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The map label for this gene is menB [C]

Identifier: 197106025

GI number: 197106025

Start: 2945588

End: 2946367

Strand: Reverse

Name: menB [C]

Synonym: PHZ_c2564

Alternate gene names: 197106025

Gene position: 2946367-2945588 (Counterclockwise)

Preceding gene: 197106027

Following gene: 197106024

Centisome position: 73.73

GC content: 70.26

Gene sequence:

>780_bases
ATGCCGGAGTTGACCCAAGTCAGACTTCAGCGAGACGGCGCCCGTGCGACCCTGACCTTCCTCCGGCCGGCCGAGCTCAA
TGCGCTCTCGCCGACGTTGATCGCGGAGGCGCAGCGCTGCGTCAGCTCCGTCGCCCAGTCGGACGCCGGCGTGCTCGTCC
TGGCGGCCGAGGGGCGATCGTTCTCGGCCGGTGTGGACCTCAAGGCCGTCTCGTCGCCGGATTACACGCCGGAGGTCGCC
CGCCTCTTCTCGGAACAGGCGCGGGACCTGATCCGGCAATTGGAGACCATGCCGCAGGTGACGATCGCGCGGGTGACGGG
ACACTGCTTCACCGGCGGGCTGGAAGTGGCGCTGGGGTGCGATTTCATCGTCGCCGCGCAGGACGCGGTCTTCGCCGACA
CCCACGCGAAGCTGGGCTTCCGGGCCGGATGGGGCCTCAGCCAGCGCCTTCCGCGCCGCATCGGCCTGATGCGCGCGAAG
GAGATGTCCTTCACCGCCCGGCGCGTGTCTGCGGCCCAGGCGGTGGAACTGGGGCTCATCCTGGACGCCGTGCCGGCCGA
GGCGCTCGATCGCCGGATCGACGACCTGGTGAGCGCCGTGACGGAGAACAGCGCCGGCTCCATCGCCGCCTACAAGGTGC
TCTACGACAAGGCCCAGAACGAGGGGCTGGAGGCCGGGCTGACCTTCGAGGCTACGGTCAAGCTGCCGATCGCCGATCGG
GAGGCGAGGCTTGGCGCCACGACTCGCAGGCTGGGCTCGGGCTCCGGCCCGGCAAACTGA

Upstream 100 bases:

>100_bases
TGCGGTTCACAGTCAGGCTGCGATCATGCCTTCCATTATCTAACTGATCGAACTATTTCTTGGTGACGGCGCCATTGGCC
GTCGACTGGAGGAGCGACCC

Downstream 100 bases:

>100_bases
ATTCAGAGCTCAAGAACATAAGCGCTCGCATCGCAGCGAGCCGGTGAGGAAACGGCAGATGACGGCAGGTACGGCGGCGC
AGGGGAAGAGCGGCGAGACA

Product: enoyl-CoA hydratase/carnithine racemase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MPELTQVRLQRDGARATLTFLRPAELNALSPTLIAEAQRCVSSVAQSDAGVLVLAAEGRSFSAGVDLKAVSSPDYTPEVA
RLFSEQARDLIRQLETMPQVTIARVTGHCFTGGLEVALGCDFIVAAQDAVFADTHAKLGFRAGWGLSQRLPRRIGLMRAK
EMSFTARRVSAAQAVELGLILDAVPAEALDRRIDDLVSAVTENSAGSIAAYKVLYDKAQNEGLEAGLTFEATVKLPIADR
EARLGATTRRLGSGSGPAN

Sequences:

