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The map label for this gene is rspA [H]

Identifier: 197105937

GI number: 197105937

Start: 2837115

End: 2838326

Strand: Direct

Name: rspA [H]

Synonym: PHZ_c2476

Alternate gene names: 197105937

Gene position: 2837115-2838326 (Clockwise)

Preceding gene: 197105935

Following gene: 197105938

Centisome position: 70.99

GC content: 70.21

Gene sequence:

>1212_bases
ATGCCCAAGATCACCTCAGCCAAGACGATCGTGACCTGCCCCGGGCGCAACTTCGTCACGCTGAAGATCGAGACCTCCGA
CGGGGTCACGGGCGTGGGCGACGCCACCCTGAACGGCCGCGAGCTGGCGGTCGCCAGCTATCTCGACGACCACGTCATCC
CCTGCCTGATCGGCCGCGACGCGCACCAGATCGAGGACATCTGGCAGTACCTGCATCGGGGCGCCTACTGGCGCCGGGGT
CCGGTGACCATGAGCGCCATCGCCGCCGTCGACATGGCGCTGTGGGACATCAAGGGGAAGATCGCCGGCCTGCCGGTCTA
CCAACTCCTCGGCGGCGCCTGCCGCACCGGCGTCATGGTCTACGGCCACGCCAACGGCCAGACCATCGAGGAGACCGTCG
AGCGCGCCAGGGAGTACGTCCAGGCCGGCTACAGGGCCGTGCGGCTGCAGTCTGGCGTGCCCGGCCTGAAGGCCGCCTAC
GGCGTCTCCAAGGACAAGTTCTTCTACGAGCCCGCCGACGCCGACCTGCCCACCGAGACCGTGTGGTCCAGCGAGGCGTA
CATGCGCGTGGCGCCCGAGCTGTTCGCCGCGGCCCGCCAGGCCCTGGGCTGGGACGTCCACCTGCTGCACGACGTCCACC
ACCGGCTGACGCCGATCGAGGCCGGCCGCCTGGGCCGCGACCTGGAGCCCTACCGGCCCTTCTGGATGGAGGATCCGACG
CCGGCCGAGAACCAGGCCCGCTTCCGCCTGATCCGCCAGCACACCACAACCCCGATCGCGGTCGGCGAGGTGTTCAACTC
GATCTGGGACTGCAAGCAGCTGATCGAGGAGCAGCTGATCGACTACATCCGCGCCACGGTCGTCCACGCCGGCGGCCTCA
CCCACCTGCGCCGGATCGCCAGCTTCGCCGACCTCTACGGCGTGCGCACCGGCTGCCACGGGGCCACGGACCTGTCGCCG
GTGACCATGGCCGCGGCCCTGCACTTCGACATCTCGGTCCCGAACTTCGGGGTGCAGGAATACATGCGCCACACGCCCGA
GACCGACGCCGTCTTCCCCCACGCCTACCGCTTCCAGGACGGCATGCTCCACCCGGGCGAAGCGCCCGGCCTGGGCGTGG
ACATCGACGAGGCCGAAGCCGCCCGCCACCCCTACCGGCGCGCCTATTTGCCGGTGGCGCGGCTGGCGGACGGCACGATG
CACGACTGGTGA

Upstream 100 bases:

>100_bases
ACACCACCCTTCTAGTGGTCTGACCATGTGATTGACAACACCGGGCCCGAGACTAAGCTTGGACGACCTCGCGGCTCCCC
CCGCCCTTCCCCGAAAGCCG

Downstream 100 bases:

>100_bases
GGGCGCGCCCCTTCTGTCCCGCGGGAATGGTGGAGTATGGTTGCGCGTAGAATCCCCCCGTCGGGCGGCAAGGCGAAAAG
CCAGGAGACGATGGCCAGCG

Product: mandelate racemase/muconate lactonizing enzyme family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MPKITSAKTIVTCPGRNFVTLKIETSDGVTGVGDATLNGRELAVASYLDDHVIPCLIGRDAHQIEDIWQYLHRGAYWRRG
PVTMSAIAAVDMALWDIKGKIAGLPVYQLLGGACRTGVMVYGHANGQTIEETVERAREYVQAGYRAVRLQSGVPGLKAAY
GVSKDKFFYEPADADLPTETVWSSEAYMRVAPELFAAARQALGWDVHLLHDVHHRLTPIEAGRLGRDLEPYRPFWMEDPT
PAENQARFRLIRQHTTTPIAVGEVFNSIWDCKQLIEEQLIDYIRATVVHAGGLTHLRRIASFADLYGVRTGCHGATDLSP
VTMAAALHFDISVPNFGVQEYMRHTPETDAVFPHAYRFQDGMLHPGEAPGLGVDIDEAEAARHPYRRAYLPVARLADGTM
HDW

Sequences:

