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The map label for this gene is hyuA [H]

Identifier: 197105832

GI number: 197105832

Start: 2686203

End: 2688254

Strand: Reverse

Name: hyuA [H]

Synonym: PHZ_c2371

Alternate gene names: 197105832

Gene position: 2688254-2686203 (Counterclockwise)

Preceding gene: 197105833

Following gene: 197105831

Centisome position: 67.27

GC content: 66.91

Gene sequence:

>2052_bases
ATGGCTAAGGGTATGTATCGTATTGGCGTCGATGTAGGTGGAACGTTCACCGACTTCACGATCGAGAACGTGAGCAGCGG
CGACTTTCTCTACTTCAAGCTTTCGTCCACGCCGGACGACCCGTCCGACGCCATCGTCAACGGCATCTCCCAGATCCTGG
AGCTGTTCCAGATCGGGGCGGCCGAGATTTCCTACATCGGCCACGGCACGACCGTCGCGACCAACATGATCATCGAGCGG
CGTGGGGTGCCGACCGGCCTCATCACCACCCAGGGCTTCCGGGATGTCCTGGCGATCGGGCGCCAGACGCGACCCGCCCT
CTACGACGTCACCGTTAAACGCCCGCCGCCTCTGGTGGAGCGCTACCGCCGGCTGGAAGTCGCTGAGCGACTGAATGCAA
AGGGCGAGGAGCTCTCCCCACTCGACGAGGCGGAACTCGAGCGGGTGCTCGACCAGCTTGCGGCCACGGGTGTCGAGGCG
ATCGCGATATCGTTCCTCCACGCGTACAAGAACGCCGAACATGAGGTGGCCGCGGCCCGGATCGCCGCCCGCAAGCTTCC
AGGCGTGTATGTAAGCACCTCGTCGAGCGTGCTCCCTGAATTCCGCGAGTTCGAGCGGACGTCGACGACCGTCCTCAACG
CCTATGTCGGCCCGAGGATGGCGGCCTATTTCGACAGGCTGAAGGCCAAGATCGCCGAGCGGGGCATCTCCGTGGCTCCG
ATGATCATTCACTCGAACGGCGGGCTGCTCTCCATCGACAGCGCAAAGCGCCTGCCGGTCACGACCTGCCTCTCCGGCCC
GGCAGCCGGCGTGATCGGCGCTGCCGTCGTGGGGGCTGCCGCTGGAGTGCGGGATCTGGTCACGTTCGACGTGGGAGGCA
CGAGCACCGATGTCTCGCTGGTCGTCGACGGCAAGCCCAAACTCGCCTCCAGTCGCGAGGTCGCCGGCTACCCCGTCCGT
CTGCCGATGATCGACATTCATGTGATCGGGGCCGGGGGCGGAAGCATCGCGCAGGTCGACGACGCCCAGGCTCTCAAGGT
TGGTCCGCGCAGCGCCGGCGCGCATCCCGGTCCCGTGGCCTACGGGCGCGGCGGCGACCTGCCAACGCTGACTGACGCCA
ACATCTGCCTGAACAGGCTCAACCCCGTCGCGCTTCTTGACGGCCGCATGCCGGTGGATCGAGAGGCCGCCTGGACGAGC
ATCGAGGAGAAGATCGCGCGGCCGCTCGGCATCTCGGTTGAGAAGGCCGCCTACGGCGTCATCCAGGTCGCCGCCGCCAA
CATGAGCCGCGCCATTCGAGCGGTCACGACGGAGCAGGGACACGATGTCGGGGACATCGCTCTCTTCGCGTTCGGCGGCG
CCGGTCCTCTGCATGCCTCCGAGGTGGCTCGCGAGTCGGGGATCCGCCGCATTCTCGTGCCGCAGGAACCCGGTACGATG
TGCGCTCGGGGCGTGTTGTTGTCGGACGTGAGCCTCGACTTTGTGCGGATGCAGCTGACGGCCGCGACGCAGTCCTCCTG
GGAGCGCGCCTGCGCGGCCTTGCGCGAGATGGCGGATGAGGGCGACCGGTGGCTGGCCGGCGAGAATGTCGAGCCCGAGC
TGCGCCGCGCGGAGCTCGCGATCGACGCTCACTACGCTGGCCAGAGCCACGAACTGCGCGTGCCGCTTGAGGCGGCAGAC
CCGTCGGGCCTCGACGATTTCCTCGCCCGGTTCGGCAACGCGCACGAGGCCGCCTTCGGATACCAGAACGCCGGCCAGCC
GGTGTTTCTGGTGAGCTGCCGTCTGCGCGCGGTCGGCGTTGTTCCCAAGCAGCTTGAGCCCAGTCACAAGGGCGGCTCGA
GCCTCGCGGCGGCAGAGACGGACGTGCGTTCCGTCTACTACGGGCCCGAGGAAGGCTGGCTTCAGACGCCGATTTTCCGT
CGGTCCGCCCTGCCGGTCGGTACGAACGTCGTGGGACCCTGCGTCATCGAGGAAATGAGCTCCACGACCGTGGTGCTCCG
CGGTCAGACCGCCACGATCGACGCCTACGGCAACATCATCATCACGAGCTGA

