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The map label for this gene is metZ [H]

Identifier: 197106397

GI number: 197106397

Start: 3358255

End: 3359439

Strand: Direct

Name: metZ [H]

Synonym: PHZ_c2936

Alternate gene names: 197106397

Gene position: 3358255-3359439 (Clockwise)

Preceding gene: 197106392

Following gene: 197106402

Centisome position: 84.04

GC content: 71.22

Gene sequence:

>1185_bases
ATGGCGGAGGACCCGCAGGATTGGGAGATCGAGACCCAGGCGGTGCGCGGCGGCCTCGCACGCACGCCCTACGGCGAGAT
CTCGGAAGCCCTCTTCCTGACCCAGAGCTTCGCCTACGAGTCGGCCGAGGCGGCCGACCGGCGCTTCTCGGGCGAGGAGC
CCGGCTTCATCTACCAGCGCTTCGGCAACCCCACGACCGACATCTTCGAGCGCCGCCTGGCGCTGATCGAGGGCGCCGAG
GCCTGCCGGGCGACCGCCTCGGGCATGGCGGCCGTGCAGCTTTCCCTGATGGGCCTGGTCCGCGCTGGCGACCACATTGT
CGCGGGCCGGGCCCTGTTCGGCTCGTGCCGCTGGATCCTGTCGGAATGGATGCCGAGGTTCGGGGTCGAGACGACCTATG
TGGACGCCACGGACCTCTCCGCCTGGAAGAACGCGGTGCGGCCGAACACCAGGGCGTTCCTGATCGAGAGCCCGGCGAAC
CCGCTGCTGGAGGTGACCGACATCGCCGGCGTGGCCCAGATCGCCCGGGAGGCCGGCGCGAAACTGGTGGTGGACAACGT
CTTCGCCACGCCGGTCTTCCAGAAGCCGCTGGCGCTGGGCGCCGACGTGGTCGTCTATTCGGCGACCAAGCACATCGACG
GCCAGGGACGGGTGCTGGGCGGGGCGATCCTCGGCTCGGCCGAGCTGATCACCGAGGCCTACAAGGACATGCATCGGCAC
ACGGGCCCGGCCCTGTCGCCGTTCAACGCCTGGGTGCTGCTGAAGGGGCTGGAGACCATGGACCTGCGGGTCCGCCGCCA
GACCGAGACCGCCGCGACGGTGGCCGACGCCGTCGCCGCCCACGCCAAGGCCCGCCAGGTGGTCTACTGCGGCAGGGACG
ACCACCCGCAGGCGGCCGTGATCGCCCGCCAGATGAGCGGCGGCGGCAACGTCATCGCCTTCGACCTCGGCAGCCGCGAG
GCGGCGTGGCGGTTCCTGGACGCGCTGGAGATCGTCGACATCTCCAACAACCTGGGCGACGCCAAGTCGATGGCCACGCA
CCCCTCGACCACCACCCACCGCTCGATGCCCGAAGCCGAGCGCGAGGCCATCGGCCTGACGCAAGGCTGGGTGCGGATGA
GCGTCGGCCTCGAAGGCGCCCGCGACCTCGTGCGCGACGTGACCCGGGCGCTGGACCGGGCCTAG

Upstream 100 bases:

>100_bases
GCAAGGGCGATGGCGCCGCTTGGCCGAAACCGGCCCTTGACCGCCCAGGCGCCGGGGCGTTCCTGCCCTCAGCTTTTTCG
AAGAGGCGCTGGAGGCCGTG

Downstream 100 bases:

>100_bases
GCCGCCGTCTCGAGGGACGCCGGCTCCACGGCGTAGACGCGCGAGCAGTACTCGCAGGTCACGTGGATCTTGCCGTCGGG
CTCGACCATGCCCTCGCGGT

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MAEDPQDWEIETQAVRGGLARTPYGEISEALFLTQSFAYESAEAADRRFSGEEPGFIYQRFGNPTTDIFERRLALIEGAE
ACRATASGMAAVQLSLMGLVRAGDHIVAGRALFGSCRWILSEWMPRFGVETTYVDATDLSAWKNAVRPNTRAFLIESPAN
PLLEVTDIAGVAQIAREAGAKLVVDNVFATPVFQKPLALGADVVVYSATKHIDGQGRVLGGAILGSAELITEAYKDMHRH
TGPALSPFNAWVLLKGLETMDLRVRRQTETAATVADAVAAHAKARQVVYCGRDDHPQAAVIARQMSGGGNVIAFDLGSRE
AAWRFLDALEIVDISNNLGDAKSMATHPSTTTHRSMPEAEREAIGLTQGWVRMSVGLEGARDLVRDVTRALDRA

Sequences:

