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The map label for this gene is nuoL-2 [H]

Identifier: 197116569

GI number: 197116569

Start: 199020

End: 200891

Strand: Reverse

Name: nuoL-2 [H]

Synonym: Gbem_0169

Alternate gene names: 197116569

Gene position: 200891-199020 (Counterclockwise)

Preceding gene: 197116570

Following gene: 197116568

Centisome position: 4.35

GC content: 64.69

Gene sequence:

>1872_bases
ATGCAAACCTACCTATCCCTCATGCTTTTATTCCCGCTCTTCGGCTCTGTCGTCTGCGCCCTGGCGGGACGGAAACTGCC
GCGCGTGCTGGTGGAGGTACTCGCCTGCGCGACGGTGTGGGGGAGCTTCGCCTGCGCGGCGCTGGCGGCCGCAGCCTATG
GCGCGCCGCAAACGGTCATTATCGCGCGATGGTTCGCTTCATCGGACCTCCAGATCCCGGTCTCGCTCTACCTCGATCCT
CTTTCGCTCTCGCTTTCCCTGATGGTCGGCTTTGTCTGCGGCCTGATCCACATCTACTCGGTCTTTTACATGCGAGGCGA
CGAGGACTACGCGCGCTACTTCGCCTTATTGAACCTCTTCGTCTTCGCCATGCTCGCCCTGGTCCTGGCAGAAAGCCTGC
CGCTTCTCTACCTCGGCTGGGAAGGGGTCGGGTTCTGCTCGTACCTCCTCATCGGCTTCTGGTACCGGGACAAGGACAAC
GCCGACGCCGGCAGGAAGGCGTTCATCACCACCCGGATCGGGGATACGGCGCTCATGGTCGCCCTGGCCTGGCTGTTCCA
GCTCTTCGGCACCCTCTCGATCACGCAACTAAACGGCATGGGCTTTCTCATGCCCGGCTCGATCATCACCGCCCTCGGCA
TCCTCTTCCTGATCGCCGCGATGGGTAAATCGGCCCAGGTCCCGCTCATGGTCTGGCTCCCCGACGCCATGGCCGGCCCC
ACGCCGGTCTCGGCTATGATCCACGCGGCGACCATGGTGACGGCCGGCGTCTACCTGCTGGCGCGCATGCTGCCGCTGAT
CGGTTCGTCCCCGACGGCCATGGCCGCCATCGCCGTCACCGGCGGCGTCACCGCTTTTTACGGGGCCACCTGCGCCCTGG
CGCAGCGCGACCTGAAGCGCGTGCTGGCCTTTTCCACCATCAGCCAGATCGGCTACATGATGCTCGGGGTCGGCGCGGGC
GCCGTCACCGCCGCAACCTTCCACCTGCTCGTGCACGCCTTCTTCAAGGCGCTCCTGTTCCTCGGCGCTGGCTGCGTCAT
AACCGCCATGCACCACGAGCAGGACATCTTCAAGATGGGGGGCCTGCGGCGCACCCTCCCGGTCACCTTCTGGAGCTTCC
TCGCCGGAGCCGCCTGCCTGGCCGGGCTACCCCTGACCGGCGGGTTCTTCAGCAAGGACAGCATCCTCTTGGCCGTCTGG
CACAAGGGGGGGCTCTTGTACCAGTCCCTGTACCTGTTCGCGCTCCTCACCGCGCTCATCACCGCCTACTACACCTTCCG
CATGGTCTACCTCGTCTTCGGCGGCTCCTCCCATGCCGCTCATGAGACAGGCGGGCTGAAGACCATGCAGGCAGTCTTGA
TCCCGCTGGCGCTCTTGGCCCTCTTCGGCGGCATGCTTGACCTTCCCGCCTACTTGGGCGAAGGGTTCCTGGGCAGCCTG
TTCGCCGGGGTCCCGGGAGGGAAAATGGTCGAGGGTCCGGGCAATGAGGAAATGGCGCTGCAGGCGATAGCAGGTGCGCT
GGCCCTGGCTGGGCTTGTGGCCGCGCACGTAAGGTACGGCCGGGGACGGGAGCAACGGATCGAGGAGGCAGGGGACGAGT
CCCCGCTCTTCGCGTTCCTTTTGAAGGGATGGTACTTCGACGCCTTGTACGACATGCTCCTGGTCCGCCCTTACCAGGCG
GCAGCCAGGGTTCTCTGGCAAAAGGTAGACGAAAGGATGATAGACGACTCGCTGGACCGGCTGGCGGGCGGGGTTGGCTG
GACCGGCACCTGGCTTGGGCGCTGGAGCACCGGCCGCGTCTCGATTTATCTGCTTAGTTTCGCCGCCGGGGTCGCTTTCA
TGATCGGCTACCTCGCCTGGATTTTCATCTAA