>Translated_259_residues
MPELTQVRLQRDGARATLTFLRPAELNALSPTLIAEAQRCVSSVAQSDAGVLVLAAEGRSFSAGVDLKAVSSPDYTPEVA
RLFSEQARDLIRQLETMPQVTIARVTGHCFTGGLEVALGCDFIVAAQDAVFADTHAKLGFRAGWGLSQRLPRRIGLMRAK
EMSFTARRVSAAQAVELGLILDAVPAEALDRRIDDLVSAVTENSAGSIAAYKVLYDKAQNEGLEAGLTFEATVKLPIADR
EARLGATTRRLGSGSGPAN
>Mature_258_residues
PELTQVRLQRDGARATLTFLRPAELNALSPTLIAEAQRCVSSVAQSDAGVLVLAAEGRSFSAGVDLKAVSSPDYTPEVAR
LFSEQARDLIRQLETMPQVTIARVTGHCFTGGLEVALGCDFIVAAQDAVFADTHAKLGFRAGWGLSQRLPRRIGLMRAKE
MSFTARRVSAAQAVELGLILDAVPAEALDRRIDDLVSAVTENSAGSIAAYKVLYDKAQNEGLEAGLTFEATVKLPIADRE
ARLGATTRRLGSGSGPAN

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=176, Percent_Identity=32.9545454545455, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194097323, Length=208, Percent_Identity=31.25, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI20127408, Length=162, Percent_Identity=31.4814814814815, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI68989263, Length=178, Percent_Identity=28.0898876404494, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI31542718, Length=246, Percent_Identity=30.4878048780488, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI116235487, Length=157, Percent_Identity=26.7515923566879, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI1788597, Length=192, Percent_Identity=29.6875, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1787659, Length=244, Percent_Identity=30.327868852459, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788682, Length=179, Percent_Identity=31.8435754189944, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790281, Length=176, Percent_Identity=31.8181818181818, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI221142681, Length=214, Percent_Identity=29.9065420560748, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1787660, Length=221, Percent_Identity=29.8642533936652, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI87082183, Length=199, Percent_Identity=26.6331658291457, Blast_Score=72, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=231, Percent_Identity=29.4372294372294, Blast_Score=87, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=186, Percent_Identity=30.1075268817204, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=229, Percent_Identity=26.6375545851528, Blast_Score=80, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=27.0386266094421, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17534483, Length=171, Percent_Identity=29.8245614035088, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17558304, Length=178, Percent_Identity=26.4044943820225, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=150, Percent_Identity=34.6666666666667, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24653139, Length=250, Percent_Identity=31.6, Blast_Score=91, Evalue=6e-19,
Organism=Drosophila melanogaster, GI20129971, Length=214, Percent_Identity=29.9065420560748, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24653477, Length=214, Percent_Identity=29.9065420560748, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922422, Length=210, Percent_Identity=25.7142857142857, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19920382, Length=172, Percent_Identity=30.2325581395349, Blast_Score=77, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 27552; Mature: 27421

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELTQVRLQRDGARATLTFLRPAELNALSPTLIAEAQRCVSSVAQSDAGVLVLAAEGRS
CCCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
FSAGVDLKAVSSPDYTPEVARLFSEQARDLIRQLETMPQVTIARVTGHCFTGGLEVALGC
EECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCEEEEECC
DFIVAAQDAVFADTHAKLGFRAGWGLSQRLPRRIGLMRAKEMSFTARRVSAAQAVELGLI
CEEEECCCHHEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAVPAEALDRRIDDLVSAVTENSAGSIAAYKVLYDKAQNEGLEAGLTFEATVKLPIADR
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
EARLGATTRRLGSGSGPAN
HHHCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
PELTQVRLQRDGARATLTFLRPAELNALSPTLIAEAQRCVSSVAQSDAGVLVLAAEGRS
CCHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
FSAGVDLKAVSSPDYTPEVARLFSEQARDLIRQLETMPQVTIARVTGHCFTGGLEVALGC
EECCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCEEEEECC
DFIVAAQDAVFADTHAKLGFRAGWGLSQRLPRRIGLMRAKEMSFTARRVSAAQAVELGLI
CEEEECCCHHEECCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDAVPAEALDRRIDDLVSAVTENSAGSIAAYKVLYDKAQNEGLEAGLTFEATVKLPIADR
HHHCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCC
EARLGATTRRLGSGSGPAN
HHHCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA