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The map label for this gene is purT [H]

Identifier: 197116560

GI number: 197116560

Start: 191810

End: 192988

Strand: Direct

Name: purT [H]

Synonym: Gbem_0160

Alternate gene names: 197116560

Gene position: 191810-192988 (Clockwise)

Preceding gene: 197116559

Following gene: 197116561

Centisome position: 4.16

GC content: 65.99

Gene sequence:

>1179_bases
ATGATAGGAACGCCGCTGAAAAACAGTGCGACGAGGATGATGCTGCTGGGCTCAGGCGAGCTAGGCAAGGAAGTGGCCCT
GGAGGCCCAGAGGCTGGGGATCGAGGTGATAGCGGTGGACCGTTATGCCGACGCCCCGGCGATGCAGGTCGCCCACCGAA
GCCACGTGATCGATATGCTCGACCGCGAACAACTGGACCGGGTGGTCCGCCTGGAGAATCCGTCGCTGATCGTGCCGGAG
ATCGAGGCGATCAACACCGTCTACCTTTTGGAGCTGGAGAAGGAAGGGTTCAACGTCATCCCTACCGCACGCGCCGCGAA
CCTCACCATGAACCGCGAGGGGATCCGCAGGCTCGCCGCCGAGGAACTCGGGCTTCCCACCGCCGCCTACCGTTTCGCGA
CCAGCATGGACGAGTTCCGCGAAGCTGTCGCCGCCATCGGTCTTCCCTGCGTGGTGAAGCCGATCATGAGCTCCTCGGGC
AAGGGGCAGAGCGTGCTGCGCGACGCGGCGGACCTGGAGCGCTGCTTCAAATACGCCATGGAGGGGGCGCGCGGCGCCTC
CGACAAGGTGATCGTGGAACAGTTCATCCCGTTCGACTATGAGATCACGCTCCTCACCGTCCGGCACGCAGGGGGCACCA
GCTTCTGTCCCCCCATCGGGCACCGCCAAATCGACGGCGACTACCACGAGTCGTGGCAGCCGACACCGATGGTTCCTGCG
GTGCTGGCCGAAGCGCAGCGCCAGGCCGAGGCAGTCACCGGCGCTCTCGGCGGGCGCGGCATCTTCGGCGTCGAGTTCTT
CGTCACCGGCGACAAGGTCTGGTTCTCCGAGGTCTCTCCCAGGCCGCACGACACCGGGATGGTCACCATGATCTCCCAGA
ACCTCTCCGAGTTCGAGCTGCACGTGCGCGCTATCCTGGGGCTGCCGGTGCCGCAGGTCGAGAGCCTCGGCTGCGCCGCT
TCGCACGTCATCCTGTCGGAGGGGCAGGCCGCGGAGGTCGCCTTCGAGGGAGTGGCAAAAGCCCTGGAGATCCCCGGCAG
CAAGCTGCGCCTTTTCGGCAAACCCGACACCAGGAAGGGGCGCCGCATGGGCGTGGCGCTCGCCTTCGGCGCCGACTGCG
ACGAGGCCCGCCAGAAGGCCGAACAATCCGCCCACTGTGTAGGCATCGTAAAACGCTAG

Upstream 100 bases:

>100_bases
AAGCGGATCACCGTCCGCGCCAAGCCCGGTTTTCATCAGGAGCAGTTCGAGGTTGCGGTCAGCTAGGACCGCTTGAGAAA
TTAAAGAACCAGGAGAAGTG

Downstream 100 bases:

>100_bases
ACCCGCTGGGATGATCGAAGCCCTTTAATCGCACGCATCTTAGGAGGTTCCATGGTCTTTGCCGCGAAGGTGTCCGAGGT
TCCGGAATTTGGCAAGAAGC

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 392; Mature: 392

Protein sequence:

>392_residues
MIGTPLKNSATRMMLLGSGELGKEVALEAQRLGIEVIAVDRYADAPAMQVAHRSHVIDMLDREQLDRVVRLENPSLIVPE
IEAINTVYLLELEKEGFNVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFATSMDEFREAVAAIGLPCVVKPIMSSSG
KGQSVLRDAADLERCFKYAMEGARGASDKVIVEQFIPFDYEITLLTVRHAGGTSFCPPIGHRQIDGDYHESWQPTPMVPA
VLAEAQRQAEAVTGALGGRGIFGVEFFVTGDKVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPQVESLGCAA
SHVILSEGQAAEVAFEGVAKALEIPGSKLRLFGKPDTRKGRRMGVALAFGADCDEARQKAEQSAHCVGIVKR