Upstream 100 bases:

>100_bases
TGGACCTCAATGTCGCGCGCGTTACGGCGCGCGTAGCTCCCAGGCGGCAGGTGTTTCTGAGCTGAGCCGATGAAGGCTGG
CGACGTCACCGGGAAGAAAG

Downstream 100 bases:

>100_bases
GCATCAGGCTATGGCAGAGAGAACGCAAACCCAGGACGCCGCGCCGCAACGCCAGTTCGATGCGATCGCGATGGAAGTCT
TCAGCAATCGCATGCTCTCG

Product: N-methylhydantoinase A/acetone carboxylase, beta subunit

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 683; Mature: 682

Protein sequence:

>683_residues
MAKGMYRIGVDVGGTFTDFTIENVSSGDFLYFKLSSTPDDPSDAIVNGISQILELFQIGAAEISYIGHGTTVATNMIIER
RGVPTGLITTQGFRDVLAIGRQTRPALYDVTVKRPPPLVERYRRLEVAERLNAKGEELSPLDEAELERVLDQLAATGVEA
IAISFLHAYKNAEHEVAAARIAARKLPGVYVSTSSSVLPEFREFERTSTTVLNAYVGPRMAAYFDRLKAKIAERGISVAP
MIIHSNGGLLSIDSAKRLPVTTCLSGPAAGVIGAAVVGAAAGVRDLVTFDVGGTSTDVSLVVDGKPKLASSREVAGYPVR
LPMIDIHVIGAGGGSIAQVDDAQALKVGPRSAGAHPGPVAYGRGGDLPTLTDANICLNRLNPVALLDGRMPVDREAAWTS
IEEKIARPLGISVEKAAYGVIQVAAANMSRAIRAVTTEQGHDVGDIALFAFGGAGPLHASEVARESGIRRILVPQEPGTM
CARGVLLSDVSLDFVRMQLTAATQSSWERACAALREMADEGDRWLAGENVEPELRRAELAIDAHYAGQSHELRVPLEAAD
PSGLDDFLARFGNAHEAAFGYQNAGQPVFLVSCRLRAVGVVPKQLEPSHKGGSSLAAAETDVRSVYYGPEEGWLQTPIFR
RSALPVGTNVVGPCVIEEMSSTTVVLRGQTATIDAYGNIIITS

Sequences:

