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The map label for this gene is mrdA [H]

Identifier: 197105743

GI number: 197105743

Start: 2584641

End: 2586647

Strand: Reverse

Name: mrdA [H]

Synonym: PHZ_c2281

Alternate gene names: 197105743

Gene position: 2586647-2584641 (Counterclockwise)

Preceding gene: 197105744

Following gene: 197105742

Centisome position: 64.73

GC content: 70.85

Gene sequence:

>2007_bases
ATGGCCGAACCGGCAATCTTCTTCGAGGAGGTCAACGAGCGGCAGGGCCTGTTCCACCGCCGGGCCGTCCTGCTGGGCGG
CTTCGCGGGCCTGGGCCTCACGGCCCTGGGCGGGCGCCTGGCCTATCTCCAGCTGCTGGAGACCCAGCGCTACGAGAAGC
TGGCCACCTCGAACCAGTTCAACTTCAAGCTGGTCCCGGCTCCCCGCGGCCTGATCGTCGACCGCAACGGCGCGGTGCTG
GCGGCGAACCGCCCGAACTTCCGCCTGCTGGTGTCGAAGGACAAGGGGACCGACCCCGAGGCGACGCTGAAGACCCTGGC
CAGCTTCGTGCCGATGGACGAGGCCCGCCAGCGCCGGCTGCTGCGCGAGATCAGCAACGCGCCCCGCCGTGCGCCGGTCT
CGGTCATGGAGGACATGACCTGGGAGCAGTTCTCCGCGATCAACGTCCGCGCGCCCGAGCTGCCGAACGTCACCGCCGAC
ATGGGCGAGGTGCGGGTCTATCCGCACGGCGGGGCCTTCGCGCACGTGATCGGCTACGTGGCCAAGGTGAACCGCGAGGA
CCTGACGCCCACCGGCCCGAACTCGGACCCCATCCTGCTGCACCCGGGCTTCCGGATCGGCAAGCAGGGCATCGAGAAGG
CGCTCGACCTCGAGCTGCGGGGCCGGGCCGGCGCCAAGAAGGTCGAGGTGGACGCCAACGGCCGCGAGGTGCGCCACGAC
GAGGACGGCGACATCGCCGCGGTCCCCGGCAAGGAGGTCCAGCTCACCCTCGACGTCGATATCCAGAACCGCGCCCTCGA
GGTGTTCGGCGGCGAGAGCGGAGGCGCGGTCCTGATGGACTGCCGCACGGGCGACATCCTGTGCATGATGTCGGCGCCGA
GCTTCGATGCGAACCGGTTCGTGCGGGGGCTGACGGGCGCCGAGTACCGTGCGCTGGCCAACTACGAACGCCGGCCGCTG
CTCGACAAGTGCCTGTCGGGCACCTACCCGCCGGGCTCGACCTTCAAGACCATGACGGCGCTCGCGGCGCTGGAGGCCGG
GATCGACCCCGACCAGACCGTCAGCTGCGGCGGCGGAATGTACTTCGGCGGCCGCTACTTCCGCTGCCACAAGAAGGGCG
GCCACGGCGCCCAGAACATGCGGGACGCCATCAAGAACTCCTGCGACGTCTATTTCTATTCGGTCGCCTCGCGCATCGGG
CCGGACCGGATCGCCGCCGTGGCGCGCGAATTCGGCTTCGGCCAGACCTTCGACATTGGCATTCCGGGCCAGAAGAAGGG
CATCGTGCCCTCGACCCAGTGGGTCCGGGAGAACCGCCCGAAGGACCCCAAGTGGCACCCCGGTGAGTCGCTGAGCTACG
GCATCGGCCAGGGCATGCTGACGGCCAATTGCCTGCAACTGGCGGTGATGACCGCGCGGCTGGCCAACGGCCGCAAGGCG
CTCAACCCGCGCCTCATCAAGTCGATCGGCGGCAAGGAGCTGCCGCGCGGCTCGGACGTGCCGGACCTGCCGTTCAGCCA
GGAGCACCTGAACTACGTCCGCGACGGCATGGCCGCGGTGACCGAGGCGGGCGGGACGGCCTTCCGAGCCAGCCAGCTCG
GCCTGGGCGACATCCTGATGGCCGGCAAGACCGGCACGGCGCAGGTCTACAGCTACAAGGCCGGCGGGCACCGGGGGAAG
GGCGGCCCCTGGCGGCTGCGCGACCACGGCCTGTTCGTCGCCTTCGCGCCGCTGGATGAGCCGCGCTACGCCCTGGCGGT
GATCGTCCAGCACGGCATGGGCGGCTCGACGGCCGCCGCGCCCCGGGCGCGCGAGATCATGCGGGTGGCCCTGCTGAAGG
ATCCCGAGCTGCGCGCGCGCATCGAGACGCCGCTGCCGATGCCCGAGATCCAGCCGGACCTGGAGCTCGACACCGTGCCC
GACAGCCCGGTCTTCGCCGAGCCGCCGAACACGCCGACGCCCGCCGTCGGCGATCCGCTCGTCCCGCCCTCGGAAGGCCC
CGCATGA

