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The map label for this gene is pilT-3 [H]

Identifier: 197116484

GI number: 197116484

Start: 102690

End: 103763

Strand: Direct

Name: pilT-3 [H]

Synonym: Gbem_0084

Alternate gene names: 197116484

Gene position: 102690-103763 (Clockwise)

Preceding gene: 197116483

Following gene: 197116485

Centisome position: 2.23

GC content: 63.04

Gene sequence:

>1074_bases
ATGGCAAGGATTGACGCACTGTTCAAGCTGTTGCACGAGGCCGGGGCCTCCGACCTGCACCTTTCCGCCGGATCCCCGCC
GATCTTCCGTTTGCGCGGCGAGATGGAGCGGCAGAACTTCAAGTCGCTCGGGCACGAGGAGCTGAAGGCGCTCCTTTACG
AGATCCTGACCCCGAAGCAGCGCGAGACCTTCGAGGAGAAGCACGACCTCGACTTCGCCTACTCGGTACCGGGCCTGGCG
CGCTTCCGCGGCAACTTCATGATGCAGCACCGTGGGATCGCGGCGGTGTTCCGCATCATTCCGACCAAGATACTTTCTGC
CGACGAGCTGGGGCTTCCGGAAGGGATCCGCAATCTGACCAAGCTCAGGAAGGGGCTGGTGCTGGTCACCGGTCCCACGG
GGAGCGGCAAGTCCACGACGCTCGCCGCCATGATAGACCTGATCAACATCACCAGGAGGGAGCACATCCTGACGCTGGAG
GACCCGCTGGAGTTCATCCACGAAAACAAGATGTCCCTTTTCAACCAGCGCCAGATTGGCGAGCACTCCGAGAGCTTCGC
CAGCGCGCTGAGGGCGGCGCTAAGGGAAGACCCGGACGTGATCCTGGTGGGCGAGATGCGGGACCTCGAGACCATCGCCC
TCGCCATGAGCGCCGCTGAGACGGGGCACCTGGTGTTCGGCACCCTGCACACCAGTTCCGCCGCGAAGACCGTGGACAGG
ATCATCGACGTCTTCCCCAAGGACGGGCAGGAGCAGGTGCGCGCCATCCTTTCTGAGTCGCTCCGGGGGGTGGTCTGCCA
GCAGCTGTTGAAGACCGCCGACGGCAAGGGGAGGGCGGCCGCGCAGGAGATCATGGTCTGGAACGCCGCCATCGGGAACC
TGATCCGGGAAGGTAAGACCTTCCAGATCCCCTCCATCATGCAGACCGGCAGAAAGGATGGGATGCAGCTCATGGACCAG
CACATCCTCGACCTGTTGAAGACCAAGAAAATCGCACCCGAGGAGGCGTACCGCTGCTGCACTGACAAGAGGCAGTTCGA
GCAGTACCTCCCGAACAAGCCGGCGGAGCAGTAG

Upstream 100 bases:

>100_bases
CTACCGGGGCATCGGCGAAGAAGGGGCCGAGCTCCTGGAGCGGGGAGAAATATCCCCGGGCGAGTACGTCGCTTCCATAC
TACTGTAACGGAGAAGAGAC

Downstream 100 bases:

>100_bases
CCGGTGCGTTTCCCCGTTTCGCCGCTGATCGCGGGCGTGCATCCGCCTCCCATCTCGGAGGTGAAGAGTTGGATCGCCGG
CGCCCCCGCCGGGCTTCCCC

Product: twitching motility pilus retraction protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MARIDALFKLLHEAGASDLHLSAGSPPIFRLRGEMERQNFKSLGHEELKALLYEILTPKQRETFEEKHDLDFAYSVPGLA
RFRGNFMMQHRGIAAVFRIIPTKILSADELGLPEGIRNLTKLRKGLVLVTGPTGSGKSTTLAAMIDLINITRREHILTLE
DPLEFIHENKMSLFNQRQIGEHSESFASALRAALREDPDVILVGEMRDLETIALAMSAAETGHLVFGTLHTSSAAKTVDR
IIDVFPKDGQEQVRAILSESLRGVVCQQLLKTADGKGRAAAQEIMVWNAAIGNLIREGKTFQIPSIMQTGRKDGMQLMDQ
HILDLLKTKKIAPEEAYRCCTDKRQFEQYLPNKPAEQ