>Translated_394_residues
MAEDPQDWEIETQAVRGGLARTPYGEISEALFLTQSFAYESAEAADRRFSGEEPGFIYQRFGNPTTDIFERRLALIEGAE
ACRATASGMAAVQLSLMGLVRAGDHIVAGRALFGSCRWILSEWMPRFGVETTYVDATDLSAWKNAVRPNTRAFLIESPAN
PLLEVTDIAGVAQIAREAGAKLVVDNVFATPVFQKPLALGADVVVYSATKHIDGQGRVLGGAILGSAELITEAYKDMHRH
TGPALSPFNAWVLLKGLETMDLRVRRQTETAATVADAVAAHAKARQVVYCGRDDHPQAAVIARQMSGGGNVIAFDLGSRE
AAWRFLDALEIVDISNNLGDAKSMATHPSTTTHRSMPEAEREAIGLTQGWVRMSVGLEGARDLVRDVTRALDRA
>Mature_393_residues
AEDPQDWEIETQAVRGGLARTPYGEISEALFLTQSFAYESAEAADRRFSGEEPGFIYQRFGNPTTDIFERRLALIEGAEA
CRATASGMAAVQLSLMGLVRAGDHIVAGRALFGSCRWILSEWMPRFGVETTYVDATDLSAWKNAVRPNTRAFLIESPANP
LLEVTDIAGVAQIAREAGAKLVVDNVFATPVFQKPLALGADVVVYSATKHIDGQGRVLGGAILGSAELITEAYKDMHRHT
GPALSPFNAWVLLKGLETMDLRVRRQTETAATVADAVAAHAKARQVVYCGRDDHPQAAVIARQMSGGGNVIAFDLGSREA
AWRFLDALEIVDISNNLGDAKSMATHPSTTTHRSMPEAEREAIGLTQGWVRMSVGLEGARDLVRDVTRALDRA

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=328, Percent_Identity=32.9268292682927, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI299473759, Length=328, Percent_Identity=30.4878048780488, Blast_Score=178, Evalue=9e-45,
Organism=Homo sapiens, GI25453487, Length=327, Percent_Identity=26.605504587156, Blast_Score=152, Evalue=4e-37,
Organism=Escherichia coli, GI1790375, Length=380, Percent_Identity=31.0526315789474, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1789383, Length=399, Percent_Identity=23.5588972431078, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560056, Length=332, Percent_Identity=38.2530120481928, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17538055, Length=332, Percent_Identity=38.2530120481928, Blast_Score=239, Evalue=1e-63,
Organism=Caenorhabditis elegans, GI71980708, Length=343, Percent_Identity=32.3615160349854, Blast_Score=200, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319307, Length=393, Percent_Identity=32.3155216284987, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6323333, Length=408, Percent_Identity=28.4313725490196, Blast_Score=175, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=361, Percent_Identity=27.4238227146814, Blast_Score=139, Evalue=9e-34,
Organism=Saccharomyces cerevisiae, GI6321170, Length=268, Percent_Identity=27.6119402985075, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=24.3654822335025, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19922554, Length=347, Percent_Identity=34.5821325648415, Blast_Score=229, Evalue=3e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42640; Mature: 42508

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEDPQDWEIETQAVRGGLARTPYGEISEALFLTQSFAYESAEAADRRFSGEEPGFIYQR
CCCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEE
FGNPTTDIFERRLALIEGAEACRATASGMAAVQLSLMGLVRAGDHIVAGRALFGSCRWIL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
SEWMPRFGVETTYVDATDLSAWKNAVRPNTRAFLIESPANPLLEVTDIAGVAQIAREAGA
HHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCC
KLVVDNVFATPVFQKPLALGADVVVYSATKHIDGQGRVLGGAILGSAELITEAYKDMHRH
EEEEHHHHCCCHHHCHHHCCCCEEEEECHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHHH
TGPALSPFNAWVLLKGLETMDLRVRRQTETAATVADAVAAHAKARQVVYCGRDDHPQAAV
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
IARQMSGGGNVIAFDLGSREAAWRFLDALEIVDISNNLGDAKSMATHPSTTTHRSMPEAE
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHH
REAIGLTQGWVRMSVGLEGARDLVRDVTRALDRA
HHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEDPQDWEIETQAVRGGLARTPYGEISEALFLTQSFAYESAEAADRRFSGEEPGFIYQR
CCCCCCCCEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCEEEEE
FGNPTTDIFERRLALIEGAEACRATASGMAAVQLSLMGLVRAGDHIVAGRALFGSCRWIL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHH
SEWMPRFGVETTYVDATDLSAWKNAVRPNTRAFLIESPANPLLEVTDIAGVAQIAREAGA
HHHHHHHCCCEEEECCHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCC
KLVVDNVFATPVFQKPLALGADVVVYSATKHIDGQGRVLGGAILGSAELITEAYKDMHRH
EEEEHHHHCCCHHHCHHHCCCCEEEEECHHCCCCCCCEEEHHHHCHHHHHHHHHHHHHHH
TGPALSPFNAWVLLKGLETMDLRVRRQTETAATVADAVAAHAKARQVVYCGRDDHPQAAV
CCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHH
IARQMSGGGNVIAFDLGSREAAWRFLDALEIVDISNNLGDAKSMATHPSTTTHRSMPEAE
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHEEECCCCCCCHHHHCCCCCCCCCCCCCHHH
REAIGLTQGWVRMSVGLEGARDLVRDVTRALDRA
HHHCCCCHHHEEEECCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]