>Translated_403_residues
MPKITSAKTIVTCPGRNFVTLKIETSDGVTGVGDATLNGRELAVASYLDDHVIPCLIGRDAHQIEDIWQYLHRGAYWRRG
PVTMSAIAAVDMALWDIKGKIAGLPVYQLLGGACRTGVMVYGHANGQTIEETVERAREYVQAGYRAVRLQSGVPGLKAAY
GVSKDKFFYEPADADLPTETVWSSEAYMRVAPELFAAARQALGWDVHLLHDVHHRLTPIEAGRLGRDLEPYRPFWMEDPT
PAENQARFRLIRQHTTTPIAVGEVFNSIWDCKQLIEEQLIDYIRATVVHAGGLTHLRRIASFADLYGVRTGCHGATDLSP
VTMAAALHFDISVPNFGVQEYMRHTPETDAVFPHAYRFQDGMLHPGEAPGLGVDIDEAEAARHPYRRAYLPVARLADGTM
HDW
>Mature_402_residues
PKITSAKTIVTCPGRNFVTLKIETSDGVTGVGDATLNGRELAVASYLDDHVIPCLIGRDAHQIEDIWQYLHRGAYWRRGP
VTMSAIAAVDMALWDIKGKIAGLPVYQLLGGACRTGVMVYGHANGQTIEETVERAREYVQAGYRAVRLQSGVPGLKAAYG
VSKDKFFYEPADADLPTETVWSSEAYMRVAPELFAAARQALGWDVHLLHDVHHRLTPIEAGRLGRDLEPYRPFWMEDPTP
AENQARFRLIRQHTTTPIAVGEVFNSIWDCKQLIEEQLIDYIRATVVHAGGLTHLRRIASFADLYGVRTGCHGATDLSPV
TMAAALHFDISVPNFGVQEYMRHTPETDAVFPHAYRFQDGMLHPGEAPGLGVDIDEAEAARHPYRRAYLPVARLADGTMH
DW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=403, Percent_Identity=66.0049627791563, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI48994953, Length=412, Percent_Identity=31.0679611650485, Blast_Score=180, Evalue=2e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 44754; Mature: 44623

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00908 MR_MLE_1 ; PS00909 MR_MLE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKITSAKTIVTCPGRNFVTLKIETSDGVTGVGDATLNGRELAVASYLDDHVIPCLIGRD
CCCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCC
AHQIEDIWQYLHRGAYWRRGPVTMSAIAAVDMALWDIKGKIAGLPVYQLLGGACRTGVMV
HHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCEEE
YGHANGQTIEETVERAREYVQAGYRAVRLQSGVPGLKAAYGVSKDKFFYEPADADLPTET
EECCCCCHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHCCCCCCEEECCCCCCCCCHH
VWSSEAYMRVAPELFAAARQALGWDVHLLHDVHHRLTPIEAGRLGRDLEPYRPFWMEDPT
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCC
PAENQARFRLIRQHTTTPIAVGEVFNSIWDCKQLIEEQLIDYIRATVVHAGGLTHLRRIA
CCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFADLYGVRTGCHGATDLSPVTMAAALHFDISVPNFGVQEYMRHTPETDAVFPHAYRFQD
HHHHHHHHHCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHCCCC
GMLHPGEAPGLGVDIDEAEAARHPYRRAYLPVARLADGTMHDW
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHCHHHHHCCCCCCCC
>Mature Secondary Structure 
PKITSAKTIVTCPGRNFVTLKIETSDGVTGVGDATLNGRELAVASYLDDHVIPCLIGRD
CCCCCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEECCC
AHQIEDIWQYLHRGAYWRRGPVTMSAIAAVDMALWDIKGKIAGLPVYQLLGGACRTGVMV
HHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHCHHHHCCEEE
YGHANGQTIEETVERAREYVQAGYRAVRLQSGVPGLKAAYGVSKDKFFYEPADADLPTET
EECCCCCHHHHHHHHHHHHHHHCHHEEEECCCCCCHHHHHCCCCCCEEECCCCCCCCCHH
VWSSEAYMRVAPELFAAARQALGWDVHLLHDVHHRLTPIEAGRLGRDLEPYRPFWMEDPT
HCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCC
PAENQARFRLIRQHTTTPIAVGEVFNSIWDCKQLIEEQLIDYIRATVVHAGGLTHLRRIA
CCCCHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
SFADLYGVRTGCHGATDLSPVTMAAALHFDISVPNFGVQEYMRHTPETDAVFPHAYRFQD
HHHHHHHHHCCCCCCCCCCHHHHHEEEEEEECCCCCCHHHHHHCCCCCCCCCCCHHCCCC
GMLHPGEAPGLGVDIDEAEAARHPYRRAYLPVARLADGTMHDW
CCCCCCCCCCCCCCCCHHHHHHCCHHHHHCHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]