Upstream 100 bases:

>100_bases
TCGGTGTTCTGGCAATTCCTCGCCACAGTCATCCTCTATCCCTTCGCCGACCGCCTGATCGAGCGGTTCGAGGACGCCGA
CGTGAGGTTCCGCTAGCGCC

Downstream 100 bases:

>100_bases
CCGTCTCGGCCCTCACGCGTCCCGGCGAGCGGGACCGCCTGATCGTCAAGCTGTCCGAGGTGGACTGGCTGTTCGTCCTG
GTGCTCAGCCTGATCGCCGG

Product: penicillin-binding protein 2

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MAEPAIFFEEVNERQGLFHRRAVLLGGFAGLGLTALGGRLAYLQLLETQRYEKLATSNQFNFKLVPAPRGLIVDRNGAVL
AANRPNFRLLVSKDKGTDPEATLKTLASFVPMDEARQRRLLREISNAPRRAPVSVMEDMTWEQFSAINVRAPELPNVTAD
MGEVRVYPHGGAFAHVIGYVAKVNREDLTPTGPNSDPILLHPGFRIGKQGIEKALDLELRGRAGAKKVEVDANGREVRHD
EDGDIAAVPGKEVQLTLDVDIQNRALEVFGGESGGAVLMDCRTGDILCMMSAPSFDANRFVRGLTGAEYRALANYERRPL
LDKCLSGTYPPGSTFKTMTALAALEAGIDPDQTVSCGGGMYFGGRYFRCHKKGGHGAQNMRDAIKNSCDVYFYSVASRIG
PDRIAAVAREFGFGQTFDIGIPGQKKGIVPSTQWVRENRPKDPKWHPGESLSYGIGQGMLTANCLQLAVMTARLANGRKA
LNPRLIKSIGGKELPRGSDVPDLPFSQEHLNYVRDGMAAVTEAGGTAFRASQLGLGDILMAGKTGTAQVYSYKAGGHRGK
GGPWRLRDHGLFVAFAPLDEPRYALAVIVQHGMGGSTAAAPRAREIMRVALLKDPELRARIETPLPMPEIQPDLELDTVP
DSPVFAEPPNTPTPAVGDPLVPPSEGPA

Sequences:

>Translated_668_residues
MAEPAIFFEEVNERQGLFHRRAVLLGGFAGLGLTALGGRLAYLQLLETQRYEKLATSNQFNFKLVPAPRGLIVDRNGAVL
AANRPNFRLLVSKDKGTDPEATLKTLASFVPMDEARQRRLLREISNAPRRAPVSVMEDMTWEQFSAINVRAPELPNVTAD
MGEVRVYPHGGAFAHVIGYVAKVNREDLTPTGPNSDPILLHPGFRIGKQGIEKALDLELRGRAGAKKVEVDANGREVRHD
EDGDIAAVPGKEVQLTLDVDIQNRALEVFGGESGGAVLMDCRTGDILCMMSAPSFDANRFVRGLTGAEYRALANYERRPL
LDKCLSGTYPPGSTFKTMTALAALEAGIDPDQTVSCGGGMYFGGRYFRCHKKGGHGAQNMRDAIKNSCDVYFYSVASRIG
PDRIAAVAREFGFGQTFDIGIPGQKKGIVPSTQWVRENRPKDPKWHPGESLSYGIGQGMLTANCLQLAVMTARLANGRKA
LNPRLIKSIGGKELPRGSDVPDLPFSQEHLNYVRDGMAAVTEAGGTAFRASQLGLGDILMAGKTGTAQVYSYKAGGHRGK
GGPWRLRDHGLFVAFAPLDEPRYALAVIVQHGMGGSTAAAPRAREIMRVALLKDPELRARIETPLPMPEIQPDLELDTVP
DSPVFAEPPNTPTPAVGDPLVPPSEGPA
>Mature_667_residues
AEPAIFFEEVNERQGLFHRRAVLLGGFAGLGLTALGGRLAYLQLLETQRYEKLATSNQFNFKLVPAPRGLIVDRNGAVLA
ANRPNFRLLVSKDKGTDPEATLKTLASFVPMDEARQRRLLREISNAPRRAPVSVMEDMTWEQFSAINVRAPELPNVTADM
GEVRVYPHGGAFAHVIGYVAKVNREDLTPTGPNSDPILLHPGFRIGKQGIEKALDLELRGRAGAKKVEVDANGREVRHDE
DGDIAAVPGKEVQLTLDVDIQNRALEVFGGESGGAVLMDCRTGDILCMMSAPSFDANRFVRGLTGAEYRALANYERRPLL
DKCLSGTYPPGSTFKTMTALAALEAGIDPDQTVSCGGGMYFGGRYFRCHKKGGHGAQNMRDAIKNSCDVYFYSVASRIGP
DRIAAVAREFGFGQTFDIGIPGQKKGIVPSTQWVRENRPKDPKWHPGESLSYGIGQGMLTANCLQLAVMTARLANGRKAL
NPRLIKSIGGKELPRGSDVPDLPFSQEHLNYVRDGMAAVTEAGGTAFRASQLGLGDILMAGKTGTAQVYSYKAGGHRGKG
GPWRLRDHGLFVAFAPLDEPRYALAVIVQHGMGGSTAAAPRAREIMRVALLKDPELRARIETPLPMPEIQPDLELDTVPD
SPVFAEPPNTPTPAVGDPLVPPSEGPA

