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The map label for this gene is cafA [H]

Identifier: 197116559

GI number: 197116559

Start: 190285

End: 191775

Strand: Direct

Name: cafA [H]

Synonym: Gbem_0159

Alternate gene names: 197116559

Gene position: 190285-191775 (Clockwise)

Preceding gene: 197116558

Following gene: 197116560

Centisome position: 4.12

GC content: 60.83

Gene sequence:

>1491_bases
ATGGGAAACGAGCTGGTCATAAACACCACCTCCCACGAAACCCGCATCGCGCTCATCGAAAACGGCACCATTGCGGAGCT
GTACGTCGAGAGGAGCAGGGTGAAGGGGATCATCGGCAACATCTACAAGGGAAGAGTGGTCCGGGTGCTGCCGGGCATGC
AGGCGGCGTTCGTGGACATCGGGCTGGAAAAGGCAGCCTTTCTTTACGTGGCGGACGTCTTCGACGCCATGGACGAGTAC
GACAGCTACATCGAGGGTGAGCAGGGCGACGAGCCGATGCCGCACCCGCTGCACCCCATCGAGGAACTGCTGCAGGAGGG
GCAGGAACTCCTGGTGCAGATCTCCAAGGAGCCTATCGGGACCAAGGGGGCTCGCATCACGGCGCACATCTCGCTTCCTG
GGCGCCACCTGGTTTACATGCCGACGGTCGACCACGTCGGCATCTCCAGGAGGATCGAGGACGAAGCGGAGCGCGAGCGT
CTGAAGGAGATCGTGGACCGCATCAAGCCGGTCGGCGGCGGGTTCATCGTCAGGACAGTCTCCGACGGCAAGAGCGAAGA
GGACCTGGTGGCGGACCTCCACTACCTGACCAAGCTCTGGGACGAGATCGCCAAGAAGAAGGACAAGGCGGGAGCCCCGA
CCCTGATCCACTCCGATCTCGACGTAACCCAGAAGGTGGTGCGCGACATCCTCACCGAATCGGTGGAGCGGATCGTAGTG
GACTCGAAGCCCGAGTACGACAAGATCGTCCAGTTCATCAGCACCTTCATGCCCAAGATGAAGTACTCCATCGAGCTTTA
CGACGAAGAGGAGCCGATCTTCGACCATTTCGGGCTGGAGGTCGAGATCAGCCGCGCCTTGGGGCGCAAGGTCTGGCTCA
AGAGCGGCGGCTACATAATCATCGAGCAGACCGAGGCGTTGACCGCGATAGACGTCAACACCGGGCGCTACGTCGGCAAG
CACAACCTGGAAGACACCATCCTGAAGACCAACCTGGAGGCGGTGAAGGAGATCGCCTACCAGCTGCGCCTCAGGAACCT
GGGCGGCATCATCATTATCGACTTCATCGACATGGAGAAGGAGGTGAACCGCGAGAAGGTGTACGGCGCGCTAGAAGAGG
CACTGAAGAGCGACAAGTCCAAGACCAACATCCTGAAGATCTCGGAGCTGGGGCTCGTGGAGATGACCCGCAAGAGGGTG
CGCGAGAGCCTGGGACGCATGATGTGCGAGCCCTGCCCCTACTGCGAGGGGCGCGGTTACGTGAAGTCCAAGATCTCGGT
CTGCCACGAGATCTTCCGTGAGCTGCGTCGCGAGATGCTGGACATCCGCGGCACCAAGGTGATGCTCACCGTGCATCCCC
AGGTGGCCGACCTACTTTACGACGAGGAGCGGCGCGGGCTGGAGGAATTGGAGAAGAACTTCAAGAAGCGGATCACCGTC
CGCGCCAAGCCCGGTTTTCATCAGGAGCAGTTCGAGGTTGCGGTCAGCTAG

Upstream 100 bases:

>100_bases
TGGCGGATTGAGCAGGAATGAACTCGAACCTTGAACGTTGAACCTTGAACAGTTTTTAAGTTTTTGAGTTTTTGAATTTC
ATATATAGGAAGGGGTTAGC

Downstream 100 bases:

>100_bases
GACCGCTTGAGAAATTAAAGAACCAGGAGAAGTGATGATAGGAACGCCGCTGAAAAACAGTGCGACGAGGATGATGCTGC
TGGGCTCAGGCGAGCTAGGC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MGNELVINTTSHETRIALIENGTIAELYVERSRVKGIIGNIYKGRVVRVLPGMQAAFVDIGLEKAAFLYVADVFDAMDEY
DSYIEGEQGDEPMPHPLHPIEELLQEGQELLVQISKEPIGTKGARITAHISLPGRHLVYMPTVDHVGISRRIEDEAERER
LKEIVDRIKPVGGGFIVRTVSDGKSEEDLVADLHYLTKLWDEIAKKKDKAGAPTLIHSDLDVTQKVVRDILTESVERIVV
DSKPEYDKIVQFISTFMPKMKYSIELYDEEEPIFDHFGLEVEISRALGRKVWLKSGGYIIIEQTEALTAIDVNTGRYVGK
HNLEDTILKTNLEAVKEIAYQLRLRNLGGIIIIDFIDMEKEVNREKVYGALEEALKSDKSKTNILKISELGLVEMTRKRV
RESLGRMMCEPCPYCEGRGYVKSKISVCHEIFRELRREMLDIRGTKVMLTVHPQVADLLYDEERRGLEELEKNFKKRITV
RAKPGFHQEQFEVAVS

Sequences:

