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The map label for this gene is ahpF [H]

Identifier: 197106186

GI number: 197106186

Start: 3121234

End: 3122817

Strand: Direct

Name: ahpF [H]

Synonym: PHZ_c2725

Alternate gene names: 197106186

Gene position: 3121234-3122817 (Clockwise)

Preceding gene: 197106185

Following gene: 197106190

Centisome position: 78.1

GC content: 69.32

Gene sequence:

>1584_bases
ATGCTGGACGACAATCTGAAGGCCCAGCTGCGGGCCTATCTGGAGAACATCCGCCAGCCGGTGGAGCTGGTGGCCTCGCT
GGACGACGGCGCCAAGTCCAAGGAGCTGCTCGAGCTCCTGGAAGAGATCGCCGCCGCCTCGGACAAGGTGCGCGTGGTCA
CCGGCGGCGACGACGCCCGCAAGCCCTCGTTCGCCATCGTGCGGGCCGGGACCGACGTTTCCGTGCGCTTCGCGGGCCTC
CCGCTCGGCCACGAGTTCACCTCGCTGGTGCTGACCCTGCTGTGGGTCGGCGGCCATCCGCCCAAGGTGGAGCCCGAGGT
GATCGAACAGGTGAAGGCGCTCGACGGCGACTTCTCGTTCGAGACCTATTTCTCGCTCTCGTGCCAGAACTGCCCCGACG
TGGTGCAGGCGCTGAACCTGATGAGCGTGCTGAACCCGAAGATCCGCCACGTCTCCATCGACGGGGCCCTGTTCCAGGAC
GAAGTCGAGAAGCGCCAGGTCATGGCGGTTCCCACCGTGCTGCTGAACGGCGAGCCGTTCGCGCAAGGGCGCATGGAGCT
CGGCCAGATCCTCGCCAAGATCGACACCGGCGCCCAGGCCCGCGCGGCCGAGAAGATCAAGGGCAAGGACGCCTTCGACG
TGCTGGTGGTGGGCGGCGGCCCGGCCGGCGCGGCCGCGGCCATCTACGCCGCCCGCAAGGGCATCCGCACGGGCCTCGCC
GCCGAACGCTTCGGCGGTCAGGTGCTCGACACCCTGGCGATCGAGAACTTCATCTCGGTGCCGCACACCGAAGGGCCCAA
GCTGGCGGCCGGCCTCGAGCAGCACGTCAAGGACTACGACGTCGACGTGATGAACCTGCAGAAGGCGACCAAGCTGGTCC
CGGCCCGCGAGCCGGGCGGCCTGATCACCGTCGAGCTGGAGAACGGCGCCTCGCTGAAGTCGCGGACCGTCATCCTCGCC
ACCGGCGCCCGCTGGCGGCAGATGAACGTCCCCGGCGAGGACGCCTACCGCAACAAGGGCGTGGCCTACTGCCCGCACTG
CGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAACTCCGGCGTCGAGGCGGCCATCGACCTGG
CCGGCATCGTGGCCCACGTCACCCTGATCGAGTTCGACAGCCAGCTGCGCGCCGACGCCGTGCTGCAGCGCAAGCTGGCC
AGCCTGCCGAACGTGCGCATCGTCACCTCGGCCCTGACCACCGAGGTCAAGGGCGACGGCCAGAAGGTGACCGGCCTCGT
CTACAAGGACCGCAACTCGGACGAGGTGCACAACGTCGACCTGGAGGGGATCTTCGTCCAGATCGGCCTGGTGCCGAACA
CCGAGTGGCTGAAGGACTCGGTGGCGCTCTCGCCGCGCGGCGAGATCGAGACCGACTACCGCGGCCAGACCTCGATCCCG
GGCGTGTTCGCGGCCGGCGATGCGACCACCACGCCCTACAAGCAGATCGTCATCGCCATGGGCGAGGGCTCGAAGGCGGC
GCTGTCGGCCTTCGACTACCTGATCCGCCAGCCGGTCCCGGCGCAGTCGCAGGCCGCGGCCTGA

Upstream 100 bases:

>100_bases
ATCTAAGCCCGACTTCGACGACCCGCCGGCGCCGCGCCTCCTTAGCGCGCGGCGCCGGCGCCATCGCCCTTCCTCCCCCC
CCCCGAGACCCCGGAGTCCC

Downstream 100 bases:

>100_bases
TCCGCCGTCTGCAGGCCGACGCCCTTCGCTAGGTCTCGGCCTCGCCCAGCCTCACCGGACGCACCCCGGTCCGCAGCGCC
GCCCAGGCGCTGTGGACCAG

Product: alkyl hydroperoxide reductase, subunit f

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MLDDNLKAQLRAYLENIRQPVELVASLDDGAKSKELLELLEEIAAASDKVRVVTGGDDARKPSFAIVRAGTDVSVRFAGL
PLGHEFTSLVLTLLWVGGHPPKVEPEVIEQVKALDGDFSFETYFSLSCQNCPDVVQALNLMSVLNPKIRHVSIDGALFQD
EVEKRQVMAVPTVLLNGEPFAQGRMELGQILAKIDTGAQARAAEKIKGKDAFDVLVVGGGPAGAAAAIYAARKGIRTGLA
AERFGGQVLDTLAIENFISVPHTEGPKLAAGLEQHVKDYDVDVMNLQKATKLVPAREPGGLITVELENGASLKSRTVILA
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVRIVTSALTTEVKGDGQKVTGLVYKDRNSDEVHNVDLEGIFVQIGLVPNTEWLKDSVALSPRGEIETDYRGQTSIP
GVFAAGDATTTPYKQIVIAMGEGSKAALSAFDYLIRQPVPAQSQAAA