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=630, Percent_Identity=32.3809523809524, Blast_Score=283, Evalue=4e-77,
Organism=Escherichia coli, GI1786272, Length=605, Percent_Identity=23.801652892562, Blast_Score=114, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 72263; Mature: 72132

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEPAIFFEEVNERQGLFHRRAVLLGGFAGLGLTALGGRLAYLQLLETQRYEKLATSNQF
CCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
NFKLVPAPRGLIVDRNGAVLAANRPNFRLLVSKDKGTDPEATLKTLASFVPMDEARQRRL
EEEEECCCCCEEEECCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHH
LREISNAPRRAPVSVMEDMTWEQFSAINVRAPELPNVTADMGEVRVYPHGGAFAHVIGYV
HHHHHCCCCCCCHHHHHCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
AKVNREDLTPTGPNSDPILLHPGFRIGKQGIEKALDLELRGRAGAKKVEVDANGREVRHD
HHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCCC
EDGDIAAVPGKEVQLTLDVDIQNRALEVFGGESGGAVLMDCRTGDILCMMSAPSFDANRF
CCCCEEECCCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCCHHHH
VRGLTGAEYRALANYERRPLLDKCLSGTYPPGSTFKTMTALAALEAGIDPDQTVSCGGGM
HHCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCE
YFGGRYFRCHKKGGHGAQNMRDAIKNSCDVYFYSVASRIGPDRIAAVAREFGFGQTFDIG
EECCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCEEECC
IPGQKKGIVPSTQWVRENRPKDPKWHPGESLSYGIGQGMLTANCLQLAVMTARLANGRKA
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
LNPRLIKSIGGKELPRGSDVPDLPFSQEHLNYVRDGMAAVTEAGGTAFRASQLGLGDILM
CCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEE
AGKTGTAQVYSYKAGGHRGKGGPWRLRDHGLFVAFAPLDEPRYALAVIVQHGMGGSTAAA
ECCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCC
PRAREIMRVALLKDPELRARIETPLPMPEIQPDLELDTVPDSPVFAEPPNTPTPAVGDPL
HHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
VPPSEGPA
CCCCCCCC
>Mature Secondary Structure 
AEPAIFFEEVNERQGLFHRRAVLLGGFAGLGLTALGGRLAYLQLLETQRYEKLATSNQF
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCC
NFKLVPAPRGLIVDRNGAVLAANRPNFRLLVSKDKGTDPEATLKTLASFVPMDEARQRRL
EEEEECCCCCEEEECCCCEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHH
LREISNAPRRAPVSVMEDMTWEQFSAINVRAPELPNVTADMGEVRVYPHGGAFAHVIGYV
HHHHHCCCCCCCHHHHHCCCHHHHCEEEEECCCCCCCCCCCCCEEEEECCCHHHHHHHHH
AKVNREDLTPTGPNSDPILLHPGFRIGKQGIEKALDLELRGRAGAKKVEVDANGREVRHD
HHCCCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCEEECCCCCCEEEEECCCCCCCCCC
EDGDIAAVPGKEVQLTLDVDIQNRALEVFGGESGGAVLMDCRTGDILCMMSAPSFDANRF
CCCCEEECCCCEEEEEEEECCCCCEEEEECCCCCCEEEEEECCCCEEEEEECCCCCHHHH
VRGLTGAEYRALANYERRPLLDKCLSGTYPPGSTFKTMTALAALEAGIDPDQTVSCGGGM
HHCCCCCHHHHHHCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCE
YFGGRYFRCHKKGGHGAQNMRDAIKNSCDVYFYSVASRIGPDRIAAVAREFGFGQTFDIG
EECCEEEEEECCCCCCHHHHHHHHHCCCCEEEEEHHHHCCHHHHHHHHHHHCCCCEEECC
IPGQKKGIVPSTQWVRENRPKDPKWHPGESLSYGIGQGMLTANCLQLAVMTARLANGRKA
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHH
LNPRLIKSIGGKELPRGSDVPDLPFSQEHLNYVRDGMAAVTEAGGTAFRASQLGLGDILM
CCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHCCCCCEEE
AGKTGTAQVYSYKAGGHRGKGGPWRLRDHGLFVAFAPLDEPRYALAVIVQHGMGGSTAAA
ECCCCCEEEEEEECCCCCCCCCCEEEECCCEEEEEECCCCCCCEEEEEECCCCCCCCCCC
PRAREIMRVALLKDPELRARIETPLPMPEIQPDLELDTVPDSPVFAEPPNTPTPAVGDPL
HHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCC
VPPSEGPA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]