Upstream 100 bases:

>100_bases
TGAAGGCGGAACACAGAGGTCTCGGAGGTCCACGGAGGTTTTTCACCTCCCCCTCCCGCGAGGGGAGGGGGAACTGAAAG
GCTAAGAACAAGGAGTATCG

Downstream 100 bases:

>100_bases
CACGGGACACCTGCGATGACACCTGACTTCCCGATACTTTCCGTGCTGGTCTTTCTGCCGCTTGCCGCGTCGCTGCTCCT
TGTGGCGTTGCGCCCTGCGC

Product: NADH dehydrogenase I subunit L lipoprotein

Products: NA

Alternate protein names: NADH dehydrogenase I subunit L; NDH-1 subunit L; NUO12 [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MQTYLSLMLLFPLFGSVVCALAGRKLPRVLVEVLACATVWGSFACAALAAAAYGAPQTVIIARWFASSDLQIPVSLYLDP
LSLSLSLMVGFVCGLIHIYSVFYMRGDEDYARYFALLNLFVFAMLALVLAESLPLLYLGWEGVGFCSYLLIGFWYRDKDN
ADAGRKAFITTRIGDTALMVALAWLFQLFGTLSITQLNGMGFLMPGSIITALGILFLIAAMGKSAQVPLMVWLPDAMAGP
TPVSAMIHAATMVTAGVYLLARMLPLIGSSPTAMAAIAVTGGVTAFYGATCALAQRDLKRVLAFSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVITAMHHEQDIFKMGGLRRTLPVTFWSFLAGAACLAGLPLTGGFFSKDSILLAVW
HKGGLLYQSLYLFALLTALITAYYTFRMVYLVFGGSSHAAHETGGLKTMQAVLIPLALLALFGGMLDLPAYLGEGFLGSL
FAGVPGGKMVEGPGNEEMALQAIAGALALAGLVAAHVRYGRGREQRIEEAGDESPLFAFLLKGWYFDALYDMLLVRPYQA
AARVLWQKVDERMIDDSLDRLAGGVGWTGTWLGRWSTGRVSIYLLSFAAGVAFMIGYLAWIFI

Sequences:

>Translated_623_residues
MQTYLSLMLLFPLFGSVVCALAGRKLPRVLVEVLACATVWGSFACAALAAAAYGAPQTVIIARWFASSDLQIPVSLYLDP
LSLSLSLMVGFVCGLIHIYSVFYMRGDEDYARYFALLNLFVFAMLALVLAESLPLLYLGWEGVGFCSYLLIGFWYRDKDN
ADAGRKAFITTRIGDTALMVALAWLFQLFGTLSITQLNGMGFLMPGSIITALGILFLIAAMGKSAQVPLMVWLPDAMAGP
TPVSAMIHAATMVTAGVYLLARMLPLIGSSPTAMAAIAVTGGVTAFYGATCALAQRDLKRVLAFSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVITAMHHEQDIFKMGGLRRTLPVTFWSFLAGAACLAGLPLTGGFFSKDSILLAVW
HKGGLLYQSLYLFALLTALITAYYTFRMVYLVFGGSSHAAHETGGLKTMQAVLIPLALLALFGGMLDLPAYLGEGFLGSL
FAGVPGGKMVEGPGNEEMALQAIAGALALAGLVAAHVRYGRGREQRIEEAGDESPLFAFLLKGWYFDALYDMLLVRPYQA
AARVLWQKVDERMIDDSLDRLAGGVGWTGTWLGRWSTGRVSIYLLSFAAGVAFMIGYLAWIFI
>Mature_623_residues
MQTYLSLMLLFPLFGSVVCALAGRKLPRVLVEVLACATVWGSFACAALAAAAYGAPQTVIIARWFASSDLQIPVSLYLDP
LSLSLSLMVGFVCGLIHIYSVFYMRGDEDYARYFALLNLFVFAMLALVLAESLPLLYLGWEGVGFCSYLLIGFWYRDKDN
ADAGRKAFITTRIGDTALMVALAWLFQLFGTLSITQLNGMGFLMPGSIITALGILFLIAAMGKSAQVPLMVWLPDAMAGP
TPVSAMIHAATMVTAGVYLLARMLPLIGSSPTAMAAIAVTGGVTAFYGATCALAQRDLKRVLAFSTISQIGYMMLGVGAG
AVTAATFHLLVHAFFKALLFLGAGCVITAMHHEQDIFKMGGLRRTLPVTFWSFLAGAACLAGLPLTGGFFSKDSILLAVW
HKGGLLYQSLYLFALLTALITAYYTFRMVYLVFGGSSHAAHETGGLKTMQAVLIPLALLALFGGMLDLPAYLGEGFLGSL
FAGVPGGKMVEGPGNEEMALQAIAGALALAGLVAAHVRYGRGREQRIEEAGDESPLFAFLLKGWYFDALYDMLLVRPYQA
AARVLWQKVDERMIDDSLDRLAGGVGWTGTWLGRWSTGRVSIYLLSFAAGVAFMIGYLAWIFI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1009

