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The map label for this gene is xpsE [H]

Identifier: 197116483

GI number: 197116483

Start: 101019

End: 102677

Strand: Direct

Name: xpsE [H]

Synonym: Gbem_0083

Alternate gene names: 197116483

Gene position: 101019-102677 (Clockwise)

Preceding gene: 197116481

Following gene: 197116484

Centisome position: 2.19

GC content: 64.26

Gene sequence:

>1659_bases
ATGAACGGGCTTGTCAAAGAAGGATCCATCGGCGAGATCCTTTTCAAATCGCAGATCATCACGGAGCAGGAACTGAGCGC
GGCGCTCGAAGAGCAGAAGGTCTCGGGGTGCCGGGTGGGCGAGGCGCTGGTCCGTCTGGGGGTGGTTGCCCAGGAGGATA
TCGACTGGGCGCTCGCCAACCAGCTGAACATCCCCTATGTGCGGCTCAAGAAGGAAAACATCGATCCCGCCGCGGTGGCG
AAGGTCCCGGGACAACTGGCCCGGCGCTACAGCCTCTGCCCCATCTTCCTTTCCGGCAGCGAACTCTCCATCGCCATGGC
GGACCCCCTGAACAAGGAGGCGGTCGAAGAGATCACCCGGGTGACCGGCTGCCAGATCAGCATCTCGGTGGGGCTCATCC
GCGAGATCCGCGAGATGCACGACGCCATGTACGGCCCGGACCAGAACCTCCCGGAACTGGGATTCAGCTCCGGGCACTTC
CCCGCCAAGGTCCTCTCCGCCATCAACGCCGACCTTTCCGGCGCCATGCTCCTCAACCACCTTCTCTTGCGCGTGGTACA
GAAGAAGTTCGCCTCGGTAGCACTCCAGCCGCTGGGGGACCAGGTGCGGGTGCTGGCGCGCGGCGAAGGTAGGACGTCCG
AGTTAGGCAAGCTCTCCGCGACCCACTACGGGCGGCTCACCGAGCGCATCCGCCGCCTCTCAGGCATCGGCGGCGCCGAG
GAGAACCCCTCCAGCGGCATCTTGACCTTCATCTGGCAGGGGAAAAGGATCCCGTTCCAGACCCTGGTGATGCCCGGCAA
CGGAGGGGATTACCTCACCTTGAAGCTCCACGTCGGCGCGCCGAAAATCTCGGAGCTGGACGACCTCGGTGTCTCCGCCG
GGAAACGCGCGGACCTGAAGGCGCTCTGCTCGGAAAAGGAGGGGCTGGTCCTCTTCACCGGGCGCGACCCGGAAGAGCGG
AGCCGGCTCATCGACCTGTTCCTGGATGCCTGCGATCACGCCGACCGCACCGTCATTTTGGTGGGGGAAAGGCTTGGTCG
CGGCAGGGACCGCTGGCCGCGGCTGCCGGCGGGAAGATGCGGCGCGGACGATACCGCAAAGGTGGTGTCGGCGGCCCTGG
AGCATGACCCGGACACCCTGGTGATCGAGGACGTCACGGAACTTGCCTCCTTCATAGCGGCGAGCAAGGCGGTGATGCGG
GGGAAGCTGGTGGTGGCGGGGATGTCCCAGGGGAACAAGGGGGCGGTGTTGAAGCAGCTCCTGTACCTCTCCCAGAAGAA
CTTCCTGATACCGACCCACCTGAAAGGGGTGGTTTCCTTGAAGTGCGTGCTGCTCCTTTGCCCGGAGTGCAAGAAGCAGT
ACACGCCTGCCGCAGACGAGCTGGCGGCCTTGCGGCTTAAGGCGACGGCGCCGGAGTATTTCCGCCCGACCGGCTGTCCC
TCCTGCGACCAGACAGGCTATAGCGGTAAGAAATACCTCCTGGACGTGATCCGGTTCGACCAGGGGCTCCTGGAGGCGTT
CGAGGTGATCCGCGATTCCGACGAGATCATCCGCCACCTCAAAGACAACGGCTACCGGGGCATCGGCGAAGAAGGGGCCG
AGCTCCTGGAGCGGGGAGAAATATCCCCGGGCGAGTACGTCGCTTCCATACTACTGTAA