>Translated_496_residues
MGNELVINTTSHETRIALIENGTIAELYVERSRVKGIIGNIYKGRVVRVLPGMQAAFVDIGLEKAAFLYVADVFDAMDEY
DSYIEGEQGDEPMPHPLHPIEELLQEGQELLVQISKEPIGTKGARITAHISLPGRHLVYMPTVDHVGISRRIEDEAERER
LKEIVDRIKPVGGGFIVRTVSDGKSEEDLVADLHYLTKLWDEIAKKKDKAGAPTLIHSDLDVTQKVVRDILTESVERIVV
DSKPEYDKIVQFISTFMPKMKYSIELYDEEEPIFDHFGLEVEISRALGRKVWLKSGGYIIIEQTEALTAIDVNTGRYVGK
HNLEDTILKTNLEAVKEIAYQLRLRNLGGIIIIDFIDMEKEVNREKVYGALEEALKSDKSKTNILKISELGLVEMTRKRV
RESLGRMMCEPCPYCEGRGYVKSKISVCHEIFRELRREMLDIRGTKVMLTVHPQVADLLYDEERRGLEELEKNFKKRITV
RAKPGFHQEQFEVAVS
>Mature_495_residues
GNELVINTTSHETRIALIENGTIAELYVERSRVKGIIGNIYKGRVVRVLPGMQAAFVDIGLEKAAFLYVADVFDAMDEYD
SYIEGEQGDEPMPHPLHPIEELLQEGQELLVQISKEPIGTKGARITAHISLPGRHLVYMPTVDHVGISRRIEDEAERERL
KEIVDRIKPVGGGFIVRTVSDGKSEEDLVADLHYLTKLWDEIAKKKDKAGAPTLIHSDLDVTQKVVRDILTESVERIVVD
SKPEYDKIVQFISTFMPKMKYSIELYDEEEPIFDHFGLEVEISRALGRKVWLKSGGYIIIEQTEALTAIDVNTGRYVGKH
NLEDTILKTNLEAVKEIAYQLRLRNLGGIIIIDFIDMEKEVNREKVYGALEEALKSDKSKTNILKISELGLVEMTRKRVR
ESLGRMMCEPCPYCEGRGYVKSKISVCHEIFRELRREMLDIRGTKVMLTVHPQVADLLYDEERRGLEELEKNFKKRITVR
AKPGFHQEQFEVAVS

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=495, Percent_Identity=48.0808080808081, Blast_Score=453, Evalue=1e-128,
Organism=Escherichia coli, GI1787325, Length=471, Percent_Identity=38.6411889596603, Blast_Score=296, Evalue=3e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 56536; Mature: 56405

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGNELVINTTSHETRIALIENGTIAELYVERSRVKGIIGNIYKGRVVRVLPGMQAAFVDI
CCCEEEEEECCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
GLEKAAFLYVADVFDAMDEYDSYIEGEQGDEPMPHPLHPIEELLQEGQELLVQISKEPIG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
TKGARITAHISLPGRHLVYMPTVDHVGISRRIEDEAERERLKEIVDRIKPVGGGFIVRTV
CCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SDGKSEEDLVADLHYLTKLWDEIAKKKDKAGAPTLIHSDLDVTQKVVRDILTESVERIVV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHC
DSKPEYDKIVQFISTFMPKMKYSIELYDEEEPIFDHFGLEVEISRALGRKVWLKSGGYII
CCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHCCCEEEEHHHHCCEEEEECCCEEE
IEQTEALTAIDVNTGRYVGKHNLEDTILKTNLEAVKEIAYQLRLRNLGGIIIIDFIDMEK
EEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHH
EVNREKVYGALEEALKSDKSKTNILKISELGLVEMTRKRVRESLGRMMCEPCPYCEGRGY
HCCHHHHHHHHHHHHHCCCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
VKSKISVCHEIFRELRREMLDIRGTKVMLTVHPQVADLLYDEERRGLEELEKNFKKRITV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCEEE
RAKPGFHQEQFEVAVS
EECCCCCCCHHEEECC
>Mature Secondary Structure 
GNELVINTTSHETRIALIENGTIAELYVERSRVKGIIGNIYKGRVVRVLPGMQAAFVDI
CCEEEEEECCCCEEEEEEECCCEEHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHH
GLEKAAFLYVADVFDAMDEYDSYIEGEQGDEPMPHPLHPIEELLQEGQELLVQISKEPIG
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
TKGARITAHISLPGRHLVYMPTVDHVGISRRIEDEAERERLKEIVDRIKPVGGGFIVRTV
CCCCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
SDGKSEEDLVADLHYLTKLWDEIAKKKDKAGAPTLIHSDLDVTQKVVRDILTESVERIVV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHC
DSKPEYDKIVQFISTFMPKMKYSIELYDEEEPIFDHFGLEVEISRALGRKVWLKSGGYII
CCCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCHHHCCCEEEEHHHHCCEEEEECCCEEE
IEQTEALTAIDVNTGRYVGKHNLEDTILKTNLEAVKEIAYQLRLRNLGGIIIIDFIDMEK
EEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHH
EVNREKVYGALEEALKSDKSKTNILKISELGLVEMTRKRVRESLGRMMCEPCPYCEGRGY
HCCHHHHHHHHHHHHHCCCCCCCEEEEHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCH
VKSKISVCHEIFRELRREMLDIRGTKVMLTVHPQVADLLYDEERRGLEELEKNFKKRITV
HHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHCEEE
RAKPGFHQEQFEVAVS
EECCCCCCCHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]