>Translated_683_residues
MAKGMYRIGVDVGGTFTDFTIENVSSGDFLYFKLSSTPDDPSDAIVNGISQILELFQIGAAEISYIGHGTTVATNMIIER
RGVPTGLITTQGFRDVLAIGRQTRPALYDVTVKRPPPLVERYRRLEVAERLNAKGEELSPLDEAELERVLDQLAATGVEA
IAISFLHAYKNAEHEVAAARIAARKLPGVYVSTSSSVLPEFREFERTSTTVLNAYVGPRMAAYFDRLKAKIAERGISVAP
MIIHSNGGLLSIDSAKRLPVTTCLSGPAAGVIGAAVVGAAAGVRDLVTFDVGGTSTDVSLVVDGKPKLASSREVAGYPVR
LPMIDIHVIGAGGGSIAQVDDAQALKVGPRSAGAHPGPVAYGRGGDLPTLTDANICLNRLNPVALLDGRMPVDREAAWTS
IEEKIARPLGISVEKAAYGVIQVAAANMSRAIRAVTTEQGHDVGDIALFAFGGAGPLHASEVARESGIRRILVPQEPGTM
CARGVLLSDVSLDFVRMQLTAATQSSWERACAALREMADEGDRWLAGENVEPELRRAELAIDAHYAGQSHELRVPLEAAD
PSGLDDFLARFGNAHEAAFGYQNAGQPVFLVSCRLRAVGVVPKQLEPSHKGGSSLAAAETDVRSVYYGPEEGWLQTPIFR
RSALPVGTNVVGPCVIEEMSSTTVVLRGQTATIDAYGNIIITS
>Mature_682_residues
AKGMYRIGVDVGGTFTDFTIENVSSGDFLYFKLSSTPDDPSDAIVNGISQILELFQIGAAEISYIGHGTTVATNMIIERR
GVPTGLITTQGFRDVLAIGRQTRPALYDVTVKRPPPLVERYRRLEVAERLNAKGEELSPLDEAELERVLDQLAATGVEAI
AISFLHAYKNAEHEVAAARIAARKLPGVYVSTSSSVLPEFREFERTSTTVLNAYVGPRMAAYFDRLKAKIAERGISVAPM
IIHSNGGLLSIDSAKRLPVTTCLSGPAAGVIGAAVVGAAAGVRDLVTFDVGGTSTDVSLVVDGKPKLASSREVAGYPVRL
PMIDIHVIGAGGGSIAQVDDAQALKVGPRSAGAHPGPVAYGRGGDLPTLTDANICLNRLNPVALLDGRMPVDREAAWTSI
EEKIARPLGISVEKAAYGVIQVAAANMSRAIRAVTTEQGHDVGDIALFAFGGAGPLHASEVARESGIRRILVPQEPGTMC
ARGVLLSDVSLDFVRMQLTAATQSSWERACAALREMADEGDRWLAGENVEPELRRAELAIDAHYAGQSHELRVPLEAADP
SGLDDFLARFGNAHEAAFGYQNAGQPVFLVSCRLRAVGVVPKQLEPSHKGGSSLAAAETDVRSVYYGPEEGWLQTPIFRR
SALPVGTNVVGPCVIEEMSSTTVVLRGQTATIDAYGNIIITS

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=731, Percent_Identity=30.9165526675787, Blast_Score=235, Evalue=9e-62,
Organism=Caenorhabditis elegans, GI133901900, Length=703, Percent_Identity=27.7382645803698, Blast_Score=216, Evalue=3e-56,
Organism=Saccharomyces cerevisiae, GI6322634, Length=734, Percent_Identity=30.1089918256131, Blast_Score=279, Evalue=8e-76,
Organism=Drosophila melanogaster, GI45550492, Length=735, Percent_Identity=30.4761904761905, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 72469; Mature: 72338