Upstream 100 bases:

>100_bases
TTGCTTTTGAGCTTGTCCGTTGTCAATTGTCAATTGTTTTAGAAAGTGTTTTCAGGTACATTTCCCAGGCACCGCCATCT
GCAGCATCTGGAGGTAGGCA

Downstream 100 bases:

>100_bases
CGGAGAAGAGACATGGCAAGGATTGACGCACTGTTCAAGCTGTTGCACGAGGCCGGGGCCTCCGACCTGCACCTTTCCGC
CGGATCCCCGCCGATCTTCC

Product: PilB/PulE/GspE family ATPase

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 552; Mature: 552

Protein sequence:

>552_residues
MNGLVKEGSIGEILFKSQIITEQELSAALEEQKVSGCRVGEALVRLGVVAQEDIDWALANQLNIPYVRLKKENIDPAAVA
KVPGQLARRYSLCPIFLSGSELSIAMADPLNKEAVEEITRVTGCQISISVGLIREIREMHDAMYGPDQNLPELGFSSGHF
PAKVLSAINADLSGAMLLNHLLLRVVQKKFASVALQPLGDQVRVLARGEGRTSELGKLSATHYGRLTERIRRLSGIGGAE
ENPSSGILTFIWQGKRIPFQTLVMPGNGGDYLTLKLHVGAPKISELDDLGVSAGKRADLKALCSEKEGLVLFTGRDPEER
SRLIDLFLDACDHADRTVILVGERLGRGRDRWPRLPAGRCGADDTAKVVSAALEHDPDTLVIEDVTELASFIAASKAVMR
GKLVVAGMSQGNKGAVLKQLLYLSQKNFLIPTHLKGVVSLKCVLLLCPECKKQYTPAADELAALRLKATAPEYFRPTGCP
SCDQTGYSGKKYLLDVIRFDQGLLEAFEVIRDSDEIIRHLKDNGYRGIGEEGAELLERGEISPGEYVASILL

Sequences:

>Translated_552_residues
MNGLVKEGSIGEILFKSQIITEQELSAALEEQKVSGCRVGEALVRLGVVAQEDIDWALANQLNIPYVRLKKENIDPAAVA
KVPGQLARRYSLCPIFLSGSELSIAMADPLNKEAVEEITRVTGCQISISVGLIREIREMHDAMYGPDQNLPELGFSSGHF
PAKVLSAINADLSGAMLLNHLLLRVVQKKFASVALQPLGDQVRVLARGEGRTSELGKLSATHYGRLTERIRRLSGIGGAE
ENPSSGILTFIWQGKRIPFQTLVMPGNGGDYLTLKLHVGAPKISELDDLGVSAGKRADLKALCSEKEGLVLFTGRDPEER
SRLIDLFLDACDHADRTVILVGERLGRGRDRWPRLPAGRCGADDTAKVVSAALEHDPDTLVIEDVTELASFIAASKAVMR
GKLVVAGMSQGNKGAVLKQLLYLSQKNFLIPTHLKGVVSLKCVLLLCPECKKQYTPAADELAALRLKATAPEYFRPTGCP
SCDQTGYSGKKYLLDVIRFDQGLLEAFEVIRDSDEIIRHLKDNGYRGIGEEGAELLERGEISPGEYVASILL
>Mature_552_residues
MNGLVKEGSIGEILFKSQIITEQELSAALEEQKVSGCRVGEALVRLGVVAQEDIDWALANQLNIPYVRLKKENIDPAAVA
KVPGQLARRYSLCPIFLSGSELSIAMADPLNKEAVEEITRVTGCQISISVGLIREIREMHDAMYGPDQNLPELGFSSGHF
PAKVLSAINADLSGAMLLNHLLLRVVQKKFASVALQPLGDQVRVLARGEGRTSELGKLSATHYGRLTERIRRLSGIGGAE
ENPSSGILTFIWQGKRIPFQTLVMPGNGGDYLTLKLHVGAPKISELDDLGVSAGKRADLKALCSEKEGLVLFTGRDPEER
SRLIDLFLDACDHADRTVILVGERLGRGRDRWPRLPAGRCGADDTAKVVSAALEHDPDTLVIEDVTELASFIAASKAVMR
GKLVVAGMSQGNKGAVLKQLLYLSQKNFLIPTHLKGVVSLKCVLLLCPECKKQYTPAADELAALRLKATAPEYFRPTGCP
SCDQTGYSGKKYLLDVIRFDQGLLEAFEVIRDSDEIIRHLKDNGYRGIGEEGAELLERGEISPGEYVASILL

