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The map label for this gene is nuoM-2 [H]

Identifier: 197116568

GI number: 197116568

Start: 197520

End: 199004

Strand: Reverse

Name: nuoM-2 [H]

Synonym: Gbem_0168

Alternate gene names: 197116568

Gene position: 199004-197520 (Counterclockwise)

Preceding gene: 197116569

Following gene: 197116567

Centisome position: 4.31

GC content: 62.29

Gene sequence:

>1485_bases
ATGACACCTGACTTCCCGATACTTTCCGTGCTGGTCTTTCTGCCGCTTGCCGCGTCGCTGCTCCTTGTGGCGTTGCGCCC
TGCGCAGGCGCTGGCGCGGGGTTTCGCCCTGGCAGTGGCCCTGGTGGAGCTGGCCCTCGCGGCTTTCCCGCTTCTCGGCA
CCTCGCTGTCCGCTACCTACCCTAGTGCGCCCAAGGGCTTCTTCCTGTTCGAGGACCTCCCCTGGATCGAGCGTTTCGGC
ATCCGTTACACCCTCGGCATGGATGGCATCAGCCAGATCATGGTGCTCCTGACCTCGTTCATTACCGTCGTCGCCGTCCT
CATCTCATGGAAACAGATCAAGGAGCGGGCGCCTGTTTACTATGCCCTGCTGCTCGCCCTGCAGTGCAGCATCCAGGGCG
TATTCCTCAGCCTCGACCTGTTTCTTTTCTACCTTTTCTTCGAGGCGATGCTGATCCCCATCGTGTTCCTCTCCGGCGTC
TGGGGTGATGGGCGACCGGTTTATTCGGCCATCAAGTTCTTTCTCTATACCTTCGTCGGTTCCCTGCTCATGCTGGTCTC
CATCATCACGCTCTACCTGATGCACGGCGCGCAGACAGGGAGCTACACCTTCGCCCTCCCCGCTTTGGTGCAGACCAGCG
TCCCCTATGGCCTTGCCCTCTGGCTTTTTGCCGGGTTCCTGGTGGCCTTCGCGGTGAAGATCCCGCTTTTCCCGCTGCAC
AGCTGGCAGCCCGACGCCTACGCCGATGCCCCCATCGCCGGGACCCTGATCCTCGCCAGCCTGCTTTCCAAGACCGGCGC
CTACGGCCTGATCCGTTTCGCCTATCCGCTTTTTCCCGAAGCCGCCCGCGCCTTCACCCCCGTCCTCTACCTGGCCGCCG
TCTTCGTGATCAGCTATGGCGCCTGGATTGCCTTCGCCCAAAAGGACATGAAGCGGCTGGTGGCGTACTCCAGCCTCAGC
CACATGGGTTTCATCGCCCTGGGGATAGCCGCCTGGAGCCCGCTAGCCCTGTCCGGAGCGCTTTTGCAGATGGTGAACCA
CGCCGTCACCACCGGGGCCCTCTTCGTTGTGGTGGGAATGCTGGACGAGCGTGCCGGCAGCAGGGATCTCCCCGCCTACG
GCGGCATCTGGGAAAAGGTCCCGGCGCTGTCGTTCTTTTTCCTGTTCTTCTGCATGGCCTCCGCCGGGCTGCCAGGGTTG
AACAACTTCGTCGGTGAGTTCTTGGTGCTGGTGGGGGCGTTCAAGGTTGCGCCCGCAGCCGCCCTGGGCGCGTTTCTGGG
TATCGTGCTGACGCTGATCTACACGGTGAGGCTGGTGCAGGAGGTGTTGTTCGTCAGGGAGAGGAAGCCGCTAGAGTTGA
AGGATCTGACCATGAGAGAAGCCGGCGTCCTGGCCATCCTCGCGCTGGTAGCCGTGTACCTGGGCGTTCATCCCAGCCCC
GTGCTGGAACTGTTTAAAGGGCCGATCGCGTTGCTGGTCAAGTAA

Upstream 100 bases:

>100_bases
CACCGGCCGCGTCTCGATTTATCTGCTTAGTTTCGCCGCCGGGGTCGCTTTCATGATCGGCTACCTCGCCTGGATTTTCA
TCTAACACGGGACACCTGCG

Downstream 100 bases:

>100_bases
AGGCACTCACCACAGAGGAACACAGAGGAACCACAGAGGAAAAACCTCATGGATCCAAATCTTGTCACCGGTCATATCAT
TGATGCCGCCATGAAGGTTC

Product: NADH dehydrogenase I subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M; NUO13 [H]

Number of amino acids: Translated: 494; Mature: 493

Protein sequence:

>494_residues
MTPDFPILSVLVFLPLAASLLLVALRPAQALARGFALAVALVELALAAFPLLGTSLSATYPSAPKGFFLFEDLPWIERFG
IRYTLGMDGISQIMVLLTSFITVVAVLISWKQIKERAPVYYALLLALQCSIQGVFLSLDLFLFYLFFEAMLIPIVFLSGV
WGDGRPVYSAIKFFLYTFVGSLLMLVSIITLYLMHGAQTGSYTFALPALVQTSVPYGLALWLFAGFLVAFAVKIPLFPLH
SWQPDAYADAPIAGTLILASLLSKTGAYGLIRFAYPLFPEAARAFTPVLYLAAVFVISYGAWIAFAQKDMKRLVAYSSLS
HMGFIALGIAAWSPLALSGALLQMVNHAVTTGALFVVVGMLDERAGSRDLPAYGGIWEKVPALSFFFLFFCMASAGLPGL
NNFVGEFLVLVGAFKVAPAAALGAFLGIVLTLIYTVRLVQEVLFVRERKPLELKDLTMREAGVLAILALVAVYLGVHPSP
VLELFKGPIALLVK

