMAH
CCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHC
NNRRWEADQKEYDTSFSLPSGARFRVSIFKQRGHFGIIMRVIPANIGTFEELRLPAVLGE
CCCCCCCCHHHCCCCCCCCCCCEEEEEEEHHCCCEEEEEEEECCCCCCHHHHHHHHHHHH
VAKAPNGLILVTGPTGNGKSTTLASILRYINENLAYNCITIEDPIEFLFKSEKSCIIQRE
HHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHCCEEEEEEEHHHHHHHHCCCCCEEEECC
VGIDTDSFSSAVKAALRMDPDLIMVGEMRDTATIESCLMAAETGHLVLSTLHTQGAVSTI
CCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHCCHHHHHH
NRIVGHFPPDAQEIVRQRLADILVATVSIRLVMNKTGEAILPVLEIMRATTTIQSCIREG
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHCC
RLDEIEAHMENGRSQYQMQTFDQHLIQLHEQEQITMEDAKRLSHSMDLERKLMFTN
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8100347 [H]