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=319, Percent_Identity=21.6300940438871, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1786296, Length=453, Percent_Identity=22.7373068432671, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786781, Length=123, Percent_Identity=27.6422764227642, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007831
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 60028; Mature: 60028

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGLVKEGSIGEILFKSQIITEQELSAALEEQKVSGCRVGEALVRLGVVAQEDIDWALAN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCHHHHC
QLNIPYVRLKKENIDPAAVAKVPGQLARRYSLCPIFLSGSELSIAMADPLNKEAVEEITR
CCCCCEEEEECCCCCHHHHHHCCHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHH
VTGCQISISVGLIREIREMHDAMYGPDQNLPELGFSSGHFPAKVLSAINADLSGAMLLNH
HCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LLLRVVQKKFASVALQPLGDQVRVLARGEGRTSELGKLSATHYGRLTERIRRLSGIGGAE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ENPSSGILTFIWQGKRIPFQTLVMPGNGGDYLTLKLHVGAPKISELDDLGVSAGKRADLK
CCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCCCCHHHH
ALCSEKEGLVLFTGRDPEERSRLIDLFLDACDHADRTVILVGERLGRGRDRWPRLPAGRC
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHCCCCCCCCCCCCCCC
GADDTAKVVSAALEHDPDTLVIEDVTELASFIAASKAVMRGKLVVAGMSQGNKGAVLKQL
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHH
LYLSQKNFLIPTHLKGVVSLKCVLLLCPECKKQYTPAADELAALRLKATAPEYFRPTGCP
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHEECCCCHHCCCCCCC
SCDQTGYSGKKYLLDVIRFDQGLLEAFEVIRDSDEIIRHLKDNGYRGIGEEGAELLERGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
ISPGEYVASILL
CCHHHHHHHHCC
>Mature Secondary Structure
MNGLVKEGSIGEILFKSQIITEQELSAALEEQKVSGCRVGEALVRLGVVAQEDIDWALAN
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHCCCCHHHHC
QLNIPYVRLKKENIDPAAVAKVPGQLARRYSLCPIFLSGSELSIAMADPLNKEAVEEITR
CCCCCEEEEECCCCCHHHHHHCCHHHHHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHH
VTGCQISISVGLIREIREMHDAMYGPDQNLPELGFSSGHFPAKVLSAINADLSGAMLLNH
HCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHH
LLLRVVQKKFASVALQPLGDQVRVLARGEGRTSELGKLSATHYGRLTERIRRLSGIGGAE
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCC
ENPSSGILTFIWQGKRIPFQTLVMPGNGGDYLTLKLHVGAPKISELDDLGVSAGKRADLK
CCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEEECCCCCHHHHHCCCCCCCCHHHH
ALCSEKEGLVLFTGRDPEERSRLIDLFLDACDHADRTVILVGERLGRGRDRWPRLPAGRC
HHHCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEEECHHCCCCCCCCCCCCCCC
GADDTAKVVSAALEHDPDTLVIEDVTELASFIAASKAVMRGKLVVAGMSQGNKGAVLKQL
CCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCHHHHHHH
LYLSQKNFLIPTHLKGVVSLKCVLLLCPECKKQYTPAADELAALRLKATAPEYFRPTGCP
HHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHCCCCCHHHHHHHHEECCCCHHCCCCCCC
SCDQTGYSGKKYLLDVIRFDQGLLEAFEVIRDSDEIIRHLKDNGYRGIGEEGAELLERGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHHHHCC
ISPGEYVASILL
CCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1944223; 12024217 [H]