Sequences:

>Translated_494_residues
MTPDFPILSVLVFLPLAASLLLVALRPAQALARGFALAVALVELALAAFPLLGTSLSATYPSAPKGFFLFEDLPWIERFG
IRYTLGMDGISQIMVLLTSFITVVAVLISWKQIKERAPVYYALLLALQCSIQGVFLSLDLFLFYLFFEAMLIPIVFLSGV
WGDGRPVYSAIKFFLYTFVGSLLMLVSIITLYLMHGAQTGSYTFALPALVQTSVPYGLALWLFAGFLVAFAVKIPLFPLH
SWQPDAYADAPIAGTLILASLLSKTGAYGLIRFAYPLFPEAARAFTPVLYLAAVFVISYGAWIAFAQKDMKRLVAYSSLS
HMGFIALGIAAWSPLALSGALLQMVNHAVTTGALFVVVGMLDERAGSRDLPAYGGIWEKVPALSFFFLFFCMASAGLPGL
NNFVGEFLVLVGAFKVAPAAALGAFLGIVLTLIYTVRLVQEVLFVRERKPLELKDLTMREAGVLAILALVAVYLGVHPSP
VLELFKGPIALLVK
>Mature_493_residues
TPDFPILSVLVFLPLAASLLLVALRPAQALARGFALAVALVELALAAFPLLGTSLSATYPSAPKGFFLFEDLPWIERFGI
RYTLGMDGISQIMVLLTSFITVVAVLISWKQIKERAPVYYALLLALQCSIQGVFLSLDLFLFYLFFEAMLIPIVFLSGVW
GDGRPVYSAIKFFLYTFVGSLLMLVSIITLYLMHGAQTGSYTFALPALVQTSVPYGLALWLFAGFLVAFAVKIPLFPLHS
WQPDAYADAPIAGTLILASLLSKTGAYGLIRFAYPLFPEAARAFTPVLYLAAVFVISYGAWIAFAQKDMKRLVAYSSLSH
MGFIALGIAAWSPLALSGALLQMVNHAVTTGALFVVVGMLDERAGSRDLPAYGGIWEKVPALSFFFLFFCMASAGLPGLN
NFVGEFLVLVGAFKVAPAAALGAFLGIVLTLIYTVRLVQEVLFVRERKPLELKDLTMREAGVLAILALVAVYLGVHPSPV
LELFKGPIALLVK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=326, Percent_Identity=33.4355828220859, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1788613, Length=420, Percent_Identity=42.3809523809524, Blast_Score=340, Evalue=1e-94,
Organism=Escherichia coli, GI1788831, Length=362, Percent_Identity=27.9005524861878, Blast_Score=115, Evalue=7e-27,
Organism=Escherichia coli, GI1788827, Length=282, Percent_Identity=28.3687943262411, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1788829, Length=348, Percent_Identity=29.5977011494253, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI2367154, Length=333, Percent_Identity=25.8258258258258, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI145693160, Length=418, Percent_Identity=25.3588516746411, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788614, Length=391, Percent_Identity=27.1099744245524, Blast_Score=69, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 53554; Mature: 53423

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDFPILSVLVFLPLAASLLLVALRPAQALARGFALAVALVELALAAFPLLGTSLSATY
CCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSAPKGFFLFEDLPWIERFGIRYTLGMDGISQIMVLLTSFITVVAVLISWKQIKERAPVY
CCCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
YALLLALQCSIQGVFLSLDLFLFYLFFEAMLIPIVFLSGVWGDGRPVYSAIKFFLYTFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SLLMLVSIITLYLMHGAQTGSYTFALPALVQTSVPYGLALWLFAGFLVAFAVKIPLFPLH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SWQPDAYADAPIAGTLILASLLSKTGAYGLIRFAYPLFPEAARAFTPVLYLAAVFVISYG
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AWIAFAQKDMKRLVAYSSLSHMGFIALGIAAWSPLALSGALLQMVNHAVTTGALFVVVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDERAGSRDLPAYGGIWEKVPALSFFFLFFCMASAGLPGLNNFVGEFLVLVGAFKVAPAA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALGAFLGIVLTLIYTVRLVQEVLFVRERKPLELKDLTMREAGVLAILALVAVYLGVHPSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLELFKGPIALLVK
HHHHHCCCHHHHCC
>Mature Secondary Structure 
TPDFPILSVLVFLPLAASLLLVALRPAQALARGFALAVALVELALAAFPLLGTSLSATY
CCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PSAPKGFFLFEDLPWIERFGIRYTLGMDGISQIMVLLTSFITVVAVLISWKQIKERAPVY
CCCCCCEEEECCCCHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
YALLLALQCSIQGVFLSLDLFLFYLFFEAMLIPIVFLSGVWGDGRPVYSAIKFFLYTFVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
SLLMLVSIITLYLMHGAQTGSYTFALPALVQTSVPYGLALWLFAGFLVAFAVKIPLFPLH
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCC
SWQPDAYADAPIAGTLILASLLSKTGAYGLIRFAYPLFPEAARAFTPVLYLAAVFVISYG
CCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
AWIAFAQKDMKRLVAYSSLSHMGFIALGIAAWSPLALSGALLQMVNHAVTTGALFVVVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDERAGSRDLPAYGGIWEKVPALSFFFLFFCMASAGLPGLNNFVGEFLVLVGAFKVAPAA
HHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ALGAFLGIVLTLIYTVRLVQEVLFVRERKPLELKDLTMREAGVLAILALVAVYLGVHPSP
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHH
VLELFKGPIALLVK
HHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]