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKGMYRIGVDVGGTFTDFTIENVSSGDFLYFKLSSTPDDPSDAIVNGISQILELFQIGA
CCCCEEEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
AEISYIGHGTTVATNMIIERRGVPTGLITTQGFRDVLAIGRQTRPALYDVTVKRPPPLVE
HHEEEECCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEEECCCCHHHH
RYRRLEVAERLNAKGEELSPLDEAELERVLDQLAATGVEAIAISFLHAYKNAEHEVAAAR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
IAARKLPGVYVSTSSSVLPEFREFERTSTTVLNAYVGPRMAAYFDRLKAKIAERGISVAP
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHHHCCCCEEE
MIIHSNGGLLSIDSAKRLPVTTCLSGPAAGVIGAAVVGAAAGVRDLVTFDVGGTSTDVSL
EEEECCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEE
VVDGKPKLASSREVAGYPVRLPMIDIHVIGAGGGSIAQVDDAQALKVGPRSAGAHPGPVA
EECCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEECCCCCCCCCCCCC
YGRGGDLPTLTDANICLNRLNPVALLDGRMPVDREAAWTSIEEKIARPLGISVEKAAYGV
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
IQVAAANMSRAIRAVTTEQGHDVGDIALFAFGGAGPLHASEVARESGIRRILVPQEPGTM
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCHH
CARGVLLSDVSLDFVRMQLTAATQSSWERACAALREMADEGDRWLAGENVEPELRRAELA
HHCCHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHEEE
IDAHYAGQSHELRVPLEAADPSGLDDFLARFGNAHEAAFGYQNAGQPVFLVSCRLRAVGV
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEEHEECC
VPKQLEPSHKGGSSLAAAETDVRSVYYGPEEGWLQTPIFRRSALPVGTNVVGPCVIEEMS
CCHHCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
STTVVLRGQTATIDAYGNIIITS
CCEEEEECCEEEEEECCCEEEEC
>Mature Secondary Structure 
AKGMYRIGVDVGGTFTDFTIENVSSGDFLYFKLSSTPDDPSDAIVNGISQILELFQIGA
CCCEEEEEEECCCCEEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
AEISYIGHGTTVATNMIIERRGVPTGLITTQGFRDVLAIGRQTRPALYDVTVKRPPPLVE
HHEEEECCCHHHHHHHHHCCCCCCCCEEECCCHHHHHHHCCCCCCEEEEEEECCCCHHHH
RYRRLEVAERLNAKGEELSPLDEAELERVLDQLAATGVEAIAISFLHAYKNAEHEVAAAR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHH
IAARKLPGVYVSTSSSVLPEFREFERTSTTVLNAYVGPRMAAYFDRLKAKIAERGISVAP
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHEEEHHHCHHHHHHHHHHHHHHHHCCCCEEE
MIIHSNGGLLSIDSAKRLPVTTCLSGPAAGVIGAAVVGAAAGVRDLVTFDVGGTSTDVSL
EEEECCCCEEEECCCCCCCEEEECCCCCHHHHHHHHHHHHCCHHEEEEEECCCCCCEEEE
VVDGKPKLASSREVAGYPVRLPMIDIHVIGAGGGSIAQVDDAQALKVGPRSAGAHPGPVA
EECCCCCCCCCCCCCCCEEEEEEEEEEEEECCCCCEEECCCCCCEEECCCCCCCCCCCCC
YGRGGDLPTLTDANICLNRLNPVALLDGRMPVDREAAWTSIEEKIARPLGISVEKAAYGV
CCCCCCCCCCCCHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
IQVAAANMSRAIRAVTTEQGHDVGDIALFAFGGAGPLHASEVARESGIRRILVPQEPGTM
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCHHHHHHHCCCEEEEECCCCCHH
CARGVLLSDVSLDFVRMQLTAATQSSWERACAALREMADEGDRWLAGENVEPELRRAELA
HHCCHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCHHHHHHEEE
IDAHYAGQSHELRVPLEAADPSGLDDFLARFGNAHEAAFGYQNAGQPVFLVSCRLRAVGV
EEEEECCCCCEEEEEECCCCCCCHHHHHHHHCCCHHHHCCCCCCCCEEEEEEEEEHEECC
VPKQLEPSHKGGSSLAAAETDVRSVYYGPEEGWLQTPIFRRSALPVGTNVVGPCVIEEMS
CCHHCCCCCCCCCCCCHHHCCCHHEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHCCC
STTVVLRGQTATIDAYGNIIITS
CCEEEEECCEEEEEECCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]