Sequences:

>Translated_357_residues
MARIDALFKLLHEAGASDLHLSAGSPPIFRLRGEMERQNFKSLGHEELKALLYEILTPKQRETFEEKHDLDFAYSVPGLA
RFRGNFMMQHRGIAAVFRIIPTKILSADELGLPEGIRNLTKLRKGLVLVTGPTGSGKSTTLAAMIDLINITRREHILTLE
DPLEFIHENKMSLFNQRQIGEHSESFASALRAALREDPDVILVGEMRDLETIALAMSAAETGHLVFGTLHTSSAAKTVDR
IIDVFPKDGQEQVRAILSESLRGVVCQQLLKTADGKGRAAAQEIMVWNAAIGNLIREGKTFQIPSIMQTGRKDGMQLMDQ
HILDLLKTKKIAPEEAYRCCTDKRQFEQYLPNKPAEQ
>Mature_356_residues
ARIDALFKLLHEAGASDLHLSAGSPPIFRLRGEMERQNFKSLGHEELKALLYEILTPKQRETFEEKHDLDFAYSVPGLAR
FRGNFMMQHRGIAAVFRIIPTKILSADELGLPEGIRNLTKLRKGLVLVTGPTGSGKSTTLAAMIDLINITRREHILTLED
PLEFIHENKMSLFNQRQIGEHSESFASALRAALREDPDVILVGEMRDLETIALAMSAAETGHLVFGTLHTSSAAKTVDRI
IDVFPKDGQEQVRAILSESLRGVVCQQLLKTADGKGRAAAQEIMVWNAAIGNLIREGKTFQIPSIMQTGRKDGMQLMDQH
ILDLLKTKKIAPEEAYRCCTDKRQFEQYLPNKPAEQ

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=299, Percent_Identity=49.1638795986622, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789723, Length=172, Percent_Identity=45.3488372093023, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1786296, Length=149, Percent_Identity=43.6241610738255, Blast_Score=107, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 39893; Mature: 39762

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARIDALFKLLHEAGASDLHLSAGSPPIFRLRGEMERQNFKSLGHEELKALLYEILTPKQ
CCHHHHHHHHHHHCCCCCEEECCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCH
RETFEEKHDLDFAYSVPGLARFRGNFMMQHRGIAAVFRIIPTKILSADELGLPEGIRNLT
HHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
KLRKGLVLVTGPTGSGKSTTLAAMIDLINITRREHILTLEDPLEFIHENKMSLFNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHC
EHSESFASALRAALREDPDVILVGEMRDLETIALAMSAAETGHLVFGTLHTSSAAKTVDR
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
IIDVFPKDGQEQVRAILSESLRGVVCQQLLKTADGKGRAAAQEIMVWNAAIGNLIREGKT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
FQIPSIMQTGRKDGMQLMDQHILDLLKTKKIAPEEAYRCCTDKRQFEQYLPNKPAEQ
EECHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARIDALFKLLHEAGASDLHLSAGSPPIFRLRGEMERQNFKSLGHEELKALLYEILTPKQ
CHHHHHHHHHHHCCCCCEEECCCCCCEEECCCHHHHHHHHHCCHHHHHHHHHHHHCCCH
RETFEEKHDLDFAYSVPGLARFRGNFMMQHRGIAAVFRIIPTKILSADELGLPEGIRNLT
HHHHHHHHCCCEEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHH
KLRKGLVLVTGPTGSGKSTTLAAMIDLINITRREHILTLEDPLEFIHENKMSLFNQRQIG
HHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHC
EHSESFASALRAALREDPDVILVGEMRDLETIALAMSAAETGHLVFGTLHTSSAAKTVDR
HHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHH
IIDVFPKDGQEQVRAILSESLRGVVCQQLLKTADGKGRAAAQEIMVWNAAIGNLIREGKT
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
FQIPSIMQTGRKDGMQLMDQHILDLLKTKKIAPEEAYRCCTDKRQFEQYLPNKPAEQ
EECHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]