Sequences:

>Translated_527_residues
MLDDNLKAQLRAYLENIRQPVELVASLDDGAKSKELLELLEEIAAASDKVRVVTGGDDARKPSFAIVRAGTDVSVRFAGL
PLGHEFTSLVLTLLWVGGHPPKVEPEVIEQVKALDGDFSFETYFSLSCQNCPDVVQALNLMSVLNPKIRHVSIDGALFQD
EVEKRQVMAVPTVLLNGEPFAQGRMELGQILAKIDTGAQARAAEKIKGKDAFDVLVVGGGPAGAAAAIYAARKGIRTGLA
AERFGGQVLDTLAIENFISVPHTEGPKLAAGLEQHVKDYDVDVMNLQKATKLVPAREPGGLITVELENGASLKSRTVILA
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVRIVTSALTTEVKGDGQKVTGLVYKDRNSDEVHNVDLEGIFVQIGLVPNTEWLKDSVALSPRGEIETDYRGQTSIP
GVFAAGDATTTPYKQIVIAMGEGSKAALSAFDYLIRQPVPAQSQAAA
>Mature_527_residues
MLDDNLKAQLRAYLENIRQPVELVASLDDGAKSKELLELLEEIAAASDKVRVVTGGDDARKPSFAIVRAGTDVSVRFAGL
PLGHEFTSLVLTLLWVGGHPPKVEPEVIEQVKALDGDFSFETYFSLSCQNCPDVVQALNLMSVLNPKIRHVSIDGALFQD
EVEKRQVMAVPTVLLNGEPFAQGRMELGQILAKIDTGAQARAAEKIKGKDAFDVLVVGGGPAGAAAAIYAARKGIRTGLA
AERFGGQVLDTLAIENFISVPHTEGPKLAAGLEQHVKDYDVDVMNLQKATKLVPAREPGGLITVELENGASLKSRTVILA
TGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVRIVTSALTTEVKGDGQKVTGLVYKDRNSDEVHNVDLEGIFVQIGLVPNTEWLKDSVALSPRGEIETDYRGQTSIP
GVFAAGDATTTPYKQIVIAMGEGSKAALSAFDYLIRQPVPAQSQAAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081763, Length=520, Percent_Identity=65.7692307692308, Blast_Score=689, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=314, Percent_Identity=35.031847133758, Blast_Score=167, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6321898, Length=317, Percent_Identity=33.4384858044164, Blast_Score=165, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6320560, Length=314, Percent_Identity=33.1210191082803, Blast_Score=158, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56243; Mature: 56243

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDDNLKAQLRAYLENIRQPVELVASLDDGAKSKELLELLEEIAAASDKVRVVTGGDDAR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KPSFAIVRAGTDVSVRFAGLPLGHEFTSLVLTLLWVGGHPPKVEPEVIEQVKALDGDFSF
CCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCE
ETYFSLSCQNCPDVVQALNLMSVLNPKIRHVSIDGALFQDEVEKRQVMAVPTVLLNGEPF
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEECEEEECCCCC
AQGRMELGQILAKIDTGAQARAAEKIKGKDAFDVLVVGGGPAGAAAAIYAARKGIRTGLA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCH
AERFGGQVLDTLAIENFISVPHTEGPKLAAGLEQHVKDYDVDVMNLQKATKLVPAREPGG
HHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
LITVELENGASLKSRTVILATGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEEECCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLASLPNVRIVTSALTTEVKGDG
ECCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCC
QKVTGLVYKDRNSDEVHNVDLEGIFVQIGLVPNTEWLKDSVALSPRGEIETDYRGQTSIP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
GVFAAGDATTTPYKQIVIAMGEGSKAALSAFDYLIRQPVPAQSQAAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MLDDNLKAQLRAYLENIRQPVELVASLDDGAKSKELLELLEEIAAASDKVRVVTGGDDAR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCC
KPSFAIVRAGTDVSVRFAGLPLGHEFTSLVLTLLWVGGHPPKVEPEVIEQVKALDGDFSF
CCCEEEEEECCCEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCE
ETYFSLSCQNCPDVVQALNLMSVLNPKIRHVSIDGALFQDEVEKRQVMAVPTVLLNGEPF
EEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHEEEECEEEECCCCC
AQGRMELGQILAKIDTGAQARAAEKIKGKDAFDVLVVGGGPAGAAAAIYAARKGIRTGLA
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCH
AERFGGQVLDTLAIENFISVPHTEGPKLAAGLEQHVKDYDVDVMNLQKATKLVPAREPGG
HHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCC
LITVELENGASLKSRTVILATGARWRQMNVPGEDAYRNKGVAYCPHCDGPLFKGKRVAVI
EEEEEECCCCCCCCCEEEEEECCCEEECCCCCCHHHHCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLASLPNVRIVTSALTTEVKGDG
ECCCCCHHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCC
QKVTGLVYKDRNSDEVHNVDLEGIFVQIGLVPNTEWLKDSVALSPRGEIETDYRGQTSIP
CEEEEEEEECCCCCCEEEEEEEEEEEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCC
GVFAAGDATTTPYKQIVIAMGEGSKAALSAFDYLIRQPVPAQSQAAA
CEEECCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]