COG function: function code CP; NADH:ubiquinone oxidoreductase subunit 5 (chain L)/Multisubunit Na+/H+ antiporter, MnhA subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=465, Percent_Identity=35.4838709677419, Blast_Score=258, Evalue=1e-68,
Organism=Escherichia coli, GI1788614, Length=617, Percent_Identity=40.8427876823339, Blast_Score=377, Evalue=1e-105,
Organism=Escherichia coli, GI1788829, Length=401, Percent_Identity=33.4164588528678, Blast_Score=158, Evalue=8e-40,
Organism=Escherichia coli, GI1788827, Length=340, Percent_Identity=29.4117647058824, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1788831, Length=343, Percent_Identity=28.5714285714286, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI1788613, Length=447, Percent_Identity=27.7404921700224, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI2367154, Length=342, Percent_Identity=29.5321637426901, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI145693160, Length=312, Percent_Identity=26.9230769230769, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR001516
- InterPro:   IPR003945
- InterPro:   IPR018393 [H]

Pfam domain/function: PF00361 Oxidored_q1; PF00662 Oxidored_q1_N [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 66943; Mature: 66943

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTYLSLMLLFPLFGSVVCALAGRKLPRVLVEVLACATVWGSFACAALAAAAYGAPQTVI
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IARWFASSDLQIPVSLYLDPLSLSLSLMVGFVCGLIHIYSVFYMRGDEDYARYFALLNLF
EEEEECCCCCEEEHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFAMLALVLAESLPLLYLGWEGVGFCSYLLIGFWYRDKDNADAGRKAFITTRIGDTALMV
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHH
ALAWLFQLFGTLSITQLNGMGFLMPGSIITALGILFLIAAMGKSAQVPLMVWLPDAMAGP
HHHHHHHHHCCCEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TPVSAMIHAATMVTAGVYLLARMLPLIGSSPTAMAAIAVTGGVTAFYGATCALAQRDLKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VLAFSTISQIGYMMLGVGAGAVTAATFHLLVHAFFKALLFLGAGCVITAMHHEQDIFKMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHC
GLRRTLPVTFWSFLAGAACLAGLPLTGGFFSKDSILLAVWHKGGLLYQSLYLFALLTALI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TAYYTFRMVYLVFGGSSHAAHETGGLKTMQAVLIPLALLALFGGMLDLPAYLGEGFLGSL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FAGVPGGKMVEGPGNEEMALQAIAGALALAGLVAAHVRYGRGREQRIEEAGDESPLFAFL
HHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHH
LKGWYFDALYDMLLVRPYQAAARVLWQKVDERMIDDSLDRLAGGVGWTGTWLGRWSTGRV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
SIYLLSFAAGVAFMIGYLAWIFI
EEHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MQTYLSLMLLFPLFGSVVCALAGRKLPRVLVEVLACATVWGSFACAALAAAAYGAPQTVI
CHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
IARWFASSDLQIPVSLYLDPLSLSLSLMVGFVCGLIHIYSVFYMRGDEDYARYFALLNLF
EEEEECCCCCEEEHHEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VFAMLALVLAESLPLLYLGWEGVGFCSYLLIGFWYRDKDNADAGRKAFITTRIGDTALMV
HHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHHHHH
ALAWLFQLFGTLSITQLNGMGFLMPGSIITALGILFLIAAMGKSAQVPLMVWLPDAMAGP
HHHHHHHHHCCCEEEEECCCCEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCC
TPVSAMIHAATMVTAGVYLLARMLPLIGSSPTAMAAIAVTGGVTAFYGATCALAQRDLKR
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VLAFSTISQIGYMMLGVGAGAVTAATFHLLVHAFFKALLFLGAGCVITAMHHEQDIFKMG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHC
GLRRTLPVTFWSFLAGAACLAGLPLTGGFFSKDSILLAVWHKGGLLYQSLYLFALLTALI
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHH
TAYYTFRMVYLVFGGSSHAAHETGGLKTMQAVLIPLALLALFGGMLDLPAYLGEGFLGSL
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FAGVPGGKMVEGPGNEEMALQAIAGALALAGLVAAHVRYGRGREQRIEEAGDESPLFAFL
HHCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHH
LKGWYFDALYDMLLVRPYQAAARVLWQKVDERMIDDSLDRLAGGVGWTGTWLGRWSTGRV
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
SIYLLSFAAGVAFMIGYLAWIFI
EEHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7690854; 9205837; 9278503 [H]