Sequences:

>Translated_392_residues
MIGTPLKNSATRMMLLGSGELGKEVALEAQRLGIEVIAVDRYADAPAMQVAHRSHVIDMLDREQLDRVVRLENPSLIVPE
IEAINTVYLLELEKEGFNVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFATSMDEFREAVAAIGLPCVVKPIMSSSG
KGQSVLRDAADLERCFKYAMEGARGASDKVIVEQFIPFDYEITLLTVRHAGGTSFCPPIGHRQIDGDYHESWQPTPMVPA
VLAEAQRQAEAVTGALGGRGIFGVEFFVTGDKVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPQVESLGCAA
SHVILSEGQAAEVAFEGVAKALEIPGSKLRLFGKPDTRKGRRMGVALAFGADCDEARQKAEQSAHCVGIVKR
>Mature_392_residues
MIGTPLKNSATRMMLLGSGELGKEVALEAQRLGIEVIAVDRYADAPAMQVAHRSHVIDMLDREQLDRVVRLENPSLIVPE
IEAINTVYLLELEKEGFNVIPTARAANLTMNREGIRRLAAEELGLPTAAYRFATSMDEFREAVAAIGLPCVVKPIMSSSG
KGQSVLRDAADLERCFKYAMEGARGASDKVIVEQFIPFDYEITLLTVRHAGGTSFCPPIGHRQIDGDYHESWQPTPMVPA
VLAEAQRQAEAVTGALGGRGIFGVEFFVTGDKVWFSEVSPRPHDTGMVTMISQNLSEFELHVRAILGLPVPQVESLGCAA
SHVILSEGQAAEVAFEGVAKALEIPGSKLRLFGKPDTRKGRRMGVALAFGADCDEARQKAEQSAHCVGIVKR

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=391, Percent_Identity=61.8925831202046, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1786733, Length=250, Percent_Identity=27.6, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6324702, Length=380, Percent_Identity=24.7368421052632, Blast_Score=106, Evalue=6e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42548; Mature: 42548

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPLKNSATRMMLLGSGELGKEVALEAQRLGIEVIAVDRYADAPAMQVAHRSHVIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
DREQLDRVVRLENPSLIVPEIEAINTVYLLELEKEGFNVIPTARAANLTMNREGIRRLAA
HHHHHHHHEEECCCCEECCCHHHCCEEEEEEEECCCCCEECCCCCCCEEECHHHHHHHHH
EELGLPTAAYRFATSMDEFREAVAAIGLPCVVKPIMSSSGKGQSVLRDAADLERCFKYAM
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGARGASDKVIVEQFIPFDYEITLLTVRHAGGTSFCPPIGHRQIDGDYHESWQPTPMVPA
HCCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCHHH
VLAEAQRQAEAVTGALGGRGIFGVEFFVTGDKVWFSEVSPRPHDTGMVTMISQNLSEFEL
HHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRAILGLPVPQVESLGCAASHVILSEGQAAEVAFEGVAKALEIPGSKLRLFGKPDTRKG
HHHHHHCCCCCCHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
RRMGVALAFGADCDEARQKAEQSAHCVGIVKR
CEEEEEEEECCCHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MIGTPLKNSATRMMLLGSGELGKEVALEAQRLGIEVIAVDRYADAPAMQVAHRSHVIDML
CCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHHHH
DREQLDRVVRLENPSLIVPEIEAINTVYLLELEKEGFNVIPTARAANLTMNREGIRRLAA
HHHHHHHHEEECCCCEECCCHHHCCEEEEEEEECCCCCEECCCCCCCEEECHHHHHHHHH
EELGLPTAAYRFATSMDEFREAVAAIGLPCVVKPIMSSSGKGQSVLRDAADLERCFKYAM
HHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EGARGASDKVIVEQFIPFDYEITLLTVRHAGGTSFCPPIGHRQIDGDYHESWQPTPMVPA
HCCCCCCCCCHHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHCCCCCCCCHHH
VLAEAQRQAEAVTGALGGRGIFGVEFFVTGDKVWFSEVSPRPHDTGMVTMISQNLSEFEL
HHHHHHHHHHHHHHCCCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRAILGLPVPQVESLGCAASHVILSEGQAAEVAFEGVAKALEIPGSKLRLFGKPDTRKG
HHHHHHCCCCCCHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCC
RRMGVALAFGADCDEARQKAEQSAHCVGIVKR
CEEEEEEEECCCHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA